One-step cell biomanufacturing platform:porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo

Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.

Bio-inspired Hydroxyapatite/Gelatin Transparent Nanocomposites

Hydroxyapatite(HA)nanoparticles impart outstanding mechanical properties to organicinorganic nanocomposites in bone.Inspired by the composite structure of HA nanoparticles and collagen in bone,a high performance HA/gelatin nanocomposite was first developed.The nanocomposites have much better mechanical properties(elongation at break 29.9%,tensile strength 90.7 MPa,Young’s modulus 5.24 GPa)than pure gelatin films(elongation at break 9.3%,tensile strength 90.8 MPa,Young’s modulus 2.5 GPa).In addition,the composite films keep a high transmittance in visible wavelength range from 0%to 60%of the HA solid content.These differences in properties are attributed to the homogeneous distribution of HA nanoparticles in the gelatin polymer matrix and the strong interaction between the particle surfaces and the gelatin molecules.This protocol should be promising for HA-based nanocomposites with enhanced mechanical properties for biomedical applications.

Three-dimensional porous bimetallic metal–organic framework/gelatin aerogels: A readily recyclable peroxymonosulfate activator for efficient and continuous organic dye removal

As promising catalysts for the degradation of organic pollutants,metal–organic frameworks(MOFs)often face limitations due to the particle agglomeration and challenging recovery in liquid-catalysis application,stemming from their powdery nature.Engineering macroscopic structures from pulverous MOF is thus of great importance for broadening their practical applications.In this study,three-dimensional porous MOF aerogel catalysts were successfully fabricated for degrading organic dyes by activating peroxymonosulfate(PMS).MOF/gelatin aerogel(MOF/GA)catalysts were prepared by directly integrating bimetallic FeCo-BDC with gelatin solutions,followed by freeze-drying and low-temperature calcination.The FeCo-BDC-0.15/GA/PMS system exhibited remarkable performance in degrading various organic dyes,eliminating 99.2%of rhodamine B within a mere 5 min.Compared to the GA/PMS system,there was over a 300-fold increase in the reaction rate constant.Remarkably,high removal efficiency was maintained across varying conditions,including different solution pH,co-existing inorganic anions,and natural water matrices.Radical trapping experiments and electron paramagnetic resonance analysis revealed that the degradation involved radical(SO_(4)^(-)·)and non-radical routes(^(1)O_(2)),of which ^(1)O_(2) was dominant.Furthermore,even after a continuous 400-min reaction in a fixed-bed reactor at a liquid hourly space velocity of 27 h^(-1),the FeCo-BDC/GA composite sustained a degradation efficiency exceeding 98.7%.This work presents highly active MOF-gelatin aerogels for dye degradation and expands the potential for their large-scale,continuous treatment application in organic dye wastewater management.

Constructing a biofunctionalized 3D-printed gelatin/sodium alginate/chitosan tri-polymer complex scaffold with improvised biological andmechanical properties for bone-tissue engineering

Sodium alginate(SA)/chitosan(CH)polyelectrolyte scaffold is a suitable substrate for tissue-engineering application.The present study deals with further improvement in the tensile strength and biological properties of this type of scaffold to make it a potential template for bone-tissue regeneration.We experimented with adding 0%–15%(volume fraction)gelatin(GE),a protein-based biopolymer known to promote cell adhesion,proliferation,and differentiation.The resulting tri-polymer complex was used as bioink to fabricate SA/CH/GEmatrices by three-dimensional(3D)printing.Morphological studies using scanning electron microscopy revealed the microfibrous porous architecture of all the structures,which had a pore size range of 383–419μm.X-ray diffraction and Fourier-transform infrared spectroscopy analyses revealed the amorphous nature of the scaffold and the strong electrostatic interactions among the functional groups of the polymers,thereby forming polyelectrolyte complexes which were found to improve mechanical properties and structural stability.The scaffolds exhibited a desirable degradation rate,controlled swelling,and hydrophilic characteristics which are favorable for bone-tissue engineering.The tensile strength improved from(386±15)to(693±15)kPa due to the increased stiffness of SA/CH scaffolds upon addition of gelatin.The enhanced protein adsorption and in vitro bioactivity(forming an apatite layer)confirmed the ability of the SA/CH/GE scaffold to offer higher cellular adhesion and a bone-like environment to cells during the process of tissue regeneration.In vitro biological evaluation including the MTT assay,confocal microscopy analysis,and alizarin red S assay showed a significant increase in cell attachment,cell viability,and cell proliferation,which further improved biomineralization over the scaffold surface.In addition,SA/CH containing 15%gelatin designated as SA/CH/GE15 showed superior performance to the other fabricated 3D structures,demonstrating its potential for use in bone-tissue engineering.

Fish Gelatin-Based Film Containing Maillard Reaction Products:Properties and Its Use as Bag for Packing Chicken Skin Oil

Maillard reaction is a non-enzymatic browning reaction and its products(MRPs)have been proven to possess antioxidant properties.This research aimed to produce a fish gelatin-based packaging incorporated with MRPs to retard lipid oxidation in chicken skin oil(CSO)during storage at ambient temperature(28℃–30℃).MRPs produced from fish gelatin and fructose(1:1,90℃,pH 11)showed the highest antioxidant properties compared to those prepared under other conditions.Different glycerol/MRPs ratios(30:0,25:5,20:10,15:15,10:20,5:25,0:30)were incorporated into the film and resulting films were characterized.Glycerol/MRPs at 10:20 ratio was chosen to add into the film prior to bag preparation via heat sealing method.CSO packed in the bag was monitored for lipid hydrolysis and oxidation during 15 days of storage(30℃±0.5℃,RH 52%±5%).After 15 days,quality deterioration was lower in CSO packed in the prepared gelatin bag as evidenced by lower FFA,TBARS,and volatile compounds in comparison with CSO packed in LDPE bag.Fish gelatin film added with MRPs possessed an excellent water vapor barrier property(WV-BP).This finding indicated that MRPs could be used to substitute glycerol and simultaneously could serve as antioxidants for the developed active bag.The novel packaging can be a potential alternative packaging for retarding lipid oxidation of lipid or fatty foods.

Studies of XRD and FTIR on Synthesized Novel Hybrid Thin Film Made of Hydroxyapatite, Poly Vinyl Alcohol and Gelatin for Biomedical Application

With the modern advancement of treatment approaches in medical science, the application of biomaterials in tissue engineering provides a remarkable opportunity to overcome graft rejection as well as proper wound healing. In this study, novel hybrid films have been synthesized by incorporation of polyvinyl alcohol (PVA), gelatin, and gelatin with glycerin along with different concentrations of pre-prepared hydroxyapatite (HAP) by solution casting method at room temperature in a biosafety cabinet. Glutaraldehyde has been added as a crosslinker in this whole procedure. Fourier-transform infrared spectroscopy (FTIR), X-Ray Diffraction (XRD) have been conducted to observe and compare the structural and chemical stability of the synthesized hybrid film properties. The FTIR results and X-Ray Diffraction analyses confirmed the chemical interactions between HAP, PVA, gelatin, and glycerin have occurred. The crystallinity of HAP also remains in all the prepared hybrid film samples that are observed in XRD. It is expected that these newly synthesized hybrid films could be a better opportunity for various sectors of tissue engineering such as skin, bone, tendon, and cartilage. These synthesized hybrid films can be suitable for wound healing covering. These studies could be a new scope for long-term drug delivery directly on wound sites in diabetic gangrene foot or burn patients as well as cartilage or joint replacement therapy.

Development and Characterization of Hybrid Film Made of Hydroxyapatite, Poly Vinyl Alcohol and Gelatin for Biomedical Application

In the recent research field of bone tissue engineering, polymeric materials play an implacable role in mimes the natural behavior of hard and soft tissues. In some medical conditions such as diabetics, osteoarthritis, burns, or joint replacement conditions, this polymeric materials implication enhances the internal mechanical activities which result in the early recovery of disease by facilitating the wound healing process. In this study, hybrid films have been synthesized based on polyvinyl alcohol (PVA), gelatin, and gelatin with glycerin incorporated with different concentrations of pre-prepared hydroxyapatite (HAP) by solution casting method at room temperature in biosafety cabinet. Glutaraldehyde has been added as a crosslinker in this whole procedure. The mechanical property, swelling, and porosity percentage have been conducted to characterize the structural stability of the synthesized hybrid films. Porosity and swelling of samples are also represented by proper biocompatibility (>90% porosity and swelling in DDW and PBF vary between 287%~72%). Tensile strength (TS), E modulus (Young’s modulus), Elongation at maximum, and Elongation at break are observed to perceive the mechanical properties of hybrid film samples, which are compatible with mechanical properties of different tissue such as trabecular bone, articular cartilage, tendon, nerve and skin tissue. Though, biocompatibility tests both in vivo and in vitro are essential for clinical application in the future. However, the experiment carried out till now explains the true possibility of newly synthesized hybrid films for long-term drug delivery directly on wound sites for wound healing and burn dressing patients in head-neck surgery reconstruction, diabetic gangrene foot, as well as cartilage or joint replacement therapy.

Tissue Regeneration in Infected Wounds of Albino Rats Using Ciprofloxacin-Loaded Gelatin Microspheres Incorporated Collagen Scaffold: A Histological Approach with H&E Staining

A wound care system consisting of ciprofloxacin-loaded gelatin microspheres impregnated in a macroporous collagen scaffold was created to effectively control wound infection and regenerate soft tissue at the wound site.Histological and biochemical alterations were observed in infected wounds treated with these scaffolds in Albino Wistar rats.Furthermore,the study examined the immediate and prolonged release of ciprofloxacin from the scaffolds,as well as their function in eliminating bacterial infections and expediting the process of skin healing and regeneration.The developed technique was followed in the streamlined process of creating these collagen scaffolds.Compared to untreated wounds,the group receiving scaffold treatment experienced a faster rate of wound closure.It was noted that the rate of infections was considerably reduced and that full soft tissue regeneration occurred within 12 days.The development of well-deposited collagen bundles in the treated groups was demonstrated by H&E staining,which verified the flawless regeneration of the dermis and epidermis.The antimicrobial agent-loaded gelatin microspheres impregnated into the porous collagen scaffold demonstrated remarkable soft tissue regeneration and efficient infection control at the wound site.

BaTiO3/gelatin核壳复合粒子的制备及电场响应性能研究

为了提高BaTiO3颗粒在含水弹性体中的电场响应能力,以粒径约为500nm的单分散球型Ba-TiO3颗粒为基础,采用微乳液法成功制备了BaTiO3为核、明胶(gelatin)为壳的BaTiO3/gelatin核壳复合粒子。利用TEM、XRD、FT-IR、TG和光学接触角测量等手段对粒子的组成、结构及表面亲水性能进行了表征和测量。结果表明,在立方相BaTiO3核的表面均匀包覆了1层约40～50nm的gelatin,Gelatin壳层约占粒子总重的4.0%,且粒子表面保持良好的亲水性。将粒子分散到含水胶体体系中,通过测量在无/有电场作用下胶凝得到的含水弹性体的储能模量考察了粒子在此体系中的电场响应性能,发现BaTiO3/gelat-in复合粒子在明胶(或壳聚糖)水凝胶弹性体中的电场响应性能均明显强于纯BaTiO3粒子,而且在明胶水凝胶中的响应性能更强。结果说明,聚合物包覆能够明显提高BaTiO3粒子的电场响应性能,而且粒子表面与分散体系相容性越高,粒子的响应性能越好。

有氧运动联合KGM对高脂膳食大鼠胰岛素抵抗形成中肝脏Adiponectin/PPARα通路的影响

目的探讨胰岛素抵抗(IR)形成及其运动和KGM干预的机制。方法将6周龄50只雄性SD大鼠随机分为五组:对照组(C组)、高脂饮食组(HF组)、高脂饮食+运动组(HE组)、高脂饮食+葡甘聚糖组(HK组)、高脂饮食+运动训练+葡甘聚糖组(HEK级)。同时将HE、HK、HEK分别进行11 w无负重游泳训练。11 w后测定大鼠空腹血糖(FPG)、空腹胰岛素(FINS)、肝脏胆固醇(TC)、甘油三酯(TG)、脂联素(APN)、APN受体2(AdipoR2)、过氧化物增殖物激活受体α(PPARα)的蛋白含量。结果①HF组大鼠与C组相比,FPG、FINS含量以及胰岛素抵抗指数(HOMAIR)显著升高。②有氧运动和(或)KGM可以降低高脂膳食大鼠FPG水平、FINS以及HOMA-IR,并能显著降低肝脏中TG和TC含量。③与C组相比,HF组大鼠肝脏APN、PPARa含量显著降低,而HF组肝脏AdipoR2含量却升高,而通过双因素方差分析可知,有氧运动和(或)KGM可以显著增加肝脏APN含量和PPARα含量,降低肝脏AdipoR2含量。结论①11 w的高脂饮食可以诱导大鼠IR的形成,而高脂膳食引起的血脂代谢紊乱可能是引起IR产生的重要原因。②有氧运动或补充KGM可能通过改善高脂膳食大鼠肝脏Adiponectin/PPARα信号通路来调节血脂代谢,从而有效预防高脂膳食大鼠IR的形成。同时,有氧运动联合补充KGM对改善高脂大鼠肝脏Adiponectin/PPARα具有交互作用。

魔竽葡苷聚糖凝胶为亲和层析载体与Sepharose 4B的比较研究——Ⅰ.KGM金属螯和层析分离纯化猪血SOD

将KGM凝胶和Sepharose 4B在同样条件下活化偶联,制成Cu^(2+)金属螫合亲和胶,亲和纯化猪血SOD,并对这两种亲和胶的层析效果和性能进行了比较。KGM金属螫合胶对猪血SOD吸附量、纯化倍数、纯化SOD的比活力和回收率分别为53000U/ml胶、19倍、12000U/mg蛋白和94.6％,而Sepharose 4B亲和胶对SOD 的吸附量、纯化倍数、纯化SOD的比活力和回收率分别为79920U/ml胶、11倍、10125U/mg蛋白和95.4％。两种亲和胶所纯化的SOD经聚丙烯酰胺凝胶电泳(PAGE)、活性染色及SDS聚丙烯酰胺凝胶电泳(SDS-PAGE)证明其均为电泳纯。KGM金属螯合胶使用六次后,其对SOD吸附量、去Cu量及SOD的回收率均无明显影响。

纳米ZnS在KGM-AE高分子模板中的可控制备与光限幅性能研究

设计了一种新的乙酸酐改性魔芋葡苷聚糖(KGM-AE)作为高分子模板,通过调节模板剂的改性度、模板剂溶液浓度以及Zn2+离子浓度,探讨了ZnS纳米粒子形成的机理,制备出了大小及形貌可控的纳米ZnS。利用IR、ICP-AES、XRD、TEM等对ZnS结构进行了表征,并测定了纳米ZnS光限幅性能,结果显示纳米ZnS溶液均呈现出明显的光限幅性能。

魔芋葡苷聚糖(KGM)凝胶为亲和层析载体与Sepharose 4B的比较研究 Ⅱ.KGM染料亲和层析分离人血清白蛋白

将 KGM和 Sepharose4 B凝胶在相同条件下活化、偶联接上染料 CibacronBlue F3GA制成了 KGM和 Sepharose 4 B染料亲和吸附剂 ,并用来与牛血清白蛋白( BSA)作用 ,每毫升 KGM亲和吸附剂可吸附 BSA2 8mg,用 Na SCN洗脱时回收为84 .5% ,而 Sepharose 4 B梁料亲和吸附剂每毫升可吸附 BSA 15.3mg,用 Na SCN洗脱时回收为 81.7% ,并对两种凝胶的染料亲和吸附性能进行了比较。用 KGM染料亲和吸附剂分离的人血清白蛋白与人血清白蛋白标准品有同样的纯度 ,都达到了电泳纯。

KGM高温堵剂的研制与性能评价

魔芋葡甘露聚糖（KGM）是魔芋粉的主要成分,是已知植物胶中黏度最大的天然高分子多糖,韧性强、具有独特的凝胶性能。通过组分用量优选得到了KGM堵剂最佳配方：粗品KGM 6.0 g/L＋聚合物HPAM（M=2500×104,HD=25%）1500 mg/L＋增强剂1.0%（体积分数）＋硫脲100 mg/L。该优化配方堵剂热稳定性良好,在110℃下老化60 d后的胶体强度为G级,成胶时间随温度升高而缩短,在90~120℃的性能十分稳定。岩心封堵实验表明,随着堵剂注入量的增加,岩心封堵率提高,最高可达99%以上。对多孔介质的封堵率随堵后注水速度的增大而减小。该堵剂具有可被驱动性,泵入速度增大则突破压力与突破压力梯度越高。岩心注水冲刷50PV后的封堵率大于90%。KGM堵剂只封堵出水层,不封堵出油层,便于现场应用。

KGM/XG复合凝胶的制备及性能研究

[目的]确定KGM/XG复合凝胶的最佳合成条件。[方法]以魔芋葡甘聚糖(KGM)和黄原胶(XG)为主要原料合成了生物可降解复合凝胶KGM/XG,探讨了KGM和XG的共混比例及反应温度对凝胶平衡溶胀比和凝胶强度的影响。[结果]KGM/XG复合凝胶的溶胀比在溶胀初期增加很快,随着溶胀时间的延长,溶胀比增加变慢,最后达到平衡;凝胶的平衡溶胀比随着反应温度的升高逐渐增加,而KGM含量对凝胶平衡溶胀比影响不大;凝胶强度随XG含量的增加和反应温度的升高逐渐增加,当XG含量为70%、反应温度为75℃时,凝胶强度最大。[结论]KGM/XG复合凝胶的最佳合成温度为75℃,XG的适宜含量为70%。

KGM对肥胖大鼠血清脂联素的影响

目的探讨KGM对高脂高糖膳食诱导肥胖大鼠血清脂联素的影响。方法将6周龄30只雄性SD大鼠随机分为三组:对照组(C)、肥胖组(O)、肥胖+葡甘聚糖组(OK)。其中O组OK组喂以高脂高糖膳食,OK组每天下午灌胃魔芋葡甘聚糖(KGM)。每周测量每组大鼠体重,11周后取材测定大鼠血清TC、TG、APN的蛋白含量。结果①与C组相比,O组大鼠体重显著上升,血清TC(P<0.01)和TG(P<0.05)含量均显著升高,血清脂联素水平显著下降(P<0.01)。②KGM可以减轻OK组肥胖大鼠体重,降低肥胖大鼠血清TG(P<0.01)和TC(P<0.01)及脂联素含量(P<0.01)。结论 KGM能显著降低肥胖大鼠血清脂联素水平,改善肥胖症状。

浓硫酸法制备魔芋葡甘聚糖硫酸酯(KGMS)

以魔芋葡甘聚糖(KGM)为研究对象,采用浓硫酸法制备魔芋葡甘聚糖硫酸酯(KGMS)。控制浓硫酸与正丁醇总体积为10mL,并在单因素实验的基础上,选择浓硫酸与正丁醇体积比、反应时间和KGM质量为自变量,KGMS取代度为响应值,利用Box-Benhnken中心组合设计原理和响应面分析法,研究各自变量及其交互作用对取代度的影响,模拟得到回归方程的预测模型。确定最佳制备工艺为:浓硫酸与正丁醇体积比为3.22∶1、反应时间为33min、KGM质量为0.59g。在此条件下制备的KGMS的平均取代度为0.93。通过红外光谱分析表明,经过酯化后KGM在1257cm-1和814cm-1处形成新的吸收峰,为S=O和C-O-S键的特征吸收峰,说明KGM硫酸酯化成功,制备获得的产物为KGMS。

Extraction Characteristics of Anthocyanins from Preliminarily-processed Products of Purple Sweet Potatoes during the Gelatinization Process

Objective] This study aimed to investigate the method for efficient utilization and development of purple sweet potatoes. [Method] Purple sweet potatoes were dried at two specific temperatures and prepared into preliminarily-processed products for gelatinization simulation to analyze the extraction amount of anthocyanins from gelatinized samples at different gelatinization stages. [Result] During the gelatinization process, the extraction rate of anthocyanins from purple sweet potato samples reached the highest as the temperature rised from 90 ℃ to 95 ℃,and the extraction amount of anthocyanins reached the maximum at 15 min postheat preservation at 95 ℃. Purple sweet potato samples dried at 60 ℃ exhibited larger retention amount, larger maximum extraction amount and higher maximum extraction rate of anthocyanins compared with those dried at 110 ℃. [Conclusion] Drying at low temperatures and appropriately shortening the initial gelatinization stage below 90 ℃ is conducive to the retention and extraction of anthocyanins from purple sweet potatoes.

不同静电纺丝膜上骨髓间充质干细胞的黏附、增殖与成血管平滑肌分化

背景:临床上迫切需要小口径人工血管来治疗冠状动脉和外周动脉疾病,目前,血管组织工程已成为制备小口径人工血管的主要方法,选择合适的生物材料和细胞来源是小口径组织工程血管构建成功的关键因素。目的:观察4种静电纺丝膜材料对骨髓间充质干细胞增殖、黏附及分化为血管平滑肌细胞的影响。方法:分离提取SD大鼠骨髓间充质干细胞。将骨髓间充质干细胞分别接种于聚己内酯(PCL)、聚己内酯-透明质酸(PCL-HA)、聚己内酯-丝素蛋白(PCL-SF)、聚己内酯-明胶(PCL-GEL)静电纺丝膜材料上,培养1,3,7 d后,扫描电镜下观察材料上的细胞排布,鬼笔环肽染色观察材料上的细胞增殖与黏附,qRT-PCR检测材料上细胞分泌的CD90、Meflin、转化生长因子βmRNA表达;向血管平滑肌细胞诱导分化7 d后,qRT-PCR检测材料上细胞ɑ-平滑肌肌动蛋白mRNA表达。结果与结论:①扫描电镜下可见骨髓间充质干细胞在4种静电纺丝膜上均沿着静电纺丝膜的纤维走向排列;②鬼笔环肽染色显示,骨髓间充质干细胞在4种静电纺丝膜上分布规律,均沿着纤维走向呈现平行分布,并且PCL-HA、PCL-SF、PCL-GEL静电纺丝膜较PCL静电纺丝膜更有利于骨髓间充质干细胞的增殖、黏附,PCL-SF静电纺丝膜相较于PCL-HA、PCL-GEL静电纺丝膜更有利于骨髓间充质干细胞的增殖、黏附;③qRT-PCR检测显示,4种静电纺丝膜材料均可维持骨髓间充质干细胞CD90和Meflin的mRNA表达,组间比较差异无显著性意义(P>0.05);PCL-HA、PCL-SF、PCL-GEL组培养1,7 d的转化生长因子βmRNA表达高于PCL组(P<0.05),PCL-SF组培养3,7 d的转化生长因子βmRNA表达高于其他3组(P<0.05),PCL-HA组培养7 d的转化生长因子βmRNA表达高于PCL-GEL组(P<0.05);④qRT-PCR检测显示,PCL-SF组ɑ-平滑肌肌动蛋白mRNA表达高于其他3组(P<0.05),PCL-HA组ɑ-平滑肌肌动蛋白mRNA表达高于PCL组(P<0.05);⑤结果表明:相较于PCL、PCL-HA、PCL-GEL静电纺丝膜,PCL-SF静电纺丝膜与骨髓间充质干细胞结合更适合制备小口径组织工程血管。

KGM/明胶/nano HAP椎间盘纤维环组织工程支架的制备与研究

椎间盘退变性疾病已经成为严重影响人们工作和生活的疾病,生物组织工程技术的出现为椎间盘疾病的治疗提供了新的思路和方法。本研究以魔芋葡甘聚糖(Konjac Glucomannan,KGM)、明胶和纳米羟基磷灰石(nano HAP)为原料,分别采用湿法纺丝法和卷膜法构建了椎间盘纤维环组织工程支架。采用扫描电子显微镜(SEM)、X射线衍射仪(XRD)和傅里叶变换红外光谱仪(FT-IR)对支架的成分、结构和形貌进行了分析,并测试了纤维环支架的抗压强度、吸水率、孔隙率、体外降解性及细胞毒性。实验结果表明,得到的椎间盘纤维环组织工程支架具有各向异性的结构特点,与天然椎间盘纤维环结构极为类似,且为多孔结构。支架中加入nano HAP可以提高其强度;湿法纺丝法比卷膜法制备的纤维环支架强度高;戊二醛交联比氨水交联的纤维环支架强度高,但降解速率快;此外,采用戊二醛作交联剂可对nano HAP进行更好的包裹,从而更好地提高支架的强度。支架的吸水率均在700%以上,孔隙率为66%~75%。该研究为研发新型椎间盘纤维环组织工程支架材料提供了一种思路以及一定的实验和理论依据。