An extensively drug-resistant(XDR)Escherichia coli strain 258E was isolated from an anal swab sample of a chicken farm of Anhui province in China.Genomic analyses indicated that the strain 258E harbors an incompatibil...An extensively drug-resistant(XDR)Escherichia coli strain 258E was isolated from an anal swab sample of a chicken farm of Anhui province in China.Genomic analyses indicated that the strain 258E harbors an incompatibility group N(IncN)plasmid pEC258-3,which co-produces bla_(CTX-M-3),bla_(KPC-2),bla_(TEM-1B),qnrS1,aac(6')-Ib-cr,dfrA14,arr-3,and aac(6')-Ib3.Multiple genome arrangement analyses indicated that pEC258-3 is highly homologous with pCRKP-1-KPC discovered in Klebsiella pneumoniae from a patient.Furthermore,conjugation experiments proved that plasmid pEC258-3 can be transferred horizontally and may pose a significant potential threat in animals,community and hospital settings.展开更多
Objective The prevalence of carbapenem-resistant Klebsiella pneumoniae(CR-KP)is a global public health problem.It is mainly caused by the plasmid-carried carbapenemase gene.Outer membrane vesicles(OMVs)contain toxins ...Objective The prevalence of carbapenem-resistant Klebsiella pneumoniae(CR-KP)is a global public health problem.It is mainly caused by the plasmid-carried carbapenemase gene.Outer membrane vesicles(OMVs)contain toxins and other factors involved in various biological processes,includingβ-lactamase and antibiotic-resistance genes.This study aimed to reveal the transmission mechanism of OMV-mediated drug resistance of Klebsiella(K.)pneumoniae.Methods We selected CR-KP producing K.pneumoniae carbapenemase-2(KPC-2)to study whether they can transfer resistance genes through OMVs.The OMVs of CR-KP were obtained by ultracentrifugation,and incubated with carbapenem-sensitive K.pneumoniae for 4 h.Finally,the carbapenem-sensitive K.pneumoniae was tested for the presence of bla_(KPC-2)resistance gene and its sensitivity to carbapenem antibiotics.Results The existence of OMVs was observed by the electron microscopy.The extracted OMVs had bla_(KPC-2)resistance gene.After incubation with OMVs,bla_(KPC-2)resistance gene was detected in sensitive K.pneumoniae,and it became resistant to imipenem and meropenem.Conclusion This study demonstrated that OMVs isolated from KPC-2-producing CR-KP could deliver bla_(KPC-2)to sensitive K.pneumoniae,allowing the bacteria to produce carbapenemase,which may provide a novel target for innovative therapies in combination with conventional antibiotics for treating carbapenem-resistant Enterobacteriaceae.展开更多
目的分析产KPC-2肺炎克雷伯菌ST11株在南京大学附属鼓楼医院重症监护室(Intensive Care Unit,ICU)住院患者胃肠道的定植情况,并分析bla_(KPC-2)阳性肺炎克雷伯菌ST11菌株之间的遗传相关性。方法采集我院ICU患者的肛拭子,使用含有0.5μg...目的分析产KPC-2肺炎克雷伯菌ST11株在南京大学附属鼓楼医院重症监护室(Intensive Care Unit,ICU)住院患者胃肠道的定植情况,并分析bla_(KPC-2)阳性肺炎克雷伯菌ST11菌株之间的遗传相关性。方法采集我院ICU患者的肛拭子,使用含有0.5μg/mL美罗培南的麦康凯平板筛选碳青霉烯耐药肺炎克雷伯菌,K-B法测定其对临床常用抗菌药物的敏感性;采用PCR法和DNA测序技术检测bla_(KPC-2)基因;多位点序列分型技术分析bla_(KPC-2)基因阳性肺炎克雷伯菌的序列分型(sequence type,ST),筛选出ST11菌株。脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)技术分析产bla_(KPC-2)肺炎克雷伯菌ST11菌株之间的遗传相关性。结果共收集肛拭子125份,30株(24.2%)为碳青霉烯耐药肺炎克雷伯菌。其中,27株(90.0%)携带bla_(KPC-2)基因,26株(86.7%)细菌为肺炎克雷伯菌ST11,25株(83.3%)细菌为产KPC-2肺炎克雷伯菌ST11株。PFGE结果显示,19株产KPC-2肺炎克雷伯菌ST11菌株之间有很大的遗传相关性。结论产bla_(KPC-2)肺炎克雷伯菌ST11株在我院ICU患者胃肠道中定植广泛,可能是其感染的主要病原菌,需加强感染控制措施。展开更多
基金the National Key Research and Development Program of China(2018YFE0192600)the Shanghai Agriculture Applied Technology Development Program,China(T20200104)+1 种基金the Fundamental Research Funds for the Central Universities,China(2020JB05)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202203).
文摘An extensively drug-resistant(XDR)Escherichia coli strain 258E was isolated from an anal swab sample of a chicken farm of Anhui province in China.Genomic analyses indicated that the strain 258E harbors an incompatibility group N(IncN)plasmid pEC258-3,which co-produces bla_(CTX-M-3),bla_(KPC-2),bla_(TEM-1B),qnrS1,aac(6')-Ib-cr,dfrA14,arr-3,and aac(6')-Ib3.Multiple genome arrangement analyses indicated that pEC258-3 is highly homologous with pCRKP-1-KPC discovered in Klebsiella pneumoniae from a patient.Furthermore,conjugation experiments proved that plasmid pEC258-3 can be transferred horizontally and may pose a significant potential threat in animals,community and hospital settings.
基金supported by the National Natural Science Foundation of China(No.31771189)the Wuhan Health Commission(No.WX18C17 and No.WX19Q31)the Natural Science Foundation of Hubei Province,China(No.2017CFA065 and No.WJ2019H378).
文摘Objective The prevalence of carbapenem-resistant Klebsiella pneumoniae(CR-KP)is a global public health problem.It is mainly caused by the plasmid-carried carbapenemase gene.Outer membrane vesicles(OMVs)contain toxins and other factors involved in various biological processes,includingβ-lactamase and antibiotic-resistance genes.This study aimed to reveal the transmission mechanism of OMV-mediated drug resistance of Klebsiella(K.)pneumoniae.Methods We selected CR-KP producing K.pneumoniae carbapenemase-2(KPC-2)to study whether they can transfer resistance genes through OMVs.The OMVs of CR-KP were obtained by ultracentrifugation,and incubated with carbapenem-sensitive K.pneumoniae for 4 h.Finally,the carbapenem-sensitive K.pneumoniae was tested for the presence of bla_(KPC-2)resistance gene and its sensitivity to carbapenem antibiotics.Results The existence of OMVs was observed by the electron microscopy.The extracted OMVs had bla_(KPC-2)resistance gene.After incubation with OMVs,bla_(KPC-2)resistance gene was detected in sensitive K.pneumoniae,and it became resistant to imipenem and meropenem.Conclusion This study demonstrated that OMVs isolated from KPC-2-producing CR-KP could deliver bla_(KPC-2)to sensitive K.pneumoniae,allowing the bacteria to produce carbapenemase,which may provide a novel target for innovative therapies in combination with conventional antibiotics for treating carbapenem-resistant Enterobacteriaceae.
文摘目的分析产KPC-2肺炎克雷伯菌ST11株在南京大学附属鼓楼医院重症监护室(Intensive Care Unit,ICU)住院患者胃肠道的定植情况,并分析bla_(KPC-2)阳性肺炎克雷伯菌ST11菌株之间的遗传相关性。方法采集我院ICU患者的肛拭子,使用含有0.5μg/mL美罗培南的麦康凯平板筛选碳青霉烯耐药肺炎克雷伯菌,K-B法测定其对临床常用抗菌药物的敏感性;采用PCR法和DNA测序技术检测bla_(KPC-2)基因;多位点序列分型技术分析bla_(KPC-2)基因阳性肺炎克雷伯菌的序列分型(sequence type,ST),筛选出ST11菌株。脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)技术分析产bla_(KPC-2)肺炎克雷伯菌ST11菌株之间的遗传相关性。结果共收集肛拭子125份,30株(24.2%)为碳青霉烯耐药肺炎克雷伯菌。其中,27株(90.0%)携带bla_(KPC-2)基因,26株(86.7%)细菌为肺炎克雷伯菌ST11,25株(83.3%)细菌为产KPC-2肺炎克雷伯菌ST11株。PFGE结果显示,19株产KPC-2肺炎克雷伯菌ST11菌株之间有很大的遗传相关性。结论产bla_(KPC-2)肺炎克雷伯菌ST11株在我院ICU患者胃肠道中定植广泛,可能是其感染的主要病原菌,需加强感染控制措施。
