目的探讨乳腺癌摄取^(99)Tc^(m)-甲氧异腈(MIBI)与Ki-67抗原表达的关系,并验证Ki-67预测新辅助化疗(NAC)疗效的价值。方法回顾性分析2018年1月至2020年3月于宁夏医科大学总医院就诊的47例乳腺癌患者NAC前后双时相^(99)Tc^(m)-MIBI SPECT...目的探讨乳腺癌摄取^(99)Tc^(m)-甲氧异腈(MIBI)与Ki-67抗原表达的关系,并验证Ki-67预测新辅助化疗(NAC)疗效的价值。方法回顾性分析2018年1月至2020年3月于宁夏医科大学总医院就诊的47例乳腺癌患者NAC前后双时相^(99)Tc^(m)-MIBI SPECT/CT断层融合显像,分析T/N比值和肿瘤大小在治疗前后的变化及与Ki-67之间的关系。采用两样本t检验、直线相关性及χ^(2)检验分析数据。结果乳腺癌^(99)Tc^(m)-MIBI显像NAC前后20 min T/N比值、2 h T/N比值下降差异有统计意义(P<0.05),肿瘤NAC化疗前后最大径缩小差异有统计学意义(P<0.05);Ki-67高表达组与低表达组患者NAC后T/N比值下降和肿瘤缩小差异均有统计学意义(P<0.05);Ki-67的表达与20 min和2 h T/N比值有相关性(P<0.05)。结论Ki-67抗原表达和乳腺^(99)Tc^(m)-MIBI显像有助于了解肿瘤增殖,Ki-67抗原表达对NAC疗效有预测价值,可用于指导临床治疗。展开更多
BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and sh...BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be consi- dered for palliative treatment such as chemotherapy and ra- diotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carci- noma were cultured by the cell culture technique. The ex- periment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of prolifera- tion-related gene proteins PCNA and Ki-67 of human gall- bladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose- and time-dependent manner, with the IC50 value of 56.18 μg/ ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932 ±0.031 vs. 0.318 ±0.023, P<0.001) and Ki-67 (0.964 ±0.092 vs. 0.297 ±0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expres- sion of their proliferation-related gene proteins PCNA and Ki-67.展开更多
Objective： Colorectal cancer is one of the major contributors to cancer death worldwide. Lack of reliable colorectal cancer markers has hampered the management of these cancer patients. Our main purpose was to study ...Objective： Colorectal cancer is one of the major contributors to cancer death worldwide. Lack of reliable colorectal cancer markers has hampered the management of these cancer patients. Our main purpose was to study the correlation between histopathological variables of colorectal adenocarcinomas and identify histopathological markers that are of prognostic value in patients with colorectal cancer. Methods： In the present study, we examined the expression of carcinoembryonic antigen （CEA）, p53, Ki-67 and glutathion Stransferase （GST） -n by using immunohistochemical staining methods in 126 colorectal carcinoma patients and evaluated the lymph node metastasis status in these patients by histopathological examination. Results： The positive rates of CEA, p53, Ki-67 and GST-π expression in the colorectal cancer tissue specimens examined were 95.23%, 55.56%, 53.38% and 82.30%, respectively. Expression of p53 and Ki-67 was significantly correlated with the Dukes stages of the tumor, with higher levels of these proteins in Dukes＇ C and D tumors than those in Dukes＇ A and B tumors. Furthermore, the expression of p53, GST-π and Ki-67 correlated with prognosis of patients with colorectal cancer. Additionally, the expression of p53 in colorectal cancer was closely related to the expression of Ki-67 and the expression of GST-π was directly correlated with that of p53. Conclusion： The expression of CEA, p53, Ki-67 and GST-π was correlated with various clinical features of patients with colorectal cancer. The combined use of these histopathological markers appeared to be a promising tool in predicting the prognosis of patients with this type of cancer.展开更多
文摘目的探讨乳腺癌摄取^(99)Tc^(m)-甲氧异腈(MIBI)与Ki-67抗原表达的关系,并验证Ki-67预测新辅助化疗(NAC)疗效的价值。方法回顾性分析2018年1月至2020年3月于宁夏医科大学总医院就诊的47例乳腺癌患者NAC前后双时相^(99)Tc^(m)-MIBI SPECT/CT断层融合显像,分析T/N比值和肿瘤大小在治疗前后的变化及与Ki-67之间的关系。采用两样本t检验、直线相关性及χ^(2)检验分析数据。结果乳腺癌^(99)Tc^(m)-MIBI显像NAC前后20 min T/N比值、2 h T/N比值下降差异有统计意义(P<0.05),肿瘤NAC化疗前后最大径缩小差异有统计学意义(P<0.05);Ki-67高表达组与低表达组患者NAC后T/N比值下降和肿瘤缩小差异均有统计学意义(P<0.05);Ki-67的表达与20 min和2 h T/N比值有相关性(P<0.05)。结论Ki-67抗原表达和乳腺^(99)Tc^(m)-MIBI显像有助于了解肿瘤增殖,Ki-67抗原表达对NAC疗效有预测价值,可用于指导临床治疗。
文摘BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be consi- dered for palliative treatment such as chemotherapy and ra- diotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carci- noma were cultured by the cell culture technique. The ex- periment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of prolifera- tion-related gene proteins PCNA and Ki-67 of human gall- bladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose- and time-dependent manner, with the IC50 value of 56.18 μg/ ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932 ±0.031 vs. 0.318 ±0.023, P<0.001) and Ki-67 (0.964 ±0.092 vs. 0.297 ±0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expres- sion of their proliferation-related gene proteins PCNA and Ki-67.
基金supported by the Natural Sciences Basic Research Project,Education(08KJD310005)Department of Jiangsu Provincethe 5th"Six Talent-Person-Peak-Program",Jiangsu Province,ChinaThe progress main program fund of Nanjing Medical University 2007(nmu07002)
文摘Objective： Colorectal cancer is one of the major contributors to cancer death worldwide. Lack of reliable colorectal cancer markers has hampered the management of these cancer patients. Our main purpose was to study the correlation between histopathological variables of colorectal adenocarcinomas and identify histopathological markers that are of prognostic value in patients with colorectal cancer. Methods： In the present study, we examined the expression of carcinoembryonic antigen （CEA）, p53, Ki-67 and glutathion Stransferase （GST） -n by using immunohistochemical staining methods in 126 colorectal carcinoma patients and evaluated the lymph node metastasis status in these patients by histopathological examination. Results： The positive rates of CEA, p53, Ki-67 and GST-π expression in the colorectal cancer tissue specimens examined were 95.23%, 55.56%, 53.38% and 82.30%, respectively. Expression of p53 and Ki-67 was significantly correlated with the Dukes stages of the tumor, with higher levels of these proteins in Dukes＇ C and D tumors than those in Dukes＇ A and B tumors. Furthermore, the expression of p53, GST-π and Ki-67 correlated with prognosis of patients with colorectal cancer. Additionally, the expression of p53 in colorectal cancer was closely related to the expression of Ki-67 and the expression of GST-π was directly correlated with that of p53. Conclusion： The expression of CEA, p53, Ki-67 and GST-π was correlated with various clinical features of patients with colorectal cancer. The combined use of these histopathological markers appeared to be a promising tool in predicting the prognosis of patients with this type of cancer.
