KiSS-1,first identified as an anti-metastasis gene in melanoma,encodes C-terminally amidated peptide products,including kisspeptin-145,kisspeptin-54,kisspeptin-14,kisspeptin-13 and kisspeptin-10.These products are end...KiSS-1,first identified as an anti-metastasis gene in melanoma,encodes C-terminally amidated peptide products,including kisspeptin-145,kisspeptin-54,kisspeptin-14,kisspeptin-13 and kisspeptin-10.These products are endogenous ligands coupled to G protein-coupled receptor 54(GPR54)/hOT7T175/AXOR12.To date,the regulatory activities of the KiSS-1/GPR54 system,such as puberty initiation,antitumor metastasis,fertility in adulthood,hypothalamic-pituitary-gonadal axis(HPG axis)feedback,and trophoblast invasion,have been investigated intensively.Accumulating evidence has demonstrated that KiSS-1 played a key role in reproduction and served as a promising biomarker relative to the diagnosis,identification of therapeutic targets and prognosis in various carcinomas,while few studies have systematically summarized its subjective factors and concluded the functions of KiSS-1/GPR54 signaling in physiology homeostasis and cancer biology.In this review,we retrospectively summarized the regulators of the KiSS-1/GPR54 system in different animal models and reviewed its functions according to physiological homeostasis regulations and above all,cancer biology,which provided us with a profound understanding of applying the KiSS-1/GPR54 system into medical applications.展开更多
Objective:To explore the effect of wujiadan Recipe on the expression of FSH,E2 in serum and KiSS-1,GPR54,GnRH mRNA in hypothalamic arcuate nucleus tissue homogenate of ovariectomized rats,and to further explore the me...Objective:To explore the effect of wujiadan Recipe on the expression of FSH,E2 in serum and KiSS-1,GPR54,GnRH mRNA in hypothalamic arcuate nucleus tissue homogenate of ovariectomized rats,and to further explore the mechanism of wujiadan Recipe on menopausal syndrome.Methods:Sixty-four adult female rats were randomly divided into three groups:blank group,model group,Chinese medicine control group,western medicine control group,and wujiadan high,medium and low dose groups.Except for the blank group,all the other groups were ovariectomized.The rats in the normal group and the model group were given the same dose of saline once a day.One rat in the blank group died during the experiment.After 4 weeks of continuous administration,blood was collected from abdominal aorta and brain stereotaxic localization was performed to preserve the arcuate nucleus of hypothalamus.The levels of FSH and E2 in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of Kiss-1,GPR54 and GnRH mRNA in arcuate nucleus of hypothalamus were detected by PCR.Results:1.Compared with the blank group,FSH level in the model group was significantly higher(P<0.01),and E2 level was significantly lower(P<0.01).Compared with the model group,the level of serum E2 in the western medicine control group increased significantly(P.The serum FSH content in each group decreased,especially in the high dose group and the western medicine control group(P<0.01),and in the Chinese medicine control group,the middle dose group and the low dose group(P<0.05).Compared with the blank group,the expression of KiSS-1 mRNA,GPR54 mRNA and GnRH mRNA in the model group increased significantly(P<0.01);compared with the model group,the expression of KiSS-1 mRNA and GPR54 mRNA in the high dose group,middle dose group and Western medicine control group of wujiadan prescription decreased significantly(P<0.05).Compared with the model group,the expression of GnRH mRNA in the high dose group and the western medicine control group decreased significantly(P<0.01);the middle dose group of wujiadan decreased significantly(P<0.05).Conclusion:Wujiadan recipe can improve the serum sex hormone level of castrated rats,and reduce the over expression of KiSS-1,GPR54 and GnRH mRNA in hypothalamic arcuate nucleus of castrated rats,which may be the reproductive endocrine mechanism of wujiadan recipe in the treatment of menopausal syndrome.展开更多
基金This work was supported by National Natural Science Foundation of China(No.81902754 to LD,81772880 to YN and 81702680 to YS)China Postdoctoral Science Foundation(No.2019M651789 to LD)+2 种基金Fundamental Research Funds for the Central Universities(No.021014380161 to LD)Natural Science Foundation of Jiangsu Province(No.BK20190304 to LD)Nanjing Medical Science and Technology Development Foundation,Nanjing Department of Health(No.YKK17138 to YS).
文摘KiSS-1,first identified as an anti-metastasis gene in melanoma,encodes C-terminally amidated peptide products,including kisspeptin-145,kisspeptin-54,kisspeptin-14,kisspeptin-13 and kisspeptin-10.These products are endogenous ligands coupled to G protein-coupled receptor 54(GPR54)/hOT7T175/AXOR12.To date,the regulatory activities of the KiSS-1/GPR54 system,such as puberty initiation,antitumor metastasis,fertility in adulthood,hypothalamic-pituitary-gonadal axis(HPG axis)feedback,and trophoblast invasion,have been investigated intensively.Accumulating evidence has demonstrated that KiSS-1 played a key role in reproduction and served as a promising biomarker relative to the diagnosis,identification of therapeutic targets and prognosis in various carcinomas,while few studies have systematically summarized its subjective factors and concluded the functions of KiSS-1/GPR54 signaling in physiology homeostasis and cancer biology.In this review,we retrospectively summarized the regulators of the KiSS-1/GPR54 system in different animal models and reviewed its functions according to physiological homeostasis regulations and above all,cancer biology,which provided us with a profound understanding of applying the KiSS-1/GPR54 system into medical applications.
基金Scientific research project of Heilongjiang Provincial Health and Health Commission(No.2019-085)
文摘Objective:To explore the effect of wujiadan Recipe on the expression of FSH,E2 in serum and KiSS-1,GPR54,GnRH mRNA in hypothalamic arcuate nucleus tissue homogenate of ovariectomized rats,and to further explore the mechanism of wujiadan Recipe on menopausal syndrome.Methods:Sixty-four adult female rats were randomly divided into three groups:blank group,model group,Chinese medicine control group,western medicine control group,and wujiadan high,medium and low dose groups.Except for the blank group,all the other groups were ovariectomized.The rats in the normal group and the model group were given the same dose of saline once a day.One rat in the blank group died during the experiment.After 4 weeks of continuous administration,blood was collected from abdominal aorta and brain stereotaxic localization was performed to preserve the arcuate nucleus of hypothalamus.The levels of FSH and E2 in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of Kiss-1,GPR54 and GnRH mRNA in arcuate nucleus of hypothalamus were detected by PCR.Results:1.Compared with the blank group,FSH level in the model group was significantly higher(P<0.01),and E2 level was significantly lower(P<0.01).Compared with the model group,the level of serum E2 in the western medicine control group increased significantly(P.The serum FSH content in each group decreased,especially in the high dose group and the western medicine control group(P<0.01),and in the Chinese medicine control group,the middle dose group and the low dose group(P<0.05).Compared with the blank group,the expression of KiSS-1 mRNA,GPR54 mRNA and GnRH mRNA in the model group increased significantly(P<0.01);compared with the model group,the expression of KiSS-1 mRNA and GPR54 mRNA in the high dose group,middle dose group and Western medicine control group of wujiadan prescription decreased significantly(P<0.05).Compared with the model group,the expression of GnRH mRNA in the high dose group and the western medicine control group decreased significantly(P<0.01);the middle dose group of wujiadan decreased significantly(P<0.05).Conclusion:Wujiadan recipe can improve the serum sex hormone level of castrated rats,and reduce the over expression of KiSS-1,GPR54 and GnRH mRNA in hypothalamic arcuate nucleus of castrated rats,which may be the reproductive endocrine mechanism of wujiadan recipe in the treatment of menopausal syndrome.
