hronic rejection is the main factor to result in the loss of renal allograft In order to look for a potential therapy chronic rejection, we investigated the efficacy of estradiol on preventing renal chronic rejectio...hronic rejection is the main factor to result in the loss of renal allograft In order to look for a potential therapy chronic rejection, we investigated the efficacy of estradiol on preventing renal chronic rejection The kidneys of female F344 rats were orthotopically transplanted into ovatiectomized female Lewis rats and treated for 16 weeks with either estradiol or vehicle Compared with controls treated with vehicle, estradiol treatment reduced urinary protein excretion, glomerular sclerosis, interstitial infiltration and fibrosis, vascular lesions, in parallel to a reduced ICAM 1 and TGF β mRNA expression Our results suggested that estrodiol could significantly decrease the progression of chronic rejection, at least in female recipients, and the reduced adhesion molecule and TGF β gene expression may be involved in the mechanism for estradiol to prevent chronic rejection展开更多
Background AIIogeneic transplant rejection is currently a major problem encountered during organ transplantation. The dendritic cell （DC） is the most effective powerful known professional antigen-presenting cell, an...Background AIIogeneic transplant rejection is currently a major problem encountered during organ transplantation. The dendritic cell （DC） is the most effective powerful known professional antigen-presenting cell, and recent studies have found that DCs can also induce immune tolerance, and avoid or reduce the degree of transplant rejection. The aim of this study was to evaluate the effect of transfused immature CD4~ DCs on renal allografts in the rat model. Methods In this study, we induced CD4~ immature DCs from rat bone marrow cells by a cytokine cocktail. The immature CD4~ DCs were identified by morphological analysis and then the suppressive activity of these cells conditioned with donor kidney antigen was evaluated in vitro and in vivo. Results Immature CD4~ DCs conditioned with donor kidney antigen possessed immunosuppressive activity in vitro and they were able to prolong renal transplant survival in an allograft rat model in vivo. Conclusions Our study provides new information on efficacious renal transplantation, which might be useful for understanding the function of immature CD4~ DCs in modulating renal transplant rejection and improving clinical outcome in future studies.展开更多
文摘hronic rejection is the main factor to result in the loss of renal allograft In order to look for a potential therapy chronic rejection, we investigated the efficacy of estradiol on preventing renal chronic rejection The kidneys of female F344 rats were orthotopically transplanted into ovatiectomized female Lewis rats and treated for 16 weeks with either estradiol or vehicle Compared with controls treated with vehicle, estradiol treatment reduced urinary protein excretion, glomerular sclerosis, interstitial infiltration and fibrosis, vascular lesions, in parallel to a reduced ICAM 1 and TGF β mRNA expression Our results suggested that estrodiol could significantly decrease the progression of chronic rejection, at least in female recipients, and the reduced adhesion molecule and TGF β gene expression may be involved in the mechanism for estradiol to prevent chronic rejection
基金This study was supported by the grants from the National Natural Science Foundation of China （No. 81000230） and Science and Technology Projects in Guangdong Province （No. 2010B031600052 and No. 2011B040300021）.
文摘Background AIIogeneic transplant rejection is currently a major problem encountered during organ transplantation. The dendritic cell （DC） is the most effective powerful known professional antigen-presenting cell, and recent studies have found that DCs can also induce immune tolerance, and avoid or reduce the degree of transplant rejection. The aim of this study was to evaluate the effect of transfused immature CD4~ DCs on renal allografts in the rat model. Methods In this study, we induced CD4~ immature DCs from rat bone marrow cells by a cytokine cocktail. The immature CD4~ DCs were identified by morphological analysis and then the suppressive activity of these cells conditioned with donor kidney antigen was evaluated in vitro and in vivo. Results Immature CD4~ DCs conditioned with donor kidney antigen possessed immunosuppressive activity in vitro and they were able to prolong renal transplant survival in an allograft rat model in vivo. Conclusions Our study provides new information on efficacious renal transplantation, which might be useful for understanding the function of immature CD4~ DCs in modulating renal transplant rejection and improving clinical outcome in future studies.
