The paper was to explore the preventive effects of five drugs on mycoplasma pneumonia of swine （MPS） and to provide reference for clinical medication of pig farms in Hainan Province. A total of 444 health piglets we...The paper was to explore the preventive effects of five drugs on mycoplasma pneumonia of swine （MPS） and to provide reference for clinical medication of pig farms in Hainan Province. A total of 444 health piglets were randomly divided into 6 groups, including five medication groups （72 piglets in group A, 74 pig- lets in group B, 72 piglets in group C, 76 piglets in group D, 76 piglets in group E） and one control group （74 piglets）. The piglets in experimental groups were treated drugs once a day for successive 5 days at 30, 60, 90, 120 and 150 of age. The piglets in control group were free of medication. At 70 and 140 days of age, 15 piglets of each group were randomly selected to collect their blood sermn. The Mycoplasma hyopneumoniae （M-Hyo） antibodies in serum were measured by en- zyme-linked immunosorbent assay （ELISA）. During the experiment, the incidence rates of respiratory disease, lung lesion, feed conversion rate, average daily gain （ADG）, and mortality rate of pigs were also observed and recorded. The results showed that the five drugs had significant difference in preventative effects. Group C （Zhiyuanjing group） received the best preventive effect and the highest economic benefits. Compared with control group, the ADG and feed conversion rate in group C were increased by 7.53% and 9.09%, respectively; the incidence rate of respiratory disease was reduced by 13.44% and lung lesion was alleviated by 81.43% ; and the earnings of each pig could rise by 132.70 yuan. The preventative effect and economic benefit of the drugs was sequenced by Chansu Kechuanling and Bingchan Kechuanwang. Wante Feilin and amoxicillin had weaker preventive effects against MPS but greatly influenced growth performance of pigs, so they should be used alternatively with other drugs.展开更多
Objective:To analyze the clinical distribution and drug resistance of Klebsiella pneumoniae isolated from patients in a certain hospital and provide a basis for the rational use of antibiotics in the clinical treatmen...Objective:To analyze the clinical distribution and drug resistance of Klebsiella pneumoniae isolated from patients in a certain hospital and provide a basis for the rational use of antibiotics in the clinical treatment for the infection of Klebsiella pneumoniae.Methods:1,192 strains of Klebsiella pneumoniae isolated from clinical specimens from 2012 to 2016 were collected.The strains were identified by VITEK-2 Compact Microbiological Identification System,and the corresponding results of the antimicrobial susceptibility test were interpreted in accordance with the standards recommended by Clinical and Laboratory Standards Institute(CLSI).Results:1,192 strains of Klebsiella pneumoniae were mainly isolated from sputum(65.6%),and most of them were from Respiratory Medicine Department and Medical Intensive Care Unit of Respiratory Medicine Department(MICU),accounting for 41.4%.Out of 1,192 strains,448 strains were detected to produce extended-spectrum beta-lactamases(ESBLs),accounting for 37.6%.In addition,the detection rates of ESBL-producing Klebsiella pneumoniae for 5 consecutive years showed an increasing trend year by year,and they were higher than the national average values published by China Antimicrobial Resistance Surveillance System(CARSS)in the corresponding period.The drug resistance rate of ESBL-producing Klebsiella pneumoniae was significantly higher than that of non ESBL-producing strains.Conclusions:The infection caused by Klebsiella pneumoniae mainly occurs in the lower respiratory tract,and the drug resistance rates of Klebsiella pneumoniae to antibiotics in the drug susceptibility spectrum are maintained at a high level.Therefore,the rational selection of antibiotics for the clinical treatment of lower respiratory tract infection caused by Klebsiella pneumoniae must be based on the production of ESBLs and the results of antimicrobial susceptibility test.展开更多
Objective To characterize carbapenem （CPM）-non-susceptible Klebsiella pneumoniae （K. pneumoniae） and carbape-nemase produced by these strains isolated from Beijing Children＇s Hospital based on a five-year surveil...Objective To characterize carbapenem （CPM）-non-susceptible Klebsiella pneumoniae （K. pneumoniae） and carbape-nemase produced by these strains isolated from Beijing Children＇s Hospital based on a five-year surveillance. Methods The Minimal Inhibition Concentration values for 15 antibiotics were assessed using the Phonixl00 compact system. PCR amplification and DNA sequencing were used to detect genes encoding carbapenemases. WHONET 5.6 was finally used for resistance analysis. Results In total, 179 strains of CPM-non-susceptible K. pneumoniae were isolated from January, 2010 to December, 2014. The rates of non-susceptible to imipenem and meropenem were 95.0% and 95.6%, respectively. In the 179 strains, 95 （53.1%） strains carried the blalMP gene, and IMP-4 and IMP-8 were detected in 92 （96.8%） and 3 （3.2%） IMP-producing isolates, respectively. 65 （36.3%） strains carried the blaNDM_1 gene. 6 （3.4%） strains carried the blaKpc gene, and KPC-2 were detected in 6 KPC-producing isolates. In addition, New Delhi-Metallo-1 （NDM-1） producing isolates increased from 7.1% to 63.0% in five years and IMP-4 producing isolates decreased from 75.0% to 28.3%. Conclusion High frequencies of multiple resistances to antibiotics were observed in the CPM-non-susceptible K. pneumoniae strains isolated from Beijing Children＇s Hospital. The production of IMP-4 and NDM-1 metallo-13-1actamases appears to be an important mechanism for CPM-non- susceptible in K. pneumoniae.展开更多
The study was conducted on new-born babies in whom septicemia was suspected, to determine the prevalence of bacterial strains isolated and their sensitivity to antimicrobial drugs. The study was carried out at La Paz ...The study was conducted on new-born babies in whom septicemia was suspected, to determine the prevalence of bacterial strains isolated and their sensitivity to antimicrobial drugs. The study was carried out at La Paz Medical Center, Microbiology section, Malabo, Equatorial Guinea from August 2013 to October 2015. Out of 293 septicemia suspected cases, 29 (10%) blood cultures were positive, 28 with bacterial growth and 1 with growth of Candida sp. The mortality rate of neonates caused by Gram negative bacterial sepsis was 34.7%. Among the Gram negative bacteria (24 isolates), the most common types were Klebsiella pneumoniae (16 = 69.6%), followed by Escherichia coli (4 = 17.4%) and Acinetobacter species (4 = 17.4%). Four Gram positive bacteria were also isolated and identified all ascoagulase-negative staphylococci. All the Klebsiella pneumoniae isolates and Acinetobacter species demonstrated Multi Drug Resistance against different antibiotics with Extended-spectrum β-lactamase (ESBL) activity. The most frequent causative agent of bacterial sepsis in new-born children was Klebsiella pneumoniae. An alarming level of Multi Drug Resistance (MDR) Klebsiella pneumoniae strains to the first choice antibiotic treatment was observed.展开更多
Klebsiella pneumoniae(K.pneumoniae)is one of the main gram-negative bacilli in clini- cal practice.Nosocomial infections caused by K.pneumoniae producing extended-spectrumβ-lactama- ses(ESBLs)are very difficult to tr...Klebsiella pneumoniae(K.pneumoniae)is one of the main gram-negative bacilli in clini- cal practice.Nosocomial infections caused by K.pneumoniae producing extended-spectrumβ-lactama- ses(ESBLs)are very difficult to treat.This paper investigated the resistant characteristics of K.pneu- moniae producing ESBLs and their aminoglycoside-modifying enzyme gene expressions including N- acetyhransferases and O-adenyhransfemses.Bacteria identification and ESBLs confirmatory tests were performed by Phoenix^(TM)-100 system.And minimum inhibitory concentrations(MICs)of gentamicin, amikacin,kanamycin,tobramycin,netilmicin and neomycin in 53 K.pneumoniae isolates were de- tected by agar dilution.In addition,six aminoglycoside-modifying enzyme genes were amplified by polymerase chain reaction(PCR)and verified by DNA sequencer.It was found that imipenem and meropenem against 120 K.pneumoniae isolates produced powerful antimicrobial activities.The resis- tant rates of gentamicin and amikacin were 55.0% and 46.7%,respectively.Except neomycin, MIC_(50)and MIC_(90)of gentamicin,amikacin,kanamycin,tobramycin and netilmicin in 53 K.pneumoni- ae were all>128μg/ml,and the resistant rates were 83.0%,52.3%,75.5%,81.1% and 69.8%,respectively.However,neomycin was only 39.6%.In addition,five modifying enzyme genes,including aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6')-Ⅰb,ant(3″)-Ⅰ,ant(2″)-Ⅰgenes,were found in 53 isoahes except aac(6')-Ⅱ,and their positive rates were 11.3%,67.9%,47.2%, 1.9% and 39.6%,respectively.It was also confirmed by nucleotide sequence analysis that the above resistant genes shared nearly 100% identities with GenBank published genes.The results obtained in the present study indicated that K.pneumoniae producing ESBLs strains are rapidly spreading in our hospital,and their resistance to aminoglycosides may be associated with aminoglycoside-modifying enz- yme gene expressions.展开更多
基金National Natural Science Foundation of China(31560696)Special Project of Enterprises-Universities-Researches Integration of Hainan Province(cxy20150008)Special Project of Technology Development in Scientific Research Institutes of Hainan Province(KYYS-2014-32)
文摘The paper was to explore the preventive effects of five drugs on mycoplasma pneumonia of swine （MPS） and to provide reference for clinical medication of pig farms in Hainan Province. A total of 444 health piglets were randomly divided into 6 groups, including five medication groups （72 piglets in group A, 74 pig- lets in group B, 72 piglets in group C, 76 piglets in group D, 76 piglets in group E） and one control group （74 piglets）. The piglets in experimental groups were treated drugs once a day for successive 5 days at 30, 60, 90, 120 and 150 of age. The piglets in control group were free of medication. At 70 and 140 days of age, 15 piglets of each group were randomly selected to collect their blood sermn. The Mycoplasma hyopneumoniae （M-Hyo） antibodies in serum were measured by en- zyme-linked immunosorbent assay （ELISA）. During the experiment, the incidence rates of respiratory disease, lung lesion, feed conversion rate, average daily gain （ADG）, and mortality rate of pigs were also observed and recorded. The results showed that the five drugs had significant difference in preventative effects. Group C （Zhiyuanjing group） received the best preventive effect and the highest economic benefits. Compared with control group, the ADG and feed conversion rate in group C were increased by 7.53% and 9.09%, respectively; the incidence rate of respiratory disease was reduced by 13.44% and lung lesion was alleviated by 81.43% ; and the earnings of each pig could rise by 132.70 yuan. The preventative effect and economic benefit of the drugs was sequenced by Chansu Kechuanling and Bingchan Kechuanwang. Wante Feilin and amoxicillin had weaker preventive effects against MPS but greatly influenced growth performance of pigs, so they should be used alternatively with other drugs.
文摘Objective:To analyze the clinical distribution and drug resistance of Klebsiella pneumoniae isolated from patients in a certain hospital and provide a basis for the rational use of antibiotics in the clinical treatment for the infection of Klebsiella pneumoniae.Methods:1,192 strains of Klebsiella pneumoniae isolated from clinical specimens from 2012 to 2016 were collected.The strains were identified by VITEK-2 Compact Microbiological Identification System,and the corresponding results of the antimicrobial susceptibility test were interpreted in accordance with the standards recommended by Clinical and Laboratory Standards Institute(CLSI).Results:1,192 strains of Klebsiella pneumoniae were mainly isolated from sputum(65.6%),and most of them were from Respiratory Medicine Department and Medical Intensive Care Unit of Respiratory Medicine Department(MICU),accounting for 41.4%.Out of 1,192 strains,448 strains were detected to produce extended-spectrum beta-lactamases(ESBLs),accounting for 37.6%.In addition,the detection rates of ESBL-producing Klebsiella pneumoniae for 5 consecutive years showed an increasing trend year by year,and they were higher than the national average values published by China Antimicrobial Resistance Surveillance System(CARSS)in the corresponding period.The drug resistance rate of ESBL-producing Klebsiella pneumoniae was significantly higher than that of non ESBL-producing strains.Conclusions:The infection caused by Klebsiella pneumoniae mainly occurs in the lower respiratory tract,and the drug resistance rates of Klebsiella pneumoniae to antibiotics in the drug susceptibility spectrum are maintained at a high level.Therefore,the rational selection of antibiotics for the clinical treatment of lower respiratory tract infection caused by Klebsiella pneumoniae must be based on the production of ESBLs and the results of antimicrobial susceptibility test.
基金supported by Scientific Research Project of Beijing Children's Hospital(2012MS08)Beijing Municipal Science and Technology Project(D131100005313014)
文摘Objective To characterize carbapenem （CPM）-non-susceptible Klebsiella pneumoniae （K. pneumoniae） and carbape-nemase produced by these strains isolated from Beijing Children＇s Hospital based on a five-year surveillance. Methods The Minimal Inhibition Concentration values for 15 antibiotics were assessed using the Phonixl00 compact system. PCR amplification and DNA sequencing were used to detect genes encoding carbapenemases. WHONET 5.6 was finally used for resistance analysis. Results In total, 179 strains of CPM-non-susceptible K. pneumoniae were isolated from January, 2010 to December, 2014. The rates of non-susceptible to imipenem and meropenem were 95.0% and 95.6%, respectively. In the 179 strains, 95 （53.1%） strains carried the blalMP gene, and IMP-4 and IMP-8 were detected in 92 （96.8%） and 3 （3.2%） IMP-producing isolates, respectively. 65 （36.3%） strains carried the blaNDM_1 gene. 6 （3.4%） strains carried the blaKpc gene, and KPC-2 were detected in 6 KPC-producing isolates. In addition, New Delhi-Metallo-1 （NDM-1） producing isolates increased from 7.1% to 63.0% in five years and IMP-4 producing isolates decreased from 75.0% to 28.3%. Conclusion High frequencies of multiple resistances to antibiotics were observed in the CPM-non-susceptible K. pneumoniae strains isolated from Beijing Children＇s Hospital. The production of IMP-4 and NDM-1 metallo-13-1actamases appears to be an important mechanism for CPM-non- susceptible in K. pneumoniae.
文摘The study was conducted on new-born babies in whom septicemia was suspected, to determine the prevalence of bacterial strains isolated and their sensitivity to antimicrobial drugs. The study was carried out at La Paz Medical Center, Microbiology section, Malabo, Equatorial Guinea from August 2013 to October 2015. Out of 293 septicemia suspected cases, 29 (10%) blood cultures were positive, 28 with bacterial growth and 1 with growth of Candida sp. The mortality rate of neonates caused by Gram negative bacterial sepsis was 34.7%. Among the Gram negative bacteria (24 isolates), the most common types were Klebsiella pneumoniae (16 = 69.6%), followed by Escherichia coli (4 = 17.4%) and Acinetobacter species (4 = 17.4%). Four Gram positive bacteria were also isolated and identified all ascoagulase-negative staphylococci. All the Klebsiella pneumoniae isolates and Acinetobacter species demonstrated Multi Drug Resistance against different antibiotics with Extended-spectrum β-lactamase (ESBL) activity. The most frequent causative agent of bacterial sepsis in new-born children was Klebsiella pneumoniae. An alarming level of Multi Drug Resistance (MDR) Klebsiella pneumoniae strains to the first choice antibiotic treatment was observed.
文摘Klebsiella pneumoniae(K.pneumoniae)is one of the main gram-negative bacilli in clini- cal practice.Nosocomial infections caused by K.pneumoniae producing extended-spectrumβ-lactama- ses(ESBLs)are very difficult to treat.This paper investigated the resistant characteristics of K.pneu- moniae producing ESBLs and their aminoglycoside-modifying enzyme gene expressions including N- acetyhransferases and O-adenyhransfemses.Bacteria identification and ESBLs confirmatory tests were performed by Phoenix^(TM)-100 system.And minimum inhibitory concentrations(MICs)of gentamicin, amikacin,kanamycin,tobramycin,netilmicin and neomycin in 53 K.pneumoniae isolates were de- tected by agar dilution.In addition,six aminoglycoside-modifying enzyme genes were amplified by polymerase chain reaction(PCR)and verified by DNA sequencer.It was found that imipenem and meropenem against 120 K.pneumoniae isolates produced powerful antimicrobial activities.The resis- tant rates of gentamicin and amikacin were 55.0% and 46.7%,respectively.Except neomycin, MIC_(50)and MIC_(90)of gentamicin,amikacin,kanamycin,tobramycin and netilmicin in 53 K.pneumoni- ae were all>128μg/ml,and the resistant rates were 83.0%,52.3%,75.5%,81.1% and 69.8%,respectively.However,neomycin was only 39.6%.In addition,five modifying enzyme genes,including aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6')-Ⅰb,ant(3″)-Ⅰ,ant(2″)-Ⅰgenes,were found in 53 isoahes except aac(6')-Ⅱ,and their positive rates were 11.3%,67.9%,47.2%, 1.9% and 39.6%,respectively.It was also confirmed by nucleotide sequence analysis that the above resistant genes shared nearly 100% identities with GenBank published genes.The results obtained in the present study indicated that K.pneumoniae producing ESBLs strains are rapidly spreading in our hospital,and their resistance to aminoglycosides may be associated with aminoglycoside-modifying enz- yme gene expressions.
