Histone H3 Lys36(H3K36)methylation and its associated modifiers are crucial for DNA double-strand break(DSB)repair,but the mechanism governing whether and how different H3K36 methylation forms impact repair pathways i...Histone H3 Lys36(H3K36)methylation and its associated modifiers are crucial for DNA double-strand break(DSB)repair,but the mechanism governing whether and how different H3K36 methylation forms impact repair pathways is unclear.Here,we unveil the distinct roles of H3K36 dimethylation(H3K36me2)and H3K36 trimethylation(H3K36me3)in DSB repair via non-homologous end joining(NHEJ)or homologous recombination(HR).Yeast cells lacking H3K36me2 or H3K36me3 exhibit reduced NHEJ or HR efficiency.y Ku70 and Rfa1 bind H3K36me2-or H3K36me3-modified peptides and chromatin,respectively.Disrupting these interactions impairs y Ku70 and Rfa1 recruitment to damaged H3K36me2-or H3K36me3-rich loci,increasing DNA damage sensitivity and decreasing repair efficiency.Conversely,H3K36me2-enriched intergenic regions and H3K36me3-enriched gene bodies independently recruit y Ku70 or Rfa1 under DSB stress.Importantly,human KU70 and RPA1,the homologs of y Ku70 and Rfa1,exclusively associate with H3K36me2 and H3K36me3 in a conserved manner.These findings provide valuable insights into how H3K36me2 and H3K36me3 regulate distinct DSB repair pathways,highlighting H3K36 methylation as a critical element in the choice of DSB repair pathway.展开更多
The broadband, narrow width, rectangular dielectric resonator antenna(RDRA) of aluminum nitride(εr=8.6) was designed and the effect of inclusion of air gap at the bottom of the dielectric resonator antenna(DRA),above...The broadband, narrow width, rectangular dielectric resonator antenna(RDRA) of aluminum nitride(εr=8.6) was designed and the effect of inclusion of air gap at the bottom of the dielectric resonator antenna(DRA),above the ground plane, was investigated. Gain around 7 dBi was obtained for DRA with air gap(DRAAG) over a broad bandwidth in upper X, Ku, and K bands. Further enhancement in gain could be obtained by placing a metal wall parallel to the length of DRA. However, due to the presence of metal wall, bandwidth was reduced. These structures with the metal wall are capable of operating over a wide band extending from Ku band to lower K band with the gain of around 10 dBi. CST Microwave Studio Software was used to simulate all these structures.Performance parameters of DRA with air gap were compared with several broadband DRA structures reported in recent literature. The proposed DRAAG with the metal wall in this paper is capable of operating over a wide bandwidth along with a significant gain.展开更多
基金supported by the National Key Research and Development Program of China(2019YFA0802501)the National Natural Science Foundation of China(32270617,31971231)+1 种基金the Fundamental Research Funds for the Central Universities(2042022dx0003)the Application Fundamental Frontier Foundation of Wuhan(2020020601012225)。
文摘Histone H3 Lys36(H3K36)methylation and its associated modifiers are crucial for DNA double-strand break(DSB)repair,but the mechanism governing whether and how different H3K36 methylation forms impact repair pathways is unclear.Here,we unveil the distinct roles of H3K36 dimethylation(H3K36me2)and H3K36 trimethylation(H3K36me3)in DSB repair via non-homologous end joining(NHEJ)or homologous recombination(HR).Yeast cells lacking H3K36me2 or H3K36me3 exhibit reduced NHEJ or HR efficiency.y Ku70 and Rfa1 bind H3K36me2-or H3K36me3-modified peptides and chromatin,respectively.Disrupting these interactions impairs y Ku70 and Rfa1 recruitment to damaged H3K36me2-or H3K36me3-rich loci,increasing DNA damage sensitivity and decreasing repair efficiency.Conversely,H3K36me2-enriched intergenic regions and H3K36me3-enriched gene bodies independently recruit y Ku70 or Rfa1 under DSB stress.Importantly,human KU70 and RPA1,the homologs of y Ku70 and Rfa1,exclusively associate with H3K36me2 and H3K36me3 in a conserved manner.These findings provide valuable insights into how H3K36me2 and H3K36me3 regulate distinct DSB repair pathways,highlighting H3K36 methylation as a critical element in the choice of DSB repair pathway.
文摘The broadband, narrow width, rectangular dielectric resonator antenna(RDRA) of aluminum nitride(εr=8.6) was designed and the effect of inclusion of air gap at the bottom of the dielectric resonator antenna(DRA),above the ground plane, was investigated. Gain around 7 dBi was obtained for DRA with air gap(DRAAG) over a broad bandwidth in upper X, Ku, and K bands. Further enhancement in gain could be obtained by placing a metal wall parallel to the length of DRA. However, due to the presence of metal wall, bandwidth was reduced. These structures with the metal wall are capable of operating over a wide band extending from Ku band to lower K band with the gain of around 10 dBi. CST Microwave Studio Software was used to simulate all these structures.Performance parameters of DRA with air gap were compared with several broadband DRA structures reported in recent literature. The proposed DRAAG with the metal wall in this paper is capable of operating over a wide bandwidth along with a significant gain.
