目的:探讨响应面法优化苦水玫瑰精油提取后废弃物中总黄酮的分离纯化工艺及抗氧化活性,为回收利用苦水玫瑰精油提取后废弃物中的总黄酮提供依据。方法:通过静态实验研究D101型树脂对苦水玫瑰精油提取后废弃物中黄酮类化合物的吸附和解...目的:探讨响应面法优化苦水玫瑰精油提取后废弃物中总黄酮的分离纯化工艺及抗氧化活性,为回收利用苦水玫瑰精油提取后废弃物中的总黄酮提供依据。方法:通过静态实验研究D101型树脂对苦水玫瑰精油提取后废弃物中黄酮类化合物的吸附和解析能力,之后在单因素动态实验的基础上,采用响应面法优化苦水玫瑰精油提取后废弃物中总黄酮分离纯化工艺参数。在此基础上,以维生素C(VC)为对照,研究纯化后总黄酮对ABTS+·、DPPH·、·OH的清除能力。结果:静态实验吸附量1.83 mg/g,吸附率86.10%,洗脱率88.07%,说明D101型树脂对苦水玫瑰精油提取后废弃物中黄酮类化合物有较好的吸附和解析能力;响应面法优化的动态吸附工艺参数为上样液p H2.12、上样液质量浓度10.4 mg/L、上样液流速1.72 m L/min,吸附率为95.06%;用60 m L无水乙醇溶液洗脱,解析率达到98.30%;分离纯化后的总黄酮纯度可达5.651%;纯化后的总黄酮对ABTS+·、DPPH·、·OH清除作用的IC50分别为:0.03、0.1、8μg/m L,最大清除率分别为:98.96%、89.76%、65.45%,可以看出废弃物中总黄酮具有较好的抗氧化活性。展开更多
Arge huazhongia Wei is a new pest on Kushui Rose in Lanzhou City of China, with two generations a year. The larvae duster together to feed on rose leaves. Female adults lay their eggs at the top of one-year-old branch...Arge huazhongia Wei is a new pest on Kushui Rose in Lanzhou City of China, with two generations a year. The larvae duster together to feed on rose leaves. Female adults lay their eggs at the top of one-year-old branches of Kushui Rose. The mature larvae survive the winter cocooning in the rhizosphere of rose fallen leaves and frass, and emerge into adults in middle or late May. The injury rate of rose plants reaches 81.4%, and that of one-year-old shoots reaches 30.6%.展开更多
文摘目的:探讨响应面法优化苦水玫瑰精油提取后废弃物中总黄酮的分离纯化工艺及抗氧化活性,为回收利用苦水玫瑰精油提取后废弃物中的总黄酮提供依据。方法:通过静态实验研究D101型树脂对苦水玫瑰精油提取后废弃物中黄酮类化合物的吸附和解析能力,之后在单因素动态实验的基础上,采用响应面法优化苦水玫瑰精油提取后废弃物中总黄酮分离纯化工艺参数。在此基础上,以维生素C(VC)为对照,研究纯化后总黄酮对ABTS+·、DPPH·、·OH的清除能力。结果:静态实验吸附量1.83 mg/g,吸附率86.10%,洗脱率88.07%,说明D101型树脂对苦水玫瑰精油提取后废弃物中黄酮类化合物有较好的吸附和解析能力;响应面法优化的动态吸附工艺参数为上样液p H2.12、上样液质量浓度10.4 mg/L、上样液流速1.72 m L/min,吸附率为95.06%;用60 m L无水乙醇溶液洗脱,解析率达到98.30%;分离纯化后的总黄酮纯度可达5.651%;纯化后的总黄酮对ABTS+·、DPPH·、·OH清除作用的IC50分别为:0.03、0.1、8μg/m L,最大清除率分别为:98.96%、89.76%、65.45%,可以看出废弃物中总黄酮具有较好的抗氧化活性。
基金Supported by Agricultural Science and Technology Research Projects of Lanzhou Science and Technology Bureau(2008-1-46)
文摘Arge huazhongia Wei is a new pest on Kushui Rose in Lanzhou City of China, with two generations a year. The larvae duster together to feed on rose leaves. Female adults lay their eggs at the top of one-year-old branches of Kushui Rose. The mature larvae survive the winter cocooning in the rhizosphere of rose fallen leaves and frass, and emerge into adults in middle or late May. The injury rate of rose plants reaches 81.4%, and that of one-year-old shoots reaches 30.6%.
