The Cu2O/TiO2 p-n heterojunction composite photoelectrodes were prepared by depositing Cu 2 O nanoparticles on the surface of TiO 2 nanotubes via anodic oxidation and constant current deposition.Field emission scannin...The Cu2O/TiO2 p-n heterojunction composite photoelectrodes were prepared by depositing Cu 2 O nanoparticles on the surface of TiO 2 nanotubes via anodic oxidation and constant current deposition.Field emission scanning electron microscopy(SEM)and high-resolution transmission electron microscopy(HRTEM)analyses showed that Cu 2 O nanoparticles not only deposited on the surface of TiO 2 nanotube array,but also on the wall of TiO 2 nanotubes.The Cu 2 O deposition amount could be adjusted by changing the deposition time.The photoelectrochemical cathodic protection(PECCP)performance of the prepared photoelectrodes for 316L stainless steel(SS)was tested under visible light.The constant current deposition time had a signifi cant eff ect on the PECCP performance of Cu 2 O/TiO 2-X photoelectrodes and Cu 2 O/TiO 2-20 had the best PECCP performance for the coupled 316L SS.This was attributed to the appropriate amount and thickness of Cu 2 O to form p-n heterojunctions with TiO 2,in which separation of the photogenerated carriers was accelerated and transfer of the photogenerated electrons to 316L SS for PECCP was facilitated.展开更多
Previous studies have shown that vagus nerve stimulation can improve the prognosis of trau- matic brain injury. The aim of this study was to elucidate the mechanism of the neuroprotective effects of vagus nerve stimul...Previous studies have shown that vagus nerve stimulation can improve the prognosis of trau- matic brain injury. The aim of this study was to elucidate the mechanism of the neuroprotective effects of vagus nerve stimulation in rabbits with brain explosive injury. Rabbits with brain ex- plosive injury received continuous stimulation (10 V, 5 Hz, 5 ms, 20 minutes) of the right cervical vagus nerve. Tumor necrosis factor-a, interleukin-l~ and interleukin-10 concentrations were detected in serum and brain tissues, and water content in brain tissues was measured. Results showed that vagus nerve stimulation could reduce the degree of brain edema, decrease tumor necrosis factor-a and interleukin-1β concentrations, and increase interleukin-10 concentration after brain explosive injury in rabbits. These data suggest that vagus nerve stimulation may exert neuroprotective effects against explosive injury via regulating the expression of tumor necrosis factor-a, interleukin-1 β and interleukin-10 in the serum and brain tissue.展开更多
[Objective] This study aimed to investigate the effects of space mutation on the activities of protective enzymes in different strains of Cichorium intybus L. and provide theoretical basis for screening drought-tolera...[Objective] This study aimed to investigate the effects of space mutation on the activities of protective enzymes in different strains of Cichorium intybus L. and provide theoretical basis for screening drought-tolerant plants in southern karst area. [Method] C. intybus seeds were sent into outer space by 'Shijian No.8' seed- breeding satellite. The new strains and original variety of C. intybus L. were used as experimental materials to determine the activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and malondialdehyde (MDA) content. [Result] The results showed that MDA content in PA-82, Puna chicory and PA-43 increased gradually with the increase of water stress duration, which was enhanced by 0.575, 0.72 and 0.844 μmol/g respectively at 12 days. In addition, the activities of SOD, POD and CAT increased rapidly; at 9 days, the activities of SOD in PA-82, Puna chicory and PA-43 were enhanced by 878.9, 809.2 and 711.1 U/g, with the increas- ing range of 139.1%, 136.7% and 121.1%, respectively; the activities of POD were enhanced by 4 397, 3 754 and 2 767 U/(g ·min), with the increasing range of 265.84%, 257.65% and 204.06%, respectively; the activities of CAT were enhanced by 402.1, 277.1 and 170.9 KU/L, with the increasing range of 73.08%, 39.69% and 26.20%, respectively; subsequently, the activities of these three protective enzymes were reduced significantly; at 12 days, the activities of SOD in PA-82, Puna chicory and PA-43 were reduced to 1206.5, 1144.7 and 1108.6 U/g, respectively; the activi- ties of POD were reduced to 3 145, 2 876 and 2 753 U/(g·min) , respectively; the activities of CAT were reduced to 587.2, 698.1 and 584.1 KU/L, respectively. [Con- clusion] According to the results of comprehensive evaluation, drought resistance in three C. intybus varieties exhibited a descending order of PA-82 〉 Puna chicory 〉 PA-43.展开更多
The experiment was conducted to investigate the effect of transfer of PUFA protected and carnitin precursor on the ration of chemical composition of milk dairy goat. In total, 10 female dairy goats of 2 - 4 years old ...The experiment was conducted to investigate the effect of transfer of PUFA protected and carnitin precursor on the ration of chemical composition of milk dairy goat. In total, 10 female dairy goats of 2 - 4 years old Peranakan Etawah (PE) with body weight of 25 - 45 kg were used in this experiment. The feed material included a basal diet (control) based on yellow corn, rice bran, soya bean meal, coconut meal, tuna fish oil and lemuru fish oil. The method of the research was experimental in vivo using Randomized Completely Block Design (RCBD). There were 5 treatments in each experiment and 2 replications. The treatment consisted of P0 = control ration, P1 = P0 + 200 ppm L-carnitine on the ration, P2 = P1 + soya bean oil, P3 = P1 + protected tuna fish oil 5 ml or equal with 4% in the ration, and P4 = P1 + protected lemuru fish oil 5 ml or equal with 4% in the ration. The measured variable is chemical composition of milk dairy goat. The results of variance analysis showed that the effect of transfer of PUFA fatty acid in the rations contained 200 ppm L-carnitine significantly展开更多
<span style="font-family:Verdana;"><i><span style="font-family:Verdana;"><i></span></i></span><span style="font-family:Verdana;"><span s...<span style="font-family:Verdana;"><i><span style="font-family:Verdana;"><i></span></i></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">S</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">-allyl-L-cysteine (SAC) is an organosulfur compound derived from aged garlic extract (</span><span style="font-family:Verdana;">AGE</span><span style="font-family:Verdana;">). Studies have reported that AGE possesses bioprotective capacity, including antidiabetic, antimicrobial, antioxidant, and antitumor effects. The present study examined the protective effects of SAC against carbon tetrachloride (CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">) induced hepatotoxicity in rats. Ten male Wistar rats aged 11 - 12 weeks were randomly divided into two groups (five rats/group) as control and SAC groups. All rats had </span><span style="font-family:Verdana;">ad</span> <span style="font-family:Verdana;">libitum</span><span style="font-family:Verdana;"> access to water, and the SAC group received water containing SAC intragastrically (200 mg/kg) once daily for five consecutive weeks. In the fifth experimental week, 50% CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> in olive oil (1 mL/kg) was administered intraperitoneally three times a week to induce liver injury in both groups. Rats were sacrificed at 24 hours after the last CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> injection, and liver tissues were excised for histopathological, immunohistochemical and antioxidant analyses. The rats in the SAC group did not show abnormal behavior, such as decreased water intake or food consumption, during the experimental period. Body weights in all groups did not change significantly over the experimental period. Histopathological analysis showed that the percentage of hepatic steatosis was lower in the SAC group at 12.75% ± 3.74% compared to 24.64% ± 5.29% in the control group (</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> < 0.05). The percentage of cytochrome P4502E1 (CYP2E1) distribution area in the SAC group was also lower at 19.61% ± 6.18% compared with 25.22% ± 6.21% in the control group (</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> < 0.05). These results suggest that SAC can alleviate CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">-induced liver damage by decreasing hepatic steatosis and reducing CYP2E1 expression in rats.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.41576114,41676069)the State Key Laboratory for Marine Corrosion and Protection,Luoyang Ship Material Research Institute,China(No.614290101011703)the Qingdao Innovative Leading Talent Foundation(No.15-10-3-15-(39)-zch)。
文摘The Cu2O/TiO2 p-n heterojunction composite photoelectrodes were prepared by depositing Cu 2 O nanoparticles on the surface of TiO 2 nanotubes via anodic oxidation and constant current deposition.Field emission scanning electron microscopy(SEM)and high-resolution transmission electron microscopy(HRTEM)analyses showed that Cu 2 O nanoparticles not only deposited on the surface of TiO 2 nanotube array,but also on the wall of TiO 2 nanotubes.The Cu 2 O deposition amount could be adjusted by changing the deposition time.The photoelectrochemical cathodic protection(PECCP)performance of the prepared photoelectrodes for 316L stainless steel(SS)was tested under visible light.The constant current deposition time had a signifi cant eff ect on the PECCP performance of Cu 2 O/TiO 2-X photoelectrodes and Cu 2 O/TiO 2-20 had the best PECCP performance for the coupled 316L SS.This was attributed to the appropriate amount and thickness of Cu 2 O to form p-n heterojunctions with TiO 2,in which separation of the photogenerated carriers was accelerated and transfer of the photogenerated electrons to 316L SS for PECCP was facilitated.
文摘Previous studies have shown that vagus nerve stimulation can improve the prognosis of trau- matic brain injury. The aim of this study was to elucidate the mechanism of the neuroprotective effects of vagus nerve stimulation in rabbits with brain explosive injury. Rabbits with brain ex- plosive injury received continuous stimulation (10 V, 5 Hz, 5 ms, 20 minutes) of the right cervical vagus nerve. Tumor necrosis factor-a, interleukin-l~ and interleukin-10 concentrations were detected in serum and brain tissues, and water content in brain tissues was measured. Results showed that vagus nerve stimulation could reduce the degree of brain edema, decrease tumor necrosis factor-a and interleukin-1β concentrations, and increase interleukin-10 concentration after brain explosive injury in rabbits. These data suggest that vagus nerve stimulation may exert neuroprotective effects against explosive injury via regulating the expression of tumor necrosis factor-a, interleukin-1 β and interleukin-10 in the serum and brain tissue.
基金Supported by Science and Technology Fund of Guizhou Province(QKHJZ[2011]2165)Key Project of Agricultural Science and Technology of Guizhou Province(QKHNYZ[2009]3083,QKHNYZ[2012]3049)Special Breeding Fund of Guizhou Province(QNYZZ[2011]036)~~
文摘[Objective] This study aimed to investigate the effects of space mutation on the activities of protective enzymes in different strains of Cichorium intybus L. and provide theoretical basis for screening drought-tolerant plants in southern karst area. [Method] C. intybus seeds were sent into outer space by 'Shijian No.8' seed- breeding satellite. The new strains and original variety of C. intybus L. were used as experimental materials to determine the activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and malondialdehyde (MDA) content. [Result] The results showed that MDA content in PA-82, Puna chicory and PA-43 increased gradually with the increase of water stress duration, which was enhanced by 0.575, 0.72 and 0.844 μmol/g respectively at 12 days. In addition, the activities of SOD, POD and CAT increased rapidly; at 9 days, the activities of SOD in PA-82, Puna chicory and PA-43 were enhanced by 878.9, 809.2 and 711.1 U/g, with the increas- ing range of 139.1%, 136.7% and 121.1%, respectively; the activities of POD were enhanced by 4 397, 3 754 and 2 767 U/(g ·min), with the increasing range of 265.84%, 257.65% and 204.06%, respectively; the activities of CAT were enhanced by 402.1, 277.1 and 170.9 KU/L, with the increasing range of 73.08%, 39.69% and 26.20%, respectively; subsequently, the activities of these three protective enzymes were reduced significantly; at 12 days, the activities of SOD in PA-82, Puna chicory and PA-43 were reduced to 1206.5, 1144.7 and 1108.6 U/g, respectively; the activi- ties of POD were reduced to 3 145, 2 876 and 2 753 U/(g·min) , respectively; the activities of CAT were reduced to 587.2, 698.1 and 584.1 KU/L, respectively. [Con- clusion] According to the results of comprehensive evaluation, drought resistance in three C. intybus varieties exhibited a descending order of PA-82 〉 Puna chicory 〉 PA-43.
文摘The experiment was conducted to investigate the effect of transfer of PUFA protected and carnitin precursor on the ration of chemical composition of milk dairy goat. In total, 10 female dairy goats of 2 - 4 years old Peranakan Etawah (PE) with body weight of 25 - 45 kg were used in this experiment. The feed material included a basal diet (control) based on yellow corn, rice bran, soya bean meal, coconut meal, tuna fish oil and lemuru fish oil. The method of the research was experimental in vivo using Randomized Completely Block Design (RCBD). There were 5 treatments in each experiment and 2 replications. The treatment consisted of P0 = control ration, P1 = P0 + 200 ppm L-carnitine on the ration, P2 = P1 + soya bean oil, P3 = P1 + protected tuna fish oil 5 ml or equal with 4% in the ration, and P4 = P1 + protected lemuru fish oil 5 ml or equal with 4% in the ration. The measured variable is chemical composition of milk dairy goat. The results of variance analysis showed that the effect of transfer of PUFA fatty acid in the rations contained 200 ppm L-carnitine significantly
文摘<span style="font-family:Verdana;"><i><span style="font-family:Verdana;"><i></span></i></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">S</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">-allyl-L-cysteine (SAC) is an organosulfur compound derived from aged garlic extract (</span><span style="font-family:Verdana;">AGE</span><span style="font-family:Verdana;">). Studies have reported that AGE possesses bioprotective capacity, including antidiabetic, antimicrobial, antioxidant, and antitumor effects. The present study examined the protective effects of SAC against carbon tetrachloride (CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">) induced hepatotoxicity in rats. Ten male Wistar rats aged 11 - 12 weeks were randomly divided into two groups (five rats/group) as control and SAC groups. All rats had </span><span style="font-family:Verdana;">ad</span> <span style="font-family:Verdana;">libitum</span><span style="font-family:Verdana;"> access to water, and the SAC group received water containing SAC intragastrically (200 mg/kg) once daily for five consecutive weeks. In the fifth experimental week, 50% CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> in olive oil (1 mL/kg) was administered intraperitoneally three times a week to induce liver injury in both groups. Rats were sacrificed at 24 hours after the last CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> injection, and liver tissues were excised for histopathological, immunohistochemical and antioxidant analyses. The rats in the SAC group did not show abnormal behavior, such as decreased water intake or food consumption, during the experimental period. Body weights in all groups did not change significantly over the experimental period. Histopathological analysis showed that the percentage of hepatic steatosis was lower in the SAC group at 12.75% ± 3.74% compared to 24.64% ± 5.29% in the control group (</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> < 0.05). The percentage of cytochrome P4502E1 (CYP2E1) distribution area in the SAC group was also lower at 19.61% ± 6.18% compared with 25.22% ± 6.21% in the control group (</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;">p</span></i></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><i><span style="font-family:Verdana;"></i></span></i></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> < 0.05). These results suggest that SAC can alleviate CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">-induced liver damage by decreasing hepatic steatosis and reducing CYP2E1 expression in rats.