We introduce the generalized area operators by using nonnegative measures defined on upper half-spaces R+^n+1. The characterization of the boundedness and compactness of the generalized area operator from LP(]Rn) ...We introduce the generalized area operators by using nonnegative measures defined on upper half-spaces R+^n+1. The characterization of the boundedness and compactness of the generalized area operator from LP(]Rn) to Lq(IRn) is investigated in terms of s-Carleson measures with 1 〈 p, q 〈 +∞. In the case of p = q = 1, the weak type estimate is also obtained.展开更多
目的:构建大鼠线粒体转录因子TFAM(A)、线粒体转录因子TFB1M(B1)、线粒体转录TFB2M(B2)及线粒体RNA聚合酶(POLRMT)的质粒标准品,为检测内耳细胞线粒体TFAM、TFB1M、TFB2M、POLRMT的mRNA表达水平做基础。方法:设计特异性引物和探针,提取...目的:构建大鼠线粒体转录因子TFAM(A)、线粒体转录因子TFB1M(B1)、线粒体转录TFB2M(B2)及线粒体RNA聚合酶(POLRMT)的质粒标准品,为检测内耳细胞线粒体TFAM、TFB1M、TFB2M、POLRMT的mRNA表达水平做基础。方法:设计特异性引物和探针,提取大鼠内耳组织总mRNA逆转录成c DNA,PCR扩增、纯化目的片段,将纯化产物与p Zero Back/blunt载体重组,提取重组质粒,经测序鉴定后,用实时荧光绝对定量PCR建立标准曲线。结果:测序结果与各目的序列一致,获得良好的标准曲线(R2>0.99)。结论:成功构建了各目的基因的质粒标准品。展开更多
基金Acknowledgements The authors were partially supported by the National Natural Science Foundation of China (Grant No. 11271162), the Natural Science Foundation of Zhejiang Province (Y6110824), and the second author was also partially supported by the Natural Science Foundation of Zhejiang Province (Y6100810).
文摘We introduce the generalized area operators by using nonnegative measures defined on upper half-spaces R+^n+1. The characterization of the boundedness and compactness of the generalized area operator from LP(]Rn) to Lq(IRn) is investigated in terms of s-Carleson measures with 1 〈 p, q 〈 +∞. In the case of p = q = 1, the weak type estimate is also obtained.
基金Supported by the National Natural Science Foundation of China(11871452,12071052the Natural Science Foundation of Henan(202300410338)the Nanhu Scholar Program for Young Scholars of XYNU。
文摘目的:构建大鼠线粒体转录因子TFAM(A)、线粒体转录因子TFB1M(B1)、线粒体转录TFB2M(B2)及线粒体RNA聚合酶(POLRMT)的质粒标准品,为检测内耳细胞线粒体TFAM、TFB1M、TFB2M、POLRMT的mRNA表达水平做基础。方法:设计特异性引物和探针,提取大鼠内耳组织总mRNA逆转录成c DNA,PCR扩增、纯化目的片段,将纯化产物与p Zero Back/blunt载体重组,提取重组质粒,经测序鉴定后,用实时荧光绝对定量PCR建立标准曲线。结果:测序结果与各目的序列一致,获得良好的标准曲线(R2>0.99)。结论:成功构建了各目的基因的质粒标准品。