Breeding of a Natural Colored-Cocoon Bombyx mori Variety Shuhuang No.1

[Objective] This study aimed to develop a new Bombyx mori variety Shuhuang No.1 and investigate its characteristics.[Method] The new variety was developed through cross breeding and pedigree separation.Its characteristics were identified by laboratory tests and rural promotion practices in Sichuan Province.[Result]The cocoon produced by the new variety Shuhuang No.1 was golden-yellow and radiantly beautiful.Shuhuang No.1 was strong and easy to rear,uniform in development.The male and female ones could be distinguished by their larval color and markings.The cocoon yield per 10 000 the 4th instar larvae reached 20.30 kg; the length of a cocoon filament was about 1 115.0 m,and a non-broken filament was 882.95 mm long,accounting for 79.15％ of the total length.The raw silk ratio of fresh cocoon was 18.39％,and the neatness was 91.25 point.The main economic features of Shuhuang No.1 were better than those of the control.[Conclusion] The new variety had been approved by Sichuan Silkworm Evaluation Commission.It can be promoted in parent silkworm rearing areas Sichuan,Chongqing,Yangtze River basin.

Study on SSR Markers Linked to Flesh Color around the Stone of Prunus persica(L.) Batsch

[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.

迈腾B8L发动机故障诊断与修复

发动机电控系统诊断与修复是汽车技术国赛考核的重点,也是教学的难点。笔者结合自身参赛经历和发动机电控系统诊断与修复的教学经验,总结迈腾B8L1.4T发动机故障诊断与排除流程,对于教师教学备课和技能竞赛备赛具有参考意义。

采用color—pilot颜色校正软件校正比色片数码照片并分析其在CIEL＊a＊b＊色度空间中的位置变化

目的:采用color-pilot颜色校正软件校正比色片数码照片并分析其在色度空间中的位置变化。方法:采用Vita 3D Master比色板作为试件,在诊室内拍摄比色片数码照片,并运用color-pilot颜色校正软件进行校正,最后与暗箱内拍摄的相应比色片数码照片进行对比。结果:比较诊室内与暗箱内拍摄的比色片数码照片,发现两组照片L*值之间、a*值之间差异明显(P<0.05),b*值之间则无明显差异(P>0.05);运用color-pilot颜色校正软件校正诊室内拍摄的数码照片后,发现两组照片L*值之间的差异仍然存在(P<0.05),但差异缩小(较诊室内拍摄的数码照片,校正后的数码照片L*值更接近于暗箱内拍摄的数码照片L*值),而a*值之间无明显差异(P>0.05),b*值之间依然无明显差异(P>0.05)。二者在色度空间中的位置也较校正前更为靠近。计算暗箱内、诊室内、校正后3组照片的ΔE值发现,校正后与暗箱内数码照片此值最小。结论:color-pilot颜色校正软件校正比色片数码照片后,可以在一定程度上减小数码照片的色彩偏差。

非洲猪瘟病毒B602L蛋白的原核表达、纯化及多克隆抗体制备

【目的】非洲猪瘟病毒(African swine fever virus,ASFV)是对生猪养殖业危害极大的一种病原体,B602L蛋白作为其重要的非结构蛋白,是衣壳蛋白p72折叠为正确的三级结构的伴侣分子之一,在病毒组装成二十面体结构的过程中发挥着关键作用。特异性抗体对于抗非洲猪瘟免疫应答机制及血清学检测方法研究具有重要意义,本研究旨在获取ASFV B602L蛋白,并制备抗ASFV B602L蛋白的多克隆抗体,为ASFV B602L蛋白功能研究及相关诊断试剂的研发提供材料。【方法】以ASFV China/2018/AnhuiXCGQ株的B 602 L基因为研究对象,应用生物信息学软件对B602L蛋白的理化性质、信号肽、跨膜结构、抗原性和蛋白结构进行分析后,利用同源重组的方法将其克隆至原核表达载体pET-28a(+)中,转化大肠杆菌BL21(DE3)感受态细胞后经诱导表达获得B602L重组蛋白,利用镍柱亲和层析进行蛋白纯化,通过SDS-PAGE和Western blotting对B602L蛋白纯化效果进行验证。将纯化后的B602L蛋白免疫BALB/c小鼠制备多克隆抗体,通过间接ELISA方法测定多克隆抗体效价,通过Western blotting和Dot blotting检测多克隆抗体特异性。【结果】生物信息学分析表明,B602L蛋白属于稳定性亲水蛋白,无跨膜区和信号肽。二级结构主要含有α-螺旋(54.91%)、延伸链(9.81%)和无规则卷曲(32.08%)。成功构建了ASFV B602L重组质粒,SDS-PAGE和Western blotting结果显示,重组菌pET-28a-B602L-BL21在16℃、0.5 mmol/L IPTG诱导下高效表达,获得的重组蛋白主要表达在BL21菌株上清中,大小约为70 ku,且能与ASFV阳性血清特异性结合。间接ELISA检测结果显示,制备的抗B602L鼠源多克隆抗体效价达1∶409600,Western blotting和Dot blotting试验结果表明,抗B602L鼠源多克隆抗体能够特异性识别B602L蛋白。【结论】原核表达的B602L重组蛋白具有良好的免疫原性,能够与ASFV阳性血清特异性结合,制备的鼠抗ASFV B602L蛋白多克隆抗体具备较好的生物学活性。本试验结果可为进一步研究ASFV B602L蛋白的生物学功能和非洲猪瘟诊断方法的建立提供生物材料和理论支撑。

MyD88L265P及CD79B基因突变对原发性中枢神经系统淋巴瘤预后的影响

目的探究MyD88L265P及CD79B基因突变对原发性中枢神经系统淋巴瘤(PCNSL)患者预后的影响。方法收集2018年8月-2020年11月于兰州大学第二医院经开颅手术或立体定位活检术确诊的18例免疫功能正常(无HIV感染及免疫抑制药服用病史)的PCNSL患者临床资料进行回顾性分析。分别采用实时荧光定量PCR和一代基因测序技术检测18例PCNSL患者肿瘤组织中MyD88L265P和CD79B基因突变情况。针对可能与PCNSL首次无疾病进展生存期(PFS)和总生存期相关的指标行单因素分析,并采用Cox回归进行多因素分析。结果18例PCNSL肿瘤组织MyD88L265P突变率为38.9%,CD79B突变率为33.3%,MyD88L265P/CD79B共突变率为27.8%。单因素分析结果显示,病灶多发、病灶深部受累、肿瘤组织CD79B突变患者的PFS时间更短(P<0.05);多因素分析结果显示,病灶深部受累(HR=0.135,95%CI 0.023~0.799,P<0.05)、肿瘤组织CD79B突变(HR=0.149,95%CI 0.028~0.800,P<0.05)是PCNSL患者预后不良的独立危险因素。结论本组PCNSL患者肿瘤组织MyD88L265P、CD79B突变频率较高,这两种基因突变尤其是CD79B突变可能与PCNSL不良预后相关。

汽油车无法发动综合故障快速诊断研究分析——以大众迈腾B8L为例

本文主要分析汽油发动机作为动力主体的燃油车辆无法发动的具体故障现象以及根据故障现象快速判断故障方向的方法。详细阐述以大众迈腾B8L为例的汽油车无法发动的故障现象与原因以及测量方法,总结了汽油车无法发动故障的快速诊断诀窍。内容包含汽油发动机电控系统的全部内容与要点,汽油车仍旧是社会交通工具的主体,本文仍具有一定的研究价值与意义。

Evaluation of the Potential of Araucaria angustifolia Seeds as Source of Oligosaccharides, Resistant Starch and Growth of Probiotic Bacteria

“Pinhão”, the seed of Araucaria angustifolia, is an important food, being part of the eating habits of Indigenous communities. In this study, we evaluated the oligosaccharide content, resistant starch and the growth of probiotic bacteria. GF4 (1-fructofuranosylnystose) was the main fructo-oligosaccharides found, in higher contents compared to other food sources. Maltooligosaccharides (MOS) represented the main part of the oligosaccharides profile of Brazilian pine seeds. In descending order of importance was maltoheptaose (G7), maltohexose (G6) and maltotriose (G3). The starches from the variety Sanct josephi presented the highest amount of resistant starch that could stimulate probiotic strains, mainly B. breve and L. plantarum, and may have a prebiotic effect, potentially promoting health benefits. This study advances the understanding of the chemical composition of the main portion of the “pinhão” enhancing awareness of its potential as a healthy food source, contributing to different uses and indirectly with the species preservation.

大众迈腾B8L轿车启动系统工作过程与故障诊断分析

以大众迈腾B8L轿车发动机启动系统为例,分析启动系统的工作过程以及当启动系统出现故障时的诊断流程和思路。分析认为,维修人员熟悉该车发动机启动系统的工作过程,能够对启动系统工作条件是否正常进行判断,以及可以熟练掌握该车发动机启动系统的诊断流程。

Rhizobium Inoculation and Micronutrient Addition Influence the Growth,Yield,Quality and Nutrient Uptake of Garden Peas(Pisum sativum L.)

Garden pea productivity and qualities are hampered in zinc(Zn),boron(B),and molybdenum(Mo)deficient soil.Thus,the combination of micronutrients(i.e.,Zn,B,and Mo)and rhizobium is necessary to increase the productivity and quality of garden peas,since this management for garden peas is neglected in Bangladesh.Therefore,the present study was made to assess the effectiveness of rhizobium inoculant singly or in combination with the micronutrients(i.e.,Zn,B,and Mo)on growth,yield,nutrient uptake,and quality of garden peas.Treatments were:T_(1)=Control,T_(2)=Rhizobium inoculation at 50 g/kg seed,T_(3)=T_(2)+Zn_(3)Mo1,T_(4)=T_(2)+B_(2)Mo1,T_(5)=T_(2)+Zn_(3)B_(2),T_(6)=T_(2)+Zn_(3)B_(2)Mo1 and T_(7)=Zn_(3)B_(2)Mo1.All treatments were arranged in a randomized complete block design and repeated all treatments in three times.The application of 3 kg Zn,2 kg B,and 1 kg Mo ha^(−1)with inoculation of Rhizobium at 50 g kg^(−1)seed(T_(6))facilitated to increase of 44.8%in the green pod and 29.7%seed yield over control.The same treatment contributed to attaining the maximum nodulation(25.3 plant^(−1)),Vitamin C(43.5 mg 100 g^(−1)),protein content(22.2%),and nutrient uptake as well as accumulation in garden peas.Among all treatment combinations,treatment T_(6)was found superior to others based on microbial activities,soil fertility,and profitability.The results of the study found that the application of 3 kg Zn,2 kg B,and 1 kg Mo ha^(−1)in combination with Rhizobium inoculation(50 g kg^(−1)seed)can improve the yield and quality of garden peas.The results of the study have the potential for the areas,where there is no use of Rhizobium inoculant or Zn,B,and Mo fertilizer for cultivation of garden pea.

迈腾B8L发动机无法启动故障诊断与排除

以高职国赛“汽车故障检修”选用的迈腾B8L为对象,诊断与排除电气系统的典型部件损坏造成发动机无法启动的故障。首先,描述了发动机无法启动的故障现象:全车不上电、起动机无法运转及起动机运转不着车等;其次,介绍迈腾B8L的启动策略:整车上电策略及起动机运转策略;再次,选取点火开关损坏造成的不上电故障,动力总线损坏造成的起动机不运转故障,起动继电器及其线路损坏造成的起动机不运转故障,曲轴位置传感器及其线路损坏造成的不着车故障;最后,对点火开关、动力总线、启动继电器及其线路、曲轴位置传感器及其线路的故障进行了诊断与排除。在不使用故障诊断仪的前提下,为以上四大部件损坏造成的发动机无法启动故障,提供了系统、全面、有效的排除方法,也为诊断与排除其他部件的故障提供了详细的参考依据。

A Study on the Influence of Luminance L* in the L*a*b* Color Space during Color Segmentation

In this paper an evaluation of the influence of luminance L* at the L*a*b* color space during color segmentation is presented. A comparative study is made between the behavior of segmentation in color images using only the Euclidean metric of a* and b* and an adaptive color similarity function defined as a product of Gaussian functions in a modified HSI color space. For the evaluation synthetic images were particularly designed to accurately assess the performance of the color segmentation. The testing system can be used either to explore the behavior of a similarity function (or metric) in different color spaces or to explore different metrics (or similarity functions) in the same color space. From the results is obtained that the color parameters a* and b* are not independent of the luminance parameter L* as one might initially assume.

Stochastic modeling and analysis of hepatitis and tuberculosis co-infection dynamics

Several mathematical models have been developed to investigate the dynamics of tuberculosis(TB)and hepatitis B virus(HBV).Numerous current models for TB,HBV,and their co-dynamics fall short in capturing the important and practical aspect of unpredictability.It is crucial to take into account a stochastic co-infection HBV–TB epidemic model since different random elements have a substantial impact on the overall dynamics of these diseases.We provide a novel stochastic co-model for TB and HBV in this study,and we establish criteria on the uniqueness and existence of a nonnegative global solution.We also looked at the persistence of the infections as long its dynamics are governable by the proposed model.To verify the theoretical conclusions,numerical simulations are presented keeping in view the associated analytical results.The infections are found to finally die out and go extinct with certainty when L´evy intensities surpass the specified thresholds and the related stochastic thresholds fall below unity.The findings also demonstrate the impact of noise on the decline in the co-circulation of HBV and TB in a given population.Our results provide insights into effective intervention strategies,ultimately aiming to improve the management and control of TB and HBV co-infections.

Bioactive chemical constituents from the marine-derived fungus Cladosporium sp.DLT-5

A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2–16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidation determined the planar structure of 1.Subsequent electronic circular dichroism(ECD)experiment assigned the absolute configuration of 1.Compounds 1,2,4–6,and 10 displayed different degrees of neuroprotective activities on human neuroblastoma cells SH-SY5Y.Five compounds(1,3–5,and 13)emerged resistance to protein tyrosine phosphatase 1B(PTP1B),further kinetic analysis and molecular docking study indicated that the most potent compound 13(IC50value of 10.74±0.61μmol/L)was found as a noncompetitive inhibitor for PTP1B.Surface plasmon resonance(SPR)and molecular docking studies also demonstrated the interaction between compound 12 and Niemann-Pick C1 Like 1(NPC1L1),which has been identified as significant therapeutic target for hypercholesteremia.In addition,compounds 3,6,and 14 showed attractive inhibitory activity against the phytopathogenic fungi:Colletotrichum capsici.Therefore,library of Cladosporium metabolites is enriched and new active uses of known compounds are explored.

马头山羊羊口疮病毒的分离鉴定及其B2L基因的特征分析

为确定湖北省某马头山羊养殖场发生的疑似羊口疮(Orf)的病原及其分子生物学特征。本试验利用牛睾丸细胞对马头山羊唇部痂皮病料进行病毒分离培养,通过细胞病变观察、病毒滴度测定、PCR扩增和测序等方法对分离毒株进行了鉴定。并采用MegAlign、MEGA 7.0、DNAMAN和Bioedit等软件分析了分离毒株和China Vaccine毒株B2L基因的核苷酸序列相似性,并预测了蛋白二级结构和疏水性。结果显示,接种病料上清液的牛睾丸细胞盲传5代后出现细胞病变,经PCR扩增的分离毒株B2L基因大小为1137 bp;所分离羊口疮病毒(ORFV)病毒滴度可达105.5TCID50/mL;分离毒株与AH1404、FJ-YX和GX-BH毒株B2L基因同源性为99.3%;与China Vaccine毒株同源性为89.5%。分离毒株与China Vaccine毒株B2L基因序列分析显示处在不同分支,两者存在8个位点突变(aa11、aa80、aa101、aa123、aa126、aa294、aa327和aa333);B2L蛋白为稳定的疏水性蛋白;分离毒株和China Vaccine毒株的蛋白二级结构存在差异。结果表明,该养殖场ORFV的B2L基因出现变异,有关部门应当加强对ORFV流行情况的监测。

Crocus sativus L.produces anti-inflammatory effects and regulates the NLRP3–NF-κB pathway

Objective:This study aimed to evaluate the anti-inflammatory effects of petal and stamen extracts of saffron crocus(Crocus sativus)and explore the underlying mechanism.Methods:Local and systemic inflammation models were used to investigate the anti-inflammatory effects of C.sativus.A xyleneinduced inflammation model or lipopolysaccharide(LPS)-induced inflammation model was used in this study.C.sativus petal and stamen extracts were each administered to the mice in the xylene and LPS models by gavage for 14 d at 0.1 and 0.4 g/kg doses,respectively.Enzyme-linked immunosorbent assay(ELISA)was used to measure the concentrations of tumor necrosis factor(TNF)-αand interleukin(IL)-1βin mouse serum.Hematoxylin and eosin(H&E)staining was used to observe the pathological changes in the ear in the xylene-induced inflammation model and in the spleen in the LPS-induced inflammation model.NOD-like receptor thermal protein domain associated protein 3(NLRP3)protein levels within the nuclear factor-kappa B(NF-κB)pathway were assessed using western blotting.RAW264.7 cells were treated with LPS(5μg/mL)and LPS+C.sativus(0.05,0.1,and 0.2 mg/mL)for 24 h,and a Cell Counting Kit-8 was used to measure cell proliferation.Changes in NLRP3 and NF-κB levels were evaluated by western blotting.Results:Petal and stamen extracts of C.sativus attenuated the anti-inflammatory effects in local or systemic inflammatory models and repaired pathological changes in the ear in the xylene-induced inflammation model and spleen in the LPS-induced inflammation model.These extracts also decreased the concentrations of TNF-αand IL-1βin the mouse serum in the LPS-induced inflammation model.C.sativus downregulated NLRP3 protein level through the NF-κB pathway and downregulated LC-3 and BECLIN1 in vivo and in vitro.Carbonyl Cyanide3-ChloroPhenylhydrazone(CCCP)weakened the effects of C.sativus on the NLRP3–NF-κB pathway.Conclusion:C.sativus has anti-inflammatory effects and regulates the NLRP3-NF-κB pathway.

2020款迈腾B8L发动机无法启动故障检修

一辆2020款迈腾B8L轿车,按启动按钮,仪表正常点亮;踩刹车同时按启动按钮,启动机有吸合声音但无法转动,无着车迹象。针对该故障现象,根据故障产生机理,依次对该车发动机启动系统的相关部件及其电路等进行了检测诊断。最终排查出的故障原因是,发动机控制单元J623的P/N挡信号线与启动反馈信号线短路,对其进行修复后,发动机可正常启动,车辆故障排除。

The Color Calibration across Multi-Projector Display

Large plane displays are often achieved by combining tiled imaging elements, which give the appearance of a single, seamless display. However, this is difficult to realize in practical use, since differences in color will make the tiles dis-tinct even if the geometric calibration is perfect. The paper presents an approach of color calibration using cubic B- spline. According to the theory of cubic B-spline, we construct the color transfer function of each projector. This ap-proach reduces the color and photometric non-uniformities to achieve seamless tiled multi-projector display well. Finally, we propose the difference between blending region and overlapping region which can help to achieve a better effect of alpha blending.

弥漫大B细胞淋巴瘤中MYD88、NAP1L5基因特征与临床特征及预后的关系

目的 探讨弥漫性大B细胞淋巴瘤(Diffuse Large B-cell Lymphoma, DLBCL)中MYD88和NAP1L5基因的特征,及其与临床表现和预后之间的关系。方法 分析本院2019年1月至2022年12月期间的87例DLBCL患者中MYD88和NAP1L5基因突变的情况,并分析其与临床特征的关系。评估影响DLBCL患者总生存期的相关因素。结果 87例患者中,31例出现MYD88基因异常,35例出现NAP1L5基因异常。两个基因共异常率为26.43%。MYD88和NAP1L5基因异常及共异常主要集中在ABC型DLBCL中,阳性率分别达74.19%、74.28%、73.91%。在Ⅲ~Ⅳ期病例中,MYD88基因异常和两基因共异常的比例(61.29%、65.21%)均高于I~II期的病例(P<0.05)。在IPI指数为3~5的病例中,MYD88基因异常的比例(64.51%)高于IPI指数为0~2的病例(P<0.05)。生存时间的单因素分析显示,病理分型、临床分期、MYD88、NAP1L5基因异常组和两基因共异常是影响因素。生存分析显示,不同病理分型、临床分期、MYD88基因状态、NAP1L5基因状态、MYD88、NAP1L5基因共异常情况患者的生存时间差异显著(P<0.05)。临床分期、MYD88和NAP1L5基因共异常是影响患者总生存期的独立因素(P<0.05)。结论 MYD88、NAP1L5基因异常与DLBCL的临床特征密切相关,临床分期和MYD88和NAP1L5基因的共异常是影响DLBCL患者总生存期的独立因素。

The color reflectance of marine-terrigenous deposits in LZ908 borehole in south coastal plain of the Laizhou Bay

Color measuring is characterized by high resolution,high efficiency,and low cost. The application becomes increasingly common for tracing sediment sources and climate variation. Borehole LZ908 in the south coastal plain of the Laizhou Bay in northeast China,was used,of which the top 54 m containing all marine facies was focused,to test the feasibility of colorimetry as a climate indi-cator using visible light reflectance spectra and L＊a＊b＊measurement results. The results show a good correction between lightness and calcium carbonate content in marine-terrigenous deposits;therefore,these deposits can be used as a proxy to study climate changes. Factor-analysis on the first derivative values of the raw visible light reflectance spectra produced three principal factors corresponding to goethite,organic matter,and hematite. Down hole variations in the three fac-tor scores,lightness,calcium carbonate content,and grain size were quite consistent. Moreover,high lightness and low factor scores in goethite,hematite,and organics indicated glacial regression deposits,while low lightness and high factor scores in goethite,hematite,and organics indicated interglacial transgression deposits.