The objective of the present study was to determine the effects of docosahexaenoic acid （DHA）-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 eja...The objective of the present study was to determine the effects of docosahexaenoic acid （DHA）-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk （group Ⅰ）, DHA-enriched hen egg yolk （group Ⅱ）, normal hen egg yolk with 5 mmol L^-1 of cysteine supplementation （group Ⅲ） and DHA-enriched hen egg yolk with 5 mmol L1 of cysteine supplementation （group Ⅳ）. The semen was cryopreserved using controlled rate freezer and was thawed at 50℃ for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone （group Ⅲ） improved progressive motility （P 〈 0.05）, and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk （group Ⅳ） improved both progressive motility （P 〈 0.05） and acrosome integrity （P 〈 0.01）. The use of DHA-enriched hen egg yolk alone （group Ⅱ） did not enhance any of the post-thawed semen qualities （P 〉 0.05）. In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.展开更多
Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. ...Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk （LEY） extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L^-1 （group Ⅰ, control）, 5 mmol L^-1 （group Ⅱ）, 10 mmol L^-1 （group Ⅲ） and 15 mmol L^-1 （group Ⅳ）. Semen suspensions were loaded in straws （0.5 mL） and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher （P 〈 0.01） percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups （group Ⅱ and group Ⅲ） compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group Ⅲ. In conclusion, 5 or 10 mmol L^-1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.展开更多
The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,...The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L-1,while it had negative effects when its concentration was higher than 500 mg·L-1.The hydrogen production was the highest 1.29 mol·mol-1(H2/glucose) when 300 mg·L-1L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP) ,which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range-300 mV to-150 mV when the L-cysteine concentration was higher than 500 mg·L-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.展开更多
The film contained L-cysteine and gold nanoparticles were provided by self-assembled monolayers (SAMs) and potentiostatic electrodeposition technology on the gold electrode. Two methods were used to study the film: In...The film contained L-cysteine and gold nanoparticles were provided by self-assembled monolayers (SAMs) and potentiostatic electrodeposition technology on the gold electrode. Two methods were used to study the film: In the first, cyclic voltammetry (CV) was used to inspect the functional groups of the film and the same time hydroquinone was chosen to be a probe molecule in the based solution;secondly, based on analytical technology of scanning electrochemical microscopy (SECM), the heterogeneous rate constant (keff) between solid phase (the modified electrode) and liquid phase (K3Fe(CN)6) was obtained. As a result, the better binary catalysis of hydroquinone was demonstrated and the heterogeneous rate constant (keff) is the greater at 8 h for L-cysteine self-assembled monolayers (SAMs).展开更多
The effect of L-cysteine in different concentrations on the bioleaching of Ni-Cu sulfide was studied with an extremely thermophilic archaea,Acidianus manzaensis. It is found that adding certain amounts of L-cysteine t...The effect of L-cysteine in different concentrations on the bioleaching of Ni-Cu sulfide was studied with an extremely thermophilic archaea,Acidianus manzaensis. It is found that adding certain amounts of L-cysteine to the bioleaching system of Ni-Cu sulfide largely enhances the leaching rate. X-ray diffraction (XRD) patterns show the change of bioleached solid residues and the effect of L-cysteine on the surface charges of minerals. Zeta potential and IR spectra of mineral surface show that the interaction between L-cysteine and mineral leads to the formation of metal complex,which is propitious to the bioleaching of Ni-Cu sulfide by Acidianus manzaensis.展开更多
Platinum(Pt)/nanofibrous polyaniline(PANI) electrode was prepared by pulse galvanostatic method and characterized by scanning electron microscopy.The electrochemical behavior of L-cysteine at the Pt/nanofibrous PANI e...Platinum(Pt)/nanofibrous polyaniline(PANI) electrode was prepared by pulse galvanostatic method and characterized by scanning electron microscopy.The electrochemical behavior of L-cysteine at the Pt/nanofibrous PANI electrode was investigated by cyclic voltammetry.The results indicate that the pH value of the solution and the Pt loading of the electrode have great effect on the electrocatalytic property of the Pt /nanofibrous PANI electrode;the suitable Pt loading of the electrode is 600 μg/cm2 and the suitable pH value of the solution is 4.5 for investigating L-cysteine oxidation.The L-cysteine sensor based on the Pt/nanofibrous PANI electrode has a good selectivity,reproducibility and stability.The Pt/nanofibrous PANI electrode is highly sensitive to L-cysteine,and the linear calibration curve for the oxidation of L-cysteine can be observed in the range of 0.2-5.0 mmol/L.展开更多
An effective L-cysteine-assisted synthetic route has been successfully developed to prepare copper gallium sulfide（CuGaS2） microspheres under solvothermal conditions with CuCI2.2HzO, GaC13 and L-cysteine as source m...An effective L-cysteine-assisted synthetic route has been successfully developed to prepare copper gallium sulfide（CuGaS2） microspheres under solvothermal conditions with CuCI2.2HzO, GaC13 and L-cysteine as source materials, in which L-cysteine was used as the sulfide source and complexing molecule. The experiments revealed that the synthesized sample was of a typical CuGaS2 tetragonal structure. Moreover, the prepared CuGaS2 crystals consisting of microspheres made up of nanoflakes, and the diameter of the nanoflakes was about 20 nm. Raman spectrum of the obtained CuGaS2 exhibits a high-intensity peak of the A1 mode at 306 cm^-1. Meanwhile, a possible growth mechanism was proposed based on the investigations.展开更多
The effect of L-cysteine of argentum nitrate gel on the wound process of experimental animals is investigated. The results show that the nanogel, being bacteriostatic, accelerates the course of inflammation phase resu...The effect of L-cysteine of argentum nitrate gel on the wound process of experimental animals is investigated. The results show that the nanogel, being bacteriostatic, accelerates the course of inflammation phase resulting in fast cleansing of the wound surface and stimulation of the granulation tissue formation. The main cell elements of this tissue—fibroplasts are characterized by the state of functional excitation. The time of wound healing with the use of L-cysteine of argentum nitrate gel was reduced by three days, and the index of wound repair acceleration was about 20%.展开更多
目的探讨胱抑素C(Cys-C)、餐后2小时C肽(2 h CP)、尿微量白蛋白/尿肌酐(UACR)、病程与2型糖尿病周围神经病变(DPN)的相关性。方法根据双下肢肌电图及足部定量感觉检查结果,选取庆阳市人民医院2021年1月至2022年1月的DPN患者100例作为观...目的探讨胱抑素C(Cys-C)、餐后2小时C肽(2 h CP)、尿微量白蛋白/尿肌酐(UACR)、病程与2型糖尿病周围神经病变(DPN)的相关性。方法根据双下肢肌电图及足部定量感觉检查结果,选取庆阳市人民医院2021年1月至2022年1月的DPN患者100例作为观察组、2型糖尿病非周围神经病变(NDPN)患者73例作为对照组,收集患者的实验室数据及临床指标,分析Cys-C、2 h CP、UACR、病程等指标与DPN之间的相关性。结果DPN组空腹胰岛素(FINS)、尿免疫球蛋白G、尿肌酐、病程、Cys-C、UACR均显著高于NDPN组,差异有统计学意义(t=-2.728,-3.826,-2114,-2.632,-2.850,-2.717,P<0.05);2 h CP水平显著低于NDPN组,差异有统计学意义(t=2.696,P<0.05);Logisitc回归显示,糖尿病病程、2 h CP、Cys-C与DPN具有相关性,差异有统计学意义(OR=1.863,0.729,3.799,P<0.05);ROC曲线显示,病程、Cys-C、2 h CP、糖化血红蛋白(HbA1c)四项联合的诊断性能较好,其次是Cys-C。结论Cys-C、2 h CP、UACR、病程与DPN相关,可能是疾病发作的危险性因素,临床需对此引起高度重视。展开更多
文摘The objective of the present study was to determine the effects of docosahexaenoic acid （DHA）-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk （group Ⅰ）, DHA-enriched hen egg yolk （group Ⅱ）, normal hen egg yolk with 5 mmol L^-1 of cysteine supplementation （group Ⅲ） and DHA-enriched hen egg yolk with 5 mmol L1 of cysteine supplementation （group Ⅳ）. The semen was cryopreserved using controlled rate freezer and was thawed at 50℃ for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone （group Ⅲ） improved progressive motility （P 〈 0.05）, and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk （group Ⅳ） improved both progressive motility （P 〈 0.05） and acrosome integrity （P 〈 0.01）. The use of DHA-enriched hen egg yolk alone （group Ⅱ） did not enhance any of the post-thawed semen qualities （P 〉 0.05）. In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.
文摘Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk （LEY） extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L^-1 （group Ⅰ, control）, 5 mmol L^-1 （group Ⅱ）, 10 mmol L^-1 （group Ⅲ） and 15 mmol L^-1 （group Ⅳ）. Semen suspensions were loaded in straws （0.5 mL） and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher （P 〈 0.01） percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups （group Ⅱ and group Ⅲ） compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group Ⅲ. In conclusion, 5 or 10 mmol L^-1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.
基金Supported by the National High Technology Research and Development Program of China（2006AA02Z246 2007AA03Z456） the National Natural Science Foundation of China（20776119）+2 种基金 the Specialized Research Fund for the Doctoral Program of Higher Education of China（20096101120023） Shaanxi Provincial Natural Science Foundation（SJ08B03） Shaanxi Key Subject Program China
文摘The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L-1,while it had negative effects when its concentration was higher than 500 mg·L-1.The hydrogen production was the highest 1.29 mol·mol-1(H2/glucose) when 300 mg·L-1L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP) ,which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range-300 mV to-150 mV when the L-cysteine concentration was higher than 500 mg·L-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.
文摘The film contained L-cysteine and gold nanoparticles were provided by self-assembled monolayers (SAMs) and potentiostatic electrodeposition technology on the gold electrode. Two methods were used to study the film: In the first, cyclic voltammetry (CV) was used to inspect the functional groups of the film and the same time hydroquinone was chosen to be a probe molecule in the based solution;secondly, based on analytical technology of scanning electrochemical microscopy (SECM), the heterogeneous rate constant (keff) between solid phase (the modified electrode) and liquid phase (K3Fe(CN)6) was obtained. As a result, the better binary catalysis of hydroquinone was demonstrated and the heterogeneous rate constant (keff) is the greater at 8 h for L-cysteine self-assembled monolayers (SAMs).
基金Projects(50621063, 30400010) supported by the National Natural Science Foundation of ChinaProject(2010CB630903) supported by the National Basic Research Program of China
文摘The effect of L-cysteine in different concentrations on the bioleaching of Ni-Cu sulfide was studied with an extremely thermophilic archaea,Acidianus manzaensis. It is found that adding certain amounts of L-cysteine to the bioleaching system of Ni-Cu sulfide largely enhances the leaching rate. X-ray diffraction (XRD) patterns show the change of bioleached solid residues and the effect of L-cysteine on the surface charges of minerals. Zeta potential and IR spectra of mineral surface show that the interaction between L-cysteine and mineral leads to the formation of metal complex,which is propitious to the bioleaching of Ni-Cu sulfide by Acidianus manzaensis.
基金Project(20050532008) supported by the PhD. Program Foundation of Ministry of Education of ChinaProject(06JJ4005) supported by the Natural Science Foundation of Hunan Province+1 种基金 Project(20060400874)supported by the Postdoctoral Foundation of China Project supported by the Postdoctoral Foundation of Hunan University
文摘Platinum(Pt)/nanofibrous polyaniline(PANI) electrode was prepared by pulse galvanostatic method and characterized by scanning electron microscopy.The electrochemical behavior of L-cysteine at the Pt/nanofibrous PANI electrode was investigated by cyclic voltammetry.The results indicate that the pH value of the solution and the Pt loading of the electrode have great effect on the electrocatalytic property of the Pt /nanofibrous PANI electrode;the suitable Pt loading of the electrode is 600 μg/cm2 and the suitable pH value of the solution is 4.5 for investigating L-cysteine oxidation.The L-cysteine sensor based on the Pt/nanofibrous PANI electrode has a good selectivity,reproducibility and stability.The Pt/nanofibrous PANI electrode is highly sensitive to L-cysteine,and the linear calibration curve for the oxidation of L-cysteine can be observed in the range of 0.2-5.0 mmol/L.
基金Supported by the National Natural Science Foundation of China(Nos.507872075 and 50972107)the Project of Key Scientific and Technological Innovations Team of Zhejiang Province,China(No.2009R50010)the Key Foundation of Natural Science Foundation of Zhejiang Province,China(No.Z4110347)
文摘An effective L-cysteine-assisted synthetic route has been successfully developed to prepare copper gallium sulfide（CuGaS2） microspheres under solvothermal conditions with CuCI2.2HzO, GaC13 and L-cysteine as source materials, in which L-cysteine was used as the sulfide source and complexing molecule. The experiments revealed that the synthesized sample was of a typical CuGaS2 tetragonal structure. Moreover, the prepared CuGaS2 crystals consisting of microspheres made up of nanoflakes, and the diameter of the nanoflakes was about 20 nm. Raman spectrum of the obtained CuGaS2 exhibits a high-intensity peak of the A1 mode at 306 cm^-1. Meanwhile, a possible growth mechanism was proposed based on the investigations.
文摘The effect of L-cysteine of argentum nitrate gel on the wound process of experimental animals is investigated. The results show that the nanogel, being bacteriostatic, accelerates the course of inflammation phase resulting in fast cleansing of the wound surface and stimulation of the granulation tissue formation. The main cell elements of this tissue—fibroplasts are characterized by the state of functional excitation. The time of wound healing with the use of L-cysteine of argentum nitrate gel was reduced by three days, and the index of wound repair acceleration was about 20%.
文摘目的探讨胱抑素C(Cys-C)、餐后2小时C肽(2 h CP)、尿微量白蛋白/尿肌酐(UACR)、病程与2型糖尿病周围神经病变(DPN)的相关性。方法根据双下肢肌电图及足部定量感觉检查结果,选取庆阳市人民医院2021年1月至2022年1月的DPN患者100例作为观察组、2型糖尿病非周围神经病变(NDPN)患者73例作为对照组,收集患者的实验室数据及临床指标,分析Cys-C、2 h CP、UACR、病程等指标与DPN之间的相关性。结果DPN组空腹胰岛素(FINS)、尿免疫球蛋白G、尿肌酐、病程、Cys-C、UACR均显著高于NDPN组,差异有统计学意义(t=-2.728,-3.826,-2114,-2.632,-2.850,-2.717,P<0.05);2 h CP水平显著低于NDPN组,差异有统计学意义(t=2.696,P<0.05);Logisitc回归显示,糖尿病病程、2 h CP、Cys-C与DPN具有相关性,差异有统计学意义(OR=1.863,0.729,3.799,P<0.05);ROC曲线显示,病程、Cys-C、2 h CP、糖化血红蛋白(HbA1c)四项联合的诊断性能较好,其次是Cys-C。结论Cys-C、2 h CP、UACR、病程与DPN相关,可能是疾病发作的危险性因素,临床需对此引起高度重视。
