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Identification of novel mammalian viruses in tree shrews(Tupaia belangeri chinensis) 被引量:1
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作者 Hong Zhou Ren-Rong Tian +9 位作者 Xiu-Rong Wang Jin-Xuan Yang Yun-Xiao Wang Ming-Liang Zhao Xu-Dong Zhang Yu-Hua Ma Long-Bao Lv Edward CHolmes Yong-Tang Zheng Wei-Feng Shi 《Zoological Research》 SCIE CSCD 2024年第2期429-438,共10页
The Chinese tree shrew(Tupaia belangeri chinensis),a member of the mammalian order Scandentia,exhibits considerable similarities with primates,including humans,in aspects of its nervous,immune,and metabolic systems.Th... The Chinese tree shrew(Tupaia belangeri chinensis),a member of the mammalian order Scandentia,exhibits considerable similarities with primates,including humans,in aspects of its nervous,immune,and metabolic systems.These similarities have established the tree shrew as a promising experimental model for biomedical research on cancer,infectious diseases,metabolic disorders,and mental health conditions.Herein,we used metatranscriptomic sequencing to analyze plasma,as well as oral and anal swab samples,from 105 healthy asymptomatic tree shrews to identify the presence of potential zoonotic viruses.In total,eight mammalian viruses with complete genomes were identified,belonging to six viral families,including Flaviviridae,Hepeviridae,Parvovirinae,Picornaviridae,Sedoreoviridae,and Spinareoviridae.Notably,the presence of rotavirus was recorded in tree shrews for the first time.Three viruses-hepacivirus 1,parvovirus,and picornavirus-exhibited low genetic similarity(<70%)with previously reported viruses at the whole-genome scale,indicating novelty.Conversely,three other viruses-hepacivirus 2,hepatovirus A and hepevirus-exhibited high similarity(>94%)to known viral strains.Phylogenetic analyses also revealed that the rotavirus and mammalian orthoreovirus identified in this study may be novel reassortants.These findings provide insights into the diverse viral spectrum present in captive Chinese tree shrews,highlighting the necessity for further research into their potential for crossspecies transmission. 展开更多
关键词 Tree shrew(Tupaia belangeri chinensis) Meta-transcriptomic sequencing Mammalian viruses Genomic analysis
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Spatiotemporal dynamics of the social structure of Indo-Pacific humpback dolphins(Sousa chinensis)in Xiamen waters from 2007 to 2019
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作者 Yi Lu Xin-Rong Xu +3 位作者 Bing-Yao Chen Thomas A Jefferson Holly Fearnbach Guang Yang 《Zoological Research》 SCIE CSCD 2024年第2期439-450,共12页
As highly social animals,Indo-Pacific humpback dolphins(Sousa chinensis)exhibit community differentiation.Nevertheless,our understanding of the external and internal factors influencing these dynamics,as well as their... As highly social animals,Indo-Pacific humpback dolphins(Sousa chinensis)exhibit community differentiation.Nevertheless,our understanding of the external and internal factors influencing these dynamics,as well as their spatiotemporal variations,is still limited.In the present study,variations in the social structure of an endangered Indo-Pacific humpback dolphin population in Xiamen Bay,China,were monitored over two distinct periods(2007–2010 and 2017–2019)to analyze the effects of habitat utilization and the composition of individuals within the population.In both periods,the population demonstrated a strikingly similar pattern of social differentiation,characterized by the division of individuals into two main clusters and one small cluster.Spatially,the two primary clusters occupied the eastern and western waters,respectively,although the core distribution area of the eastern cluster shifted further eastward between the two periods.Despite this distribution shift,the temporal stability of the social structure and inter-associations within the eastern cluster remained unaffected.A subset of 16individuals observed in both periods,comprising 51.6%and 43.2%of the population in each respective period,emerged as a foundational element of the social structure and may be responsible for sustaining social structure stability,especially during the 2007–2010 period.These observations suggest that the composition of dominant individuals,an internal factor,had a more substantial influence on the formation of the social network than changes in habitat use,an external factor.Consequently,the study proposes distinct conservation measures tailored to each of the two main clusters. 展开更多
关键词 Social differentiation Social structure Sousa chinensis DYNAMICS CONSERVATION
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Lignans are the main active components of Schisandrae Chinensis Fructus for liver disease treatment:a review
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作者 Ke Fu Shu Dai +6 位作者 Cheng Ma Yafang Zhang Shenglin Zhang Cheng Wang Lihong Gong Honglin Zhou Yunxia Li 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期2425-2444,共20页
As a traditional Chinese herbal medicine,Schisandrae Chinensis Fructus(SC)has been used in medicine and food industry due to its health care and therapeutic effects.Over the past 20 years,the use of SC and its active ... As a traditional Chinese herbal medicine,Schisandrae Chinensis Fructus(SC)has been used in medicine and food industry due to its health care and therapeutic effects.Over the past 20 years,the use of SC and its active ingredient lignans in the prevention and treatment of liver diseases has been increasing,and their hepatoprotective effects has increased the interest of the public and academia.Therefore,in the present work,we first determined the effectiveness of SC in the treatment of liver diseases such as metabolic associated fatty liver disease,alcoholic liver disease,cholestatic liver disease and acute liver injury.Subsequently,the pharmacological effects and molecular mechanisms of lignans,the active components of SC,for liver disease treatment were comprehensively summarized for the first time.The results showed that the lignans in SC could achieve hepatoprotective effects by regulating lipid metabolism,anti-fibrosis,anti-inflammation,anti-oxidation,anti-tumor and regulating bile acid metabolism.The mechanism mainly involved adenosine 5’-monophosphate-activated protein kinase,endoplasmic reticulum stress,sterol regulatory element binding protein 1c,autophagy,transforming growth factor-β,mitogen-activated protein kinase,microRNA,nuclear factor kappa-B,nuclear factor erythroid-2-related factor 2,heat shock proteins and pregnane X receptor signaling pathways.These results can lay a scientific foundation for the development of hepatoprotective drugs or functional foods from SC/lignans. 展开更多
关键词 Schisandrae chinensis Fructus lignans HEPATOPROTECTION Pharmacological effects Molecular mechanism
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Allotetraploidization event of Coptis chinensis shared by all Ranunculales
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作者 Yan Zhang Weina Ge +15 位作者 Jia Teng Yanmei Yang Jianyu Wang Zijian Yu Jiaqi Wang Qimeng Xiao Junxin Zhao Shaoqi Shen Yishan Feng Shoutong Bao Yu Li Yuxian Li Tianyu Lei Yuxin Pan Lan Zhang Jinpeng Wang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期288-303,共16页
Coptis chinensis Franch.,also named Chinese goldthread is a member of Ranunculaceae in the order Ranunculales and represents an important lineage of early eudicots with traditional medicinal value.In our study,by usin... Coptis chinensis Franch.,also named Chinese goldthread is a member of Ranunculaceae in the order Ranunculales and represents an important lineage of early eudicots with traditional medicinal value.In our study,by using syntenic analysis combined with phylogenomic analysis of C.chinensis and four other representative genomes from basal and core eudicots,we confirmed that the WGD event in C.chinensis was shared by Aquilegia coerulea and Papaver somniferum L.and quickly occurred after Ranunculales diverged from other eudicots,likely a Ranunculales common tetraploidization(RCT).The synonymous nucleotide substitutions at synonymous sites distribution of syntenic blocks across these genomes showed that the evolutionary rate of the P.somniferum genome is faster than that of the C.chinensis genome by approximately 13.7%,possibly due to Papaveraceaes having an additional special tetraploidization event(PST).After Ks correction,the RCT dated to 115—130 million years ago(MYA),which was close to the divergence of Ranunculaceaes and Papaveraceaes approximately115.45—130.51 MYA.Moreover,we identified homologous genes related to polyploidization and speciation and constructed multiple sequence alignments with different reference genomes.Notably,the event-related subgenomes in the basal genomes all showed genomic fractionation bias,suggesting a likely allopolyploid nature of the RCT,PST and T-Alpha and T-Beta events in Tetracentron sinense.In addition,we detected that the sixteen P450 subfamilies were markedly expanded in the genomes of Ranunculales,and most of them were related to the RCT and PST events.We constructed a new platform for Early Eudicot Comparative Genomic Research(http://www.cgrpoee.top/index.html)to store more information.In summary,our findings support the WGD of C.chinensis shared by Ranunculales,which is likely an allotetraploidization event.This present effort offered new insights into the evolution of key polyploidization events and the genes related to secondary metabolites during the diversification of early eudicots. 展开更多
关键词 Coptis chinensis RANUNCUlAlES POlYPlOIDIZATION Genomic fractionation P450 genes
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Parent-offspring relationship recognition based on SSR and mtDNA confirmed resource supplement effect of Fenneropenaeus chinensis release
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作者 Song Sun Ding Lyu +2 位作者 Xianshi Jin Xiujuan Shan Weiji Wang 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2024年第2期156-160,共5页
The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and diseases.In order to supplement the fishing yield and restore resources of F.chinensis,the releva... The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and diseases.In order to supplement the fishing yield and restore resources of F.chinensis,the relevant authorities have carried out the activities of stock enhancement and releasing.It can increase biomass and recover resources.However,compared with increasing biomass,there were still few reports on its effect on the recovery of resources.Resource recovery is a process related to whether the released individuals can form a reproductive population.Up to now,there has been a lack of evidence whether the released F.chinensis can complete the entire life history,and form reproduction population.In this study,gravid female shrimp after spawning migration were captured from coastal waters of Haiyang,Qingdao,and Yellow Sea.After identifying parentage relationships using simple sequence repeat(SSR)and mtDNA haplotype,it was finally confirmed that there were eight released individuals in the recapture samples.It was confirmed for the first time that at least part of the released F.chinensis can complete overwintering and reproductive migration,and maintain the migration habits as their wild counterparts.Therefore,we infered that the released shrimp can reproduce under natural conditions,these F.chinensis can form reproductive populations theoretically if without human intervention.These results indicated that enhancenment and release activities have a positive effect on resource recovery. 展开更多
关键词 Fenneropenaeus chinensis RElEASE simple sequence repeat(SSR) mtDNA resource supplement
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Antioxidant and lipoxygenase inhibitory properties of a novel flavonoid from Pistacia chinensis Bunge and its molecular docking analysis
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作者 Abdur Rauf Zuneera Akram +6 位作者 Naveed Muhammad Najla AlMasoud Taghrid Saad Alomar Saima Naz Abdul Wadood Chandni Hayat Marcello Iriti 《Traditional Medicine Research》 2025年第2期30-36,共7页
Background:Pistacia chinensis Bunge has been traditionally used to manage various conditions,including asthma,pain,inflammation,hepatoprotection,and diabetes.The study was conducted to investigate the antioxidant and ... Background:Pistacia chinensis Bunge has been traditionally used to manage various conditions,including asthma,pain,inflammation,hepatoprotection,and diabetes.The study was conducted to investigate the antioxidant and anti-lipoxygenase(LOX)properties of the isolated compound 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one from Pistacia chinensis.Methods:LOX assay and antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl(DPPH)assay were performed.Molecular docking studies were conducted using a molecular operating environment.Results:The LOX assay revealed significant inhibitory effects at 0.2µM concentration,with an IC50 value of 37.80µM.The antioxidant effect demonstrated dose-dependency across 5 to 100µg/mL concentrations,reaching 93.09%at 100µg/mL,comparable to ascorbic acid’s 95.43%effect.Molecular docking studies highlighted strong interactions with the lipoxygenase enzyme,presenting an excellent docking score of-10.98 kcal/mol.Conclusion:These findings provide valuable insights into Pistacia chinensis’chemical components and biological effects,reinforcing its traditional medicinal applications. 展开更多
关键词 Pistacia chinensis Bunge ANTIOXIDANT DPPH assay antilipoxygenase docking analysis
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Summer Warming Limited Bud Output Drives a Decline in Daughter Shoot Biomass through Reduced Photosynthetis of Parent Shoots in Leymus chinensis Seedlings
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作者 Song Gao Ruocheng Xu +4 位作者 Lin Li Jiao Wang Nian Liu Johannes M.H.Knops Junfeng Wang 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第7期1667-1675,共9页
Understanding how summer warming influences the parent and daughter shoot production in a perennial clonal grass is vital for comprehending the response of grassland productivity to global warming.Here,we conducted a s... Understanding how summer warming influences the parent and daughter shoot production in a perennial clonal grass is vital for comprehending the response of grassland productivity to global warming.Here,we conducted a simulated experiment using potted Leymus chinensis,to study the relationship between the photosynthetic activ-ity of parent shoots and the production of daughter shoots under a whole(90 days)summer warming scenario(+3°C).The results showed that the biomass of parents and buds decreased by 25.52%and 33.45%,respectively,under warming conditions.The reduction in parent shoot biomass due to warming directly resulted from decreased leaf area(18.03%),chlorophyll a(18.27%),chlorophyll b(29.21%)content,as well as a reduction in net photosynthetic rate(7.32%)and the maximum quantum efficiency of photosystem II(PSII)photochemistry(4.29%).The decline in daughter shoot biomass was linked to a decrease in daughter shoot number(33.33%)by warming.However,the number of belowground buds increased by 46.43%.The results indicated that long-term summer warming reduces biomass accumulation in parent shoot by increasing both limitation of stoma and non-stoma.Consequently,the parent shoot allocates relatively more biomass to the belowground organs to maintain the survival and growth of buds.Overall,buds,as a potential aboveground population,could remedy for the cur-rent loss of parent shoot density by increasing the number of future daughter shoots if summer warming subsides. 展开更多
关键词 Warming parent shoot bud bank photosynthesis chlorophyllfluorescence leymus chinensis
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Network pharmacology combined with molecular docking revealed the potential targets of Coridius chinensis in prostate cancer treatment
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作者 Mei Zhang Jing Ma +1 位作者 Feng-Yin Zeng Xiao-Hui Hou 《World Journal of Clinical Cases》 SCIE 2024年第27期6094-6104,共11页
BACKGROUND Prostate cancer(PCa)has high morbidity and mortality rates in elderly men.With a history of thousands of years,traditional Chinese medicine derived from insects could be an important source for developing c... BACKGROUND Prostate cancer(PCa)has high morbidity and mortality rates in elderly men.With a history of thousands of years,traditional Chinese medicine derived from insects could be an important source for developing cancer-targeted drugs to prevent tumorigenesis,enhance therapeutic effects,and reduce the risk of recurrence and metastasis.Multiple studies have shown that Coridius chinensis(Cc)has anticancer effects.AIM To elucidate the mechanism of action of Cc against PCa via network pharma-cology and molecular docking.METHODS Potential targets for Cc and PCa were predicted using ChemDraw 19.0 software,the PharmMapper database and the GeneCards database.Then,the STRING database was used to construct the protein–protein interaction network.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment and molecular docking analyses were subsequently conducted to identify the key targets,active ingredients and pathways involved.RESULTS GO and KEGG analyses indicated that the PI3K-Akt signalling pathway was the critical pathway(P value<1.0×10-8).Multiple targeting ingredients that can affect multiple pathways in PCa have been identified in Cc.Seven active compounds(asponguanosines A,asponguanine B,asponguanine C,aspong-pyrazine A,N-acetyldopamine,aspongadenine B and aspongpyrazine B)were selected for molecular docking with 9 potential targets,and the results revealed that aspongpyrazine A and asponguanosine A are the main components by which Cc affects PCa(affinity<-5 kcal/mol,hydrogen bonding),but more studies are needed.CONCLUSION We used network pharmacology to predict the bioactive components and important targets of Cc for the treatment of PCa,supporting the development of Cc as a natural anticancer agent. 展开更多
关键词 Coridius chinensis Molecular docking Network pharmacology Prostate cancer Traditional Chinese medicine
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Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation
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作者 Xu Liang Ni-Hui Zhang +4 位作者 Zhi-Lai Zhan Guang-Lu Chang Yan Gao Xia Li Wen-Yuan Gao 《Traditional Medicine Research》 2025年第2期1-15,共15页
Background:Rosa chinensis Jacq.and Rosa rugosa Thunb.are not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional medicines.The two plants h... Background:Rosa chinensis Jacq.and Rosa rugosa Thunb.are not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional medicines.The two plants have some differences in efficacy,but the flower buds are easily confused for similar traits.In addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal roses.Therefore,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography fingerprints.Then,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large differences.Differential compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and Meigui.Results:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the samples.Through principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four groups.Partial least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavonoid glycosides,as well as phenolic acids.Eight differential components were screened out by orthogonal partial least-squares discriminant analysis and S-plot analysis.Quantitative analyses of the eight compounds,including gallic acid,ellagic acid,hyperoside,isoquercitrin,etc.,showed that Yueji was generally richer in phenolic acids and flavonoids than Meigui,and the quality of Yueji from Shandong and Hebei was better.It is worth noting that Xinjiang rose is rich in various components,which is worth focusing on more in-depth research.Conclusion:In this study,the fingerprints of Meigui and Yueji were established.The chemical components information of roses was further improved based on non-targeted metabolomics and mass spectrometry technology.At the same time,eight differential components of Meigui and Yueji were screened out and quantitatively analyzed.The research results provided a scientific basis for the quality control and rational development and utilization of Rosae Chinensis Flos and Rosae Rugosae Flos,and also laid a foundation for the study of their pharmacodynamic material basis. 展开更多
关键词 Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics CHEMOMETRICS multiple component quantification quality evaluation
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The mechanism of Callistephus chinensis flavonoid compounds in the treatment of diabetes using network pharmacology and molecular docking
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作者 Mingyuan Yuan Xiaoli Wang +1 位作者 Ziqi Sun Xiaoshu Zhang 《Journal of Polyphenols》 2024年第1期11-19,共9页
The purpose of this project is used for exploring the mechanism of Callistephus chinensis in the treatment of diabetes by network pharmacology and molecular docking methods.The target of Callistephus chinensis was obt... The purpose of this project is used for exploring the mechanism of Callistephus chinensis in the treatment of diabetes by network pharmacology and molecular docking methods.The target of Callistephus chinensis was obtained from SwissTargetPrediction database,while the target related to diabetes was obtained from GeneCards and OMIM databases.The target was added in String database to build the protein interaction network.GO biological process enrichment analysis and KEGG pathway enrichment analysis were carried out by Metascape software,then the target-pathway network was constructed.Molecular docking was carried out in Discovery Studio 2016 Client software to verify the binding force of Callistephus chinensis flavonoid compounds with key targets.In this study,10 potential active components were selected from the flavonoid monomer compounds of Callistephus chinensis.1847 biological processes(BP),126 cell compositions(CC)and 256 molecular functions(MF)were obtained by GO enrichment analysis;a total of 194 pathways were involved in KEGG enrichment analysis of 192 cross targets.Network analysis showed that quercetin was the main active component of flavonoids in the treatment of diabetes,AKT1,TNF,VEGFA,EGFR,SRC and other related signals were in relation to the treatment of diabetes.This study showed that Callistephus chinensis flavonoid compounds play a role in the treatment of diabetes by regulating multi-target and multi-pathway. 展开更多
关键词 Callistephus chinensis flavonoid compounds DIABETES network pharmacology molecular docking
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油茶象甲Curculio chinensis基因组Survey测序分析
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作者 张丽 吴佳茜 +2 位作者 曾萝琦 刘惠 汤小宇 《中国农学通报》 2024年第17期135-141,共7页
油茶象甲Curculio chinensis Chevrolat是危害中国特有木本油料树种——油茶的专性蛀果害虫,也是中国林业危险性有害生物之一,在中国不同油茶产区均有分布,对油茶的为害呈加重趋势。为了深入研究其遗传基础和寄主适应性,确定适合鞘翅目... 油茶象甲Curculio chinensis Chevrolat是危害中国特有木本油料树种——油茶的专性蛀果害虫,也是中国林业危险性有害生物之一,在中国不同油茶产区均有分布,对油茶的为害呈加重趋势。为了深入研究其遗传基础和寄主适应性,确定适合鞘翅目象甲科基因组测序研究策略,首先采取Survey测序,之后做深度基因组测序和大规模种群测序。研究采用二代高通量测序技术(MGISEQ-2000),测定了油茶象甲基因组大小,并估计该物种基因组的杂合度、重复率和GC含量。结果显示:油茶象甲基因组大小约为1356.82 Mb,测序深度达到50×;K-mer分析油茶象甲基因组虽然具有杂合峰,但杂合率较低,为1.20%;基因组重复序列含量约为77%。该研究结果对于揭示油茶象甲适应性进化具有重要意义,可为后续构建完整基因组和多种群基因组测序提供策略选择依据。 展开更多
关键词 油茶象甲 鞘翅目 Survey测序 杂合率 GC含量
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316L不锈钢掺杂SiC环状同轴送粉TIG熔覆层组织结构与性能 被引量:1
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作者 高辉 周灿丰 +1 位作者 胡晓慧 李文龙 《焊接学报》 EI CAS CSCD 北大核心 2024年第4期49-56,I0006,共9页
TIG熔覆是经济高效的表面修复方法,与传统的预置粉末法相比,同轴送粉法具有优良的适应性,但是试验研究相对较少.自主设计制造了环状同轴送粉TIG熔覆焊枪,与管状同轴送粉TIG熔覆焊枪相比,制造的熔覆层不存在熄弧位置凹坑、焊缝不够平直... TIG熔覆是经济高效的表面修复方法,与传统的预置粉末法相比,同轴送粉法具有优良的适应性,但是试验研究相对较少.自主设计制造了环状同轴送粉TIG熔覆焊枪,与管状同轴送粉TIG熔覆焊枪相比,制造的熔覆层不存在熄弧位置凹坑、焊缝不够平直以及焊缝熔宽不一致等问题,而且具有更高的熔覆效率.结果表明,采用优化的焊接热输入、送粉量和SiC含量参数匹配,在316L不锈钢表面进行环状同轴送粉TIG熔覆,获得了外观优良的单层单道熔覆层、单层多道熔覆层.对熔覆层进行显微硬度测量、微观组织及元素成分分析、宏观电化学腐蚀试验、微区电化学腐蚀试验以及耐磨性能测试,并与母材进行了比对,环状同轴送粉TIG熔覆导入的SiC粉末有效地提升了熔覆层的耐蚀性与耐磨性. 展开更多
关键词 环状同轴送粉TIG熔覆 316l不锈钢 掺杂SiC 耐蚀性 耐磨性
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苦瓜皂苷L调控PI3K/AKT通路促进内皮祖细胞增殖能力和内皮功能的实验研究
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作者 黄建明 刘滴 +1 位作者 金道群 朱定君 《中西医结合心脑血管病杂志》 2024年第10期1768-1772,共5页
目的:探讨苦瓜皂苷L对内皮祖细胞(EPC)增殖能力和内皮功能的作用及机制。方法:体外分离、培养、鉴定外周血EPC。使用不同浓度苦瓜皂苷L处理EPC 24 h后,应用细胞计数试剂盒(CCK-8)法检测各组细胞活力。使用AutoDock Tools 5.6软件,分别... 目的:探讨苦瓜皂苷L对内皮祖细胞(EPC)增殖能力和内皮功能的作用及机制。方法:体外分离、培养、鉴定外周血EPC。使用不同浓度苦瓜皂苷L处理EPC 24 h后,应用细胞计数试剂盒(CCK-8)法检测各组细胞活力。使用AutoDock Tools 5.6软件,分别以磷脂酰肌醇3-激酶(PI3K)和蛋白激酶B(AKT)为受体,以药物分子苦瓜皂苷L为配体采用盲对接法进行分子对接。使用PI3K/AKT抑制剂LY294002和最佳使用浓度的苦瓜皂苷L单独或同时处理EPC 24 h后检测各组细胞活力和磷酸化PI3K(p-PI3K)、磷酸化AKT(p-AKT)及磷酸化内皮型一氧化氮合酶(p-eNOS)表达水平。结果:与对照组比较,苦瓜皂苷L呈剂量依赖性地促进EPC增殖,且40μmol/L的苦瓜皂苷L是最佳药物浓度。与对照组比较,LY294002可下调EPC细胞活力,苦瓜皂苷L可上调细胞活力。与苦瓜皂苷L组比较,共同使用苦瓜皂苷L和LY294002可降低细胞活力。分子对接结果显示,苦瓜皂苷L与PI3K和AKT均存在直接作用,形成稳定的锁钥结构。与对照组比较,LY294002可显著下调p-PI3K、p-AKT和p-eNOS蛋白水平;苦瓜皂苷L可上调p-PI3K、p-AKT和p-eNOS蛋白水平。与苦瓜皂苷L组比较,使用苦瓜皂苷L和LY294002可降低p-PI3K、p-AKT和p-eNOS蛋白水平。结论:苦瓜皂苷L通过PI3K/AKT信号通路靶向调节EPC的增殖能力,进而上调内皮型一氧化氮合酶(eNOS)活化水平以促进EPC内皮功能。 展开更多
关键词 内皮祖细胞 苦瓜皂苷l 细胞增殖 内皮功能 实验研究
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L型隔板弧形双钢板组合墙板抗爆性能试验与数值研究
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作者 赵春风 何凯城 +1 位作者 李晓杰 王静峰 《工程力学》 EI CSCD 北大核心 2024年第9期79-90,共12页
弧形双钢板混凝土组合墙板结构具有和拱结构相同的传力机理,可以充分发挥混凝土优异的抗压性能,极大地提高了结构的承载力。该文开展了L型隔板弧形双钢板混凝土组合墙板(L-shaped-Curved Double Steel Concrete Composite Wallboard,L-C... 弧形双钢板混凝土组合墙板结构具有和拱结构相同的传力机理,可以充分发挥混凝土优异的抗压性能,极大地提高了结构的承载力。该文开展了L型隔板弧形双钢板混凝土组合墙板(L-shaped-Curved Double Steel Concrete Composite Wallboard,L-CDSCW)在近场爆炸作用下的试验研究,通过混凝土的损伤分析和结构变形分析探究其抗爆性能。基于ANSYS/LS-DYNA软件进行了L-CDSCW试件爆炸响应的数值分析,参数化分析了不同关键参数对L-CDSCW试件抗爆性能的影响规律。结果表明:近场爆炸作用下,L-CDSCW试件未出现倒塌或崩塌现象,具有较强的抗爆性能;增加钢板厚度可以减小混凝土跨中凹陷深度和混凝土塑性破坏;增加TNT当量和降低爆距会显著增加底部钢板跨中最大挠度,同时也会增加混凝土跨中凹陷深度和混凝土损伤。研究结果可以为弧形双钢板混凝土组合板的应用提供一定的技术参考。 展开更多
关键词 近场爆炸 l型隔板弧形双钢板混凝土组合墙板 抗爆性能 试验研究 数值分析
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跗骨窦小切口与经外侧“L”形切口术对跟骨骨折患者的疗效
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作者 张磊 王宏 +2 位作者 张小伟 孟位明 赵志江 《河北医药》 CAS 2024年第15期2295-2299,共5页
目的 比较跗骨窦小切口与经外侧“L”形切口术治疗跟骨骨折的疗效及对足功能恢复情况的影响。方法 研究对象选取2020年10月至2022年10月收治的跟骨骨折患者88例,按照手术方法分为对照组43例,研究组45例,对照组采用经外侧“L”形切口术,... 目的 比较跗骨窦小切口与经外侧“L”形切口术治疗跟骨骨折的疗效及对足功能恢复情况的影响。方法 研究对象选取2020年10月至2022年10月收治的跟骨骨折患者88例,按照手术方法分为对照组43例,研究组45例,对照组采用经外侧“L”形切口术,研究组采用跗骨窦小切口手术。检测比较2组手术情况及并发症发生情况、足功能、跟骨解剖结构、骨代谢、炎性因子、应激反应指标水平。结果 与对照组比较,研究组术后引流量、术中出血量较少,手术、住院及骨折愈合时间较短,并发症发生率较低(P<0.05)。与术前比较,术后3个月2组AOFAS评分、Bohler角、Gissane角、跟骨长度、跟骨高度、P1NP、骨特异性碱性磷酸酶(BALP)水平升高,且研究组高于对照组,抗酒石酸酸性磷酸酶(TRAP)、环磷酰胺(CTX)水平降低,且研究组低于对照组(P<0.05)。与术前比较,术后3 d 2组CRP、IL-6、MDA水平升高,但研究组低于对照组,SOD水平降低,但研究组高于对照组(P<0.05)。结论 跗骨窦小切口术治疗跟骨骨折效果理想,可减少手术创伤,减轻炎症及应激反应,且可促进术后足功能、骨代谢恢复,并发症少。 展开更多
关键词 跟骨骨折 足功能 跗骨窦小切口 经外侧“l”形切口术
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形状系数对20 L球内铝粉分散特性的影响研究
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作者 关文玲 金美华 董呈杰 《中国安全科学学报》 CAS CSCD 北大核心 2024年第8期120-127,共8页
为研究不同形状系数(SF)的铝粉在20 L球型容器中的分散特征,通过建立描述气流携带粉尘形成两相分散系的非稳态数值模型,包括气体流动和粉尘颗粒运动轨迹方程,开展非稳态数值模拟,分析粉尘SF分别为0.2、0.4、0.6、0.8和1.0条件下的粉尘... 为研究不同形状系数(SF)的铝粉在20 L球型容器中的分散特征,通过建立描述气流携带粉尘形成两相分散系的非稳态数值模型,包括气体流动和粉尘颗粒运动轨迹方程,开展非稳态数值模拟,分析粉尘SF分别为0.2、0.4、0.6、0.8和1.0条件下的粉尘空间分布以及球室中心的湍流动能、速度随时间变化。结果表明:球室内气流携带粉尘分散可以分为进粉、扩散、稳定和沉降4个阶段,其分散均匀性和最大速度值随SF的增加而增强;SF越大,即颗粒形状越接近球形,铝粉尘的分散性越好;SF越小,粉尘容易堆积在壁面附近;标称浓度一定时,SF≤0.4,浓度峰值随SF的增加而增加;SF>0.4,浓度峰值随SF的增加而减小;铝粉-空气混合物的湍流动能最大值随SF值的增加而降低;铝粉颗粒的速度峰值随SF值的增加而升高。 展开更多
关键词 形状系数(SF) 20 l 铝粉尘 粉尘质量浓度 分散特性 数值模拟
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20钢/316L复合管固液复合制坯工艺研究 被引量:1
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作者 桂海莲 胡晓彤 +7 位作者 刘浩 张琛 李强 胡建华 陈建勋 苟毓俊 双远华 张鹏岳 《材料研究与应用》 CAS 2024年第1期172-178,共7页
双金属复合管在石油运输行业中应用前景广阔,结合性能是考察复合管是否合格的重要指标之一。为了能在轧制成形过程中得到高品质的复合管材,在复合管制坯过程中要求实现界面处的冶金复合。以20钢和316L为原材料,基于结合温度及元素扩散情... 双金属复合管在石油运输行业中应用前景广阔,结合性能是考察复合管是否合格的重要指标之一。为了能在轧制成形过程中得到高品质的复合管材,在复合管制坯过程中要求实现界面处的冶金复合。以20钢和316L为原材料,基于结合温度及元素扩散情况,采用固液复合方式,并且结合界面的温度模型及通过Pro CAST软件模拟,获得了外模最佳预热温度为1050—1100℃。通过现场试制界面处实现了冶金复合,在最佳温度范围既保证了固态20钢和液态316L之间能达到冶金复合,又能使元素有充分的扩散时间而增强了界面结合强度,同时又可以防止因元素过渡扩散而导致316L腐蚀性能下降。该方法为后续复合管连轧提供了优良的管坯,可实现高品质复合管的现场生产。 展开更多
关键词 固液复合 复合管坯 热传导 界面 结合强度 扩散 20钢/316l 模型
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一种L波段300W GaN脉冲功率模块
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作者 董四华 刘英坤 +1 位作者 高永辉 秦龙 《半导体技术》 CAS 北大核心 2024年第6期555-560,共6页
随着第三代半导体GaN器件技术的不断发展,GaN高电子迁移率晶体管(HEMT)在电子系统中逐步得到了广泛应用。研制了一款小型化L波段300 W GaN脉冲功率模块。研发了满足高压脉冲工作条件的GaN HEMT芯片,采用负载牵引技术进行了器件大信号阻... 随着第三代半导体GaN器件技术的不断发展,GaN高电子迁移率晶体管(HEMT)在电子系统中逐步得到了广泛应用。研制了一款小型化L波段300 W GaN脉冲功率模块。研发了满足高压脉冲工作条件的GaN HEMT芯片,采用负载牵引技术进行了器件大信号阻抗参数提取,并以此为基础设计了小型化匹配网络进行阻抗变换。基于高压驱动芯片和开关器件芯片设计了小型化高压脉冲调制电路。测试结果表明,在工作频率990~1130 MHz、工作电压50 V、脉冲宽度100μs、占空比10%下,功率模块脉冲输出功率大于300 W,功率附加效率大于53%,功率增益大于38 dB。功率模块尺寸为30 mm×30 mm×8 mm。 展开更多
关键词 GaN高电子迁移率晶体管(HEMT) 负载牵引技术 高压脉冲调制 l波段 功率模块
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可用于WLAN的双频MIMO天线设计
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作者 李国金 朱佳辉 《电子元件与材料》 CAS 北大核心 2024年第1期115-120,共6页
设计了一款可用于WLAN的双频MIMO天线。天线的正面为L型单极子,背面为金属接地板。通过在接地板上加载耦合枝节使天线具有双频特性。同时,为了天线的小型化,对耦合枝节进行了弯折处理,减小天线尺寸。在两个天线单元之间加载T型寄生枝节... 设计了一款可用于WLAN的双频MIMO天线。天线的正面为L型单极子,背面为金属接地板。通过在接地板上加载耦合枝节使天线具有双频特性。同时,为了天线的小型化,对耦合枝节进行了弯折处理,减小天线尺寸。在两个天线单元之间加载T型寄生枝节和刻蚀矩形缝隙来提高天线的隔离度。使用电磁仿真软件和矢量网络分析仪对天线进行仿真和实测,结果表明:天线的工作频段为2.4~2.55 GHz和5.1~6.3 GHz,覆盖了WLAN的频段,同时在低频段内隔离度高于19 dB,高频段内隔离度高于25 dB。此外,天线的包络相关系数小于0.01且具有全向辐射特性。天线整体性能较好,可以满足WLAN的需求。 展开更多
关键词 MIMO天线 双频段 无线局域网 l型单极子
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基于高阶幂的单快拍LDACS系统波达方向估计
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作者 王磊 高翔 +1 位作者 胡潇潇 刘海涛 《西北工业大学学报》 EI CAS CSCD 北大核心 2024年第2期362-367,共6页
L波段数字航空通信系统(L band digital aeronautical communication system,LDACS)是未来航空宽带通信重要的基础设施之一,针对LDACS信号容易受到相邻波道大功率测距仪(distance measuring equipment,DME)信号干扰的问题,提出了联合正... L波段数字航空通信系统(L band digital aeronautical communication system,LDACS)是未来航空宽带通信重要的基础设施之一,针对LDACS信号容易受到相邻波道大功率测距仪(distance measuring equipment,DME)信号干扰的问题,提出了联合正交投影干扰抑制与单快拍稀疏分解的波达方向(direction of arrival,DOA)估计方法。通过子空间投影抑制DME干扰,然后使用单快拍数据构建伪协方差矩阵,对伪协方差矩阵求高阶幂,之后进行奇异值分解,并利用约束条件求解稀疏解得到期望信号来向的估计值。所提方法使用高阶伪协方差矩阵降低了噪声影响,仅用单快拍就可以准确估计LDACS信号的入射方向。仿真结果表明,改进单快拍高级幂(improved single snapshot high order power,ISS-HOP)L1-SVD算法的估计精度优于ISS-HOP-MUSIC算法。该方法可以有效抑制DME干扰,提高OFDM接收机性能。 展开更多
关键词 l波段数字航空通信系统 测距仪 波达方向估计 改进单快拍高阶幂算法
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