AIM:To explore the effect of miR-184 and miR-205 on the proliferation and metastasis of conjunctival mucosa associated lymphoid tissue(MALT)lymphoma.METHODS:Tissue of tumor and adjacent normal control from 5 patients ...AIM:To explore the effect of miR-184 and miR-205 on the proliferation and metastasis of conjunctival mucosa associated lymphoid tissue(MALT)lymphoma.METHODS:Tissue of tumor and adjacent normal control from 5 patients with conjunctival MALT was included.RPMI8226 cell line was selected to verify the effect of mi RNAs in B cells.The function of micro RNA on the RPMI8226 cell apoptosis,migration and invasion was evaluated by apoptosis assay and Transwell assay.The m RNA and protein expression were examined by quantitative RT-PCR and Western blotting.The effect of micro RNA on regulation of downstream gene expression was evaluated by luciferase report assay.RESULTS:A decreased level of miR-184 and miR-205 was observed in MALT lymphoma tissue.Exogenous miR-184 and miR-205 analogues promoted apoptosis,and inhibited the survival,migration,and invasion of RPMI8226 cells.miR-184 and miR-205 inhibitor reversed the process.The RNA and protein level of Ras L10 B and TNFAIP8 were downregulated in MALT lymphoma tissue.The exogenous of miR-184 and miR-205 promoted the expression of Ras L10 B and TNFAIP8.Meanwhile,inhibition of miR-184 and miR-205 repressed the expression of target gene,Ras L10 B and TNFAIP8.CONCLUSION:miR-184 and miR-205 suppresses the tumorigenesis of conjunctival MALT lymphoma through regulating Ras L10 B and TNFAIP8.展开更多
Objective: To understand the relationship between the expression of ras and p53 and histological types, degree of differentiation, TNM classification, stage, and patients?prognoses of non-small-cell lung cancer, we ex...Objective: To understand the relationship between the expression of ras and p53 and histological types, degree of differentiation, TNM classification, stage, and patients?prognoses of non-small-cell lung cancer, we examined Ha-ras and p53 production in 143 non-small-cell lung carcinomas. Methods: One hundred and forty-three paraffin-embedded surgically resected specimens of primary non-small-cell lung carcinomas (57 squamous cell carcinomas, 63 acinar adenocarcinomas, 15 bronchio-loalveolar carcinomas, and 8 large-cell carcinomas) were stained by streptavidin-peroxidase immunohistochemical method using anti-Ha-ras monoclonal and anti-p53 monoclonal (DO-1) antibodies. Results: Ha-ras was found in 68% (87 of 143) of lung carcinomas. The positive rate of Ha-ras staining in well differentiated carcinoma was 89%, significantly higher than that in moderately differentiated carcinoma (66%, P<0.05) and that in poorly differentiated carcinoma (48%, P<0.01). The 5-year survival rates of patients whose tumors had no (39%, P<0.01) or moderate (33%, P<0.05) Ha-ras production were significantly higher than that of patients whose tumors had strong staining (14%) for Ha-ras. Sixty percent lung carcinomas (86 of 143) had p53 accumulation. Patients whose tumors did not express p53 survived, on average, significantly longer after tumor resection than did patients whose tumors expressed p53. With increasing p53 accumulation, the average length of survival after tumor resection significantly decreased. Conclusion: Ha-ras overproduction and p53 accumulation correlate with unfavorable prognoses of patients with non-small-cell lung carcinomas. Ha-ras production in non-small- cell lung carcinoma was related to the degree of differentiation.展开更多
ST公司的STM32L072xx系列是超低功耗MCU,具有USB2.0连接功能和高性能ARM CortexM0+32位RISC核,工作频率32MHz,存储器保护单元(MPU),高速嵌入存储器(高达192KB闪存可编存储器,6KB数据EEPROM和20KB RAM)以及增强的I/O和外设。工作电压1.8V...ST公司的STM32L072xx系列是超低功耗MCU,具有USB2.0连接功能和高性能ARM CortexM0+32位RISC核,工作频率32MHz,存储器保护单元(MPU),高速嵌入存储器(高达192KB闪存可编存储器,6KB数据EEPROM和20KB RAM)以及增强的I/O和外设。工作电压1.8V^3.6V,工作温度-40℃^+125℃,主要用在气/水表和工业传感器,健康和健身设备,遥控和用户接口,PC外设,游戏和GPS设备以及告警系统,有线和无线传感器与视频连接.本文介绍了STM32L072xx系列主要特性,框图,以及评估板B-L072Z-LRWAN1 Lo Ra Discovery主要特性,框图,电路图,材料清单和PCB设计图.展开更多
文摘AIM:To explore the effect of miR-184 and miR-205 on the proliferation and metastasis of conjunctival mucosa associated lymphoid tissue(MALT)lymphoma.METHODS:Tissue of tumor and adjacent normal control from 5 patients with conjunctival MALT was included.RPMI8226 cell line was selected to verify the effect of mi RNAs in B cells.The function of micro RNA on the RPMI8226 cell apoptosis,migration and invasion was evaluated by apoptosis assay and Transwell assay.The m RNA and protein expression were examined by quantitative RT-PCR and Western blotting.The effect of micro RNA on regulation of downstream gene expression was evaluated by luciferase report assay.RESULTS:A decreased level of miR-184 and miR-205 was observed in MALT lymphoma tissue.Exogenous miR-184 and miR-205 analogues promoted apoptosis,and inhibited the survival,migration,and invasion of RPMI8226 cells.miR-184 and miR-205 inhibitor reversed the process.The RNA and protein level of Ras L10 B and TNFAIP8 were downregulated in MALT lymphoma tissue.The exogenous of miR-184 and miR-205 promoted the expression of Ras L10 B and TNFAIP8.Meanwhile,inhibition of miR-184 and miR-205 repressed the expression of target gene,Ras L10 B and TNFAIP8.CONCLUSION:miR-184 and miR-205 suppresses the tumorigenesis of conjunctival MALT lymphoma through regulating Ras L10 B and TNFAIP8.
文摘Objective: To understand the relationship between the expression of ras and p53 and histological types, degree of differentiation, TNM classification, stage, and patients?prognoses of non-small-cell lung cancer, we examined Ha-ras and p53 production in 143 non-small-cell lung carcinomas. Methods: One hundred and forty-three paraffin-embedded surgically resected specimens of primary non-small-cell lung carcinomas (57 squamous cell carcinomas, 63 acinar adenocarcinomas, 15 bronchio-loalveolar carcinomas, and 8 large-cell carcinomas) were stained by streptavidin-peroxidase immunohistochemical method using anti-Ha-ras monoclonal and anti-p53 monoclonal (DO-1) antibodies. Results: Ha-ras was found in 68% (87 of 143) of lung carcinomas. The positive rate of Ha-ras staining in well differentiated carcinoma was 89%, significantly higher than that in moderately differentiated carcinoma (66%, P<0.05) and that in poorly differentiated carcinoma (48%, P<0.01). The 5-year survival rates of patients whose tumors had no (39%, P<0.01) or moderate (33%, P<0.05) Ha-ras production were significantly higher than that of patients whose tumors had strong staining (14%) for Ha-ras. Sixty percent lung carcinomas (86 of 143) had p53 accumulation. Patients whose tumors did not express p53 survived, on average, significantly longer after tumor resection than did patients whose tumors expressed p53. With increasing p53 accumulation, the average length of survival after tumor resection significantly decreased. Conclusion: Ha-ras overproduction and p53 accumulation correlate with unfavorable prognoses of patients with non-small-cell lung carcinomas. Ha-ras production in non-small- cell lung carcinoma was related to the degree of differentiation.
文摘ST公司的STM32L072xx系列是超低功耗MCU,具有USB2.0连接功能和高性能ARM CortexM0+32位RISC核,工作频率32MHz,存储器保护单元(MPU),高速嵌入存储器(高达192KB闪存可编存储器,6KB数据EEPROM和20KB RAM)以及增强的I/O和外设。工作电压1.8V^3.6V,工作温度-40℃^+125℃,主要用在气/水表和工业传感器,健康和健身设备,遥控和用户接口,PC外设,游戏和GPS设备以及告警系统,有线和无线传感器与视频连接.本文介绍了STM32L072xx系列主要特性,框图,以及评估板B-L072Z-LRWAN1 Lo Ra Discovery主要特性,框图,电路图,材料清单和PCB设计图.
