Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR （1H-1H COSY, HMBC and ...Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR （1H-1H COSY, HMBC and NOESY） as 1β-O-β-D- glucopyranosyl-4α-hydroxyl-5α, 6β, 11βH-eudesma-12, 6α-olide （1） and 1β-hydroxyl-15-O-（p-methoxyphenylacetyl）-5α, 6β, 11 βH-eudesma-3-en- 12, 6a-olide （2）.展开更多
The components of the essential oils obtained from different parts of Daacus carota L.var. saliva Hoffm were analyzed. The percentages of the essential oils extracted are 0. 27% （ mL/100 g material） for the flowers,...The components of the essential oils obtained from different parts of Daacus carota L.var. saliva Hoffm were analyzed. The percentages of the essential oils extracted are 0. 27% （ mL/100 g material） for the flowers, 0. 0/% for the stems and leaves and 0. 01% for the roots. Fifty-four, Sixty-six and Thirty-three compounds were,respectively, separated and identified from the flowers, the stems and leaves and the roots, among which unsaturated alkene compounds are thirty-nine, thirty-nine and twenty-one, respectively, accounting in turn for up to 90. 21%, 90. 49% and 72. 65% of the total essential oils. Because alkene compounds have double bonds that are easily oxidized, it can he inferred that the components of the essential oils in the different parts of Daucus carota L. var. saliva Hoffm should show an activity of the antioformation of free radicals to some extent.展开更多
Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragment...Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.展开更多
Canola and oat forage potential may be affected by climatic conditions when sown early.The objective of this study was to evaluate the forage canola and oat potential in four early sowing dates(September 11 and 25;Oct...Canola and oat forage potential may be affected by climatic conditions when sown early.The objective of this study was to evaluate the forage canola and oat potential in four early sowing dates(September 11 and 25;October 9 and 23)during the 2012-2013 and 2013-2014 cycles in Matamoros,Coahuila,Mexico.Growth cycle duration,chemical composition,dry matter(DM),crude protein(CP),and net energy for lactation(NEL)yields were determined.High temperatures and long photoperiods affected crops seeded on September 11,accelerating growth and reducing canola(26.6%-31.7%)and oat(15.8%)DM yields.As of September 25,canola cv IMC 205 reached DM yields(7746 kg ha^(-1)-9276 kg ha^(-1))similar to those obtained by oat(8115 kg ha^(-1)-9507 kg ha^(-1)),while canola cv Hyola 401 obtained such yields only until October 23.Canola chemical composition was better than that found in oat,with higher CP,but lower acid detergent fiber(ADF)and neutral detergent fiber(NDF)contents.Canola equaled oat CP yields(972 kg ha^(-1)-1215 kg ha^(-1))in the first sowing date,while in the other three other canola sowings reached higher yields(1193 kg ha^(-1)-1889 kg ha^(-1)).As for NEL yields,no difference was observed between both species.The best sowing date for canola is from September 25 on,with CP production advantages over oat.展开更多
以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+...以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)]生菜的株高、地上部鲜重、地上部干重、根系活力、地下部鲜重、地下部干重和产量均显著高于CK,比CK分别提高了18.27%、61.04%、88.20%、101.43%、59.34%、125.00%和64.74%,处理M2Y2生菜的可溶性糖、可溶性蛋白质、维生素C含量也明显高于CK,较CK分别增加了63.59%、42.35%和117.32%,而亚硝酸盐含量则显著低于CK,比CK降低了66.07%。施用效果最好的为处理M2Y2,其次为处理M1Y2,即底施木美土里微生物菌肥600 kg/hm2(M1)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)。展开更多
基金financed by the Science Foundation of Zhejiang Sci-Tech University(No.0613266-Y)the Talents Training Foundation of Key Laboratory of Advanced Textile Materials and Manufacturing Technology (Zhejiang Sci-Tech University),Ministry of Education(No.2006QN04)
文摘Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR （1H-1H COSY, HMBC and NOESY） as 1β-O-β-D- glucopyranosyl-4α-hydroxyl-5α, 6β, 11βH-eudesma-12, 6α-olide （1） and 1β-hydroxyl-15-O-（p-methoxyphenylacetyl）-5α, 6β, 11 βH-eudesma-3-en- 12, 6a-olide （2）.
文摘The components of the essential oils obtained from different parts of Daacus carota L.var. saliva Hoffm were analyzed. The percentages of the essential oils extracted are 0. 27% （ mL/100 g material） for the flowers, 0. 0/% for the stems and leaves and 0. 01% for the roots. Fifty-four, Sixty-six and Thirty-three compounds were,respectively, separated and identified from the flowers, the stems and leaves and the roots, among which unsaturated alkene compounds are thirty-nine, thirty-nine and twenty-one, respectively, accounting in turn for up to 90. 21%, 90. 49% and 72. 65% of the total essential oils. Because alkene compounds have double bonds that are easily oxidized, it can he inferred that the components of the essential oils in the different parts of Daucus carota L. var. saliva Hoffm should show an activity of the antioformation of free radicals to some extent.
基金Supported by Natural Science Foundation of Yunnan Province(2011FB049)National Natural Science Foundation of China(31260481,31460516)+2 种基金Fund of Yunnan Education Department(2013Y251)Fund of the Department of Life Science and Technology,Kunming University(GXKM201505)Talent Fund for PhD(YJL11015)
文摘Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.
基金This research was funded by the Instituto Nacional de Investigaciones Forestales,Agrícolas y Pecuarias(México).
文摘Canola and oat forage potential may be affected by climatic conditions when sown early.The objective of this study was to evaluate the forage canola and oat potential in four early sowing dates(September 11 and 25;October 9 and 23)during the 2012-2013 and 2013-2014 cycles in Matamoros,Coahuila,Mexico.Growth cycle duration,chemical composition,dry matter(DM),crude protein(CP),and net energy for lactation(NEL)yields were determined.High temperatures and long photoperiods affected crops seeded on September 11,accelerating growth and reducing canola(26.6%-31.7%)and oat(15.8%)DM yields.As of September 25,canola cv IMC 205 reached DM yields(7746 kg ha^(-1)-9276 kg ha^(-1))similar to those obtained by oat(8115 kg ha^(-1)-9507 kg ha^(-1)),while canola cv Hyola 401 obtained such yields only until October 23.Canola chemical composition was better than that found in oat,with higher CP,but lower acid detergent fiber(ADF)and neutral detergent fiber(NDF)contents.Canola equaled oat CP yields(972 kg ha^(-1)-1215 kg ha^(-1))in the first sowing date,while in the other three other canola sowings reached higher yields(1193 kg ha^(-1)-1889 kg ha^(-1)).As for NEL yields,no difference was observed between both species.The best sowing date for canola is from September 25 on,with CP production advantages over oat.
文摘以生菜(Lactuca sativa L. var. ramosa Hort.)为试验材料,研究了微生物菌肥对冀西北坝上地区生菜产量和品质的影响。结果表明,施用菌肥能促进生菜生长,提高其产量,改善其品质;其中,处理M2Y2[底施木美土里微生物菌肥1 200 kg/hm2(M2)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)]生菜的株高、地上部鲜重、地上部干重、根系活力、地下部鲜重、地下部干重和产量均显著高于CK,比CK分别提高了18.27%、61.04%、88.20%、101.43%、59.34%、125.00%和64.74%,处理M2Y2生菜的可溶性糖、可溶性蛋白质、维生素C含量也明显高于CK,较CK分别增加了63.59%、42.35%和117.32%,而亚硝酸盐含量则显著低于CK,比CK降低了66.07%。施用效果最好的为处理M2Y2,其次为处理M1Y2,即底施木美土里微生物菌肥600 kg/hm2(M1)+叶面喷施育苗宝贝微生物菌剂1∶200(Y2)。
