目的探讨决定Lactobacillus plantarum Lp6在小鼠小肠派伊尔结(PP)内化的细菌源因素,并分析该菌株的免疫调节作用。方法FITC标记不同处理的细菌,分析在含PPs的回肠结扎段内化的情况。给小鼠灌胃活或灭活细菌,研究其对小鼠脾、PP细胞增...目的探讨决定Lactobacillus plantarum Lp6在小鼠小肠派伊尔结(PP)内化的细菌源因素,并分析该菌株的免疫调节作用。方法FITC标记不同处理的细菌,分析在含PPs的回肠结扎段内化的情况。给小鼠灌胃活或灭活细菌,研究其对小鼠脾、PP细胞增殖和腹腔巨噬细胞吞噬活性的影响。结果甘露糖可以强烈的抑制细菌内化,灭活和四甲基脲处理可以较明显的减低细菌内化。活菌和灭活菌可以特异性的调节腹腔巨噬细胞、PP细胞和脾淋巴细胞的活性。结论细菌表面甘露糖糖凝集素是影响细菌PP内化的最主要因素。细菌活力、表面疏水性也有一定的作用。活菌和灭活可以不同程度的增强小鼠腹腔巨噬细胞的吞噬活性并抑制脾和PP淋巴细胞增殖。展开更多
[ Objective] This study aimed to investigate the regulatory effects of xylooligesaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice. [Method] In the intestinal microbial flora prolife...[ Objective] This study aimed to investigate the regulatory effects of xylooligesaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice. [Method] In the intestinal microbial flora proliferation regulation experiment, microflom quantities of Bifidobacterium, Lactobacillus, Enterobacter and Enterococci in excrements of BALB/c mice were determined after continuous administration of high-dose[ 1.0 g/（ kg ~ bw ） ] and low-dose[ 0.5 g/（ kg ~ bw ） ] xylooligosaccharides for 14 d. In the defecation regulation experiment, constipation model of BALB/c mice was established with compound diphenexylate after con- tinuous administration of high-dose and low-dose xylooligosaccharides for 21 d, to determine the first defecation time of black excrement, weight of black excrements within 6 h and rate of intestinal propulsion of constipation mice. [ Result] Compared with the control group, after continuous administration of xyloolignsaccharides for 14 d, microflora quantifies of Lactobac//lus and B/fidobacter/um in excrements of BALB/c mice in low-dose and high-dose groups both increased significantly （ P 〈 0.05 ）. After continuous administration of xylooligosaccharidos, microflora quantities of Enterococci and Lactobacillus in control group were significantly raised （P 〈 O. 05 and P 〈 0.01, respectively） compared with those before administration; mieroflora quantities of Enterococci in high-dose and low-dose groups were signif- icantly reduced （P 〈0.05）, while microflora quantities of/zwtobac//lus and B/fidobacter/um and the B/E value increased significantly （P 〈0. 05, P 〈0.01 and P 〈 0.01, respectively）. After continuous administration of xylooligosaccharides for 21 d, compared with the model group, the first defecation time of black excre- ment in high-dose and low-dose groups was significantly shortened （ P 〈 O. 05 ）, the total weight of black.excrements within 6 h increased significantly （ P 〈 0.05 ） and the rate of intestinal propulsion was significantly enhanced （ P 〈0.01 ）. [ Conclusion ] Low-dose （0.5 g/Kg. bw） and high-dose （ 1 g/Kg. bw） xylooligosac- charides can improve the proliferation of intestinal microbial flora and prevent mice from constipation.展开更多
文摘目的探讨决定Lactobacillus plantarum Lp6在小鼠小肠派伊尔结(PP)内化的细菌源因素,并分析该菌株的免疫调节作用。方法FITC标记不同处理的细菌,分析在含PPs的回肠结扎段内化的情况。给小鼠灌胃活或灭活细菌,研究其对小鼠脾、PP细胞增殖和腹腔巨噬细胞吞噬活性的影响。结果甘露糖可以强烈的抑制细菌内化,灭活和四甲基脲处理可以较明显的减低细菌内化。活菌和灭活菌可以特异性的调节腹腔巨噬细胞、PP细胞和脾淋巴细胞的活性。结论细菌表面甘露糖糖凝集素是影响细菌PP内化的最主要因素。细菌活力、表面疏水性也有一定的作用。活菌和灭活可以不同程度的增强小鼠腹腔巨噬细胞的吞噬活性并抑制脾和PP淋巴细胞增殖。
基金Supported by Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PHR201008293)
文摘[ Objective] This study aimed to investigate the regulatory effects of xylooligesaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice. [Method] In the intestinal microbial flora proliferation regulation experiment, microflom quantities of Bifidobacterium, Lactobacillus, Enterobacter and Enterococci in excrements of BALB/c mice were determined after continuous administration of high-dose[ 1.0 g/（ kg ~ bw ） ] and low-dose[ 0.5 g/（ kg ~ bw ） ] xylooligosaccharides for 14 d. In the defecation regulation experiment, constipation model of BALB/c mice was established with compound diphenexylate after con- tinuous administration of high-dose and low-dose xylooligosaccharides for 21 d, to determine the first defecation time of black excrement, weight of black excrements within 6 h and rate of intestinal propulsion of constipation mice. [ Result] Compared with the control group, after continuous administration of xyloolignsaccharides for 14 d, microflora quantifies of Lactobac//lus and B/fidobacter/um in excrements of BALB/c mice in low-dose and high-dose groups both increased significantly （ P 〈 0.05 ）. After continuous administration of xylooligosaccharidos, microflora quantities of Enterococci and Lactobacillus in control group were significantly raised （P 〈 O. 05 and P 〈 0.01, respectively） compared with those before administration; mieroflora quantities of Enterococci in high-dose and low-dose groups were signif- icantly reduced （P 〈0.05）, while microflora quantities of/zwtobac//lus and B/fidobacter/um and the B/E value increased significantly （P 〈0. 05, P 〈0.01 and P 〈 0.01, respectively）. After continuous administration of xylooligosaccharides for 21 d, compared with the model group, the first defecation time of black excre- ment in high-dose and low-dose groups was significantly shortened （ P 〈 O. 05 ）, the total weight of black.excrements within 6 h increased significantly （ P 〈 0.05 ） and the rate of intestinal propulsion was significantly enhanced （ P 〈0.01 ）. [ Conclusion ] Low-dose （0.5 g/Kg. bw） and high-dose （ 1 g/Kg. bw） xylooligosac- charides can improve the proliferation of intestinal microbial flora and prevent mice from constipation.
