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乌梅提取液对李斯特菌的抑菌机理 被引量:27
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作者 耿飞 王伟 周涛 《食品科学》 EI CAS CSCD 北大核心 2011年第15期88-93,共6页
运用流式细胞仪研究乌梅提取液对李斯特菌的抑菌机理。首先通过抑菌实验确定乌梅提取液对李斯特菌的最小抑菌浓度(MIC)为0.625mg/mL,然后用1、2、4倍MIC的乌梅提取液对李斯特菌进行处理,通过流式细胞仪对其前向散射光(FSC)变化、DNA含... 运用流式细胞仪研究乌梅提取液对李斯特菌的抑菌机理。首先通过抑菌实验确定乌梅提取液对李斯特菌的最小抑菌浓度(MIC)为0.625mg/mL,然后用1、2、4倍MIC的乌梅提取液对李斯特菌进行处理,通过流式细胞仪对其前向散射光(FSC)变化、DNA含量变化、细胞膜电位变化、细胞周期变化和Annexin V-FITC/PI双标记法、细胞内Ca2+含量变化指标进行检测,探索抑菌机理。结果表明,乌梅提取液通过破坏李斯特菌的细胞膜系统和阻滞DNA的合成抑制其生长繁殖。 展开更多
关键词 流式细胞仪 乌梅 李斯特菌 抑菌机理
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Nisin Use to Control L. innocua in Experimentally Contaminated Gombasek Sausage
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作者 A. Laukova P. Turek J. Nagy 《Journal of Agricultural Science and Technology(A)》 2011年第5期746-750,共5页
Meat is a rich nutrient matrix that provides a suitable environment for proliferation of meat spoilage microbiota and common food-bom pathogens, therefore adequate preservation has to be applied in order to preserve i... Meat is a rich nutrient matrix that provides a suitable environment for proliferation of meat spoilage microbiota and common food-bom pathogens, therefore adequate preservation has to be applied in order to preserve its safety and qualib:. Lantibiotic nisin is not conunonly used in the meat industry, in spite of its beneficial effect in e.g. dairy industry. Theretbre, here, the effect of nisin was searched in traditional fermented sausage Gombasek experimentally contaminated with Listeria innocua Lil strata Ihe antimicrobial effect of nisin was demonstrated by the Lil strain decrease at day 2 (after contamination) with the difference 1.79 logarithmic (log) cycles comparing with the control sausage (CLi). At week l, the difference 4.14 log cycles was noted in the experimental sausages comparing with CLi sausages with prolonging inhibitory effect up to week 2; although the difference was diminished (1.82 log cycles); but higher than at day 2, The pH values as well as lactic acid values were not influenced by the nisin treatment (pH value in the reference sausage was 5.0, in CLi 5.7 and in the experimental sausage E, 5.1; amount of lactic acid in t: was 0.099 mmol L^-1: and in CLi 0.107 mmol L^-1). 展开更多
关键词 NISIN SAUSAGE L. innocua CONTROL
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