[ Objective ] This study aimed to confirm the roles of cell wall degrading enzymes （CWDEs） produced by Botryodiplodia theobromae Pat. in the infec- tion of mango fruits. [ Method] Change of activities of five types ...[ Objective ] This study aimed to confirm the roles of cell wall degrading enzymes （CWDEs） produced by Botryodiplodia theobromae Pat. in the infec- tion of mango fruits. [ Method] Change of activities of five types of CWDEs produced by B. theobromae Pat. were studied under both in vitro culture and inocula- tion conditions, along with the pathogenicity and the ability of producing CWDEs of four post-harvest fangal pathogens（B, theobromae Pat. , Colletotrichum gloeos- porioides Penz. , Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet. et Cif. ） which cause stem-end rot of mangoes. [ Result] B. theobromae Pat. was a- ble to produce polygalacturonase（PG）, pectinmethylgalacturonase（PMG）, polygalacturonic acid trans-eliminase （PGTE）, pectin methyltrans-eliminase （PMTE） and cellulase （ Cx. ） under both in vitro culture and inoculation conditions, of which activities of PG, Cx and PMG were significantly higher in than that in either PGTE or PMTE. Among three primary CWDEs, the peak of activities of PG and Cx appeared earlier and that of PMG occured later. The pathogenicity of B. theo- bromae Pat. was significantly higher than that of any other three pathogens; it is the same with the abilities of producing pectinase. [ Conclusion] This paper pro- vides theoretical bases for further exploring the mechanism of host-pathogen interaction and decreasing the post-harvest loss of mango fruits.展开更多
利用15对SSR引物,分析具有广泛来源的70份可可资源的遗传多样性。结果表明,15对引物共扩增出76个条带,其中50个多态性条带,占总带数的65.8%。70份可可资源间遗传相似系数（SM）在0.341~0.943之间,平均值为0.625,说明可可资源具有丰富的...利用15对SSR引物,分析具有广泛来源的70份可可资源的遗传多样性。结果表明,15对引物共扩增出76个条带,其中50个多态性条带,占总带数的65.8%。70份可可资源间遗传相似系数（SM）在0.341~0.943之间,平均值为0.625,说明可可资源具有丰富的遗传多样性。在相似系数为0.65水平上,可将70份可可资源分成10类。利用Structure 2.3.2软件分析群体结构,结合可可果实相关性状的表型数据,采用Tassel 2.1的一般线性模型（General linear model,GLM）进行关联分析;结果表明18个位点与果重、果壳重、果长、果径围、果壳厚显著相关（p〈0.05）,各位点对表型变异贡献率为5.5%~13.1%。展开更多
本试验采用5 s rDNA间隔序列分析和SRAP标记验证以色列野生二粒小麦与光稃野燕麦远缘杂种的真实性。结果表明,引物对PⅠ/PⅡ在以色列野生二粒小麦与光稃野燕麦杂种中扩增出了父本特异带,父本的遗传物质导入了小麦基因组。经SRAP标记分析...本试验采用5 s rDNA间隔序列分析和SRAP标记验证以色列野生二粒小麦与光稃野燕麦远缘杂种的真实性。结果表明,引物对PⅠ/PⅡ在以色列野生二粒小麦与光稃野燕麦杂种中扩增出了父本特异带,父本的遗传物质导入了小麦基因组。经SRAP标记分析,引物对me 3-em3在该杂种中也扩增出了父本特异带,进一步证明了杂种的真实性。展开更多
根据野外采样和文献查阅,系统整理了可可的地理分布记录,并利用MaxEnt生态位模型和ArcGIS软件对可可的潜在适宜分布范围进行预测。结果表明:北美洲南部、中南美洲北部、非洲西部、亚洲东南部以及太平洋美拉尼西亚群岛地区均是可可的潜...根据野外采样和文献查阅,系统整理了可可的地理分布记录,并利用MaxEnt生态位模型和ArcGIS软件对可可的潜在适宜分布范围进行预测。结果表明:北美洲南部、中南美洲北部、非洲西部、亚洲东南部以及太平洋美拉尼西亚群岛地区均是可可的潜在适宜分布区域。其中,中国海南、台湾南部、云南西双版纳、广东雷州半岛也属于可可的适生范围。经ROC(Receiver operating characteristic)曲线分析法验证,MaxEnt模型的AUC(Area under curve)值为0.977,表明预测结果具有较高的可信度。各环境变量重要性的Jacknife检验表明,极端最低温度、年降雨量、年温度变化范围、最暖季降雨量对可可的潜在分布影响最大。展开更多
基金Supported by the Special Fund for Agro-scientific Research(3-44-2)the Special Fund for Basic Research of Central Universities in the Public Interest,China(N0.2009hzs1J011,NO.2011hzs1J027,NO.2011hzs1J004)
文摘[ Objective ] This study aimed to confirm the roles of cell wall degrading enzymes （CWDEs） produced by Botryodiplodia theobromae Pat. in the infec- tion of mango fruits. [ Method] Change of activities of five types of CWDEs produced by B. theobromae Pat. were studied under both in vitro culture and inocula- tion conditions, along with the pathogenicity and the ability of producing CWDEs of four post-harvest fangal pathogens（B, theobromae Pat. , Colletotrichum gloeos- porioides Penz. , Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet. et Cif. ） which cause stem-end rot of mangoes. [ Result] B. theobromae Pat. was a- ble to produce polygalacturonase（PG）, pectinmethylgalacturonase（PMG）, polygalacturonic acid trans-eliminase （PGTE）, pectin methyltrans-eliminase （PMTE） and cellulase （ Cx. ） under both in vitro culture and inoculation conditions, of which activities of PG, Cx and PMG were significantly higher in than that in either PGTE or PMTE. Among three primary CWDEs, the peak of activities of PG and Cx appeared earlier and that of PMG occured later. The pathogenicity of B. theo- bromae Pat. was significantly higher than that of any other three pathogens; it is the same with the abilities of producing pectinase. [ Conclusion] This paper pro- vides theoretical bases for further exploring the mechanism of host-pathogen interaction and decreasing the post-harvest loss of mango fruits.
文摘利用15对SSR引物,分析具有广泛来源的70份可可资源的遗传多样性。结果表明,15对引物共扩增出76个条带,其中50个多态性条带,占总带数的65.8%。70份可可资源间遗传相似系数（SM）在0.341~0.943之间,平均值为0.625,说明可可资源具有丰富的遗传多样性。在相似系数为0.65水平上,可将70份可可资源分成10类。利用Structure 2.3.2软件分析群体结构,结合可可果实相关性状的表型数据,采用Tassel 2.1的一般线性模型（General linear model,GLM）进行关联分析;结果表明18个位点与果重、果壳重、果长、果径围、果壳厚显著相关（p〈0.05）,各位点对表型变异贡献率为5.5%~13.1%。
文摘本试验采用5 s rDNA间隔序列分析和SRAP标记验证以色列野生二粒小麦与光稃野燕麦远缘杂种的真实性。结果表明,引物对PⅠ/PⅡ在以色列野生二粒小麦与光稃野燕麦杂种中扩增出了父本特异带,父本的遗传物质导入了小麦基因组。经SRAP标记分析,引物对me 3-em3在该杂种中也扩增出了父本特异带,进一步证明了杂种的真实性。
文摘根据野外采样和文献查阅,系统整理了可可的地理分布记录,并利用MaxEnt生态位模型和ArcGIS软件对可可的潜在适宜分布范围进行预测。结果表明:北美洲南部、中南美洲北部、非洲西部、亚洲东南部以及太平洋美拉尼西亚群岛地区均是可可的潜在适宜分布区域。其中,中国海南、台湾南部、云南西双版纳、广东雷州半岛也属于可可的适生范围。经ROC(Receiver operating characteristic)曲线分析法验证,MaxEnt模型的AUC(Area under curve)值为0.977,表明预测结果具有较高的可信度。各环境变量重要性的Jacknife检验表明,极端最低温度、年降雨量、年温度变化范围、最暖季降雨量对可可的潜在分布影响最大。
