房产税对上海房价的调控效应分析——基于LLS模型的实证研究

目前房产税对房价影响的研究仍没有获得一致的结论。文章借鉴行为金融学LLS模型,并对该模型进行优化的基础上,以上海市为研究对象,分别模拟了完全市场调节和房产税政策调控下的房价走势,并从统计学角度对房价走势进行了数据分析和比较。LLS模型分析表明,目前房产税征收税率低,对房价的抑制作用较小,房产税税率的杠杆作用并不显著,但对房价的抑制作用将逐年增强,这为深化我国房产税改革提供了有益的借鉴与决策支持。

老年慢性心力衰竭患者血清LL37和SSC5D水平检测对预后的评估价值

目的探讨血清抗菌肽LL37(LL37)和人可溶性富含半胱氨酸结构域的清道夫受体蛋白(soluble scavengerreceptor cysteine-rich domain-containing protein,SSC5D)在老年慢性心力衰竭(chronic heart failure,CHF)中的表达及对预后的评估价值。方法选取上海交通大学医学院附属苏州九龙医院2017年1月~2022年1月收治的100例CHF患者为CHF组,根据预后情况将患者分为预后良好组(n=60)和预后不良组(n=40)。另选取100例健康体检者为对照组。酶联免疫吸附试验(ELISA)检测血清LL37和SSC5D水平。比较CHF组和对照组血清LL37和SSC5D水平差异;Spearman分析血清LL37和SSC5D相关性;Kaplan-Meier生存曲线分析血清LL37和SSC5D表达与CHF患者预后的关系;COX分析影响CHF患者预后的因素。采用ROC曲线分析血清LL37和SSC5D对老年CHF患者预后不良的预测价值。结果CHF组血清LL37(771.38±158.25 ng/ml)和SSC5D(15789.35±1306.25 pg/ml)水平高于对照组(526.23±115.58ng/ml,8938.72±858.29 pg/ml),差异具有统计学意义(t=12.510,43.830,均P<0.001)。Spearman相关性分析结果显示,CHF患者血清LL37与SSC5D水平呈显著正相关(r=0.629,P<0.001);CHF患者血清LL37,SSC5D水平与NYHA心功能分级呈显著正相关(r=0.776,0.751,均P<0.001)。高水平LL37组生存率显著低于低水平LL37组(38.18%vs 86.67%),差异具有统计学意义(Log rankχ^(2)=24.242,P<0.001);高水平SSC5D组生存率显著低于低水平SSC5D组(37.74%vs 85.10%),差异具有统计学意义(Log rankχ^(2)=23.291,P<0.001)。预后不良组>70岁占比、NYHA心功能分级Ⅲ+Ⅳ级占比、血清LL37和SSC5D水平高于预后良好组,差异具有统计学意义(χ^(2)=10.774,4.118,t=4.723,14.059,均P<0.05)。多因素COX分析结果表明,年龄>70岁(OR=1.515,95%CI:1.224~1.858)、NYHA心功能Ⅲ+Ⅳ分级(OR=1.236,95%CI:1.198~1.963)、高水平LL37(OR=1.705,95%CI:1.163~2.582)和高水平SSC5D(OR=1.591,95%CI:1.052~1.916)是影响CHF患者预后的独立危险因素(均P<0.05)。ROC曲线分析显示,血清LL37和SSC5D联合预测老年CHF预后不良的曲线下面积大于血清LL37和SSC5D单独检测,差异具有统计学意义(Z=2.834,2.168,P=0.005,0.030)。结论CHF患者血清LL37和SSC5D水平升高,两者均是CHF患者预后不良危险因素,可作为临床评估CHF预后指标。

紫草素联合肿瘤细胞裂解物刺激DC疫苗的抗肿瘤效果

目的:探讨紫草素(SK)联合肿瘤细胞裂解物(TCL)刺激的DC疫苗的抗肿瘤效果。方法:体外制备SK和TCL刺激的正常小鼠源的DC疫苗,流式细胞术检测DC表面CD80、CD86的荧光强度。流式细胞术检测与SK+TCL刺激的DC共培养后的正常小鼠脾T细胞中T-bet和RORγt的表达,ELISA检测共培养上清液中IFN-γ、IL-12P70和TNF-α的含量。建立Lewis肺癌3LL细胞荷瘤小鼠模型,分为PBS(1 mL)+TCL(5×10^(5)个细胞/100μL)、SK-L(1.25 mg/kg)+TCL、SK-M(2.5 mg/kg)+TCL、SK-H(5 mg/kg)+TCL、紫杉醇(PTX 2 mg/kg)+TCL疫苗组。疫苗接种结束后10 d,检测移植瘤体积和小鼠生存率,LDH法检测脾CTL的杀伤能力。结果:SK+TCL刺激的DC疫苗表达出高水平CD80、CD86(均P<0.01);DC与T细胞共培养液上清中IL-12P70、IFN-γ、TNF-α含量均显著上升(均P<0.01),T细胞中T-bet和RORγt的表达显著性上调(均P<0.01)。成功构建Lewis肺癌荷瘤小鼠模型,SK-H+TCL刺激的DC疫苗治疗显著延缓了的移植瘤生长并提高了小鼠的存活率,且可强烈诱导脾CTL的杀伤能力(均P<0.01)。结论:SK+TCL刺激的DC疫苗可激活DC至成熟状态,上调T细胞中T-bet和RORγt的表达,启动Th1效应细胞,SK体内治疗Lewis肺癌荷瘤小鼠具有良好的抗肿瘤效果。

单增李斯特菌溶血素S llsB基因缺失株的构建及部分生物学特性研究

为分析单增李斯特菌溶血素S(listeriolysin S,LLS)llsB基因缺失对单增李斯特菌(Listeria monocytogenes,LM)生物学特性的影响,本研究利用同源重组构建了llsB基因缺失株LM90-ΔllsB,并以8周龄、体重40g±5g的健康昆明系雄性小鼠为试验动物对其生长特性、半数致死量(LD50)、脏器载菌量等生物学特性进行了初步研究。结果显示,本研究成功构建了llsB基因缺失株;缺失株在体外连续培养20代过程中能稳定遗传。通过生长曲线测定发现,llsB基因缺失株生长活性略高于亲本株;小鼠感染试验结果显示,亲本株和缺失株的LD50分别为106.17和106.50 CFU;与亲本株相比,缺失株在小鼠肝脏和脾脏中的载菌量均极显著减少(P<0.01),说明缺失株对小鼠的感染能力明显减弱。在小鼠脑中未检出单增李斯特菌。综上所述,llsB基因对LM90的部分生物学特性可能有直接或间接的调控作用,本试验结果为进一步研究LLS的致病机理及防控李斯特菌病提供一定的理论依据。

抗菌肽LL⁃37在骨质疏松症中的作用机制研究进展

抗菌肽(antimicrobial peptides,AMPS)是一种宿主防御肽,既往主要关注其抗细菌及抗病毒作用,进一步研究发现AMPS可在成骨、破骨、免疫、促进血管生成等多个途径调控骨代谢、参与骨质疏松症的发生发展。本文旨在总结AMPS在骨代谢中的调控机制,探讨其对骨质疏松症的影响为AMPS治疗骨质疏松症提供理论依据。

基于生物信息学方法分析L1CAM在肺鳞状细胞癌中的表达及意义

目的:分析细胞黏附分子Ll基因(L1CAM)在肺鳞状细胞癌中的表达,并探讨其潜在的生物学意义。方法:利用Ualcan数据库分析肺鳞状细胞癌(LUSC)肿瘤组织及正常组织L1CAM表达,并在正常细胞系和肿瘤细胞系中进行实时荧光定量PCR(qPCR)验证;采用Sangerbox3.0分析L1CAM表达与LUSC患者预后的关系;通过LinkedOmics检索L1CAM共表达基因,将获取的基因集进行GO功能富集分析和KEGG通路富集分析;利用STRING数据库构建蛋白相互作用(PPI)网络。结果:与正常肺组织比较,LUSC组织中L1CAM表达水平明显升高;经qPCR实验验证,与正常肺上皮细胞相比,LUSC细胞系中的L1CAM表达量显著升高;L1CAM共表达基因主要富集的生物学过程包括细胞发育、细胞或亚细胞成分的运动、细胞内信号转导等;主要富集的细胞组分包括细胞外基质、内质网腔、线粒体膜等;主要参与的生物学功能包括信号受体结合、钙离子结合、细胞外基质结构成分等;KEGG信号通路主要与黏着斑、ECM受体互作、顺铂耐药性等相关;L1CAM高表达患者的总生存期(OS)、疾病特异性生存期(DSS)、无病间期(DFI)、无进展间期(PFI)明显低于低表达患者,预示高表达L1CAM的不良预后;PPI网络显示L1CAM蛋白与EGFR、NCAM1、ANK1-3等存在互作关系。结论:L1CAM在LUSC组织中高表达,可作为预后指标;该基因的表达参与多种生物学功能,因此有望成为肺鳞癌潜在的生物标志物。

LLS催代酶在啤酒酿造前酵过程中降低双乙酰的研究

在啤酒生产上，成品啤酒中双乙酰（ＶＤＫ）含量对于啤酒的风味有着重要的影响，国际已把此项作为检验啤酒质量的一个重要指标［５］，寻找一种催化α－乙酰乳酸到双乙酰这步反应的催代酶的工作至今仍是国际啤酒行业中的一个重大课题。我们利用ＬＬＳ催代酶，在青岛啤酒一厂的发酵车间进行小规模试验，通过前发酵阶段连续监测，发现加催代酶的试样双乙酰含量出现先高后低的规律，说明催代酶在啤酒的前酵过程中降低双乙酰的作用是明显的。本次实验是国内啤酒行业催代酶的首次研究，通过今后的进一步完善和实验，相信ＬＬＳ催代酶将有广阔的应用前景。

拟南芥突变体lls糖信号响应异常

拟南芥突变体lls(long life span)是生活周期很长的单基因突变体。为了研究该突变对糖信号途径的影响,本文通过形态和生理学分析,结果发现lls突变体具有一些糖相关的表型特征,如:植株矮小,叶色深绿,生长缓慢等。通过测定植株的可溶性糖含量及叶绿素和花青素含量,结果显示lls植株的叶绿素和可溶性糖水平升高,可能由此引起花青素含量升高。施加外源的葡萄糖和蔗糖的实验结果表明lls突变体对外源糖信号敏感,这说明LLS突变可能严重影响了糖代谢或糖响应的某一途径。

LLS催代酶在啤酒生产中的应用研究

就海洋活性物质LLS催代酶的特性及其在啤酒生产中应用机理进行了一定的探讨 ,并对它在啤酒生产中的应用效果做了较为系统的研究 ;特别是LLS催代酶对酵母活性的提高 ,对双乙酰还原的加速、双乙酰前驱体α

LLS培养基的研制及其效果考核

本文采用含量在98.8%的食用赖氨酸和十二烷基硫酸钠代替脱氧胆盐,研制了乳糖赖氨酸十二烷基硫酸钠琼脂培养基(LLS),通过对分离出的168株沙门氏菌的生化试验、肠杆菌科分属噬菌体裂解试验和血清学分型,结果证明LLS培养基分离沙门氏菌的检出率较高(达76.2%),成本低廉(每个平板为0.04元)适于推广使用的一种肠道致病菌分离用的培养基。

Changes of the gastric endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma: An immunohistochemical quantitative study

AIM: The regional distributions and relative frequencies of some gastric endocrine cells of C57BL/6 mice were studied by immunohistochemical method using seven types of specific antisera against chromogranin A (CGA), serotonin, somatostatin, gastrin, cholecystokinin (CCK)-8, glucagon and human pancreatic polypeptide (HPP) after subcutaneous implantation of murine lung carcinoma (3LL) cells. METHODS: The experimental animals were divided into two groups, one is non-implanted sham and the other is 3LL-implanted group. Samples were collected from the two regions of stomach (fundus and pylorus) at 28 d after implantation of 3LL cells (1x105 cell/mouse). RESULTS: In this study, all the seven types of immunoreactive (IR) cells were identified except for HPP. Most of these IR cells in the gastric portion were generally spherical or spindle in shape (open-type cell) while cells showing round in shape (closed-type cell) were found occasionally. The regional distributions of gastric endocrine cells in the 3LL-implanted group were similar to those of non-implanted sham. However, significant decreases of some types of IR cells were detected in 3LL-implanted group compared to those of non-implanted sham. In addition, the IR cells showing degranulation were numerously detected in 3LL-implanted group. CGA-, serotonin- and somatostatin-IR cells in the fundus and pylorus regions, and gastrin-IR cells in the pylorus regions of 3LL-implanted groups significantly decreased compared to those of non-implanted sham. However, no changes on frequencies of CCK-8- and glucagon-IR cells were demonstrated between 3LL-implanted and non-implanted groups. CONCLUSION: Endocrine cells are the anatomical units responsible for the production of gut hormones, and the change in their density would reflect a change in the capacity of producing these hormones. Implantation of tumor cell mass (3LL) induced severe quantitative changes of gastric endocrine cell density, and the abnormality in density of gastric endocrine cells may contribute to the development of gastrointestinal symptoms such as anorexia and indigestion, frequently encountered in patients with cancer.

LLS小汽轮发电机超速风险控制

介绍了秦山第二核电厂1、2号机组的LLS系统的功能、小汽轮机的原理结构及商运期间出现的汽机超速导致LLS发电机损坏事件,并针对试验期间超速导致发电机损坏事件分析了LLS汽轮发电机运行风险,对LLS系统运行提出了改进建议。

岭澳核电站LLS003VV调节原理

LLS系统在岭澳核电站起着非常重要的安全作用,在电站失去全部厂用电源的情况下,该系统能提供9RIS011PO运行所需要的电源,从而给反应堆冷却剂泵的一号轴封供应轴封水,避免了反应堆冷却剂的泄漏,保证了第二道屏障的完整性。此外LLS系统也给NSSS系统、PAMS仪表,主控室应急照明等提供配用电源,以保证一些必要的工况下机组的安全控制。而LLS003VV调节性能的好坏直接关系到LLS系统的正常运行,因此本文着重对LLS003VV的调节原理进行阐述。

Effect of vasoactive intestinal peptide on proliferation of hepatoma cells

It has been found that vasoactive intestinal peptide (VIP) stimulates the growth ofseveral kinds of tumor cells.There is no report so far about the effect of VIP on the growth ofhepatoma cells.Using the tetrazolium colorimetric assay (MTT assay) and cell countingmethod,it was investigated that the effect of VIP on the growth of a cultured rat hepatomaFSK-7902 cells.The results showed that VIP stimulated the proliferation of the rat hepatomacells obviously.The addition of 1μmol/L VIP caused a significant increase in the number of thecultured rat hepatoma cells on 3rd day and maximal increase occured on 4th day and 5th day ( P【0.01).The growth promoting effect was greater as the concentration of VIP increased.Thelowest effective concentration of VIP was 0.5μmol/L.Exposure to VIP for 12 h followed by re-moval of the peptide resulted in sustained growth for several days.

基于地闪回击与LLS的OPGW雷击定位精度分析

OPGW雷击监测作为一种较好的输电线路本体雷击监测手段,其雷击定位准确性无法得到现场验证。文章基于OPGW光纤传感雷击监测装置现场实测的雷击数据,通过与雷电定位系统(LLS)结果数据的横比,以及同一雷击多次地闪回击定位结果的纵比,分析OPGW雷击定位的精度与稳定性;并验证导数模极大值波头标定算法用于OPGW雷击定位的有效性。统计结果表明:OPGW雷击定位与LLS定位结果误差基本保持在1~2基塔以内,且多次地闪回击定位结果基本一致。说明OPGW雷击定位具有较高的现场应用参考价值。

Procedure Design to Fulfill Quality Management System

With the application of the principle of PDCA, and dr awing on the experience of the process methods of the ISO9000 and the system met hods of management, the author analyses the basic requirement to fulfill quality management system in enterprises. Then the author inquires into the procedure t o establish the efficiency quality management system. Finally four stages (plan, do, check, action) and eight steps (decision, plan, analyze, design, do, check, improve, fulfill) are put forward to fulfill quality management system.

Changes of the intestinal endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma (3LL): An immunohistochemical quantitative study

AIM: To study the distributions and frequencies of intestinal endocrine cells in the C57BL/6 mouse with immunohistochemical method using seven types of specific antisera against chromogranin A (CGA), serotonin,somatostatin, glucagons, gastrin, cholecystokinin (CCK)-8 and human pancreatic polypeptide (hPP) after abdominal subcutaneous implantation of murine lung carcinoma (3LL).METHODS: The experimental animals were divided into two groups, one is non-implanted Sham and the other is 3LL-implanted group. Samples were collected from six regions of intestinal tract at 28th d after implantation of 3LL cells (1×105 cell/mouse).RESULTS: In this study, five types of immunoreactive (IR) cells were identified except for gastrin and hPP. The regional distributions of the intestinal endocrine cells in the 3LL-implanted group were similar to those of the non-implanted Sham. However, significant decreases of IR cells were detected in 3LL-implanted group compared to those of non-implanted Sham. CGA- and serotonin-IR cells significantly decreased in 3LL-implanted groups compared to that of non-implanted Sham. Somatostatin-IR cells in the jejunum and ileum and CCK-8-IR cells in the jejunum of 3LL-implanted groups significantly decreased compared to that of non-implanted Sham. In addition,glucagon-IR cells were restricted to the ileum and colon of non-implanted Sham.CONCLUSION: Implantation of tumor cell mass (3LL)induced severe quantifiable changes of intestinal endocrine cell density and the abnormality in density of intestinal endocrine cells may contribute to the development of gastrointestinal symptoms such as anorexia and indigestion, frequently encountered in patients with cancer.

Field measurements for calibration of simplified models of the stiffening effect of infill masonry walls in high-rise RC framed and shear-wall buildings

As a type of nonstructural component, infill walls play a significant role in the seismic behavior of high-rise buildings. However, the stiffness of the infill wall is generally either ignored or considered by simplified empirical criteria that lead to a period shortening. The difference can be greatly decreased by using a structural identification methodology. In this study, an ambient vibration test was performed on four on-site reinforced concrete high-rise buildings, and the design results were compared with the PKPM models using corresponding finite element(FE) models. A diagonal strut model was used to simulate the behavior of the infill wall, and the identified modal parameters measured from the on-site test were employed to calibrate the parameters of the diagonal strut in the FE models. The SAP2000 models with calibrated elastic modulus were used to evaluate the seismic response in the elastic state. Based on the load-displacement relationship of the infill wall, nonlinear dynamic analysis models were built in PERFORM-3 D and calibrated using the measured modal periods. The analysis results revealed that the structural performance under small/large earthquake records were both strengthened by infill walls, and the contribution of infill walls should be considered for better accuracy in the design process.

LLSⅡ培养基的性能考核

ＬＬＳⅡ培养基的性能考核福州铁路卫生防疫站邓涤夷，陈霞，陈毓玲郑州铁路中心卫生防疫站王炜指导何晓青我们采用卫已知的沙门氏菌、弗劳地枸椽酸杆菌、普通变形杆菌、奇异变形杆菌和缓慢爱德华氏菌等来评价ＬＬＳⅡ培养基（１）对离沙门氏菌的效果，现将结果报告如下。...

抗菌肽LL37产生及其对炎症性皮肤病作用的研究进展

抗菌肽LL37是目前临床发现的唯一一种人类Cathelidin家族抗菌肽,其可抵御病原体的侵袭,也能够发挥免疫调节、免疫趋化等作用,从而触发或加剧机体炎症反应进程。玫瑰痤疮和银屑病患者体内高表达的LL37可诱导肥大细胞等炎症细胞分泌多种促炎性细胞因子和趋化因子参与皮损的形成。而特应性皮炎患者的LL37表达减少,使机体皮肤屏障功能受损,更易患细菌感染,因此将LL37作为靶点有望成为玫瑰痤疮等炎症性皮肤病治疗的新思路和新手段。本文对LL37的表达过程和调控机制及其在玫瑰痤疮、银屑病和特应性皮炎患者中发挥的作用和相关机制的研究进展进行综述。