This study develops low-fat microwaved peanut snacks(LMPS)using partially defatted peanuts(PDP)with different defatting ratios,catering to people’s pursuit of healthy,low-fat cuisine.The effects of defatting treatmen...This study develops low-fat microwaved peanut snacks(LMPS)using partially defatted peanuts(PDP)with different defatting ratios,catering to people’s pursuit of healthy,low-fat cuisine.The effects of defatting treatment on the structural characteristics,texture,color,and nutrient composition of LMPS were comprehensively explored.The structural characteristics of LMPS were characterized using X-ray micro-computed tomography(Micro-CT)and scanning electron microscope(SEM).The results demonstrated that the porosity,pore number,pore volume,brightness,brittleness,protein content,and total sugar content of LMPS all significantly increased(P<0.05)with the increase in the defatting ratio.At the micro level,porous structure,cell wall rupture,and loss of intracellular material could be observed in LMPS after defatting treatments.LMPS made from PDP with a defatting ratio of 64.44%had the highest internal pore structural parameters(porosity 59%,pore number 85.3×10^(5),pore volume 68.23 mm3),the brightest color(L^(*) 78.39±0.39),the best brittleness(3.64±0.21)mm^(–1)),and the best nutrition(high protein content,(34.02±0.38)%;high total sugar content,(17.45±0.59)%;low-fat content,(27.58±0.85)%).The study provides a theoretical basis for the quality improvement of LMPS.展开更多
背景与目的:EB病毒编码的潜伏膜蛋白1(latent membrane protein 1,LMP1)在鼻咽癌的浸润和转移过程中起重要的作用。本实验目的在于研究EB病毒LMP1对人鼻咽癌细胞转移能力的影响和探讨其中的相关机制。方法:应用免疫细胞化学RT-PCR法和We...背景与目的:EB病毒编码的潜伏膜蛋白1(latent membrane protein 1,LMP1)在鼻咽癌的浸润和转移过程中起重要的作用。本实验目的在于研究EB病毒LMP1对人鼻咽癌细胞转移能力的影响和探讨其中的相关机制。方法:应用免疫细胞化学RT-PCR法和Western blot检测LMP1、E-cadherin和intercellular adhesion molecule-1 (ICAM-1)在人高分化鼻咽癌细胞系CNE1和转染了LMP1基因的CNE1-GL细胞中的表达。应用细胞-细胞粘附实验、细胞-基质粘附实验、划痕实验和细胞运动实验观察LMP1对鼻咽癌细胞粘附和运动能力的影响。结果:免疫细胞化学结果显示:LMP1在CNE1和CNE1-GL细胞中的阳性率分别为0和(96.60±3.03)%(P<0.01);E-cadherin蛋白的阳性率分别为(37.47±1.50)%和(19.53±1.92)%(P<0.01);ICAM-1蛋白的阳性率分别为(5.27±1.45)%和(93.33±4.23)%(P<0.01)。RT-PCR和Westernblot结果表明,与CNE1细胞相比较,CNE1-GL细胞中E-cadherin的mRNA和蛋白表达明显降低(P<0.01),而ICAM-1的mRNA和蛋白表达则明显升高(P<0.01)。肿瘤细胞同质粘附实验显示,CNE1-GL细胞的同质粘附能力较CNE1细胞弱(P<0.05)。肿瘤细胞-基质粘附实验得出CNE1-GL细胞的异质粘附能力(A值=0.60±0.03)较CNE1细胞(A值=0.46±0.01)强(P<0.01)。肿瘤细胞运动实验显示,穿过PVPF膜的CNE1-GL细胞数多于CNE1细胞(119.3±6.0 vs 46.3±7.0,P<0.05)。结论:LMP1通过抑制E-cadherin表达、促进ICAM-1表达从而抑制肿瘤细胞的同质粘附能力、增强其异质粘附能力和运动能力来参与鼻咽癌的侵袭和转移。展开更多
基金funded by the National Natural Science Foundation of China(NSFC,U21A20270)the Key R&D Program of Shandong Province,China(2023TZXD074)+2 种基金the Bingtuan Science and Technology Program,China(2023AB002)the National Peanut Industry Technology System of China(CARS-13-08B)the National Key R&D Program of China(2021YFD2100402)。
文摘This study develops low-fat microwaved peanut snacks(LMPS)using partially defatted peanuts(PDP)with different defatting ratios,catering to people’s pursuit of healthy,low-fat cuisine.The effects of defatting treatment on the structural characteristics,texture,color,and nutrient composition of LMPS were comprehensively explored.The structural characteristics of LMPS were characterized using X-ray micro-computed tomography(Micro-CT)and scanning electron microscope(SEM).The results demonstrated that the porosity,pore number,pore volume,brightness,brittleness,protein content,and total sugar content of LMPS all significantly increased(P<0.05)with the increase in the defatting ratio.At the micro level,porous structure,cell wall rupture,and loss of intracellular material could be observed in LMPS after defatting treatments.LMPS made from PDP with a defatting ratio of 64.44%had the highest internal pore structural parameters(porosity 59%,pore number 85.3×10^(5),pore volume 68.23 mm3),the brightest color(L^(*) 78.39±0.39),the best brittleness(3.64±0.21)mm^(–1)),and the best nutrition(high protein content,(34.02±0.38)%;high total sugar content,(17.45±0.59)%;low-fat content,(27.58±0.85)%).The study provides a theoretical basis for the quality improvement of LMPS.
文摘背景与目的:EB病毒编码的潜伏膜蛋白1(latent membrane protein 1,LMP1)在鼻咽癌的浸润和转移过程中起重要的作用。本实验目的在于研究EB病毒LMP1对人鼻咽癌细胞转移能力的影响和探讨其中的相关机制。方法:应用免疫细胞化学RT-PCR法和Western blot检测LMP1、E-cadherin和intercellular adhesion molecule-1 (ICAM-1)在人高分化鼻咽癌细胞系CNE1和转染了LMP1基因的CNE1-GL细胞中的表达。应用细胞-细胞粘附实验、细胞-基质粘附实验、划痕实验和细胞运动实验观察LMP1对鼻咽癌细胞粘附和运动能力的影响。结果:免疫细胞化学结果显示:LMP1在CNE1和CNE1-GL细胞中的阳性率分别为0和(96.60±3.03)%(P<0.01);E-cadherin蛋白的阳性率分别为(37.47±1.50)%和(19.53±1.92)%(P<0.01);ICAM-1蛋白的阳性率分别为(5.27±1.45)%和(93.33±4.23)%(P<0.01)。RT-PCR和Westernblot结果表明,与CNE1细胞相比较,CNE1-GL细胞中E-cadherin的mRNA和蛋白表达明显降低(P<0.01),而ICAM-1的mRNA和蛋白表达则明显升高(P<0.01)。肿瘤细胞同质粘附实验显示,CNE1-GL细胞的同质粘附能力较CNE1细胞弱(P<0.05)。肿瘤细胞-基质粘附实验得出CNE1-GL细胞的异质粘附能力(A值=0.60±0.03)较CNE1细胞(A值=0.46±0.01)强(P<0.01)。肿瘤细胞运动实验显示,穿过PVPF膜的CNE1-GL细胞数多于CNE1细胞(119.3±6.0 vs 46.3±7.0,P<0.05)。结论:LMP1通过抑制E-cadherin表达、促进ICAM-1表达从而抑制肿瘤细胞的同质粘附能力、增强其异质粘附能力和运动能力来参与鼻咽癌的侵袭和转移。
