Changes in Metabolites and Allelopathic Effects of Non-Pigmented and Black-Pigmented Lowland Indica Rice Varieties in Phosphorus Deficiency

Phosphorus(P) levels alter the allelopathic activity of rice seedlings against lettuce seeds. In this study, we investigated the effect of P deficiency on the allelopathic potential of non-pigmented and pigmented rice varieties. Rice seedlings of the white variety Khao Dawk Mali(KDML105, non-pigmented) and the black varieties Jao Hom Nin(JHN, pigmented) and Riceberry(RB, pigmented) were cultivated under high P(HP) and low P(LP) conditions. Morphological and metabolic responses to P deficiency were investigated. P deficiency inhibited shoot growth but promoted root growth of rice seedlings in all three varieties. Moreover, P deficiency led to decreased cytosolic phosphate(Pi) and total P concentrations in both shoot and root tissues. The subsequent reduction in internal P concentration enhanced the accumulation of phenolic compounds in both shoot and root tissues of the seedlings. Subsequently, allelopathy-based inter-and intra-specific interactions were assessed using water extracts from seedlings of the three varieties grown under HP and LP conditions. These extracts were tested on seeds of lettuce, the weed Dactyloctenium aegyptium, and the same rice variety. The shoot and root extracts from P-deficient seedlings reduced the germination of all recipient plants. Specifically, the shoot extract from P-deficient KDML105 seedlings reduced the germination index(GI) of lettuce seeds to 1%, while those from P-deficient RB and JHN seedlings produced GIs of 32% and 42%, respectively. However, when rice seeds were exposed to their own LP shoot and root extracts, their GIs increased up to 4-fold, compared with the HP extracts. Additionally, the shoot extracts from P-deficient plants also stimulated the germination of D. aegyptium by about 2–3-fold, whereas the root extracts did not have this effect. Therefore, P starvation led to the accumulation and exudation of phenolics in the shoots and roots of rice seedlings, altering their allelopathic activities. To adapt to P deficiency, rice seedlings potentially release signaling chemicals to suppress nearby competing species while simultaneously promoting their own germination and growth.

QTL Mapping and Q×E Interactions of Grain Cooking and Nutrient Qualities in Rice Under Upland and Lowland Environments

Grain cooking and nutrient qualities are the most important components of rice （Oryza sativa L.） quality. A doubled haploid （DH） population from a cross between two japonica cultivars was used to examine the phenotypic values and potential QTLs for the quality traits. The cooking and nutrient quality traits, including the amylose content （AC）, the gel consistency （CJC）, the gelatinization temperature （GT）, and the protein content （PC）, in rice grown under upland and lowland environments were evaluated. Significant differences for AC, GC, GT, and PC between upland and lowland environments were detected. The phenotypic values of all four traits were higher under upland environment than lowland environment. The value of PC under upland environment was significantly higher （by 37.9%） than that under lowland environment. This suggests that upland cultivation had large effect on both cooking and nutrient qualifies. A total of seven QTLs and twelve pairs of QTLs were detected to have significant additive and epistatic effects for the four traits. Significant Q x E interaction effects of two QTLs and two pairs of QTLs were also discovered. The general contribution of additive QTLs ranged from 1.91% to 19.77%. The Q × E interactions of QTLs QGt3 and QAc6 accounted for 8.99% and 47.86% of the phenotypic variation, respectively, whereas those of the 2 pairs of epistatic QTLs, QAc6-QAcllb and QAc8-QAc9, accounted for 32.54% and 11.82%, respectively. Five QTLs QGt6b, QGt8, QGt11, QGcl, and QPc2, which had relatively high general contribution and no Q x E interactions, were selected to facilitate the upland rice grain quality breeding.

Germplasm Innovation of Heat Tolerance in Rice for Irrigated Lowland Conditions in the Philippines

Heat-tolerant varieties, such as N22 and Dular, which were used in this study, usually have low yield potential and undesirable plant characteristics but combining them with high yielding and improved rice varieties, new heat-tolerant rice genotypes with high yield potential can be achieved. In this study, phenotyping and selecting desirable materials from various crosses were performed under high temperature conditions during the reproductive stage. Screening was performed in the field and glasshouse to select individuals with heat tolerance and high yield potential. Several advanced breeding lines from Gayabyeo/N22 cross produced desirable individuals with heat tolerance, resistance to pests and diseases, and high yield potential. The genetic variation in percent sterility among the selected backcross populations grown in high temperature environments showed that large number of plants can be identified and selected with lower percent sterility.

Genetic Diversity of Rice Landraces from Lowland and Upland Accessions of China

Genetic diversity of rice landraces from lowland and upland accessions of China was investigated using 66 polymorphic simple sequence repeat （SSR） markers. The total number of alleles detected from all 324 tested accessions was 555 with an average allele number （Na） of 8.409 per locus, the average effective number of alleles （Ne） of 3.574 and the average Shannon＇s information index （I） of 1.378. The genetic diversity was higher for the indica landraces compared to the japonica landraces, and the upland landraces were more genetically diverse than the lowland landraces. The SSR markers, RM72, RM232, RM219, RM241, RM224 and RM3 showed the highest rates of polymorphism and these SSR markers were suitable to assess the genetic diversity of rice germplasm resources. A dendrogram of 324 accessions of lowland and upland landraces showed that all rice accessions were mainly subdivided into two groups, japonica and indica, with some being intermediate. The distribution of lowland and upland landraces among the japonica and indica rice groups was distinct, with obvious differentiation between the lowland and upland landraces in japonica rice, but no such clear distinction in indica rice.

RICE原则联合手法淋巴引流技术治疗急性期踝关节扭伤的临床效果

踝关节扭伤的急性期,在RICE原则下,结合应用优势较高、疗效突出的手法淋巴结引流技术,探究其应用效果和干预积极性。方法 纳入2020.01-2024.01期间治疗的80例踝关节扭伤患者,均属于急性期,按照随机数字表法,将其分为对比组、试验组,各40例,分别采取常规(RICE原则)、常规+手法淋巴引流技术,对比差异。结果 试验组的疗效更低(P<0.05);干预后,试验组的疼痛、肿胀和踝关节功能评分均更好(P<0.05),其肢体周径、细胞外液含量指标均更好(P<0.05)。结论 联合干预模式(RICE+手法淋巴引流技术)可以有效减少踝关节损伤程度,也可有效减少肿胀程度,减少肢体周径,减少细胞外液浓度,可以有效减少疼痛感,缩短病程,加快疾病康复速度,其干预优势明显。

Effects of Farmyard Manure and Inorganic Fertilizer Application on Soil Physico-Chemical Properties and Nutrient Balance in Rain-Fed Lowland Rice Ecosystem

A field experiment was conducted to assess the effects of combined application of farm yard manure (FYM) and inorganic NP fertilizers on soil physico-chemical properties and nutrient balance in a rain-fed lowland rice production system in Fogera plain, northwestern Ethiopia. The study was carried out during the main cropping seasons of 2010 and 2011. Twenty-seven treatments comprising a factorial combination of three rates of FYM (0, 7.5, and 15 t·ha-1), three rates of nitrogen (0, 60, 120 kg·N·ha-1) and three rates of phosphorus (0, 50 and 100 kg·P2O5·ha-1) were tested. The experiments were laid out as a randomized complete block design with three replications. Bulk density, organic matter content, and available water holding capacity, total N, and available P of the soil were measured just after harvesting the rice crop. Results showed that application of 15 t·FYM·ha-1 significantly increased soil organic matter and available water holding capacity but decreased the soil bulk density, creating a good soil condition for enhanced growth of the rice crop. Application of 15 tFYM·ha-1 increased the level of soil total nitrogen from 0.203% to 0.349%. Combined application of 15 t·ha-1·FYM and 100 kg·P2O5·ha-1 increased the available phosphorous from 11.9 ppm to 38.1 ppm. Positive balances of soil N and P resulted from combined application of FYM and inorganic N and P sources. Application of 15·t ha-1·FYM and 120 kg·N·ha-1resulted in 214.8 kg·ha-1·N positive balance while application of 15 t·ha-1·FYM and 100 kg·P2O5·ha-1 resulted in a positive balance of 69.3 kg·P2O5·ha-1 available P. From the results of this experiment, it could be concluded that combined application of FYM and inorganic N and P fertilizers improved the chemical and physical properties, which may lead to enhanced and sustainable production of rice in the study area.

Effect of Phosphorus and Irrigation Levels on Yield,Water Productivity,Phosphorus Use Efficiency and Income of Lowland Rice in Northwest Pakistan

With decreasing availability of water for agriculture and increasing demand for rice production, an optimum use of irrigation water and phosphorus may guarantee sustainable rice production. Field experiments were conducted in 2003 and 2004 to investigate the effect of phosphorus and irrigation levels on yield, water productivity （WP）, phosphorus use efficiency （PUE） and income of low land rice. The experiment was laid out in randomized complete block design with split plot arrangements replicated four times. Main plot consisted of five phosphorus levels, viz. 0 （P0）, 50 （P50）, 100 （P100）, 150 （P15o）, and 200 （P200） kg/hm2, while subplots contained of irrigation times, i.e. 8 （I8）, 10 （I10）, 12 （I12）, and 14 （I14） irrigation levels, each with a water depth of 7.5 cm. Mean values revealed that P150 in combination with I10 produced the highest paddy yield （9.8 t/hm2） and net benefit （1 231.8 US$/hm2） among all the treatments. Phosphorus enhanced WP when applied in appropriate combination with irrigation level. The highest mean WP [13.3 kg/（hm2-mm）] could be achieved at Plso with 18 and decreased with increase in irrigation level, while the highest mean PUE （20.1 kg/kg） could be achieved at P100 with I10 and diminished with higher P levels. The overall results indicate that P150 along with I10 was the best combination for sustainable rice cultivation in silty clay soil.

Upland rice and lowland rice exhibited different P/P expression under water deficit and ABA treatment

Aquaporins play a significant role in plant water relations. To further understand the aquaporin function in plants under water stress, the expression of a subgroup of aquaporins, plasma membrane intrinsic proteins （PIPs）, was studied at both the protein and mRNA level in upland rice （Oryza sativa L. cv. Zhonghan 3） and lowland rice （Oryza sativa L. cv. Xiushui 63） when they were water stressed by treatment with 20% polyethylene glycol （PEG）. Plants responded differently to 20% PEG treatment. Leaf water content of upland rice leaves was reduced rapidly. PIP protein level increased markedly in roots of both types, but only in leaves of upland rice after 10 h of PEG treatment. At the mRNA level, OsPIP1,2, OsPIP1,3, OsPIP2;1 and OsPIP2;5 in roots as well as OsPIP1,2 and OsPIP1;3 in leaves were significantly up-regulated in upland rice, whereas the corresponding genes remained unchanged or down-regulated in lowland rice. Meanwhile, we observed a significant increase in the endogenous abscisic acid （ABA） level in upland rice but not in lowland rice under water deficit. Treatment with 60 μM ABA enhanced the expression of OsPIP1;2, OsPIP2;5 and OsPIP2;6 in roots and OsPIP1;2, OsPIP2;4 and OsPIP2;6 in leaves of upland rice. The responsiveness of PIP genes to water stress and ABA were different, implying that the regulation of PIP genes involves both ABA-dependent and ABA-independent signaling oathways during water deficit.

Leaf area index based nitrogen diagnosis in irrigated lowland rice

Leaf area index （LAI） is used for crop growth monitoring in agronomic research, and is promising to diagnose the nitrogen （N） status of crops. This study was conducted to develop appropriate LAI-based N diagnostic models in irrigated lowland rice. Four field experiments were carried out in Jiangsu Province of East China from 2009 to 2014. Different N application rates and plant densities were used to generate contrasting conditions of N availability or population densities in rice. LAI was determined by LI-3000, and estimated indirectly by LAI-2000 during vegetative growth period. Group and individual plant characters （e.g., tiller number （TN） and plant height （H）） were investigated simultaneously. Two N indicators of plant N accumulation （NA） and N nutrition index （NNI） were measured as well. A calibration equation （LAI=1.7787LAI2o00-0.8816, R2=0.870＂） was developed for LAI-2000. The linear regression analysis showed a significant relationship between NA and actual LAI （R2=0.863^＊＊）. For the NNI, the relative LAI （R2=0.808-） was a relatively unbiased variable in the regression than the LAI （R^2=0.33^＊＊）. The results were used to formulate two LAI-based N diagnostic models for irrigated lowland rice （NA=29.778LAI-5.9397; NNI=0.7705RLAI＋0.2764）. Finally, a simple LAI deterministic model was developed to estimate the actual LAI using the characters of TN and H （LAI=-0.3375（THxHx0.01）2＋3.665（TH×H×0.01）-1.8249, R2=0.875＊＊）. With these models, the N status of rice can be diagnosed conveniently in the field.

Assessment of Variation in Morpho-Physiological Traits and Genetic Diversity in Relation to Submergence Tolerance of Five Indigenous Lowland Rice Landraces

The present study evaluated submergence responses in 88 lowland indigenous rice(Oryza sativa L.) landraces from Koraput, India, to identify submergence-tolerant rice genotypes. In pot experiments, variations in survival rate, shoot elongation, relative growth index, dry matter, chlorophyll, soluble sugar and starch contents were evaluated in two consecutive years under well-drained and completely submerged conditions. Principal component analysis showed that the first three axes contributed 96.820% of the total variation among the landraces, indicating wide variation between genotypes. Major traits such as survival rate, relative growth index, soluble sugar and starch contents appeared to be important determinants of phenotypic diversity among the landraces. Phenotypic coefficient of variance was higher than genotypic coefficient of variance for all the traits and all showed high heritability(90.38%–99.54%). Five rice landraces(Samudrabali, Basnamundi, Gadaba, Surudaka and Dokarakuji) were the most tolerant to submergence. When submerged for up to 14d, Samudrabali, Basnamundi and Godoba were notable for having greater survival rates than a standard submergence tolerant variety FR13 A, and also notable for elongating more vigorously and accumulating more biomass. These three landraces may therefore be especially useful in lowland rice growing areas that are affected by both moderate stagnant water and flash flooding. Molecular genotyping revealed that the submergence tolerance of Samudrabali, Basnamundi and Godoba is linked to the presence of one or more different Sub1 loci and it may well prove useful for breeding improved submergence tolerant rice varieties, thereby assising to improve yield stability in the rainfed lowland agro-ecosystem.

Growth and Physiological Performance of Aerobic and Lowland Rice as Affected by Water Stress at Selected Growth Stages

Aerobic rice technology is still new in Malaysia, and information regarding MARDI Aerob 1(MA1), the first local aerobic rice variety, is still lacking. Therefore, comparative studies were carried out to determine the physiological performance of aerobic rice variety MA1 and lowland rice variety MR253 under water stress given at the panicle initiation, flowering and ripening stages. This experiment was arranged in a randomized complete block design. Stomatal conductance(g_s), chlorophyll a fluorescence(F_v/F_m), leaf relative water content(leaf RWC), and soil moisture content(SMC) as well as yield component parameters such as panicle number, grain yield and 100-grain weight were measured. Results revealed that gs and leaf RWC for both varieties decreased with depletion of SMC. The correlation study between the physiological parameters and SMC indicated that F_v/F_m was not affected by water stress, regardless of varieties. The yield components(panicle number, grain yield and 100-grain weight) for both varieties greatly decreased when water stress was imposed at the panicle initiation stage. This study showed that the panicle initiation period was the most sensitive stage to water stress that contributed to a substantial reduction in yield for both varieties. Under the aerobic condition(control), MR253 produced higher panicle number, 100-grain weight and yield than MA1. Although MR253 is bred for lowland, it is well adapted to aerobic condition.

The differenee in protein content,MDH,POD and COD isozymes during root different developmental stages in lowland rice and upland rice

The difference in protein content, MDH,POD and COD isozymes have been seenbetween lowland rice roots and upland rice roots during root growth and development. High pro-tein content has been examined in upland rice roots,but isozyme bands in lowland rice roots aremore than that in upland rice roots.Total area of POD isozyme bands at seedling and tilleringstages of upland rice is larger than that of lowland rice.The POD isozymes zymogram in the rootof upland rice is more stable compared with that of lowland rice.More COD isozyme numbershave been showed in roots of upland rice,but the relative activity of COD isozyme in roots of up-land rice at the seedling and tillering stages is lower than that of lowland rice.COD7 band can beshown in upland rice root at flowering stage,but it is absent in lowland rice.Therefore,COD7,band can be taken as an index for resistance of the upland rice to drought environment.

Assessing Suitability of Irrigation Scheduling Decision Support Systems for Lowland Rice Farmers in Sub-Saharan Africa—A Review

Irrigation in lowland rice production systems in Sub-Saharan Africa (SSA) is mainly based on traditional surface irrigation methods with continuous flooding practices. This irrigation method ends up using a lot more water that would have otherwise been used to open more land and be used in other water-requiring sectors. Various studies suggest Alternate Wetting and Drying (AWD) as an alternative practice for water management that reduces water use without significantly affecting yield. However, this practice has not been well adopted by the farmers despite its significant benefits of reduced total water use. Improving the adoption of AWD using irrigation Decision Support Systems (DSSs) helps the farmer on two fronts;to know “how much water to apply” and “when to irrigate”, which is very critical in maximizing productivity. This paper reviews the applicability of DSSs using AWD in lowland rice production systems in Sub-Saharan Africa.

Integrated management of African rice gall midge (AfRGM) (<i>Orseolia oryzivora,</i>Harris and gagné), weeds and diseases of lowland rice

Studies were conducted during the 2001 and 2002 cropping years at Edozhigi and Sachi in the Southern Guinea Savannah of Nigeria to study the effect of following rice with another crop on the next rice crop. There were twelve treatments consisting of poor, good and excellent weed management and pesticidal applications against (AfRGM and diseases set up in a randomized complete block design, in twelve plots in 3 replicates. Results showed that rice treated with gramular isazofos at 0.75 kg a.i. ha–1 and benomyl at 1.0 kg/ha at 20 and 40 days after transplanting (DAT), with good weed management and a follow up crop after rice reduced AfRGM occurrence and disease incidence with a consequent increase in yield of the next rice crop. In 2001 infestation of rice by ARGM was more at Sachi than Edozhigi, while in 2002 there was low ARGM occurrence at the two locations. Brown leaf spot was high at the two locations in the two years of the trial while leaf blast was checked by the integrated control methods used in the study. Higher yields were (1.7 - 11.9 t·ha) from treated-well weeded plots that were cropped to cowpea after the 2001 rice harvest Ludwigia decurens, Sphanoclea geylanica Cyperus difformis and Lepllocidia chinensis were the widely occurring weed species at Edozhigi site while Cyperus difformis, Ipomea mvolucrata, F. awuata and Firnbristylis litoralis were weed species prevalent at Sachi site. The rice nematode, hirschmanniella sp. was recorded at the Sachi site along with other nematodes but was absent at the Edozhigi site. The net benefit farmers would derive for following this rice-dry-season crop sequence vary from US$ 172 - 427 and US$ 175 - 265 ha–1 at Sachi and Edozhigi respectively.

Effects of puddling on percolation and rice yields in rainfed lowland paddy cultivation: Case study in Khammouane province, central Laos

We investigated the effects of puddling on percolation and rice yields in rainfed lowland paddy cultivation. We selected a study village in Khammouane province, central Laos, and set up non-puddling and puddling plots from high to low positions. Even when puddling was conducted carefully, the ponding water in the plots disappeared in the case of little rainfall. Further, percolatifons during the later periods of rice growth increased drastically. Therefore, it is difficult to overcome drought stresses only by conducting puddling. We also compared the water conditions in the non-puddling and puddling plots. In the puddling plots at high position and low position along a stream, the number of days without ponding water in the puddling plots was less than that in the non-puddling field in July, suggesting the possibility of a different transplanting date. We tried to estimate the effects of transplanting date on the rice yields and found that transplanting 15 days earlier leads to an increase of 0.5 t/ha in the rice yields. Moreover, the profits from the increased yields exceed the puddling costs considerably, leading to a definite increase in income. Because the transplanting date has no effects in the fields with high ground water, puddling is effective in paddy fields where ponding does not occur to a significant degree.

Assessing Silicon Availability in Soils of Rice-Growing Lowlands and Neighboring Uplands in Benin and Nigeria

Silicon(Si) is known as a beneficial nutrient in the cultivation of rice, playing a key role in photosynthesis enhancement, lodging resistance and tolerance to various environmental stress. The present study aimed to examine available Si content in both lowland soils(n = 29) and neighboring upland soils(n = 21) collected from Benin and Nigeria and to evaluate the validity of the assessment results through a pot experiment. Our results revealed that the acetate-buffer method predicted Si concentration in rice straw at the harvest stage(R^2 = 0.68, P < 0.01) better than the anaerobic-incubation method(R2 = 0.31, P > 0.05), and 76% of the uplands and 38% of the lowlands were deficient(< 50 mg/kg) in acetate-buffer soluble Si. These findings suggest that the Si-deficiency soils prevail across the study area, making rice plants starved for Si and prone to environmental stress.

Impact of Smart Valley on Soil Moisture Content and Rice Yield in Some Lowlands in Burkina Faso

To reduce the impact of rainfall variability on lowland rice yields, Burkina Faso state develops lowlands for small rice farmers. However, the high cost of these infrastructures makes impossible to duplicate them to satisfy the needs which are enormous. The Smart-Valley technology which is actually popularized in certain coastal countries of West Africa would therefore be a boon to increase the productivity of the Sudanese lowlands if it well regulates runoff. The object of this study was therefore to know if smart valley technology could increase soil moisture in order to mitigate the impact of drought’s pockets on rice cultivation in the Sudanese lowlands. The experiment takes place in three lowlands during the rainy seasons 2018 and 2019. The climatic data comes from the meteorological stations in the study areas as well as those installed on the sites. The infiltration measurements were carried out using the double Muntz ring. The soil moisture measurement device consisted of a smart valley area of 5 ha and an undeveloped area of 5 ha per site. Sixteen tubes were installed per lowland allowing the humidity to be measured at a depth of 10, 20, 30, 40 cm using a probe. Four rice varieties, Orylux6, FKR62N, FKR19 and FKR64 were tested on plots of 0.25 ha per variety in the smart valley and undeveloped parts. The results showed that the humidity level was 12% higher in the smart-valley plots throughout the cycle compared to the unmanaged area. In addition, humidity decreases rapidly in unmanaged plots as rain becomes increasingly scarce. Finally, the smart-valley development allowed an average increase in rice yields of 21% compared to the average yield of undeveloped plots.

The underlying mechanism of variety–water–nitrogen–stubble damage interactions on yield formation in ratoon rice with low stubble height under mechanized harvesting

Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary bud sprouting and yield formation in ratoon rice. This study used widely recommended conventional rice Jiafuzhan and hybrid rice Yongyou 2640 as the test materials to conduct a four-factor block design field experiment in a greenhouse of the experimental farm of Fujian Agricultural and Forestry University, China from 2018 to 2019.The treatments included fertilization and no fertilization, alternate wetting and drying irrigation and continuous water flooding irrigation, and plots with and without artificial crushing damage on the rice stubble. At the same time, a 13C stable isotope in-situ detection technology was used to fertilize the pot experiment. The results showed significant interactions among varieties, water management, nitrogen application and stubble status.Relative to the long-term water flooding treatment, the treatment with sequential application of nitrogen fertilizer coupled with moderate field drought for root-vigor and tiller promotion before and after harvesting of the main crop, significantly improved the effective tillers from low position nodes. This in turn increased the effective panicles per plant and grains per panicle by reducing the influence of artificial crushing damage on rice stubble and achieving a high yield of the regenerated rice. Furthermore, the partitioning of 13C assimilates to the residual stubble and its axillary buds were significantly improved at the mature stage of the main crop, while the translocation rate to roots and rhizosphere soil was reduced at the later growth stage of ratooning season rice. This was triggered by the metabolism of hormones and polyamines at the stem base regulated by the interaction of water and fertilizer at this time. We therefore suggest that to achieve a high yield of ratoon rice with low stubble height under mechanized harvesting, the timely application of nitrogen fertilizer is fundamental,coupled with moderate field drying for root-vigor preservation and tiller promotion before and after the mechanical harvesting of the main crop.

A phenology-based vegetation index for improving ratoon rice mapping using harmonized Landsat and Sentinel-2 data

Ratoon rice,which refers to a second harvest of rice obtained from the regenerated tillers originating from the stubble of the first harvested crop,plays an important role in both food security and agroecology while requiring minimal agricultural inputs.However,accurately identifying ratoon rice crops is challenging due to the similarity of its spectral features with other rice cropping systems(e.g.,double rice).Moreover,images with a high spatiotemporal resolution are essential since ratoon rice is generally cultivated in fragmented croplands within regions that frequently exhibit cloudy and rainy weather.In this study,taking Qichun County in Hubei Province,China as an example,we developed a new phenology-based ratoon rice vegetation index(PRVI)for the purpose of ratoon rice mapping at a 30 m spatial resolution using a robust time series generated from Harmonized Landsat and Sentinel-2(HLS)images.The PRVI that incorporated the red,near-infrared,and shortwave infrared 1 bands was developed based on the analysis of spectro-phenological separability and feature selection.Based on actual field samples,the performance of the PRVI for ratoon rice mapping was carefully evaluated by comparing it to several vegetation indices,including normalized difference vegetation index(NDVI),enhanced vegetation index(EVI)and land surface water index(LSWI).The results suggested that the PRVI could sufficiently capture the specific characteristics of ratoon rice,leading to a favorable separability between ratoon rice and other land cover types.Furthermore,the PRVI showed the best performance for identifying ratoon rice in the phenological phases characterized by grain filling and harvesting to tillering of the ratoon crop(GHS-TS2),indicating that only several images are required to obtain an accurate ratoon rice map.Finally,the PRVI performed better than NDVI,EVI,LSWI and their combination at the GHS-TS2 stages,with producer's accuracy and user's accuracy of 92.22 and 89.30%,respectively.These results demonstrate that the proposed PRVI based on HLS data can effectively identify ratoon rice in fragmented croplands at crucial phenological stages,which is promising for identifying the earliest timing of ratoon rice planting and can provide a fundamental dataset for crop management activities.

Regulation of 2-acetyl-1-pyrroline and grain quality in early-season indica fragrant rice by nitrogen and silicon fertilization under different plantation methods

Fragrant rice has a high market value,and it is a popular rice type among consumers owing to its pleasant flavor.Plantation methods,nitrogen(N)fertilizers,and silicon(Si)fertilizers can affect the grain yield and fragrance of fragrant rice.However,the core commercial rice production attributes,namely the head rice yield(HRY)and 2-acetyl-1-pyrroline(2-AP)content of fragrant rice,under various nitrogen and silicon(N-Si)fertilization levels and different plantation methods remain unknown.The field experiment in this study was performed in the early seasons of 2018 and 2019 with two popular indica fragrant rice cultivars(Yuxiangyouzhan and Xiangyaxiangzhan).They were grown under six N-Si fertilization treatments(combinations of two levels of Si fertilizer,0 kg Si ha^(−1)(Si0)and 150 kg Si ha^(−1)(Si1),and three levels of N fertilizer,0 kg N ha^(−1)(N0),150 kg N ha^(−1)(N1),and 220 kg N ha^(−1)(N2))and three plantation methods(artificial transplanting(AT),mechanical transplanting(MT),and mechanical direct-seeding(MD)).The results showed that the N-Si fertilization treatments and all the plantation methods significantly affected the HRY and 2-AP content and related parameters of the two different fragrant rice cultivars.Compared with the Si0N0 treatment,the N-Si fertilization treatments resulted in higher HRY and 2-AP contents.The rates of brown rice,milled rice,head rice,and chalky rice of the fragrant rice also improved with the N-Si fertilization treatments.The N-Si fertilization treatments increased the activities of N metabolism enzymes and the accumulation of N and Si in various parts of the fragrant rice,and affected their antioxidant response parameters.The key parameters for the HRY and 2-AP content were assessed by redundancy analysis.Furthermore,the structural equation model revealed that the Si and N accumulation levels indirectly affected the HRY by affecting the N metabolism enzyme activity,N use efficiency,and grain quality of fragrant rice.Moreover,high N and Si accumulation directly promoted the 2-AP content or affected the antioxidant response parameters and indirectly regulated 2-AP synthesis.The interactions of the MT method with the N-Si fertilization treatments varied in the fragrant rice cultivars in terms of the HRY and 2-AP content,whereas the MD method was beneficial to the 2-AP content in both fragrant rice cultivars under the N-Si fertilization treatments.