Global Navigation Satellite System(GNSS)-based passive radar(GBPR)has been widely used in remote sensing applications.However,for moving target detection(MTD),the quadratic phase error(QPE)introduced by the non-cooper...Global Navigation Satellite System(GNSS)-based passive radar(GBPR)has been widely used in remote sensing applications.However,for moving target detection(MTD),the quadratic phase error(QPE)introduced by the non-cooperative target motion is usually difficult to be compensated,as the low power level of the GBPR echo signal renders the estimation of the Doppler rate less effective.Consequently,the moving target in GBPR image is usually defocused,which aggravates the difficulty of target detection even further.In this paper,a spawning particle filter(SPF)is proposed for defocused MTD.Firstly,the measurement model and the likelihood ratio function(LRF)of the defocused point-like target image are deduced.Then,a spawning particle set is generated for subsequent target detection,with reference to traditional particles in particle filter(PF)as their parent.After that,based on the PF estimator,the SPF algorithm and its sequential Monte Carlo(SMC)implementation are proposed with a novel amplitude estimation method to decrease the target state dimension.Finally,the effectiveness of the proposed SPF is demonstrated by numerical simulations and pre-liminary experimental results,showing that the target range and Doppler can be estimated accurately.展开更多
【目的】制备抗Luman募集因子N端(Luman-recruiting factor N terminal,LRF-N)蛋白的单克隆抗体。【方法】PCR扩增LRF-N端的194bp序列,构建LRF-N重组质粒pGEX-LRF-N,转染BL21(DE3)菌,诱导表达并纯化GST-LRF-N融合蛋白,以其作为抗原免疫6...【目的】制备抗Luman募集因子N端(Luman-recruiting factor N terminal,LRF-N)蛋白的单克隆抗体。【方法】PCR扩增LRF-N端的194bp序列,构建LRF-N重组质粒pGEX-LRF-N,转染BL21(DE3)菌,诱导表达并纯化GST-LRF-N融合蛋白,以其作为抗原免疫6～8周龄雌性BALB/c小鼠,免疫4次,每次间隔10d,第4次加强免疫3d后采取小鼠脾脏,分离脾细胞,与SP2/0细胞融合,用ELISA和Western blot进行阳性细胞株的筛选和鉴定,对获得的杂交瘤细胞的细胞核型、分泌稳定性以及所分泌的单克隆抗体进行鉴定。【结果】pGEX-LRF-N在大肠杆菌BL21(DE3)中得到高效表达,GST-LRF-N蛋白分子质量约为33ku,与预期结果一致。试验共获得3株稳定分泌抗LRF-N抗体的杂交瘤细胞株,分别命名为2AC9、2AG10和3G7。单抗2AC9、2AG10、3G7能够特异性地识别GST-LRF-N蛋白,亚型鉴定结果显示,其重链分别为IgG2a、IgM和IgG1。【结论】获得的抗LRF-N抗体的杂交瘤细胞株2AC9、2AG10、3G7单抗特异性良好,能够稳定分泌抗体,可用于小鼠LRF蛋白生物学功能及活性的进一步研究。展开更多
The long-range forecasts (LRF) based on statistical methods for southwest monsoon rainfall over India (ISMR) has been issued by the India Meteorological Department (IMD) for more than 100 years. Many statistical and d...The long-range forecasts (LRF) based on statistical methods for southwest monsoon rainfall over India (ISMR) has been issued by the India Meteorological Department (IMD) for more than 100 years. Many statistical and dynamical models including the operational models of IMD failed to predict the operational models of IMD failed to predict the deficient monsoon years 2002 and 2004 on the earlier occasions and so had happened for monsoon 2009. In this paper a brief of the recent methods being followed for LRF that is 8-parameter and 10-parameter power regression models used from 2003 to 2006 and new statistical ensemble forecasting system are explained. Then the new three stage procedure is explained. In this the most pertinent predictors are selected from the set of all the potential predictors for April, June and July models. The model equations are developed by using the linear regression and neural network techniques based upon training set of the 43 years of data from 1958 to 2000. The skill of the models is evaluated based upon the validation set of 11 years of data from 2001 to 2011, which has shown the high skill on the validation data set. It can be inferred that these models have the potential to provide a prediction of ISMR, which would significantly improve the operational forecast.展开更多
In this paper we present a generalized lattice Boltzmann based approach for sliding-mesh local reference frame.This scheme exactly conserves hydrodynamic fluxes across local reference frame interface.The accuracy and ...In this paper we present a generalized lattice Boltzmann based approach for sliding-mesh local reference frame.This scheme exactly conserves hydrodynamic fluxes across local reference frame interface.The accuracy and robustness of our scheme are demonstrated by benchmark validations.展开更多
Langham Hotels Upgrade The Langham Hotel has received several accolades and awards recently, including being named in"Travel+ Leisure’s 500 Best Hotels in the World"and ranking among the list of"Travel...Langham Hotels Upgrade The Langham Hotel has received several accolades and awards recently, including being named in"Travel+ Leisure’s 500 Best Hotels in the World"and ranking among the list of"Travel+ Leisure’s 50 Best Hotels in Asia."Langham Hotels is a dynamic,modem hospitality management company which operates a number of luxury hotels and serviced apartment properties in prime loca- tions within Hong Kong and overseas. The hotel has invested＄2.2 million in renovating hotel rooms.The renovation project is led by Andy Tait,a designer from the professional designing company LRF Design,with the first phase of renovationt to be finished by this October.展开更多
基金supported by the National Natural Science Foundation of China(62101014)the National Key Laboratory of Science and Technology on Space Microwave(6142411203307).
文摘Global Navigation Satellite System(GNSS)-based passive radar(GBPR)has been widely used in remote sensing applications.However,for moving target detection(MTD),the quadratic phase error(QPE)introduced by the non-cooperative target motion is usually difficult to be compensated,as the low power level of the GBPR echo signal renders the estimation of the Doppler rate less effective.Consequently,the moving target in GBPR image is usually defocused,which aggravates the difficulty of target detection even further.In this paper,a spawning particle filter(SPF)is proposed for defocused MTD.Firstly,the measurement model and the likelihood ratio function(LRF)of the defocused point-like target image are deduced.Then,a spawning particle set is generated for subsequent target detection,with reference to traditional particles in particle filter(PF)as their parent.After that,based on the PF estimator,the SPF algorithm and its sequential Monte Carlo(SMC)implementation are proposed with a novel amplitude estimation method to decrease the target state dimension.Finally,the effectiveness of the proposed SPF is demonstrated by numerical simulations and pre-liminary experimental results,showing that the target range and Doppler can be estimated accurately.
文摘【目的】制备抗Luman募集因子N端(Luman-recruiting factor N terminal,LRF-N)蛋白的单克隆抗体。【方法】PCR扩增LRF-N端的194bp序列,构建LRF-N重组质粒pGEX-LRF-N,转染BL21(DE3)菌,诱导表达并纯化GST-LRF-N融合蛋白,以其作为抗原免疫6～8周龄雌性BALB/c小鼠,免疫4次,每次间隔10d,第4次加强免疫3d后采取小鼠脾脏,分离脾细胞,与SP2/0细胞融合,用ELISA和Western blot进行阳性细胞株的筛选和鉴定,对获得的杂交瘤细胞的细胞核型、分泌稳定性以及所分泌的单克隆抗体进行鉴定。【结果】pGEX-LRF-N在大肠杆菌BL21(DE3)中得到高效表达,GST-LRF-N蛋白分子质量约为33ku,与预期结果一致。试验共获得3株稳定分泌抗LRF-N抗体的杂交瘤细胞株,分别命名为2AC9、2AG10和3G7。单抗2AC9、2AG10、3G7能够特异性地识别GST-LRF-N蛋白,亚型鉴定结果显示,其重链分别为IgG2a、IgM和IgG1。【结论】获得的抗LRF-N抗体的杂交瘤细胞株2AC9、2AG10、3G7单抗特异性良好,能够稳定分泌抗体,可用于小鼠LRF蛋白生物学功能及活性的进一步研究。
文摘The long-range forecasts (LRF) based on statistical methods for southwest monsoon rainfall over India (ISMR) has been issued by the India Meteorological Department (IMD) for more than 100 years. Many statistical and dynamical models including the operational models of IMD failed to predict the operational models of IMD failed to predict the deficient monsoon years 2002 and 2004 on the earlier occasions and so had happened for monsoon 2009. In this paper a brief of the recent methods being followed for LRF that is 8-parameter and 10-parameter power regression models used from 2003 to 2006 and new statistical ensemble forecasting system are explained. Then the new three stage procedure is explained. In this the most pertinent predictors are selected from the set of all the potential predictors for April, June and July models. The model equations are developed by using the linear regression and neural network techniques based upon training set of the 43 years of data from 1958 to 2000. The skill of the models is evaluated based upon the validation set of 11 years of data from 2001 to 2011, which has shown the high skill on the validation data set. It can be inferred that these models have the potential to provide a prediction of ISMR, which would significantly improve the operational forecast.
文摘In this paper we present a generalized lattice Boltzmann based approach for sliding-mesh local reference frame.This scheme exactly conserves hydrodynamic fluxes across local reference frame interface.The accuracy and robustness of our scheme are demonstrated by benchmark validations.
文摘Langham Hotels Upgrade The Langham Hotel has received several accolades and awards recently, including being named in"Travel+ Leisure’s 500 Best Hotels in the World"and ranking among the list of"Travel+ Leisure’s 50 Best Hotels in Asia."Langham Hotels is a dynamic,modem hospitality management company which operates a number of luxury hotels and serviced apartment properties in prime loca- tions within Hong Kong and overseas. The hotel has invested＄2.2 million in renovating hotel rooms.The renovation project is led by Andy Tait,a designer from the professional designing company LRF Design,with the first phase of renovationt to be finished by this October.
