[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the c...[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the collected 52 morel samples were sequenced,and BLAST alignment was performed by NCBI to construct phylogenetic trees.[Results]The collected morel strains were mainly divided into two major groups:namely black morel and yellow morel groups,of which the black morel group was most,mainly including Morchella sextelata,Morchella importuna,Morchella crassipes,and Morchella eximia.There were very few yellow morel strains,including M.crassipes and M.eximia.[Conclusions]This study lays a foundation for the classification and research of morel germplasm resources,and provides a powerful reference value for the research and production of high-quality morel strains.展开更多
高蛋白的抗病马铃薯对发展中国家和美国均有价值。路易丝安那州立大学(LSU)Jes- se Jaynes博士已合成编码含大量必需氨基酸的蛋白的基因,该蛋白“比现存任何蛋白都好”。该氏合成该基因后,使之在大肠杆菌中表达,然后尝试将其引入各种植...高蛋白的抗病马铃薯对发展中国家和美国均有价值。路易丝安那州立大学(LSU)Jes- se Jaynes博士已合成编码含大量必需氨基酸的蛋白的基因,该蛋白“比现存任何蛋白都好”。该氏合成该基因后,使之在大肠杆菌中表达,然后尝试将其引入各种植物——马铃薯是第一种。该基因在马铃薯中获表达,并已检测出。展开更多
Chaetoceros Ehrenberg is one of the most diverse genera of planktonic diatoms.The species in section Chaetoceros are characterized by cells and setae having numerous chloroplasts and being widely distributed.However,t...Chaetoceros Ehrenberg is one of the most diverse genera of planktonic diatoms.The species in section Chaetoceros are characterized by cells and setae having numerous chloroplasts and being widely distributed.However,the delimitations of some species are problematic because of limited morphological information in the classical descriptions.Monoclonal strains of the section Chaetoceros were established,morphological features were studied using light and electron microscopy,and the hypervariable D 1-D 3 region of the nuclear ribosomal large subunit gene was sequenced to address phylogenetic relationships.Fifteen species belonging to the section Chaetoceros were recorded,including two new species,C.hainanensis sp.nov.and C.tridiscus sp.nov.Chaetoceros hainanensis was characterized by straight chains,narrowly lanceolate to hexagonal apertures,sibling setae diverging in nearly right angles,stipule-shaped spines on terminal setae and arrowhead-shaped spines on intercalary setae.C.tridiscus had short straight chains,narrowly lanceolate apertures,arrowhead-shaped spines and circular poroids arranged in a grid pattern on terminal and intercalary setae.The phylogenetic analyses revealed six groups formed by 19 species within the section Chaetoceros,which was found to be monophyletic.The subdivision of the section is still not well understood.The morphological characters within each group varied considerably and molecular information on more species are needed to enrich the phylogenetic profiling.展开更多
基金Supported by Guizhou Province Science and Technology Innovation Ability Promotion Special Project[2021]1Science and Technology Project of Guizhou Province([2021]YB201,[2019]3007,[2022]YB083)。
文摘[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the collected 52 morel samples were sequenced,and BLAST alignment was performed by NCBI to construct phylogenetic trees.[Results]The collected morel strains were mainly divided into two major groups:namely black morel and yellow morel groups,of which the black morel group was most,mainly including Morchella sextelata,Morchella importuna,Morchella crassipes,and Morchella eximia.There were very few yellow morel strains,including M.crassipes and M.eximia.[Conclusions]This study lays a foundation for the classification and research of morel germplasm resources,and provides a powerful reference value for the research and production of high-quality morel strains.
基金Supported by the Joint Fund of National Natural Science Foundation of China and Chinese Shandong Province(No.U 2106205)the National Natural Science Foundation of China(No.32170206)the National Key Research and Development Program of China(No.2022YFC3105201)。
文摘Chaetoceros Ehrenberg is one of the most diverse genera of planktonic diatoms.The species in section Chaetoceros are characterized by cells and setae having numerous chloroplasts and being widely distributed.However,the delimitations of some species are problematic because of limited morphological information in the classical descriptions.Monoclonal strains of the section Chaetoceros were established,morphological features were studied using light and electron microscopy,and the hypervariable D 1-D 3 region of the nuclear ribosomal large subunit gene was sequenced to address phylogenetic relationships.Fifteen species belonging to the section Chaetoceros were recorded,including two new species,C.hainanensis sp.nov.and C.tridiscus sp.nov.Chaetoceros hainanensis was characterized by straight chains,narrowly lanceolate to hexagonal apertures,sibling setae diverging in nearly right angles,stipule-shaped spines on terminal setae and arrowhead-shaped spines on intercalary setae.C.tridiscus had short straight chains,narrowly lanceolate apertures,arrowhead-shaped spines and circular poroids arranged in a grid pattern on terminal and intercalary setae.The phylogenetic analyses revealed six groups formed by 19 species within the section Chaetoceros,which was found to be monophyletic.The subdivision of the section is still not well understood.The morphological characters within each group varied considerably and molecular information on more species are needed to enrich the phylogenetic profiling.
