Objective To explore the mechanism of electroacupuncture（EA） at ＂Fēnglóng＂（丰隆 ST 40） for hyperlipemia（HLP） in rats.Methods Forty health SD rats were randomly divided into a normal control group（group ...Objective To explore the mechanism of electroacupuncture（EA） at ＂Fēnglóng＂（丰隆 ST 40） for hyperlipemia（HLP） in rats.Methods Forty health SD rats were randomly divided into a normal control group（group A）,a high fat forage group（group B） and a high fat forage ＋ treatment group（group C）,a high fat forage ＋ normal forage group（group D） and a high fat forage ＋ normal forage ＋ treatment group（group E）,eight rats in each group.EA was applied at ＂Fēnglóng＂（丰隆 ST 40） of the rats in group C and group E,once daily.After treatment of 30 days,plasma lipid levels in the rats,including total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C） were detected.Real-time quantitative polymerase chain reaction（RT-PCR） and Western Blotting were applied to detect the gene expression changes of ATP-binding cassette transporter A1（ABCA1）,peroxisome proliferator-activated receptor alpha（PPARα）,liver X receptor alpha（LXR-α） and retinoid X receptor alpha（RXR-α） in liver tissue of rats.Results Compared with group A,contents of TC,LDL-C were significantly elevated in group B and group D（all P〈0.01）;compared with group B,above indices were significantly decreased in group D（all P〈0.01）.After the treatment of EA at ＂Fēnglóng＂（丰隆 ST 40）,contents of TC,LDL-C were significantly decreased（all P〈0.01）,and contents of TG,HDL-C did not change significantly（all P〉0.05）.Compared with group A,mRNA and protein contents of ABCA1,PPARα,LXR-α and RXR-α were decreased significantly in group B and group D（all P〈0.01）.But compared with group B,the above indices were decreased in group D.After the treatment of EA,mRNA and protein contents of ABCA1,PPARα,RXR-α and LXR-α significantly increased（all P〈0.05）.Conclusion EA at ＂Fēnglóng＂（丰隆 ST 40） can decrease contents of blood TC,LDL-C in rats of hyperlipemia and up-regulate the gene expression of ABCA1,PPARα,LXR-α and RXR-α in liver,so as to promote reverse cholesterol transport,with a certain therapeutic effect on hyperlipemia.展开更多
基金Supported by National Natural Science Fund Project of China:30873309
文摘Objective To explore the mechanism of electroacupuncture（EA） at ＂Fēnglóng＂（丰隆 ST 40） for hyperlipemia（HLP） in rats.Methods Forty health SD rats were randomly divided into a normal control group（group A）,a high fat forage group（group B） and a high fat forage ＋ treatment group（group C）,a high fat forage ＋ normal forage group（group D） and a high fat forage ＋ normal forage ＋ treatment group（group E）,eight rats in each group.EA was applied at ＂Fēnglóng＂（丰隆 ST 40） of the rats in group C and group E,once daily.After treatment of 30 days,plasma lipid levels in the rats,including total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C） were detected.Real-time quantitative polymerase chain reaction（RT-PCR） and Western Blotting were applied to detect the gene expression changes of ATP-binding cassette transporter A1（ABCA1）,peroxisome proliferator-activated receptor alpha（PPARα）,liver X receptor alpha（LXR-α） and retinoid X receptor alpha（RXR-α） in liver tissue of rats.Results Compared with group A,contents of TC,LDL-C were significantly elevated in group B and group D（all P〈0.01）;compared with group B,above indices were significantly decreased in group D（all P〈0.01）.After the treatment of EA at ＂Fēnglóng＂（丰隆 ST 40）,contents of TC,LDL-C were significantly decreased（all P〈0.01）,and contents of TG,HDL-C did not change significantly（all P〉0.05）.Compared with group A,mRNA and protein contents of ABCA1,PPARα,LXR-α and RXR-α were decreased significantly in group B and group D（all P〈0.01）.But compared with group B,the above indices were decreased in group D.After the treatment of EA,mRNA and protein contents of ABCA1,PPARα,RXR-α and LXR-α significantly increased（all P〈0.05）.Conclusion EA at ＂Fēnglóng＂（丰隆 ST 40） can decrease contents of blood TC,LDL-C in rats of hyperlipemia and up-regulate the gene expression of ABCA1,PPARα,LXR-α and RXR-α in liver,so as to promote reverse cholesterol transport,with a certain therapeutic effect on hyperlipemia.
