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Analytical characteristics of a qPCR-based molecular diagnostic assay-conceptual considerations for laboratory personnel
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作者 Tanuj Shukla Prabhakar Rao Kaipa +1 位作者 Shesheer K Munpally Rachana Tripathi 《The Journal of Biomedical Research》 CAS 2014年第3期246-248,共3页
Dear Editor:Quantitative real-time PCR has revolutionized molecular diagnostics with its ease of use,increased sensitivity and specificity and low turnaround time.PCR/quantitative PCR(qPCR)-based assays offer a dis... Dear Editor:Quantitative real-time PCR has revolutionized molecular diagnostics with its ease of use,increased sensitivity and specificity and low turnaround time.PCR/quantitative PCR(qPCR)-based assays offer a distinct advantage over other serological/conventional diagnostic approaches.The ability to diagnose infectious diseases has benefited from the availability of US FDA approved and Conformite Europeenne(CE)-marked qPCR-based in-vitro diagnostic kits from international companies.The high-quality kits are calibrated with the World Health Organization(WHO)reference standards and the National Institute for Biological Standards and Control(NIBSC)standards. 展开更多
关键词 qPCR Analytical characteristics of a qPCR-based molecular diagnostic assay-conceptual considerations for laboratory personnel
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