To search for certain signature proteins and the expression profiles in lacrimal passage stenosis,rabbit models of lacrimal passage stenosis were treated by 125I seed brachytherapy.All the signature proteins were sepa...To search for certain signature proteins and the expression profiles in lacrimal passage stenosis,rabbit models of lacrimal passage stenosis were treated by 125I seed brachytherapy.All the signature proteins were separated by two-dimensional electrophoresis,and identified by mass spectrometry.The results show that the up-regulated proteins are peptidyl-prolyl cis-trans isomerase A(PPIase A),and epidermal fatty acid-binding protein(E-FABP),while the down-regulated proteins are myosin light chain 1(isomer of skeletal muscle),myosin light polypeptide 6(isomer 1 of smooth muscle and non-muscle),myosin light chain 1(isomer of slow-twitch muscle A),isomer 2 of ERC protein 2,and α-crystallin family protein.The proteins may play a role in healing the wound and regulating synaptic active zone of neurons due to correlation to cell apoptosis,proliferation and migration of smooth muscle cell.These provide molecular mechanism for preventing stenosis and restenosis of lacrimal passage.展开更多
AIM:To evaluate the effect of reducing the use of antibiotics in the treatment of infant bacterial dacryocystitis after probing of the lacrimal duct.METHODS:A total of 542 cases of children under one year old and acce...AIM:To evaluate the effect of reducing the use of antibiotics in the treatment of infant bacterial dacryocystitis after probing of the lacrimal duct.METHODS:A total of 542 cases of children under one year old and accepting nasolacrimal duct probing treatment were divided into two groups, which were treated with topical and oral antibiotics, respectively.Conjunctival sac secretions were used as a control index of bacterial infection, whereas the disappearance of epiphora symptoms and lacrimal passage patency were used as cure indexes. The χ2test was used to compare enumeration and measurement data, and a statistical significance was set at P 【0.05. The therapeutic effect on the two groups of postoperative patients was investigated.RESULTS:In the two study groups, no significant differences in gender, age and postoperative control of lacrimal sac infection were observed. The cure rates after three probing operations also showed no significant difference.CONCLUSION:After probing of the lacrimal passage,results of this study confirmed that postoperative medication without oral antibiotics but an ophthalmic dosage of antibiotics was a simple and effective treatment method.展开更多
The diagnosis and treatment of the lacrimal passage obstruction with lacrimal endoscope was investigated and its subsidiary surgical procedures were evaluated. Ninety-three patients （109 eyes） with lacrimal passage ...The diagnosis and treatment of the lacrimal passage obstruction with lacrimal endoscope was investigated and its subsidiary surgical procedures were evaluated. Ninety-three patients （109 eyes） with lacrimal passage obstruction, including presaccal canalicular obstruction （PSCO） and nasolacrimal duct obstruction （NLDO）, were examined under a lacrimal endoscope, and the obstruc- tions were treated with laser or micro-drill. All patients were followed up after the operation for 3--6 months. The difference between the laser and the micro-drill treatment was observed. During the period of follow-up, the curative rate was 82.57%. The healing rate in PSCO group and NLDO was 80.36% and 84.91% respectively （P〉0.05）. Alter treatment with the laser, the healing rate was 93.33% in the PSCO group and 66.67% in the NLDO group respectively （P〈0.05）. After treatment with the micro-drill, the healing rate in PSCO and NLDO groups was 65.39% and 94.28% respec- tively （P〈0.01）. The lacrimal passage obstruction can be observed and treated directly through the lacrimal endoscope. Choosing different surgical procedures in operation according to the locations of the obstruction is helpful to improve the effectiveness.展开更多
To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(Ne...To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(New Zealand),including the nasolacrimal duct stenosis,the mechanical probing with outer layer of thermoplastic tube,and the ^(125)I radioactive probing with the ^(125)I seeds sealing into the thermoplastic tube.After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained,and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis.Comparing with the blank control,the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated(p<0.05),but in the ^(125)I radioactive probing are down-/up-regulated(p<0.05) and can be used to re-canalize the stenostic lacrimal passage.The results show that the ^(125)I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis.展开更多
基金Supported by Nature Science Foundation of Jilin Province(No.20075323)Project of Science and Technology of Changchun City(2007GH25)
文摘To search for certain signature proteins and the expression profiles in lacrimal passage stenosis,rabbit models of lacrimal passage stenosis were treated by 125I seed brachytherapy.All the signature proteins were separated by two-dimensional electrophoresis,and identified by mass spectrometry.The results show that the up-regulated proteins are peptidyl-prolyl cis-trans isomerase A(PPIase A),and epidermal fatty acid-binding protein(E-FABP),while the down-regulated proteins are myosin light chain 1(isomer of skeletal muscle),myosin light polypeptide 6(isomer 1 of smooth muscle and non-muscle),myosin light chain 1(isomer of slow-twitch muscle A),isomer 2 of ERC protein 2,and α-crystallin family protein.The proteins may play a role in healing the wound and regulating synaptic active zone of neurons due to correlation to cell apoptosis,proliferation and migration of smooth muscle cell.These provide molecular mechanism for preventing stenosis and restenosis of lacrimal passage.
文摘AIM:To evaluate the effect of reducing the use of antibiotics in the treatment of infant bacterial dacryocystitis after probing of the lacrimal duct.METHODS:A total of 542 cases of children under one year old and accepting nasolacrimal duct probing treatment were divided into two groups, which were treated with topical and oral antibiotics, respectively.Conjunctival sac secretions were used as a control index of bacterial infection, whereas the disappearance of epiphora symptoms and lacrimal passage patency were used as cure indexes. The χ2test was used to compare enumeration and measurement data, and a statistical significance was set at P 【0.05. The therapeutic effect on the two groups of postoperative patients was investigated.RESULTS:In the two study groups, no significant differences in gender, age and postoperative control of lacrimal sac infection were observed. The cure rates after three probing operations also showed no significant difference.CONCLUSION:After probing of the lacrimal passage,results of this study confirmed that postoperative medication without oral antibiotics but an ophthalmic dosage of antibiotics was a simple and effective treatment method.
文摘The diagnosis and treatment of the lacrimal passage obstruction with lacrimal endoscope was investigated and its subsidiary surgical procedures were evaluated. Ninety-three patients （109 eyes） with lacrimal passage obstruction, including presaccal canalicular obstruction （PSCO） and nasolacrimal duct obstruction （NLDO）, were examined under a lacrimal endoscope, and the obstruc- tions were treated with laser or micro-drill. All patients were followed up after the operation for 3--6 months. The difference between the laser and the micro-drill treatment was observed. During the period of follow-up, the curative rate was 82.57%. The healing rate in PSCO group and NLDO was 80.36% and 84.91% respectively （P〉0.05）. Alter treatment with the laser, the healing rate was 93.33% in the PSCO group and 66.67% in the NLDO group respectively （P〈0.05）. After treatment with the micro-drill, the healing rate in PSCO and NLDO groups was 65.39% and 94.28% respec- tively （P〈0.01）. The lacrimal passage obstruction can be observed and treated directly through the lacrimal endoscope. Choosing different surgical procedures in operation according to the locations of the obstruction is helpful to improve the effectiveness.
基金Supported by Natural Science Foundation of Jilin Province(No.200705327)Project of Science and Technology of Changchun City(2007GH25)
文摘To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(New Zealand),including the nasolacrimal duct stenosis,the mechanical probing with outer layer of thermoplastic tube,and the ^(125)I radioactive probing with the ^(125)I seeds sealing into the thermoplastic tube.After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained,and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis.Comparing with the blank control,the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated(p<0.05),but in the ^(125)I radioactive probing are down-/up-regulated(p<0.05) and can be used to re-canalize the stenostic lacrimal passage.The results show that the ^(125)I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis.
