目的:探讨早产儿振幅整合脑电图(aEEG)评分、血清乳酸脱氢酶(LDH)水平与脑损伤严重程度相关性,并分析其对脑损伤发生的评估价值。方法:回顾性选取2021年1月至2023年6月于漯河市中心医院出生的早产儿212例为研究对象,依据出生后是否发生...目的:探讨早产儿振幅整合脑电图(aEEG)评分、血清乳酸脱氢酶(LDH)水平与脑损伤严重程度相关性,并分析其对脑损伤发生的评估价值。方法:回顾性选取2021年1月至2023年6月于漯河市中心医院出生的早产儿212例为研究对象,依据出生后是否发生脑损伤分为发生组86例、未发生组126例。比较两组aEEG评分、血清LDH水平及出生后5 min Apgar评分、新生儿神经学评分(NBNA)。依据脑损伤严重程度脑损伤发生组分为轻度27例、中度38例、重度21例,比较两组aEEG评分、血清LDH水平,从而分析aEEG评分、血清LDH水平与脑损伤严重程度、出生后5 min Apgar评分、NBNA评分相关性,分析脑损伤发生的影响因素,分析aEEG评分、血清LDH水平对脑损伤的评估价值。结果:发生组aEEG评分、出生后5 min Apgar评分、NBNA评分低于未发生组,血清LDH水平高于未发生组(P<0.05);aEEG评分与出生后5 min Apgar评分、NBNA评分呈正相关,而与脑损伤严重程度呈负相关,血清LDH水平与之相反(P<0.05);妊娠期高血压疾病、妊娠期糖尿病、血清LDH水平为脑损伤发生的独立危险因素,产前使用糖皮质激素、aEEG评分为脑损伤发生的独立保护因素(P<0.05);出生后12 h aEEG评分、血清LDH水平联合检测评估脑损伤发生的AUC大于单项指标评估(P<0.05)。结论:aEEG评分、血清LDH水平与早产儿脑损伤严重程度密切相关,联合检测对脑损伤发生具有一定评估价值。展开更多
Objective: Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B(LDHB) has been detected in breast cancer but the function of LDHB remains unknown.Methods: Weste...Objective: Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B(LDHB) has been detected in breast cancer but the function of LDHB remains unknown.Methods: Western blot was used to analyze LDHB expression in breast cancer cells. The impact of LDHB on tumor cell migration and invasion was determined using Transwell assays, wound healing assays, and a mouse lung metastasis model. Subcutaneous tumor formation, a natural killer(NK) cell cytotoxicity assay, and flow cytometry evaluated NK cell activation. Immunofluorescence and quantitative real-time PCR detected NK cell activation markers. Kaplan-Meier analysis evaluated the effect of immune cell infiltration on prognosis. Single-sample gene set enrichment analysis determined NK cell activation scores. A support vector machine predicted the role of LDHB in NK cell activation.Results: In this study we showed that LDHB inhibits the breast cancer cell metastasis and orchestrates metabolic reprogramming within tumor cells. Our results revealed that LDHB-mediated lactic acid clearance in breast cancer cells triggers NK cell activation within the tumor microenvironment. Our findings, which were confirmed in a murine model, demonstrated that LDHB in tumor cells promotes NK cell activation and ultimately results in the eradication of malignant cells. Clinically, our study further validated that LDHB affects immune cell infiltration and function. Specifically, its expression has been linked to enhanced NK cell-mediated cytotoxicity and improved patient survival. Furthermore, we identified LDHB expression in tumors as an important predictor of NK cell activation, with strong predictive ability in some cancers.Conclusions: Our results suggest that LDHB is a promising target for activating the tumor immune microenvironment in breast cancer, where LDHB-associated lactic acid clearance leads to increased NK cell activity. This study highlights the critical role of LDHB in regulating immune responses and its potential as a therapeutic target for breast cancer.展开更多
文摘目的:探讨早产儿振幅整合脑电图(aEEG)评分、血清乳酸脱氢酶(LDH)水平与脑损伤严重程度相关性,并分析其对脑损伤发生的评估价值。方法:回顾性选取2021年1月至2023年6月于漯河市中心医院出生的早产儿212例为研究对象,依据出生后是否发生脑损伤分为发生组86例、未发生组126例。比较两组aEEG评分、血清LDH水平及出生后5 min Apgar评分、新生儿神经学评分(NBNA)。依据脑损伤严重程度脑损伤发生组分为轻度27例、中度38例、重度21例,比较两组aEEG评分、血清LDH水平,从而分析aEEG评分、血清LDH水平与脑损伤严重程度、出生后5 min Apgar评分、NBNA评分相关性,分析脑损伤发生的影响因素,分析aEEG评分、血清LDH水平对脑损伤的评估价值。结果:发生组aEEG评分、出生后5 min Apgar评分、NBNA评分低于未发生组,血清LDH水平高于未发生组(P<0.05);aEEG评分与出生后5 min Apgar评分、NBNA评分呈正相关,而与脑损伤严重程度呈负相关,血清LDH水平与之相反(P<0.05);妊娠期高血压疾病、妊娠期糖尿病、血清LDH水平为脑损伤发生的独立危险因素,产前使用糖皮质激素、aEEG评分为脑损伤发生的独立保护因素(P<0.05);出生后12 h aEEG评分、血清LDH水平联合检测评估脑损伤发生的AUC大于单项指标评估(P<0.05)。结论:aEEG评分、血清LDH水平与早产儿脑损伤严重程度密切相关,联合检测对脑损伤发生具有一定评估价值。
基金supported by the Shenzhen Science and Technology Program (Grant no. JCYJ20230807090459001)the Joint Research Fund of the National Science Fund of China Science and Technology Development Fund of Macao SAR (No. 32161160303 for NSFC and No. 0010/2021/AFJ for FDCT)the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University (Grant no. ZNJC202330)。
文摘Objective: Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B(LDHB) has been detected in breast cancer but the function of LDHB remains unknown.Methods: Western blot was used to analyze LDHB expression in breast cancer cells. The impact of LDHB on tumor cell migration and invasion was determined using Transwell assays, wound healing assays, and a mouse lung metastasis model. Subcutaneous tumor formation, a natural killer(NK) cell cytotoxicity assay, and flow cytometry evaluated NK cell activation. Immunofluorescence and quantitative real-time PCR detected NK cell activation markers. Kaplan-Meier analysis evaluated the effect of immune cell infiltration on prognosis. Single-sample gene set enrichment analysis determined NK cell activation scores. A support vector machine predicted the role of LDHB in NK cell activation.Results: In this study we showed that LDHB inhibits the breast cancer cell metastasis and orchestrates metabolic reprogramming within tumor cells. Our results revealed that LDHB-mediated lactic acid clearance in breast cancer cells triggers NK cell activation within the tumor microenvironment. Our findings, which were confirmed in a murine model, demonstrated that LDHB in tumor cells promotes NK cell activation and ultimately results in the eradication of malignant cells. Clinically, our study further validated that LDHB affects immune cell infiltration and function. Specifically, its expression has been linked to enhanced NK cell-mediated cytotoxicity and improved patient survival. Furthermore, we identified LDHB expression in tumors as an important predictor of NK cell activation, with strong predictive ability in some cancers.Conclusions: Our results suggest that LDHB is a promising target for activating the tumor immune microenvironment in breast cancer, where LDHB-associated lactic acid clearance leads to increased NK cell activity. This study highlights the critical role of LDHB in regulating immune responses and its potential as a therapeutic target for breast cancer.
