Low-ion-density discontinuous polyacrylamide electrophoresis (pH 8.0) of LDH from different tissuse of four species (Tetrastes bonasia,Chrysolophus pictus, Phasians colchicus, Gallus gallus domesticus) in Galliformes...Low-ion-density discontinuous polyacrylamide electrophoresis (pH 8.0) of LDH from different tissuse of four species (Tetrastes bonasia,Chrysolophus pictus, Phasians colchicus, Gallus gallus domesticus) in Galliformes showed that this electrophoresis system was suitable for analysing avian LDH. Thermostabilty to heat and nonsusceptibility to urea inhibition of low-density urea of the four liver LDHs were in such order as LDH1> LDH2> LDH3> LDH4> LDH5. And it appeared. by comparing relative movable ratio of A and B subunits of LDHs, that difference exists between Phasianidae and Tetraonidae and in Phasianidae Phasianus was closer to Chrysolophus than to Gallus.展开更多
The wild type strain Rhizopus oryzae PW352 was mutated by means of nitrogen ion implantation （15 keV, 7.8×10^14 ～ 2.08 ×10^15 ions/cm^2） to find an industrial strain with a higher L（＋）-lactic acid yiel...The wild type strain Rhizopus oryzae PW352 was mutated by means of nitrogen ion implantation （15 keV, 7.8×10^14 ～ 2.08 ×10^15 ions/cm^2） to find an industrial strain with a higher L（＋）-lactic acid yield, and two mutants RE3303 and RF9052 were isolated. In order to discuss the mechanism primarily, Lactate Dehydrogenase of Rhizopus oryzae was studied. While the two mutants produced L（＋）-lactic acid by 75% more than the wild strain did, their specific activity of Lactate Dehydrogenase was found to be higher than that in the wild strain. The optimum temperature of Lactate Dehydrogenase in Rhizopus oryzae RF9052 was higher. Compared to the wild strain, the Michaelis constant （Km） value of Lactate Dehydrogenase in the mutants was Changed. All these changes show that L（＋）-lactic acid production has a correlation with the specific activity of Lactate Dehydrogenase. The low-energy ions, implanted into the strain, may improve the specific activity of Lactate Dehydrogenase by influencing its gene structure and protein structure.展开更多
文摘Low-ion-density discontinuous polyacrylamide electrophoresis (pH 8.0) of LDH from different tissuse of four species (Tetrastes bonasia,Chrysolophus pictus, Phasians colchicus, Gallus gallus domesticus) in Galliformes showed that this electrophoresis system was suitable for analysing avian LDH. Thermostabilty to heat and nonsusceptibility to urea inhibition of low-density urea of the four liver LDHs were in such order as LDH1> LDH2> LDH3> LDH4> LDH5. And it appeared. by comparing relative movable ratio of A and B subunits of LDHs, that difference exists between Phasianidae and Tetraonidae and in Phasianidae Phasianus was closer to Chrysolophus than to Gallus.
基金National Natural Science Foundation of China(No.20576132)
文摘The wild type strain Rhizopus oryzae PW352 was mutated by means of nitrogen ion implantation （15 keV, 7.8×10^14 ～ 2.08 ×10^15 ions/cm^2） to find an industrial strain with a higher L（＋）-lactic acid yield, and two mutants RE3303 and RF9052 were isolated. In order to discuss the mechanism primarily, Lactate Dehydrogenase of Rhizopus oryzae was studied. While the two mutants produced L（＋）-lactic acid by 75% more than the wild strain did, their specific activity of Lactate Dehydrogenase was found to be higher than that in the wild strain. The optimum temperature of Lactate Dehydrogenase in Rhizopus oryzae RF9052 was higher. Compared to the wild strain, the Michaelis constant （Km） value of Lactate Dehydrogenase in the mutants was Changed. All these changes show that L（＋）-lactic acid production has a correlation with the specific activity of Lactate Dehydrogenase. The low-energy ions, implanted into the strain, may improve the specific activity of Lactate Dehydrogenase by influencing its gene structure and protein structure.
