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Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri:intraspecific and interspecific patterns
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作者 Chunxiu Lin Yuxing Zheng +5 位作者 Bo Zhang Guopeng Lin Kexin Shang Jianxin Zhao Gang Wang Wei Chen 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期2799-2812,共14页
Lactobacillus rhamnosus(Rh)and Lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)research.The variations between these species’efficacy against colitis,and their model of act... Lactobacillus rhamnosus(Rh)and Lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)research.The variations between these species’efficacy against colitis,and their model of action in this regard,are intriguing and enable treatment to be individually tailored to patients.In this study,four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced.The anti-colitis activities of both strains involved various aspects of intestinal immune,physical,chemical,and biological barrier function.Strikingly,the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration.Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis.Nevertheless,Re strains were more effective than Rh strains in inhibiting production of inflammatory factors;promoting production of intestinal mucus,antimicrobial peptides,and tight junction proteins;and supporting the stem cell compartment.This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains.In addition,the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism-and prophage-related genes,respectively.Taken together,the results of this study could assist researchers in developing effective therapies for IBD. 展开更多
关键词 Inflammatory bowel disease Anti-colitis lactobacillus rhamnosus lactobacillus reuteri Intestinal barrier function Short-chain fatty acids
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Identification,Characterization,and Probiotic Potentials of Lactobacillus pentosus SF-1
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作者 JIAO Lin LIANG Shufei +4 位作者 CHU Ruixia WANG Wentao MAI Kangsen LIU Ming WAN Min 《Journal of Ocean University of China》 CAS CSCD 2024年第2期509-517,共9页
In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,name... In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and biochemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4 h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6 h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high potential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zebrafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incubation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protection of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture. 展开更多
关键词 lactobacillus pentosus PROBIOTICS ANTIBIOTICS HEMOLYSIS cell surface characteristics bactericidal activity INFECTION
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Effect of dietary supplement of inactivated Lactobacillus plantarum Ep-M 17 on growth performance,immune response,disease resistance,and intestinal microbiota in Penaeus vannamei
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作者 Ting WU Xiaoman HU +2 位作者 Wenlong XU Yang DU Jiong CHEN 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第2期676-694,共19页
Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of Penaeus vannamei.However,high temperature and pressure du... Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of Penaeus vannamei.However,high temperature and pressure during feed pelletizing is the main problem that can lead to a decrease in the activity of probiotics or cause their inactivation.Further investigation needs to investigate whether inactivated Ep-M17 can exert similar effects as live Ep-M17.Therefore,we evaluated the effects of inactivated L.plantarum Ep-M17 on growth performance,immune response,disease resistance,and gut microbiota in P.vannamei.Results show that adding inactivated Ep-M17 to the feed also promoted body weight gain and increased relative immune protection in shrimp.Also,histological examination revealed that the administration of inactivated Ep-M17 led to improvements in the density and distribution of microvilli in the intestines and enhancements in the abundance of B and R cells in the hepatopancreas.Additionally,the inactivated Ep-M17 supplementation resulted in increased activity levels of nutrient immune-related enzymes in both the shrimp hepatopancreas and intestines.Moreover,it stimulated the expression of Lvlec,PEN-3a,Crustin,LGBP,Lysozyme,and proPo genes in both the hepatopancreas and intestines.Furthermore,the inactivated Ep-M17 also increased bacterial diversity in the gut of shrimp and promoted the abundance of specific flora,facilitating the host organism’s metabolism and immunity to improve the disease resistance of shrimp.Therefore,supplementation of inactivated L.plantarum Ep-M17 in shrimp diets can exert similar effects as live L.plantarum Ep-M17 effectively improving growth performance,gut microbiota,immune response,and disease resistance in P.vannamei. 展开更多
关键词 Penaeus vannamei lactobacillus plantarum Ep-M17 inactivated probiotics immune response gut flora
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Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp.bulgaricus in skimmed milk
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作者 Yue Yang Wenxia Zhou +3 位作者 Lingyu Yang Yilun Chen Dongxiao Sun-Waterhouse Dapeng Li 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期3018-3030,共13页
Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products... Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products remain unclear.This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide,chlorpyrifos,in milk by Lactobacillus delbrueckii ssp.bulgaricus using gas chromatography-tandem mass spectrometry(GC-MS/MS)combined with transcriptome analysis.After the strain was cultured for 20 h in the presence of chlorpyrifos,differential expressions of 383 genes were detected,including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase,phosphoesterase,diphosphatase,oxidoreductase,dehydratase,as well as membrane transporters.GC-MS/MS analysis revealed the changes of secondary metabolites in L.bulgaricus during milk fermentation due to chlorpyrifos stress.6-Methylhexahydro-2H-azepin-2-one,2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates,along with the proposed pathways,might be involved in chlorpyrifos biodegradation by L.bulgaricus. 展开更多
关键词 lactobacillus delbrueckii ssp.bulgaricus Biodegradation CHLORPYRIFOS TRANSCRIPTOMICS METABOLOME
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Lactobacillus plantarum AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway
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作者 Hongyun Zhang Guangqiang Wang +6 位作者 Zhiqiang Xiong Zhuan Liao Yangyan Qian Xin Song Li Sui Lianzhong Ai Yongjun Xia 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期698-708,共11页
Probiotics have great potential in regulating intestinal pain.In this study,the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome(IBS)rats were studied... Probiotics have great potential in regulating intestinal pain.In this study,the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome(IBS)rats were studied.The results showed that tryptase released after mast cell activation and degranulation plays a key role in visceral pain,and L.plantarum AR495 reduced the stimulation of colonic mast cells and the expression of protease-activated receptor 2(PAR2)and TRPV1 in dorsal root ganglia.Research further showed that supplementation with L.plantarum AR495 increased the level of short-chain fatty acids(SCFAs)and enhanced the barrier function of the colon.In addition,the microbiota analysis of the colon indicated that L.plantarum AR495 promoted the proliferation of Bifidobacterium and inhibited the proliferation of Lachnospiraceae,which alleviated the imbalance of the intestinal microbiota caused by IBS to a certain extent.In total,L.plantarum AR495 might reduce visceral sensitivity through the Mast cell-PAR2-TRPV1 signaling pathway by maintaining the homeostasis of the intestinal barrier. 展开更多
关键词 lactobacillus plantarum Irritable bowel syndrome Visceral sensitivity Dorsal root ganglia TRYPTASE MICROBIOTA
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Lactobacillus from fermented bamboo shoots prevents inflammation in DSS-induced colitis mice via modulating gut microbiome and serum metabolites
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作者 Xiangru Liu Xiaoling Lu +6 位作者 Hao Nie Jing Yan Zhiwen Ma Hailin Li Shixin Tang Qi Yin Jingfu Qiu 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期2833-2846,共14页
Fermented bamboo shoots(FBS)is a region-specific food widely consumed in Southwestern China,with Lactobacillus as the predominant fermenting bacteria.However,the probiotic potential of Lactobacillus derived from FBS r... Fermented bamboo shoots(FBS)is a region-specific food widely consumed in Southwestern China,with Lactobacillus as the predominant fermenting bacteria.However,the probiotic potential of Lactobacillus derived from FBS reminds largely unexplored,especially for diseases with a low prevalence in areas consuming FBS,namely,inflammatory bowel disease.In this study,Lactiplantibacillus pentosus YQ001 and Lentilactobacillus senioris YQ005 were screening by in vitro probiotic tests to further investigate the probioticlike bioactivity in dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)mouse.They exhibited more positive probiotic effects than Lactobacillus rhamnosus GG in preventing intestinal inflammatory response.The results revealed that both strains improved the abundance of deficient intestinal microbiota in UC mice,including Muribaculaceae and Akkermansia.In the serum metabolome,they modulated the DSS-disturbed levels of metabolites,with significant increment of cinnamic acid.Meanwhile,they reduced the expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6)inflammatory factors and increased zonula occludens-1(ZO-1),Occludin,and cathelicidin-related antimicrobial peptide(CRAMP)in the colon.Consequently,these results demonstrated that Lactobacillus spp.isolates derived from FBS showed promising probiotic activity based on the gut microbiome homeostasis modulation,anti-inflammation and intestinal barrier protection in UC mice. 展开更多
关键词 Fermented bamboo shoots lactobacillus Probiotic activity Gut microbiome modulation Ulcerative colitis
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Demonstration of safety characteristics and effects on gut microbiota of Lactobacillus gasseri HMV18
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作者 Xianxian Jia Miaomiao Jia +10 位作者 Xiang Gao Xiang Li Mengyuan Wang Shengqiang Du Rui Huang Xiaotong Li Jun Zhang Shujin Li Chunling Ma Yan Zhang Bin Cong 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期611-620,共10页
Human normal flora is a source of probiotics.The safety characteristics of a specific isolate determine its application in foods or drugs.The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of... Human normal flora is a source of probiotics.The safety characteristics of a specific isolate determine its application in foods or drugs.The food-borne-pathogen antagonist strain Lactobacillus gasseri HMV18 is one of the isolates from normal human flora.In this work,we assessed the in vitro pH tolerance,bile tolerance,biogenic amine production,mucin utilization,and safety of in vivo administration to mice to evaluate general health,organ-body weight index,organ histopathological change,whether L.gasseri HMV18 can colonize in the gut or modulate the gut microbiota after oral administration.The results suggest that L.gasseri HMV18 can tolerate pH 3 for 2 h,3%bile for 3 h,biogenic amine negative,mucin usage negative,does not encode verified toxins,and cause no visible change in mice's organs.L.gasseri HMV18 might not colonize in mice's gut,but can significantly affect the structure of gut microbiota.A bibliographical survey suggested that there were as few as 8 opportunistic infection cases from 1984 to 2022 and that the possibility for L.gasseri to cause infection is relatively low.Therefore,this work provides a basis for the foods or drugs application of L.gasseri HMV18 and gives a map of experiments for the safety assessment of probiotics. 展开更多
关键词 PROBIOTICS lactobacillus gasseri Safety assessment Gut microbiota
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Effects of Lactobacillus paracasei N1115 on gut microbial imbalance and liver function in patients with hepatitis B-related cirrhosis
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作者 Yan-Chao Hu Xiang-Chun Ding +3 位作者 Hui-Juan Liu Wan-Long Ma Xue-Yan Feng Li-Na Ma 《World Journal of Gastroenterology》 SCIE CAS 2024年第11期1556-1571,共16页
BACKGROUND Hepatitis B cirrhosis(HBC)is a chronic disease characterized by irreversible diffuse liver damage and aggravated by intestinal microbial imbalance and metabolic dysfunction.Although the relationship between... BACKGROUND Hepatitis B cirrhosis(HBC)is a chronic disease characterized by irreversible diffuse liver damage and aggravated by intestinal microbial imbalance and metabolic dysfunction.Although the relationship between certain single probiotics and HBC has been explored,the impact of the complex ready-to-eat Lactobacillus paracasei N1115(LP N1115)supplement on patients with HBC has not been determined.AIM To compare the changes in the microbiota,inflammatory factor levels,and liver function before and after probiotic treatment in HBC patients.METHODS This study included 160 HBC patients diagnosed at the General Hospital of Ningxia Medical University between October 2018 and December 2020.Patients were randomly divided into an intervention group that received LP N1115 supplementation and routine treatment and a control group that received routine treatment only.Fecal samples were collected at the onset and conclusion of the 12-wk intervention period.The structure of the intestinal microbiota and the levels of serological indicators,such as liver function and inflammatory factors,were assessed.RESULTS Following LP N1115 intervention,the intestinal microbial diversity significantly increased in the intervention group(P<0.05),and the structure of the intestinal microbiota was characterized by an increase in the proportions of probiotic microbes and a reduction in harmful bacteria.Additionally,the intervention group demonstrated notable improvements in liver function indices and significantly lower levels of inflammatory factors(P<0.05).CONCLUSION LP N1115 is a promising treatment for ameliorating intestinal microbial imbalance in HBC patients by modulating the structure of the intestinal microbiota,improving liver function,and reducing inflammatory factor levels. 展开更多
关键词 Hepatitis B cirrhosis N1115 ready-to-eat lactobacillus Inflammation Liver function Lachnospiraceae incertae sedis Probiotic
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Antiseptic Efficacy of A Soap Made from Biosurfactants Isolated from Bacillus and Lactobacillus against Pathogenic Bacteria
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作者 Frédéric Yannick Okouakoua Christian Aimé Kayath +10 位作者 Nicaise Saturnin Mokémiabeka Varelle Bervanie Ngala Elenga Digne Nedjea N’goma-Mona Ndelani Nkalla Lambi Sandra Paola Elenga Wilson Christ Dieuveil Bayakissa Malanda Rodinet Tsana Junior Patrick Sergy Bissoko1 Moïse Doria Kaya-Ongoto Duchel Jeanedvi Kinavouidi Etienne Nguimbi 《Advances in Microbiology》 CAS 2024年第1期31-58,共28页
The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological technique... The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological techniques (30 Bacillus and 30 Lactobacillus) and the ability to produce biosurfactants was demonstrated by a hydrocarbon emulsification index (E24). The emulsification indexes (E24) varied from 9% to 100% for Bacillus and from 33% to 100% for Lactobacillus as well. The antagonistic assay showed that biosurfactants were able to inhibit the formation of biofilms and growth of pathogens such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella typhirium, Shigella boydii and Proteus mirabilis. The biosurfactant consortium (BioC) from Bacillus consortium and from Lactobacillus was able to inhibit biofilm formation and the pathogens growth. The BioC was stable to alkaline pH and the temperatures stability of Biosurfactant was ranging from 50°C to 90°C. The soap was made by the cold saponification process using one biosurfactant consortium formulated. This soap has a pH of 10 and showed good cleaning power and good foam stability. Similarly, the soap showed good antiseptic power and disinfection power against all pathogens tested. Handwashing is critical to preventing disease transmission. The persistence of pathogens in waste water was evaluated. The BioS produced showed good disinfection power against all pathogens tested. The valor of reduction on the hands and in the waste water was significantly more than compared to the control soaps used. This soap could be used in the prevention, fighting, and treatment of bacterial and viral infections. 展开更多
关键词 ANTISEPTIC SOAP Biosurfactants BACILLUS lactobacillus DISINFECTION Pathogens bacteria
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Impact of Dietary Lactobacillus plantarum Postbiotics on the Performance of Layer Hens under Heat Stress Conditions
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作者 Mohamad Farran Bouchra El Masry +1 位作者 Zeinab Kaouk Houssam Shaib 《Open Journal of Veterinary Medicine》 CAS 2024年第3期39-55,共17页
This experiment was conducted to determine the performance of heat-stressed layers fed a diet containing the probiotic Lactobacillus plantarum RS5 or its products of fermentation (postbiotics). Twenty-week-old Isa Whi... This experiment was conducted to determine the performance of heat-stressed layers fed a diet containing the probiotic Lactobacillus plantarum RS5 or its products of fermentation (postbiotics). Twenty-week-old Isa White layers, were subdivided into six treatments of 32 individually caged birds. Half of the birds were reared under regular temperature conditions, while the other half was subjected to cyclic daily heat stress. Layers were offered one of three diets: 1) Control;2) Control + Lactobacillus plantarum RS5 probiotic;3) Control + Lactobacillus plantarum RS5 postbiotics. Birds were tested for performance and visceral organ development for 5 months. Heat stress negatively affected the birds’ feed intake, egg weight, shell weight percentage, Haugh unit, shell thickness, yolk color, body weight and spleen weight percentage. Postbiotics significantly increased egg production (p < 0.05) in comparison to the control and the probiotic fed group (94.8% vs 92.6% vs 93.1%, respectively). Birds under probiotic or postbiotic diet showed a significantly higher (p < 0.05) feed intake and egg weight, although the probiotic had a more pronounced and gradual effect. Specific gravity, yolk weight percentage and shell thickness didn’t show differences among dietary groups. The Haugh Unit was significantly higher (p < 0.05) in probiotic group which also showed a significantly lower yolk color index (p < 0.05). The different feed treatments did not impact the bird’s viscera weight percentage, except for the ileum that was significantly lower (p < 0.05) under postbiotic supplementation. Both probiotics and postbiotics could be used as a potential growth promoters and might alleviate heat stress impact in poultry industry. 展开更多
关键词 lactobacillus plantarum LAYERS Heat Stress Postbiotic PROBIOTICS PERFORMANCE
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Lactobacillus delbrueckii subsp.bulgaricus KSFY07对D-半乳糖诱导的氧化衰老小鼠运动能力的提升作用 被引量:1
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作者 邓潇潇 周雅琳 +1 位作者 冯霞 赵欣 《食品与发酵工业》 CAS CSCD 北大核心 2023年第7期49-58,共10页
该研究通过D-半乳糖建立小鼠衰老模型,观察了一株新发现的乳酸菌(Lactobacillus delbrueckii subsp.bulgaricus KSFY07,LDSBKSFY07)对衰老小鼠运动能力提升的作用并阐述了其作用机制。研究通过观察衰老小鼠跑步能力、游泳耐力、血液生... 该研究通过D-半乳糖建立小鼠衰老模型,观察了一株新发现的乳酸菌(Lactobacillus delbrueckii subsp.bulgaricus KSFY07,LDSBKSFY07)对衰老小鼠运动能力提升的作用并阐述了其作用机制。研究通过观察衰老小鼠跑步能力、游泳耐力、血液生化指标、组织病理学变化和mRNA表达变化、小鼠肠道内容物微生物变化来检测LDSBKSFY07提升小鼠运动能力的作用及机制。动物实验结果显示LDSBKSFY07(1.5×10^(9) CFU/kg BW灌胃)能够延长衰老小鼠的跑步时间和力竭游泳时间,降低衰老小鼠血清中的血尿素氮、血乳酸、丙二醛的水平,增加肝糖原、肌糖原的水平及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶的酶活力。组织病理学观察提示LDSBKSFY07减轻了小鼠肝脏和肾脏肾组织的衰老损伤。qPCR结果显示LDSBKSFY07具备下调衰老小鼠肝脏、肾脏和骨骼肌组织诱导型一氧化氮合酶、肿瘤坏死因子α的mRNA表达和上调神经型一氧化氮合酶、Cu/Zn-SOD、Mn-SOD、过氧化氢酶表达的能力。另外,LDSBKSFY07还可以下调衰老小鼠骨骼肌组织的syncytin-1 mRNA表达。同时,LDSBKSFY07还增加了衰老小鼠肠道中厚壁菌门、乳酸杆菌、双歧杆菌的丰度和降低了拟杆菌门的丰度。而且,LDSBKSFY07对组织、血清和肠道菌群状态的调节作用优于维生素C(150 mg/kg BW灌胃)具有减缓小鼠衰老和提升其运动能力的效果。 展开更多
关键词 lactobacillus delbrueckii subsp.bulgaricus 衰老 运动 维生素C MRNA表达
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干酪乳杆菌(Lactobacillus casei)FV006对发酵食品中生物胺的降解性能 被引量:1
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作者 王雪郦 雷超 +4 位作者 申开卫 程艳薇 刘雪婷 李珍 俞露 《食品工业科技》 CAS 北大核心 2023年第14期137-144,共8页
为验证干酪乳杆菌(Lactobacillus casei)对生物胺的降解性能及潜在应用价值。本研究利用高效液相色谱法,探究该菌在液态培养条件下的降胺性能和应用于贵州6种市售发酵食品中的降胺效果。结果表明,在液态培养试验中该菌对组胺、亚精胺、... 为验证干酪乳杆菌(Lactobacillus casei)对生物胺的降解性能及潜在应用价值。本研究利用高效液相色谱法,探究该菌在液态培养条件下的降胺性能和应用于贵州6种市售发酵食品中的降胺效果。结果表明,在液态培养试验中该菌对组胺、亚精胺、苯乙胺、腐胺、尸胺、酪胺和色胺的降胺率分别为9.44%、23.39%、16.27%、13.68%、19.14%、18.16%和12.45%。此外,研究发现该菌对道菜中色胺的降胺率为56.68%;对豆瓣酱中尸胺、组胺和亚精胺的降胺率分别为68.06%、74.52%和85.82%;对酱油中色胺和尸胺的降胺率为66.75%%、81.53%;对泡菜中色胺的降胺率为45.74%;对鱼罐头中色胺、尸胺、组胺和亚精胺的降胺率分别为55.66%、63.92%、45.96%、52.62%;对香肠中色胺、组胺和亚精胺的降胺率分别为71.15%、56.26%、75.54%。研究还发现该菌既具有产生物胺的能力,也具有降解生物胺的能力,且对不同食品中同一种生物胺的降解具有差异性。本研究为干酪乳杆菌(Lactobacillus casei)在低胺发酵食品中的应用提供理论依据。 展开更多
关键词 干酪乳杆菌(lactobacillus casei) 发酵食品 生物胺 降胺率
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Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466^(T) in the gut of mice 被引量:8
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作者 Zhihua Niu Meijuan Zou +5 位作者 Tingting Bei Na Zhang Dongyao Li Miaoshu Wang Chen Li Hongtao Tian 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期607-613,共7页
Lactobacillus rhamnosus and fructooligosaccharides(FOS)have been widely studied so far.However,the effects of L.rhamnosus on the intestinal microecological environment at the species level and the effect of different ... Lactobacillus rhamnosus and fructooligosaccharides(FOS)have been widely studied so far.However,the effects of L.rhamnosus on the intestinal microecological environment at the species level and the effect of different proportions of FOS on L.rhamnosus colonization in different parts of mice intestine are still unclear.The study results indicated that the specific bands of enterobacterial repetitive intergenic consensus polymerase chain reaction(ERIC-PCR)in the L.rhamnosus(LR)group significantly increased at 7 days.Although the number of bands was similar to the natural recovery(NR)group,the brightness of few bands significantly enhanced in the later stage of recovery.Besides,Southern-blot maps showed strong signals,indicating that the ERIC-PCR fingerprint could accurately reflect the changes in the mouse gut microbiota diversity.Further,the high-throughput results confirmed that the Lactobacillus and Akkermansia had different changes at different periods,but all of them showed an upward trend,while the Klebsiella were inhibited,thereby maintaining the intestinal microecology balance.Moreover,FOS exerted a positive effect on L.rhamnosus colonization in the gut. 展开更多
关键词 Intestinal microbiota SYNBIOTICS FRUCTOOLIGOSACCHARIDES lactobacillus rhamnosus
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Lactobacillus rhamnosus GR-1 attenuates foodborne Bacillus cereus-induced NLRP3 inflammasome activity in bovine mammary epithelial cells by protecting intercellular tight junctions 被引量:5
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作者 Qiang Shan Ning Liu +3 位作者 Xue Wang Yaohong Zhu Jinhua Yin Jiufeng Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第1期307-321,共15页
Background:Bacillus cereus is an important pathogen that causes human food poisoning,specifically diarrhea and vomiting.B.cereus can also induce mastitis in dairy cows and has a strong survival ability in milk,as it c... Background:Bacillus cereus is an important pathogen that causes human food poisoning,specifically diarrhea and vomiting.B.cereus can also induce mastitis in dairy cows and has a strong survival ability in milk,as it cannot be inactivated by high-temperature short-time pasteurization.Therefore,B.cereus can enter the market through pasteurized milk and other dairy products,imposing enormous hidden dangers on food safety and human health.Results:In this study,B.cereus 2101(BC)was isolated from milk samples of cows with mastitis.BC grew rapidly with strong hemolysis,making it difficult to prevent mastitis and ensure food security.MAC-T cells were treated with BC and/or Lactobacillus rhamnosus GR-1(LGR-1).Pretreatment with LGR-1 protected the integrity of tight junctions and the expression of zonula occludens-1(ZO-1)and occludin destroyed by BC.Furthermore,LGR-1 pretreatment reduced the expression of NOD-like receptor family member pyrin domain-containing protein 3(NLRP3),caspase recruitment and activation domain(ASC),Caspase-1 p20,gasdermin D(GSDMD)p30,inflammatory factors(interleukin(IL)-1βand IL-18),and cell death induced by BC.Moreover,LGR-1 pretreatment reduced NLRP3 inflammasome activity and increased expressions of ZO-1 and occludin induced by lipopolysaccharides(LPS)+ATP stimulation.MAC-T cells were transfected with NLRP3 si RNA or MCC950 and/or treated with BC and/or LGR-1.NLRP3-si RNA transfection and MCC950 attenuated BC-induced NLRP3 inflammasome activity.Expression of inflammatory cytokines and cell death suggested that the inflammatory pathway might play an important role in the induction of the NLRP3 inflammasome by BC and the protection of LGR-1.Conclusions:These results suggest that LGR-1 might be a probiotic alternative to antibiotics and could be administered to prevent mastitis in dairy cows,thus ensuring food security. 展开更多
关键词 Bacillus cereus Intercellular tight junctions lactobacillus rhamnosus GR-1 NLRP3 inflammasome
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Exopolysaccharides of Lactobacillus rhamnosus GG ameliorate Salmonella typhimurium-induced intestinal inflammation via the TLR4/NF-κB/MAPK pathway 被引量:1
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作者 Jinze Li Qiuke Li +4 位作者 Qianhui Wu Nan Gao Zhihua Wang Yang Yang Anshan Shan 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第4期1501-1513,共13页
Background Salmonella typhimurium(S.T),as an important foodborne bacterial pathogen,can cause diarrhea and gastroenteritis in humans and animals.Numerous studies have confirmed that exopolysaccharides(EPSs)have variou... Background Salmonella typhimurium(S.T),as an important foodborne bacterial pathogen,can cause diarrhea and gastroenteritis in humans and animals.Numerous studies have confirmed that exopolysaccharides(EPSs)have various biological functions,but the mechanism through which EPSs improve the immunity of animals against the invasion of pathogenic bacteria is unclear.Here,we explored the protective effect of EPSs of Lactobacillus rhamnosus GG(LGG)on the S.T-infected intestine.Methods Mice received adequate food and drinking water for one week before the start of the experiment.After 7 d of prefeeding,2×108 CFU/mL S.T solution and an equivalent volume of saline(control group)were given orally for 1 d.On the fourth day,the mice were treated with 0.5 mg/mL EPSs,1.0 mg/mL EPSs,2.0 mg/mL EPSs,or 2.0 mg/mL penicillin for 7 d.Finally,the body and relative organ weight,histological staining,and the levels of antioxidant enzyme activity and inflammatory cytokines were determined.Results The S.T-infected mice exhibited symptoms of decreased appetite,somnolence,diarrhea and flagging spirit.Treatment with EPSs and penicillin improved the weight loss of the mice,and the high dose of EPSs showed the best therapeutic effect.EPSs significantly ameliorated S.T-induced ileal injury in mice.High-dose EPSs were more effective than penicillin for alleviating ileal oxidative damage induced by S.T.The mRNA levels of inflammatory cytokines in the ileum of mice showed that the regulatory effects of EPSs on inflammatory cytokines were better than those of penicillin.EPSs could inhibit the expression and activation of key proteins of the TLR4/NF-κB/MAPK pathway and thereby suppress the level of S.T-induced ileal inflammation.Conclusions EPSs attenuate S.T-induced immune responses by inhibiting the expression of key proteins in the TLR4/NF-κB/MAPK signaling pathway.Moreover,EPSs could promote bacterial aggregation into clusters,which may be a potential strategy for reducing the bacterial invasion of intestinal epithelial cells. 展开更多
关键词 EXOPOLYSACCHARIDES lactobacillus rhamnosus Salmonella typhimurium TLR4/NF-κB/MAPK
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Lactobacillus plantarum A3 attenuates ulcerative colitis by modulating gut microbiota and metabolism
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作者 Songkang Qin Yingli Wang +4 位作者 Mengjie Yang Pengpeng Wang Mudassar Iqbal Jinquan Li Yaoqin Shen 《Animal Diseases》 CAS 2023年第4期299-316,共18页
Antibiotics are widely used to treat various diseases.However,growing evidence indicates that antibiotic therapy in human life increases the incidence of inflammatory bowel disease(IBD).Therefore,we need appropriate m... Antibiotics are widely used to treat various diseases.However,growing evidence indicates that antibiotic therapy in human life increases the incidence of inflammatory bowel disease(IBD).Therefore,we need appropriate methods to reduce the incidence or symptoms of IBD.In this study,we used lincomycin hydrochloride to construct a gut micro-bial dysbiosis model in mice,and then,constructed an ulcerative colitis(UC)model.Meanwhile,we used Lactobacillus plantarum A3 from equine to treat UC in mice with gut microbial dysbiosis.The results showed that lincomycin hydro-chloride had little effect on the small gut microbiota in mice,but had a more destructive effect on the large intestin.Lactobaillus plantarum A3 alleviated the symptoms of UC in mice,which was reflected in its significantly reduced spleen index and disease activity index(DAl)(p<0.05),inhibited the shortening of colon and alleviated the invasion of inflammatory cells in the colon.Moreover,we found that it played a mitigatory role by inhibiting oxidative stress and regulating inflammatory cytokines in mice.At the same time,it restored the diversity and composition of the colonic microbiota and significantly increased the abundance of beneficial bacteria such as Blautia and Akkermansia(p<0.05);Notably,it significantly increased the concentrations of arachidonoyl ethanolamide phosphate(AEA-P)and cortisone(p<0.05)which have analgesic and anti-inflammatory effects.In conclusion,our study found that Lactobacillus plan-tarum A3 has the potential to regulate UC in mice with gut microbial dysbiosis. 展开更多
关键词 ANTIBIOTICS Gut microbiota lactobacillus plantarum DSS Ulcerative colitis Untargeted metabolomics
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Integrative proteomic-transcriptomic analysis revealed the lifestyles of Lactobacillus paracasei H4-11 and Kluyveromyces marxianus L1-1 under co-cultivation conditions
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作者 Na Liu Likang Qin +2 位作者 Haiying Zeng Anyan Wen Song Miao 《Food Science and Human Wellness》 SCIE CSCD 2023年第6期2195-2210,共16页
Compared with the rice-acid soup inoculated with single starter,the synergistically intensifi ed rice-acid soup inoculated with Lactobacillus paracasei H4-11(L.paracasei H4-11)and Kluyveromyces marxianus L1-1(K.marxia... Compared with the rice-acid soup inoculated with single starter,the synergistically intensifi ed rice-acid soup inoculated with Lactobacillus paracasei H4-11(L.paracasei H4-11)and Kluyveromyces marxianus L1-1(K.marxianus L1-1)contained more fl avor compounds.Organic acids mainly included L-lactic acid and the main volatile fl avor component was ethyl acetate.Moreover,the signal intensity of astringency and bitterness and the total concentration of volatile sulfur compounds were reduced.The combined analysis results of RNA sequencing(RNA-seq)technology and 4D label-free quantitative(4D LFQ)proteomics explained the fl avor formation pathways in rice-acid soup inoculated with L.paracasei H4-11 and K.marxianus L1-1.In L.paracasei H4-11,L-lactate dehydrogenase,phosphoglucomutase,acetate kinase,alcohol dehydrogenase and acetyl-CoA were up-regulated and D-lactate dehydrogenase and N-Acetyltransferase were down-regulated.In K.marxianus L1-1,Acetyl-CoA,acetaldehyde dehydrogenase,acyl-coenzyme A,N-acetyltransferase,and L-lactate dehydrogenase were up-regulated and hexokinase,alcohol dehydrogenase,and alcohol O-acetyltransferase were down-regulated.The above up-regulation and down-regulation synergistically promoted the formation of characteristic fl avor compounds(mainly L-lactic acid and ethyl acetate).Enzyme-linked immunoassay(ELISA)and parallel reaction monitoring(PRM)quantitative analysis respectively verifi ed that 5 key metabolic enzymes and 27 proteins in L.paracasei H4-11 and K.marxianus L1-1 were associated with the characteristic fl avor of rice-acid soup,as confi rmed by the quantitative results of 4D LFQ. 展开更多
关键词 lactobacillus paracasei Kluyveromyces marxianus Proteomic-transcriptomic Lifestyles Rice-acid soup
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Identification of Lactobacillus and Bifidobacterium 16S rRNA Gene in Breast Milk of Some Healthy Women in Kinshasa (DR Congo)
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作者 Olivier Nzingula Junior Disashi +3 位作者 José Mulwahali Pius Kabututu Paola Biakas Justin Masumu 《Journal of Biosciences and Medicines》 CAS 2023年第2期275-286,共12页
Breast milk is important for infant health. Some of its benefits are due to the presence of a specific population of bacteria in the microflora. However, the microbiome of breast milk is influenced by many parameters ... Breast milk is important for infant health. Some of its benefits are due to the presence of a specific population of bacteria in the microflora. However, the microbiome of breast milk is influenced by many parameters such as maternal diet, breastfeeding and geographic location. Culture and non-culture methods have been used in studies of this bacterial population worldwide. But in the DR Congo, there was no study reporting the use of culture-independent techniques to characterize the bacterial diversity of human milk. The aim of this study was to identify the bacterial 16S rRNA gene from two genera Lactobacillus and Bifidobacterium. The 16S rRNA gene was also identified from four species: Lactobacillus reuteri, Lactobacillus rhamnosus, Bifidobacterium longum and Bifidobacterium lactis. This analytical cross-sectional study was conducted in Kinshasa. Breast milk from some healthy women was collected from February 2 to 28, 2018. A culture-independent protocol using the classical polymerase chain reaction (PCR) was used to identify the bacterial 16S rRNA gene. The 68 samples of breast milk were collected in a sterile condition. The bacteria-specific ribosomal gene 16S rRNA was detected in 91.18% of Lactobacillus and 32.35% of Bifidobacterium at genus level. At of species level, only Lactobacillus reuteri 16S rRNA gene was identified in 89.71%. The 16S rRNA gene from the other species could not be amplified. There was also an association between educational level and the presence of Bifidobacterium and Lactobacillus 16S rRNA genes in the breast milk (p = 0.008*, p Conclusion: This study demonstrates the presence of the bacterial 16S rRNA gene from Lactobacillus and Bifidobacterium in breast milk at the genus and Lactobacillus reuteri at species level. A further study on the diet, use of antibiotics during pregnancy and lactation practice will provide a better understanding of the microflora of breast milk. 展开更多
关键词 16S rRNA PCR lactobacillus BIFIDOBACTERIUM Breast Milk
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Mutation Breeding of Lactobacillus and Its Application in Yoghurt
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作者 Xin LI Shiyun TANG +3 位作者 Fengrong TIAN Bing ZHANG Shijun FU Shijin GUO 《Agricultural Biotechnology》 CAS 2023年第1期31-34,共4页
With the gradual improvement of the quality of people’s life, the disorder of lipid metabolism caused by high-fat diet has become the main reason that affects the health of our people. Hyperlipidemia, hypertension an... With the gradual improvement of the quality of people’s life, the disorder of lipid metabolism caused by high-fat diet has become the main reason that affects the health of our people. Hyperlipidemia, hypertension and hyperglycemia have the effect of causing a large number of serious complications, and are also the main factors of cardiovascular disease. Lactic acid bacteria can avoid hyperlipidemia by inhibiting the excessive growth of harmful intestinal bacteria, maintaining the balance of intestinal microecology, absorbing intestinal cholesterol and regulating lipid metabolism. Therefore, this paper focused on the mutation and breeding of Lactobacillus, and analyzed its application in yoghurt, aiming to provide reference for relevant researchers. 展开更多
关键词 lactobacillus Mutation breeding YOGURT APPLICATION
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The Latest Research Progress on Application of Lactobacillus acidophilus
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作者 Jianxin Wen Xinyu Ma Yanli Liu 《Advances in Bioscience and Biotechnology》 2023年第6期298-307,共10页
Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of so... Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of some intestinal pathogenic bacteria, thereby regulating Intestinal flora, strengthen the absorption and utilization of feed nutrients, improve resistance, and promote animal growth, so it is widely used in livestock and poultry production. This article summarizes the latest research progress and application of Lactobacillus acidophilus, and looks forward to the future development direction in order to provide a theoretical basis for its future research. 展开更多
关键词 lactobacillus acidophilus PROBIOTICS Immune Function Non-Antibiotic Culture Microecological Preparation
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