利用传统培养法和变性梯度凝胶电泳技术(PCR-DGGE)评价Lactobacillus plantarum WCFS1对小鼠肠道微生物数量和种类的影响。结果表明,乳酸杆菌数量在灌胃期和恢复期较空白期显著升高(P<0.05),而肠杆菌数量在灌胃期和恢复期较空白期显...利用传统培养法和变性梯度凝胶电泳技术(PCR-DGGE)评价Lactobacillus plantarum WCFS1对小鼠肠道微生物数量和种类的影响。结果表明,乳酸杆菌数量在灌胃期和恢复期较空白期显著升高(P<0.05),而肠杆菌数量在灌胃期和恢复期较空白期显著下降(P<0.01);DGGE图谱及多样性分析显示小鼠灌胃期间菌群多样性显著提高(P<0.01),但在停止灌胃7d后与空白期相比无显著性差异(P>0.05)。此外,DGGE条带测序表明Lacto-bacillus plantarum WCFS1在灌胃期间可在大部分小鼠肠道内占据优势地位。展开更多
Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver o...Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver of high-fat diet(HFD) induced obese rats.Methods Three groups of animal models were established. Changes in mi RNA expression in the liver of each group were analyzed by microarray and RT-q PCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma(Hep G2) cells were used as a model to validate the test.Results LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride(TG) and total cholesterol(TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten mi RNAs and downregulation of five mi RNAs in the liver. Downregulation of mi R-34 a and mi R-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes(KEGG)pathway analysis showed that possible target genes of the deregulated mi RNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways.Conclusion These results demonstrate that LFBE might regulate the expression of mi RNAs in order to inhibit obesity and fatty liver.展开更多
文摘利用传统培养法和变性梯度凝胶电泳技术(PCR-DGGE)评价Lactobacillus plantarum WCFS1对小鼠肠道微生物数量和种类的影响。结果表明,乳酸杆菌数量在灌胃期和恢复期较空白期显著升高(P<0.05),而肠杆菌数量在灌胃期和恢复期较空白期显著下降(P<0.01);DGGE图谱及多样性分析显示小鼠灌胃期间菌群多样性显著提高(P<0.01),但在停止灌胃7d后与空白期相比无显著性差异(P>0.05)。此外,DGGE条带测序表明Lacto-bacillus plantarum WCFS1在灌胃期间可在大部分小鼠肠道内占据优势地位。
基金supported by the priority academic program development of Jiangsu Higher Education institutionsthe National Natural Science Foundation of China [31801538]
文摘Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver of high-fat diet(HFD) induced obese rats.Methods Three groups of animal models were established. Changes in mi RNA expression in the liver of each group were analyzed by microarray and RT-q PCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma(Hep G2) cells were used as a model to validate the test.Results LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride(TG) and total cholesterol(TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten mi RNAs and downregulation of five mi RNAs in the liver. Downregulation of mi R-34 a and mi R-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes(KEGG)pathway analysis showed that possible target genes of the deregulated mi RNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways.Conclusion These results demonstrate that LFBE might regulate the expression of mi RNAs in order to inhibit obesity and fatty liver.
