苦荬菜早熟(Lactuca indica L.)品种选育初报

苦荚菜(Lactuca indica L.)为菊科蒿苣属一年生青刈饲料作物。1977年由河北省引入我区种植,表现高产、品质优良、适口性好。在呼市地区一年可刈制3～4次,亩产鲜草万斤左右。蛋旦质含量与苜蓿相近,脂肪含量却较高,纤维素含量低,是猪、禽优良的青饲料。在呼市地区青饲期从六月下旬开始一直可延续至十月中下旬、所以深受群众欢迎。但是,苦荬菜在开始引入内蒙种植时,不能结实。其主要原因是:与引种地相比。

TDZ对莴苣(Lactuca sativa L.)器官发生及乙烯形成的影响

为了研究TDZ在植物器官发生中的生理作用，用不同浓度的TDZ替代MS中的BA，发现仅用1／50BA浓度（0．1mg／L）的TDZ处理，已相当于用BA处理所得芽数，可见它具有很强的细胞分裂素活性；但是TDZ又能显著促进乙烯形成，较低浓度时抑制莴苣的器官发生，可能此时乙烯起着主导作用。

水分胁迫对芒果(Mangifera indica L.)幼叶细胞活性氧伤害的影响

对芒果进行水分干旱胁迫处理 .结果表明 ,水分胁迫使芒果幼叶的相对含水量 RWC( relative water content)和叶水势 ΨT 下降 .芒果幼叶的超氧离子 O- 2.产生速率随水分胁迫处理强度加大而增加 .丙二醛 MDA( malondialdehyde)含量的变化趋势与 O- 2.产生速率的变化趋势相似 ,超氧歧化酶 SOD( superoxide dismutace) ,过氧化物酶 POD( peroxidase)和过氧化氢酶 CAT( catalase)的活性水平也与 O- 2.的变化相一致 ,细胞相对质膜透性加大 .说明芒果幼叶的水分胁迫损伤 ,是由 O- 2.引发的膜脂过氧化 ,致使 MDA含量增加 。

嗜酸乳杆菌发酵罗望子(Tamarindus indica L.)饮料的研制

以营养丰富的野生果树资源———罗望子为原料,利用浸提法制取原果汁,并对原汁进行预处理,得到适合嗜酸乳杆菌生长繁殖的罗望子果汁,通过发酵制得新型的果汁发酵饮料———罗望子活性乳酸菌饮料,并对其制作工艺进行了探讨。该活性乳酸菌制品营养丰富、芳香浓郁、口感怡人,饮料中嗜酸乳杆菌含量达1.0×108个/mL。

石莼(Ulva lactuca L.)营养成分及多糖组成分析

为使人们对石莼的食用价值有更加深入的了解,扩展其消费市场,促进石莼资源的开发及高值化利用,本文对石莼的主要营养成分、氨基酸组成、部分微量元素含量进行了测定,同时对其主要活性成分石莼多糖进行了红外光谱与单糖组成分析。结果表明,石莼中蛋白质、碳水化合物、粗纤维、脂肪、灰分含量分别为(%):22.03、38.00、8.77、1.11、30.09,富含人体必需的Ca、Mg、Fe等微量元素,氨基酸种类齐全且必需氨基酸含量高达5.90 g/100 g;经红外光谱与高效液相色谱分析表明,从石莼提取出的石莼多糖的主要单糖组成为鼠李糖(45.23%)、木糖(26.04%)和葡萄糖(23.73%),且有糖醛酸与硫酸基存在。

Selenium distribution and nitrate metabolism in hydroponic lettuce(Lactuca sativa L.): Effects of selenium forms and light spectra

A deficiency in selenium(Se) in the human diet is a worldwide problem. The intake of Se-rich vegetables can be a safe way to combat Se deficiency for humans. However, most leafy vegetables can accumulate a high content of nitrates, which poses a potential threat to human health. Light is an important environmental factor that regulates the uptake and distribution of mineral elements and nitrogen metabolism in plants. However, the effects of Se forms and light conditions, especially light spectra, on the uptake and translocation of Se and on nitrate reduction are poorly understood. In this study, lettuce(Lactuca sativa L.) was treated with exogenous Se applied as selenate(10 mmol L^-1) and selenite(0.5 mmol L^-1) and grown under five different light spectra: fluorescent light(FL), monochromatic red LED light(R), monochromatic blue LED light(B), and mixed red and blue LED light with a red to blue light ratio at 4(R/B=4), 8(R/B=8), and 12(R/B=12), respectively. The effects of light spectra and Se forms on plant growth, photosynthetic performance, Se accumulation and nitrate reduction were investigated. The results showed that the light spectra and Se forms had significant interactions for plant growth, foliar Se accumulation and nitrate reduction. The Se concentration and nitrate content in the leaves were negatively correlated with the percentage of red light from the light sources. Compared to Se applied as selenite, exogenous Se applied as selenate was more effective in reducing nitrate via promoting nitrate reductase and glutamate synthase activities. The lowest nitrate content and highest plant biomass were observed under R/B=8 for both the selenate and selenite treatments. The significant effect of the light spectra on the root concentration factor and translocation factor of Se resulted in marked variations in the Se concentrations in the roots and leaves. Compared with FL, red and blue LED light led to significant decreases in the foliar Se concentration. The results from this study suggest that the light spectra can contribute to Se distribution and accumulation to produce vegetables with better food quality.

Quantitative Trait Loci for Grain Chalkiness and Endosperm Transparency Detected in Three Recombinant Inbred Line Populations of Indica Rice

Quantitative trait loci（QTL） for percentage of chalky grain,degree of chalkiness,and endosperm transparency were detected using 3 recombinant inbred line populations derived from crosses between parental lines of commercial three-line hybrids of indica rice.Two of the populations showed great variations on heading date,and the other had a short range of heading date variation.A total of 40 QTLs were detected and fell into 15 regions of 10 chromosomes,of which 5 regions were detected for 1 or more same traits over different populations,2 were detected for different traits in different populations,3 were detected for 2 or all the 3 traits in a single population,and 5 were detected for a single trait in a single population.Most of these QTLs have been reported previously,but a region located on the long arm of chromosome 10 showing significant effects in all the 3 populations has not been reported before.It was shown that a number of gene cloned,including the Wx and Alk for the physiochemical property of rice grain,and GW2,GS3 and GW5 for grain weight and grain size,could have played important roles for the genetic control of grain chalkiness in rice,but there are many more QTLs exerting stable effects for rice chalkiness over different genetic backgrounds.It is worth paying more attentions to these regions which harbor QTL such as the qPCG5.2/qDC5.2/qET5.2 and qPCG10/qDC10/qET10 detected in our study.Our results also showed that the use of segregating populations having high-uniform heading date could greatly increase the efficiency of the identification of QTL responsible for traits that are subjected to great environmental influence.

Two new eudesmane sesquiterpenes from Lactuca sativa var.anagustata L

Two new eudesmane sesquiterpene lactones were isolated from the stalk of Lactuca sativa vat anagustata L and their structures were elucidated by means of spectroscopic methods, including 2D NMR （1H-1H COSY, HMBC and NOESY） as 1β-O-β-D- glucopyranosyl-4α-hydroxyl-5α, 6β, 11βH-eudesma-12, 6α-olide （1） and 1β-hydroxyl-15-O-（p-methoxyphenylacetyl）-5α, 6β, 11 βH-eudesma-3-en- 12, 6a-olide （2）.

Effects of Priming in Combination with Fungicides on Germination and Infestation of Lettuce (Lactuca sativa L.)

Two seed lots of lettuce (Lactuca sativa L.) cv. Krlowa Majowych were primed in polyethylene glycol (PEG) 8000 at -1.25MPa for 7 days in dark at 20C. Two fungicide treatments combined with PEG were used to control fungi: 1) Funaben T(0.1%) and Apron 35 SD (0.02%) were added to -1.25 MPa PEG solution (PEG+F); 2) After seed priming and drying,Funaben T was mixed at 5 g kg-1 and Apron 35 SD at 1 g kg-1 of seeds (PEG then F dust). PEG, PEG+F and PEG then F dusttreatments reduced significantly thermodormancy and increased the seed germination at 35C in both lots. PEG then Fdust treatment had the best effect on reducing seed thermodormancy at 35C, and increased seed germination to 64.5% inlot 1 and 72.0% in lot 2 compared to 0 in untreated. PEG and PEG then F dust treatments have accelerated germinationspeed at 20 and 15C in both lots. There was a significant reduction in seed infestation in which the seeds were treatedwith PEG+F and PEG then F dust compared to untreated seeds and PEG primed seeds. PEG priming enhanced seedinfestation considerably by Alternaria alternata, Penicillium spp., Rhizopus spp. and Ulocladium spp. Mycologicalanalysis showed higher frequency of Alternaria alternata and Ulocladium spp. in untreated seeds at 20C. Priming incombination with fungicides significantly alleviated the infestation of fungi, especially Alternaria alternata infestation.

开发和利用我国中草药作为储粮保护剂的研究——2.苦楝(Melia azedarach L.)和使君子(Quisqualis indica L.)对绿豆象和赤拟谷盗的毒杀作用

本文报导了苦楝(Melia azedarach L.)及使君子(Quisqualis indica L.)对绿豆象幼虫、成虫和赤拟谷盗各虫态的毒杀作用。利用喷雾、点滴和拌粮等不同方法处理试虫,结果表明,使君子粉的不同溶剂提取物对绿豆象幼虫、成虫和赤拟谷盗各虫态均无明显毒杀作用。苦楝粉95％乙醇提取物对两种试虫亦无明显毒杀作用。而苦楝油的毒杀效果则比较明显,并对其进行了室内毒力测定。

A new coumarin from Wikstroemia indica (L.) C.A.Mey

A new coumarin was obtained from the stems and roots of Wikstroemia indica （L.） C. A. Mey. Its structure was elucidated as methyl 3-（2-hydroxy-4-（7-hydroxy-6-methoxy-2-oxo-2H-chromen-3-yloxy） phenyl） propanoate by spectroscopic methods.

UPLC-Q-TOF-MS/MS法分析栾樨叶的化学成分

采用超高效液相色谱-四极杆-飞行时间高分辨质谱(UPLC-Q-TOF-MS/MS)对栾樨叶的化学成分进行定性分析。栾樨叶提取液经Waters UPLC BEH C_(18)柱(2.1 mm×100 mm,1.7μm)分离,以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,正、负离子模式下采集得到基峰色谱图。通过质谱数据库、化合物质谱裂解规律,并结合相关文献及对照品的保留时间与质谱信息,从栾樨叶中共鉴别出52个化学成分,包括2个香豆素类,15个黄酮类,16个苯丙素类,5个生物碱类,5个脂肪酸类,3个有机酸类,3个氨基酸类,1个酚酸类和2个其他类化合物,其中23个成分为栾樨叶中首次报道,11个化合物经对照品验证。该方法准确、可靠、高效,适用于栾樨叶化学成分的快速鉴定,可为栾樨叶的药效机制研究及临床应用提供依据。

不同LED组合光质对莴苣灰霉病防御酶活性的影响

本试验在水培条件下,以LED红蓝光(红蓝光比例4∶1)为基础和对照,研究添加紫光、黄光、绿光组成的不同LED组合光质对叶用莴苣接种灰霉病菌后的病情指数以及接菌前后POD、PPO、PAL等相关防御酶活性的影响。结果表明,不同LED组合光质处理中,以添加紫光的LED红蓝紫处理最能降低莴苣灰霉病病情指数;防御酶活性上,红蓝光基础上添加紫光和黄光处理的POD、PAL、PPO、GLU活性较高,添加绿光、黄光处理的CHT活性较高。整体上看,在LED红蓝光基础上添加一定比例的紫光或黄光,不仅可以有效降低莴苣灰霉病的病情指数,还可以提高莴苣体内的防御酶活性。该结果可为莴苣生产中通过光环境控制来抑制灰霉病发生提供理论参考。

Effects of chilling tolerance induced by spermidine pretreatment on antioxidative activity,endogenous hormones and ultrastructure of indica-japonica hybrid rice seedlings

Spermidine（Spd） is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress conditions.Antioxidant activities,endogenous hormones and ultrastructure change under chilling stress were investigated in indica-japonica hybrid rice seedlings.12-d-old seedlings were subjected to exogenous Spd（1 mmol L^（-1）） and then a chilling stress（6℃,4 d） was induced,followed by a subsequent recovery（25℃,4 d）.Results showed that malondialdehyde（MDA） and proline content were enhanced significantly,whereas shoot fresh and dry weights decreased during chilling stress and after recovery;chlorophyll content of chilling-stressed seedlings increased slightly but declined after recovery;additionally,total soluble sugar,sucrose,fructose and starch contents increased significantly during chilling stress,and only soluble sugar and fructose contents were observed in increase after recovery;chilling stress-induced increases in superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT） activities,but declined after recovery,and the level of ascorbate peroxidase was lower during chilling stress and after recovery;however,endogenous indole-3-acetic acid（IAA）,zeatin riboside（ZR）,gibberellic acid（GA_3）,and abscisic acid（ABA） levels were induced decreased compared with Spd pretreatment.The microscopic analysis revealed that chilling stress-induced destruction of the chloroplast envelope during chilling stress and increased the number of plastoglobuli along with aberrations in thylakoid membranes after recovery.In contrast,exogenous Spd protected rice seedlings from chilling-induced injuries in terms of lower malondialdehyde,proline and carbohydrates accumulation coupled with increased endogenous hormones metabolism.After recovery,Spd pretreatment chilling-exposed seedlings showed higher activities of antioxidant enzymes and normal physiological function of chloroplasts.These results suggest that Spd could promote effectively chilling tolerance which might be largely attributable to the integrity of cell structure and normal metabolism of endogenous hormones in indica-japonica hybrid rice seedlings.

Molecular Cloning and Sequence Analysis of C4H Gene of Mangifera indica L.

Cinnamate-4-hydroxylase( C4H) is a key enzyme in phenylpropanoid pathway in plants. Its activity and abundance directly affect the biosynthesis of flavonoids and aromatic compounds. In this study,degenerate primers were designed according to previously reported C4 H gene sequences to clone C4H cDNA sequence with 3'and 5'RACE-PCR from mango( Mangifera indica L). The full-length cD NA of M. indica C4H is 1 680 bp long. Its open reading frame( ORF)is 1 518 bp,encoding a protein of 505 amino acids with a predicted molecular weight of 58. 08 kDa. The isoelectric point of the predicted protein is 9. 52. Functional prediction showed that this gene is mainly located in mitochondria. In addition,the tertiary structure of the protein was built using SWISS-MODEL,and the results showed that the protein has three possible conformations. Phylogenetic analysis based on C4H protein sequences revealed that M. indica has a close genetic relationship with olive( Canarium album) and cocoa( Theobroma cacao). By analyzing the expression level of C4H gene in three colored mango cultivars,we found that that the expression level of C4 H gene in Guifei( with red peel) was the highest,and that in Guiqi( with green peel) was the lowest. The results provide a theoretical basis for studying the molecular mechanism of anthocyanin biosynthesis and C4H's impact on the color of mango fruit.

Unripe Fruit Extracts of <i>Mangifera indica L.</i>Protect against AGEs Formation, Melanogenesis and UVA-Induced Cell Damage

In this study, we explored the effects of unripe fruit extracts of Mangifera indica L. on the anti-aging activity in skin cells. Mangifera indica L. is a popular economical and medicinal plant with numerous health-beneficial properties. The aqueous extracts of unripe fruit of Mangifera indica L. were obtained and subjected to HPLC and NMR analyses for the identification of bioactive compounds. The anti-glycation effect of Mango unripe fruit extracts was monitored by in vitro model system of AGEs (Advanced glycation end products) formation. Mango unripe fruit extracts significantly inhibited the AGEs formation in a dose-dependent manner. Meanwhile, Mango unripe fruit extracts possessed a comparable efficiency to commercialized Kojic acids in the inhibition of melanogenesis in B16-F10 melanoma cells. The UVA-induced cell damages can be prevented and repaired by Mango unripe fruit extracts in skin fibroblast CCD-966SK. Compared to the untreated control, Mango unripe fruit extracts significantly increased the cell viability while being applied before (36%) or after (43%) UVA irradiation. These results verified the potential application of Mango unripe fruit extracts in the skin protection and recovery from UVA irradiation, as well as the suppression of AGEs formation and melanogenesis.

Construction of Lactuca sativa Plastid Transformation Vector and High-level Expression of gfp Gene in Escherichia coli

Using genomic DNA of bolting-tolerant lettuce as a template,flanking fragments of lettuce plastid rpo A gene were amplified and cloned by PCR. Targeting the sites of these two fragments,homologous recombinant fragments of exogenous gene were integrated to construct lettuce plastid expression vector p Brpo AGFP,which harbored the expression cassette Prrn-gfp-aad A-Tpsb A. The results showed that the amplified flanking fragments were 1.2 and 1.1 kb in size. After sequencing,restriction digestion,ligation and transformation,lettuce plastid expression vector containing expression cassette Prrn-gfp-aad A-Tpsb A was constructed and confirmed by SDS-PAGE electrophoresis. The results of SDS-PAGE electrophoresis indicated that gfp gene was efficiently expressed under the regulation of plasmid specific promoter Prrn and terminator Tpsb A. GFP accounted for 45. 6% of total soluble proteins; inclusion bodies accounted for 47.5 % of bacterial proteins,which reached relatively high expression levels. The construction of lettuce plastid expression vector p Brpo A-GFP laid a solid foundation for establishment of subsequent lettuce plastid transformation system and genetic improvement of lettuce using various functional genes.

Efficient Regeneration System for Genetic Transformation of Mulberry (<i>Morus indica</i>L. Cultivar S-36) Using <i>in Vitro</i>Derived Shoot Meristems

Shoot meristems used for the study were exercised from the in vitro regenerated shoots cultured on MS medium supplemented with 0.5 mg/L of BAP for multiplication. The sensitivity of the in vitro regenerated was studied using shoot meristems of 0.5 cm. Shoot meristems were cultured on medium containing 10-100 mg/l kanamycin to determine the concentration that was lethal for multiple shoot induction and root induction. The response of shoot multiplication decreased (66.2%-6.2%) as the concentration of kanamycin increased (10.0-70.0 mg/L) with complete inhibition of shoot proliferation at 100 mg/L kanamycin. The rooting phase was very sensitive to kanamycin compared to shoot multiplication. The percentage of shoots that rooted decreased (53.8%-4.8%) with increase in the concentration of kanamycin (10.0-70.0 mg/l) on IBA and 2,4-D supplemented medium. For transformation studies, the shoot tips that were infected with Agrobacterium strain were placed on selection medium containing MS medium with 0.5 mg/L BAP and 100 mg/L kanamycin and scored for the putative transformed shoots. An average of 62.2% of shoot tips developed shoot buds from the base and the shoots reached a length of 0.5-1.0 cm at the end of 30 days of culture on the selective medium in comparison to control which showed no response. An average of 66.7% of the regenerated plants showed GUS expression on selection medium where 43.2% and 65% of GUS expression was recorded in the leaves and callus. Leaves and callus induced from the controls did not show GUS activity. Stable integration of nptII gene with the genomic DNA from these transformed plants was confirmed through PCR analysis. Our result presents an efficient regeneration system using in vitro derived shoot meristems for Agrobacterium mediated gene transfer.

Occurrence Regularity of Fruit Physiological Disease Spongy Tissue in Zihua Mango(Mangifera indica L.)

[ Objective ] The aim was to study the occurrence regularity of fruit physiological disease spongy tissue in Zihua mango （Mangifera indica L. ）. [ Meth. od] Main features of disease symptoms of Zihua mango fruit spongy tissue were investigated from 2002 to 2005 ,and the correlation between the incidence of Zihua mango fruit spongy tissue and its external factors （ fruit maturity, fruit size and fruit yield per plant） was analyzed comprehensively. [Result] The main features of disease symptoms appeared depressed cavity in the middle or lower part of fruit, forming spongy-like cavity. Immature fruits basically had no incidence. The dis- ease began to appear before 10 d of maturity. The disease incidence rate had extremely positive correlation with fruit weight, fruit vertical diameter or cross diame- ter. [ Conclusion] The research provides reference for field diagnoses, identification, preharvest and postharvest uninjurous test of fruit physiological disease suonaw tissue.

Antimicrobial Activities of Seed Extracts of Mango (<i>Mangifera indica</i>L.)

Mangifera indica L. is a species of mango in the Anacardiaceae family. It is found in the wild in tropical regions and cultivated varieties have been introduced to other warm regions of the world. This present study aimed to investigate the in vitro antimicrobial activities of methanol and ethanol extracts of mango seed against 25 representatives gram positive, gram negative, acid fast bacteria and fungi. Mango fruit seed were extracted by Soxhlet using methanol and ethanol as solvents. The extracts were tested against the microorganisms using disc diffusion method at different concentrations: 5 mg/mL, 3.75 mg/mL, 3.125 mg/mL, 2.5 mg/mL, 1.875 mg/mL and 1.25 mg/mL). In vitro antibacterial activities of methanol and ethanol extracts of mango bulb showed inhibitions to tested organisms with variable inhibition zones. Except one organism (Rhodococcus equi), no resistance among the tested strains was shown. The mean zone of inhibition produced ranged between 5 mmand18 mmwith18 mm/Mycobacterium smegmatis showed the highest zone of inhibition. In most test strains comparable zones of inhibitions were noted for both methanol and ethanol extract. Candida albicans and Aspergilllus niger were both inhibited by the extracts. The methanol and ethanol extracts of mango seed showed good inhibitory effects against almost all tested strains. The inhibition zones produced by mango extract were less than those produced by standard positive control drug. This could be due to low diffusion rate of mango extract in agarose medium, a thing needed to be further investigated. The products are potential new antimicrobial therapy in the ethnopharmacology domain.