Objective: To investigate the role of laminins in the pathogensis of mesangial praliferalive glomeruonephritis (MsPGN ) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied...Objective: To investigate the role of laminins in the pathogensis of mesangial praliferalive glomeruonephritis (MsPGN ) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied by means of immunohistochemistry and in situ hybridization.Results: ① Protein of α1 chain and γ1 chain of laminin increased around the segments of proliferative mesangium. Increased expression of α2 and βl proteins was found in the segments with mesangial proliferation whereas the β2 chain expression decreased in these areas. ② The mRNA expression of αl,α2,β1 and γ1 increased to different degrees in glomeeruli with mesangial proliferation. But no difference was detected among Mild, Moderate, and Severe MsPGN. Conclusion:①The quantitative and qualitative alterations of laminin chains’ distribution were found in the measngial proliferative glomeruli. The proliferative mesougial cells were the origins of abnormal accumulation and expression of laminins.③ These changes may be the basis of the progresses of MsPGN.展开更多
To examine the effect of multi-glycoside of Tripterygium wilfordii Hook. f. (GTW) on proteinuria and acute glomerular immune lesion in experimental mesangial proliferative glomerulonephritis (MsPGN) induced by anti-Th...To examine the effect of multi-glycoside of Tripterygium wilfordii Hook. f. (GTW) on proteinuria and acute glomerular immune lesion in experimental mesangial proliferative glomerulonephritis (MsPGN) induced by anti-Thy1.1 monoclonal antibody (mAb) 1-22-3. The reversible model of MsPGN with anti-Thy1.1 mAb 1-22-3 was established. After 7 days of oral treatment with GTW (100 mg/kg per day) and vehicle (distilled water, 5 ml/kg per day), its effects on proteinuria, renal functions, mesangial morphological change, glomerular macrophage accumulation, and mRNA expressions of cytokines (PDGF-BB and MCP-1) were evaluated by light microscope (LM), immunofluorescence (IF), and reverse transcription polymerase chain reaction (RT-PCR). It was found that GTW ameliorated proteinuria (on day 3 and day 7), mesangial proliferation (total cell number, matrix expansion, α-smooth muscle actin expression, and collagen type Ⅰ expression) and macrophage accumulation (ED3^+) in experimental MsPGN. In addition, GTW significantly suppressed the increased mRNA expressions for MCP-1 (67.6% to control group, P<0.01) together with the tendency to reduce the expression of PDGF (24.44% to control group) on day 7. It is concluded that GTW can not only decrease proteinuria, but also ameliorate acute mesangial alterations and glomerular activated macrophage accumulation probably by reduction of cytokines. These data indicate that GTW is an effective agent for early MsPGN.展开更多
Objective:Rheumatoid nephropathy is one of the most severe extra-articular manifestations of rheumatoid arthritis(RA)associated with a very unfavorable prognosis.This study aimed to identify changes in renal function ...Objective:Rheumatoid nephropathy is one of the most severe extra-articular manifestations of rheumatoid arthritis(RA)associated with a very unfavorable prognosis.This study aimed to identify changes in renal function and morphological variations of kidney diseases in RA patients.Methods:The study enrolled patients(126 patients)between 18 and 55 years of age with a confirmed active RA of more than 12 months.Each patient underwent the following range of laboratory and instrumental research methods:general clinical analysis of blood and urine,performing urinalysis according to Nechiporenko method;determining daily proteinuria;determining the blood content of glucose,urea,creatinine,uric acid,total bilirubin,liver transaminase level,ionogram,lipidogram,and coagulogram;determining the blood content of rheumatoid factor,anti-streptolysin O,and C-reactive protein;and X-ray of the joints of hands and feet.Renal function was examined by estimating glomerular filtration rate,tubular reabsorption index,and renal functional reserve.For studying the morphological changes in the kidneys under ultrasound examination,renal biopsy was performed in 31 patients with RA with urinary syndrome(proteinuria more than 0.3 g per day and hematuria).Results:Nephropathy in RA is characterized by impaired renal function and manifested by an increased blood creatinine and a decrease in glomerular filtration rate and renal functional reserve.Among morphological variations of nephropathy at RA,mesangial proliferative glomerulonephritis prevails,accounting for 48.4%of patients.Other disorders include the secondary amyloidosis(29.0%of patients),tubulointerstitial nephritis(16.1%),membranous glomerulonephritis(3.2%),and focal-segmental glomerulosclerosis(3.2%).Conclusion:Kidney damage is a common systemic manifestation of RA with a long and active course,a major nephropathy trigger.展开更多
基金Supported by Grant from the Natural Science Foundation of Education Committee of Jiangsu Province(96047)
文摘Objective: To investigate the role of laminins in the pathogensis of mesangial praliferalive glomeruonephritis (MsPGN ) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied by means of immunohistochemistry and in situ hybridization.Results: ① Protein of α1 chain and γ1 chain of laminin increased around the segments of proliferative mesangium. Increased expression of α2 and βl proteins was found in the segments with mesangial proliferation whereas the β2 chain expression decreased in these areas. ② The mRNA expression of αl,α2,β1 and γ1 increased to different degrees in glomeeruli with mesangial proliferation. But no difference was detected among Mild, Moderate, and Severe MsPGN. Conclusion:①The quantitative and qualitative alterations of laminin chains’ distribution were found in the measngial proliferative glomeruli. The proliferative mesougial cells were the origins of abnormal accumulation and expression of laminins.③ These changes may be the basis of the progresses of MsPGN.
文摘To examine the effect of multi-glycoside of Tripterygium wilfordii Hook. f. (GTW) on proteinuria and acute glomerular immune lesion in experimental mesangial proliferative glomerulonephritis (MsPGN) induced by anti-Thy1.1 monoclonal antibody (mAb) 1-22-3. The reversible model of MsPGN with anti-Thy1.1 mAb 1-22-3 was established. After 7 days of oral treatment with GTW (100 mg/kg per day) and vehicle (distilled water, 5 ml/kg per day), its effects on proteinuria, renal functions, mesangial morphological change, glomerular macrophage accumulation, and mRNA expressions of cytokines (PDGF-BB and MCP-1) were evaluated by light microscope (LM), immunofluorescence (IF), and reverse transcription polymerase chain reaction (RT-PCR). It was found that GTW ameliorated proteinuria (on day 3 and day 7), mesangial proliferation (total cell number, matrix expansion, α-smooth muscle actin expression, and collagen type Ⅰ expression) and macrophage accumulation (ED3^+) in experimental MsPGN. In addition, GTW significantly suppressed the increased mRNA expressions for MCP-1 (67.6% to control group, P<0.01) together with the tendency to reduce the expression of PDGF (24.44% to control group) on day 7. It is concluded that GTW can not only decrease proteinuria, but also ameliorate acute mesangial alterations and glomerular activated macrophage accumulation probably by reduction of cytokines. These data indicate that GTW is an effective agent for early MsPGN.
文摘Objective:Rheumatoid nephropathy is one of the most severe extra-articular manifestations of rheumatoid arthritis(RA)associated with a very unfavorable prognosis.This study aimed to identify changes in renal function and morphological variations of kidney diseases in RA patients.Methods:The study enrolled patients(126 patients)between 18 and 55 years of age with a confirmed active RA of more than 12 months.Each patient underwent the following range of laboratory and instrumental research methods:general clinical analysis of blood and urine,performing urinalysis according to Nechiporenko method;determining daily proteinuria;determining the blood content of glucose,urea,creatinine,uric acid,total bilirubin,liver transaminase level,ionogram,lipidogram,and coagulogram;determining the blood content of rheumatoid factor,anti-streptolysin O,and C-reactive protein;and X-ray of the joints of hands and feet.Renal function was examined by estimating glomerular filtration rate,tubular reabsorption index,and renal functional reserve.For studying the morphological changes in the kidneys under ultrasound examination,renal biopsy was performed in 31 patients with RA with urinary syndrome(proteinuria more than 0.3 g per day and hematuria).Results:Nephropathy in RA is characterized by impaired renal function and manifested by an increased blood creatinine and a decrease in glomerular filtration rate and renal functional reserve.Among morphological variations of nephropathy at RA,mesangial proliferative glomerulonephritis prevails,accounting for 48.4%of patients.Other disorders include the secondary amyloidosis(29.0%of patients),tubulointerstitial nephritis(16.1%),membranous glomerulonephritis(3.2%),and focal-segmental glomerulosclerosis(3.2%).Conclusion:Kidney damage is a common systemic manifestation of RA with a long and active course,a major nephropathy trigger.
