[Objectives]This study was conducted to study the chemical constituents from Laportea bulbifera. [Methods] The 60% ethanol extract from L. bulbifera was isolated and purified by silica, Sephadex LH-20, ODS, semi-prepa...[Objectives]This study was conducted to study the chemical constituents from Laportea bulbifera. [Methods] The 60% ethanol extract from L. bulbifera was isolated and purified by silica, Sephadex LH-20, ODS, semi-preparative HPLC and recrystallization. Their chemical structures were elucidated by physicochemical properties and spectroscopic methods. [Results] These compounds were determined as kaempferol-3-O-α-L-(3-O-acetyl)-rhamnoside-7-O-α-L-rhamnoside(1), sutchuenoside A(2), kaempferol-3-O-[β-D-glucopyranosyl-(1→3)]-α-L-(4-O-acetyl)-rhamnopyranoside-7-O-α-L-rhamnopyranoside(3). Compounds 1-3 are isolated from genus Laportea for the first time. Compound 3 is a new compound. [Conclusions] This study lays a foundation for improving the quality standard of L. bulbifera and the development and utilization of its resources.展开更多
[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were ...[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were identified by microscopy. [Results] The main microscopic identification characteristics of the tissue structures of L. bulbifera leaf and stem at different positions and L. bulbifera powder were determined. [Conclusions] The microscopic identification results are characteristic, and could serve as the identification basis of L. bulbifera. These results could provide references for the quality control of L. bulbifera.展开更多
Laportea bulbifera(Sieb.et Zucc.)Wedd.is a perennial herb of Laportea in Urticaceae.At present,more than 90 compounds have been isolated from L.bulbifera,mainly including flavonoids,coumarins,phenolic acids,esters,ket...Laportea bulbifera(Sieb.et Zucc.)Wedd.is a perennial herb of Laportea in Urticaceae.At present,more than 90 compounds have been isolated from L.bulbifera,mainly including flavonoids,coumarins,phenolic acids,esters,ketones,phenylpropanoids and steroids,which have anti-inflammatory and analgesic,immunosuppressive,anti-oxidant,hypoglycemic and lipid-lowering pharmacological effects.This article summarized the research progress of the pharmacological effects and the chemical constituents of L.bulbifera in recent years,hoping to provide references for its further research and development.展开更多
[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify an...[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture, total ash and extract contents. [Results] According to the microscopic results, the stem transection is round and has five longitudinal edges at which the cell wall is obviously thickened. Bristles can be observed. A series of collenchyma cells are arranged closely inside the epidermis. There are several rows of parenchyma cells. The vascular bundles are of different sizes;and the ray part is wide, and at the junction of the ray and the cortex, there are often scattered secretory cells. The center is a broad medulla. The powder is brown. There are many starch grains. The bristles are unicelluar, and have warty protrusions and expanded base. The fibers are bundled, and the cells are large. The epidermal cells are in the shape of an elongated spindle, in a mosaic arrangement, and the cell wall is slightly thickened. From the thin layer identification chromatograms of tested L. bulbifera, it can be seen that spots were observed at the same positions as the control medicinal material, and the durability of the method was good. The extract content should not be less than 12.00% by hot extraction with water as solvent, and not be less than 3.00% by cold extraction with ethanol as solvent. The moisture content should not be more than 16.50%. The total ash content should not be more than 14.00%. The acid-insoluble ash content should not be more than 2.50%. [Conclusions] The microscopic identification results are reliable and can be used as the identification basis of L. bulbifera. The thin layer identification method and the test results can provide a basis for quality control of L. bulbifera.展开更多
Papua is rich in biodiversity. The indigenous Papuans utilize most of endemic plants as food, board and medicines. One of the plants used as medicine is La-portea, sp, that locally known as Daun Gatal. The plant howev...Papua is rich in biodiversity. The indigenous Papuans utilize most of endemic plants as food, board and medicines. One of the plants used as medicine is La-portea, sp, that locally known as Daun Gatal. The plant however, has not been developed commercially;this might due to lack of scientific providence of medicine content in this plant. The aims of this research is to (1) explore the types of Laportea growing in Papua, (2) conduct bio molecular character, which observes phylogeny relationship or genetic morphology in order to obtain data on the number of species found in Manokwari and Nabire. Bio molecular analysis is performed by PCR. Genetic analysis in this study was conducted through several phases, as follow: (a) Isolation of genomic total, (b) amplification of genes that encoded Rbcl, (c) sequencing the nucleotide sequence of the gene Rbcl of Laportea, (d) phylogeny analysis. The results showed that the DNA quality was good enough to do the next analysis. Of the four samples, two obtained haplo types are Haplo Type 1 (DT.NBX.H, DT.NBX.U and DR.MKW.H) and Haplo Type 2 (DR.NBX.L). The results of the analysis of phylogeny trees showed samples at 1 in the same haplo type, clade sequences of the genus Dendrocnide spp, Discocnide, and Laportea, while the sample at 2 in the same haplotype clade with Laportea interupta, Laportea ruderalis and Urera sp.展开更多
基金Supported by Guangxi University Scientific Research Project of Colleges and Universities in Guangxi (NO. YB2014192)Key R&D Program of Guangxi (GK AB19110027)High-level Innovation Team and Outstanding Scholar Program of Colleges and Universities in Guangxi:Basic and Clinical Innovation Team of Zhuang Medicine (GJR[2014]07)。
文摘[Objectives]This study was conducted to study the chemical constituents from Laportea bulbifera. [Methods] The 60% ethanol extract from L. bulbifera was isolated and purified by silica, Sephadex LH-20, ODS, semi-preparative HPLC and recrystallization. Their chemical structures were elucidated by physicochemical properties and spectroscopic methods. [Results] These compounds were determined as kaempferol-3-O-α-L-(3-O-acetyl)-rhamnoside-7-O-α-L-rhamnoside(1), sutchuenoside A(2), kaempferol-3-O-[β-D-glucopyranosyl-(1→3)]-α-L-(4-O-acetyl)-rhamnopyranoside-7-O-α-L-rhamnopyranoside(3). Compounds 1-3 are isolated from genus Laportea for the first time. Compound 3 is a new compound. [Conclusions] This study lays a foundation for improving the quality standard of L. bulbifera and the development and utilization of its resources.
基金Supported by Guangxi Colleges and Universities Scientific Research Funding Project(NO.YB2014192)Guangxi Zhuang Medicine Quality Evaluation and Standard Research Project(NO.MZY2013023)+2 种基金Guangxi Scientific Research and Technological Development Program(GKG14124002-11-1)High-level-innovation Team and Outstanding Scholar Project of Guangxi Higher Education Institutes-Zhuang Medicine Basic and Clinical Innovation Team(GJR [2014]07)College Students'Innovation and Enterpreneurship Training Program of Guangxi University of Chinese Medicine(NO.2017DXS35)
文摘[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were identified by microscopy. [Results] The main microscopic identification characteristics of the tissue structures of L. bulbifera leaf and stem at different positions and L. bulbifera powder were determined. [Conclusions] The microscopic identification results are characteristic, and could serve as the identification basis of L. bulbifera. These results could provide references for the quality control of L. bulbifera.
基金Research Fund of Guangxi Colleges and Universities(YB2014192)Guangxi Key R&D Program(GK AB19110027)High-level Innovation Team and Outstanding Scholars Project of Guangxi Colleges and Universities:Zhuang Medical Foundation and Clinical Innovation Team(GJR[2014]07).
文摘Laportea bulbifera(Sieb.et Zucc.)Wedd.is a perennial herb of Laportea in Urticaceae.At present,more than 90 compounds have been isolated from L.bulbifera,mainly including flavonoids,coumarins,phenolic acids,esters,ketones,phenylpropanoids and steroids,which have anti-inflammatory and analgesic,immunosuppressive,anti-oxidant,hypoglycemic and lipid-lowering pharmacological effects.This article summarized the research progress of the pharmacological effects and the chemical constituents of L.bulbifera in recent years,hoping to provide references for its further research and development.
基金Supported by Study on Quality Evaluation and Standards of Guangxi Yao Medicine(MZY2012015)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+2 种基金Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)High-level-innovation Team and Outstanding Scholar Project of Guangxi Higher Education Institutions:Zhuang Medicine Foundation and Clinical Innovation Team(GJR[2014]07)Student Research Training Program of Guangxi University of Chinese Medicine(NO.2017DXS35).Wei WEI(1981-),male,P.R.China,experimentalist,devoted to research about Tradition Chinese medicine and ethnic drug
文摘[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture, total ash and extract contents. [Results] According to the microscopic results, the stem transection is round and has five longitudinal edges at which the cell wall is obviously thickened. Bristles can be observed. A series of collenchyma cells are arranged closely inside the epidermis. There are several rows of parenchyma cells. The vascular bundles are of different sizes;and the ray part is wide, and at the junction of the ray and the cortex, there are often scattered secretory cells. The center is a broad medulla. The powder is brown. There are many starch grains. The bristles are unicelluar, and have warty protrusions and expanded base. The fibers are bundled, and the cells are large. The epidermal cells are in the shape of an elongated spindle, in a mosaic arrangement, and the cell wall is slightly thickened. From the thin layer identification chromatograms of tested L. bulbifera, it can be seen that spots were observed at the same positions as the control medicinal material, and the durability of the method was good. The extract content should not be less than 12.00% by hot extraction with water as solvent, and not be less than 3.00% by cold extraction with ethanol as solvent. The moisture content should not be more than 16.50%. The total ash content should not be more than 14.00%. The acid-insoluble ash content should not be more than 2.50%. [Conclusions] The microscopic identification results are reliable and can be used as the identification basis of L. bulbifera. The thin layer identification method and the test results can provide a basis for quality control of L. bulbifera.
文摘Papua is rich in biodiversity. The indigenous Papuans utilize most of endemic plants as food, board and medicines. One of the plants used as medicine is La-portea, sp, that locally known as Daun Gatal. The plant however, has not been developed commercially;this might due to lack of scientific providence of medicine content in this plant. The aims of this research is to (1) explore the types of Laportea growing in Papua, (2) conduct bio molecular character, which observes phylogeny relationship or genetic morphology in order to obtain data on the number of species found in Manokwari and Nabire. Bio molecular analysis is performed by PCR. Genetic analysis in this study was conducted through several phases, as follow: (a) Isolation of genomic total, (b) amplification of genes that encoded Rbcl, (c) sequencing the nucleotide sequence of the gene Rbcl of Laportea, (d) phylogeny analysis. The results showed that the DNA quality was good enough to do the next analysis. Of the four samples, two obtained haplo types are Haplo Type 1 (DT.NBX.H, DT.NBX.U and DR.MKW.H) and Haplo Type 2 (DR.NBX.L). The results of the analysis of phylogeny trees showed samples at 1 in the same haplo type, clade sequences of the genus Dendrocnide spp, Discocnide, and Laportea, while the sample at 2 in the same haplotype clade with Laportea interupta, Laportea ruderalis and Urera sp.
