Low-pressure air plasma cleaning is an effective method for removing organic contaminants on large-aperture optical components in situ in the inertial confinement fusion facility.Chemical reactions play a significant ...Low-pressure air plasma cleaning is an effective method for removing organic contaminants on large-aperture optical components in situ in the inertial confinement fusion facility.Chemical reactions play a significant role in plasma cleaning,which is a complex process involving abundant bond cleavage and species generation.In this work,experiments and reactive molecular dynamics simulations were carried out to unravel the reaction mechanism between the benchmark organic contaminants of dibutyl phthalate and air plasma.The optical emission spectroscopy was used to study the overall evolution behaviors of excited molecular species and radical signals from air plasma as a reference to simulations.Detailed reaction pathways were revealed and characterized,and specific intermediate radicals and products were analyzed during experiments and simulation.The reactive species in the air plasma,such as O,HO_(2)and O_(3)radicals,played a crucial role in cleaving organic molecular structures.Together,our findings provide an atomic-level understanding of complex reaction processes of low-pressure air plasma cleaning mechanisms and are essential for its application in industrial plasma cleaning.展开更多
The safety and integrity requirements of aerospace composite structures necessitate real-time health monitoring throughout their service life.To this end,distributed optical fiber sensors utilizing back Rayleigh scatt...The safety and integrity requirements of aerospace composite structures necessitate real-time health monitoring throughout their service life.To this end,distributed optical fiber sensors utilizing back Rayleigh scattering have been extensively deployed in structural health monitoring due to their advantages,such as lightweight and ease of embedding.However,identifying the precise location of damage from the optical fiber signals remains a critical challenge.In this paper,a novel approach which namely Modified Sliding Window Principal Component Analysis(MSWPCA)was proposed to facilitate automatic damage identification and localization via distributed optical fiber sensors.The proposed method is able to extract signal characteristics interfered by measurement noise to improve the accuracy of damage detection.Specifically,we applied the MSWPCA method to monitor and analyze the debonding propagation process in honeycomb sandwich panel structures.Our findings demonstrate that the training model exhibits high precision in detecting the location and size of honeycomb debonding,thereby facilitating reliable and efficient online assessment of the structural health state.展开更多
Purpose: To establish the prevalence and severity of myopia among the Chinese Hong Kong students and to study the relationship between myopia and optical components.Methods;One thousand and seventy-five freshmen of th...Purpose: To establish the prevalence and severity of myopia among the Chinese Hong Kong students and to study the relationship between myopia and optical components.Methods;One thousand and seventy-five freshmen of the 1993-1994 academic year in the Chinese University of Hong Kong underwent the eye examination including evaluation of refractive error, keratometry, and A-scan ultrasonic biometry. The data were analyzed with the SPSS/PC+4. 01 statistical package. Results: The prevalence of myopia was 91. 7% with the mean refraction being -4. 00 ± 2. 64D in this young adult population. The statistical analyses demonstrated a significant correlation between refractive value and axial length of the globe (r=-0. 78), vitreous length (r=-0. 76), anterior chamber depth (r=-0. 33), lens thickness (r = 0. 13) and corneal curvature (r = 0. 19). Conclusion: The refractive status is mainly dependent on the axial length. In general, the higher the myopia was, the longer the eyeball, the deeper the anterior chamber,展开更多
Introduction The endothelial cells(ECs)lining every blood vessel wall constantly expose to the mechanical forces generated by the blood flow.The EC responses to these hemodynamic forces play a critical role in the hom...Introduction The endothelial cells(ECs)lining every blood vessel wall constantly expose to the mechanical forces generated by the blood flow.The EC responses to these hemodynamic forces play a critical role in the homeostasis of the circulatory system.In addition to forming a transport barrier between the blood and vessel wall,vascular ECs play important roles in regulating circulation functions.Besides biochemical stimuli,blood flow induced(hemodynamic)mechanical stimuli,such as shear stress,pressure and circumferential stretch,modulate EC morphology and functions by activating mechanosensors,signaling pathways,and gene and protein expressions.The EC responses to the hemodynamic forces(mechano-sensing and transduction)are critical to maintaining normal vascular functions.Failure in the mechano-sensing and transduction leads to serious vascular diseases including hypertension,atherosclerosis,aneurysms and thrombosis,to name a few[1].On the luminal surface of our blood vessels,there is a thin layer called endothelial surface glycocalyx(ESG)which consists of proteoglycans,glycosaminoglycans(GAGs)and glycoproteins.The GAGs in the ESG are heparan sulfate(HS),hyaluronic acid(HA),chondroitin sulfate(CS),and sialic acid(SA)[2].In order to play important roles in vascular functions,such as being a mechanosensor and transducer for the endothelial cells(ECs)to sense the blood flow,a molecular sieve to maintain normal microvessel permeability and a barrier between the circulating cells and endothelial cells forming the vessel wall,the ESG should have an organized structure at the molecular level.Due to the limitations of optical and electron microscopy,the ultra-structure and organization of ESG has not been revealed until recent development of a super high resolution fluorescence optical microscope,STORM(Stochastic Optical Reconstruction Microscopy).The diffraction of a single fluorescence molecule can be described as the point spread function(PSF).When the light of wavelengthλexcites the fluorophore(emitter),the intensity profile of the spot is defined as the PSF with the width^0.6λ/NA,NA is the numerical aperture of the objective.The diffraction-limited image resolution,for a high numerical aperture objective lens,is^200 nm in the lateral direction and^500 nm in the axial direction,for a conventional fluorescence microscope.The key idea of the single-molecule localization microscopy is to light the molecule,in turn,to achieve the nanometer-level accuracy of their position and reconstruction into a super-resolution image,such as STORM.STORM employs photo-switching mechanisms to stochastically activate individual molecules(photo-switchable or photoactivatable fluorophores)within the diffraction-limited region at different times.Then images with sub-diffraction limit resolution are reconstructed from the measured positions of individual fluorophores[3].To trade the super spatial resolution(accuracy),STORM sacrifices its temporal resolution(efficiency)by switching the state and sequentially exciting the emitters at a high density.Rust et al[3]employed organic dyes and fluorescent proteins as photo-switchable emitters to trade temporal resolution for a super spatial resolution(~20 nm lateral and^50 nm axial at present,can go down to a couple of nanometers if using smaller peptides or antibody fragments instead of currently used whole anti-bodies),which is an order of magnitude higher than conventional confocal microscopy.In the current study,we employed STORM to reveal the major ultra-structural components of the ESG,HS and HA,and their organization at the surface of the cultured EC monolayer[4].Materials and methods We used newly acquired Nikon-STORM system to observe the ESG on in vitro EC(bEnd3,mouse brain microvascular endothelial cells)monolayers.After confluency,the bEnd3 cells were immunolabeled with anti-HS,fol-lowed by an ATT0488 conjugated goat anti-mouse IgG,and with biotinylated HA binding protein,followed by an AF647 conjugated anti-biotin.The ESG was then imaged by the STORM with a 100x/1.49 oil immersed lens.Multiple Reporters of ATT0488 and AF647 with alternating illumination were used to acquire the 3D images of HS and HA.The field of 256×256(40×40μm2)of HS and HA at the surface of ECs was obtained based on totally 40,000 of EM-CCD captured images for each reporter at a capturing speed of 19 ms/frame.Results HA is a long molecule weaving into a network which covers the endothelial luminal surface.The diameter of the HA segments is 185.3±44.7 nm,155.5±57.2 nm,and 156.9±56.1 nm,respectively,at the top,middle and bottom regions of the cell luminal surface.In contrast,HS is a shorter molecule,perpendicular to the cell surface.HA and HS are partially overlapped with each other at the endothelial luminal surface.We quantified the length,diameter,orientation,and density of HS at the top,middle and bottom regions of the endothelial surface.The diameter of the observed HS is 191.0±46.0 nm,284.3±71.1 nm,and 184.2±59.6 nm,and the length of the HS is 621.0±75.7 nm,651.0±118.0 nm,and 575.2±105.6 nm,respectively,at the top,middle and bottom regions of the cell luminal surface.For the HS orientation,its angle with the cell surface is 92.9±1.9,88.7±8.2,and 96.2±10.9 degree,respectively,at the top,middle and bottom regions.The angle of 90 degree is perfectly perpendicular to the cell surface.For the HS distribution,the average density is0.398 elements/μm2,0.345 elements/μm2 and 0.665 elements/μm2,respectively,and the distance between the adjacent HS is 1 694.4±628.1 nm,1 844.8±758.5 nm,and 1 221.9±450.7 nm,respectively,at the top,middle and bottom regions.Conclusions Our results suggest that HS plays a major role in mechanosensing and HA plays a major role in the molecular sieve,due to their organization,ultra-structure and distribution.展开更多
Comparied to traditional optical processing methods,numerical controlled surfacing techniques have irreplaceable effects especially in spherical and aspherical optics. In this paper,fabricating methods,mechanisms and ...Comparied to traditional optical processing methods,numerical controlled surfacing techniques have irreplaceable effects especially in spherical and aspherical optics. In this paper,fabricating methods,mechanisms and processes are analyzed. Also,some processing difficulties and problems are discussed. The possible improvements and feasible methods are given.展开更多
Inductively coupled plasma optical emission spectrometric approach(1CP-OES) is used to determine the potassium content as principal component in pyrotechnic compositions used for fireworks and firecrackers. Element ...Inductively coupled plasma optical emission spectrometric approach(1CP-OES) is used to determine the potassium content as principal component in pyrotechnic compositions used for fireworks and firecrackers. Element of potassium is conunonly found in potassium nitrate and potassium perchlorate in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that potassium nitrate content in pyrotechnics is between 10% to 60% and the potassium perchlorate content is between 20% to 70%,which counted in the content of potassium element is between 4% to 23%. Concept of this method: considering the weight of the sample is 400rag,constant volume is 1L and the concentration of potassium is between 10 mg/L to 90 mg/L in sample solution, the determination scope of the method for the potassium content would be between 1% to 23%.Further experiments proved that the fitting correlation coefficient of potassium calibration curve is 0.9997 or higher, recovery is 89.15%-100.23%.The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.展开更多
Inductively coupled plasma optical emission spectrometric approach(ICP-OES)is used to determine the magnesium and aluminum content as principal components in pyrotechnic compositions used for fireworks and firecrack...Inductively coupled plasma optical emission spectrometric approach(ICP-OES)is used to determine the magnesium and aluminum content as principal components in pyrotechnic compositions used for fireworks and firecrackers. Elements of magnesium and aluminum are commonly found in aluminum powder or magnesium-aluminum alloy powder in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that the magnesium content in pyrotechnics is between 8% to 30% and the aluminum content is between 8% to 35%(roughly).Concept of this method: suppose the weight of the sample is 400rag,constant volume is IL and the concentlation of magnesium and aluminum is between 12mg/L to 160mg/L in sample solution, the determination scope of the method for magnesium and aluminum content would be between 3% to 40%.Further experiments proved that the fitting correlation coefficient of the magnesium calibration curve is 0.9999 or higher, recovery is 101.01% -101.96%.The fitting correlation coefficient of the aluminum calibration curve is 0.9999 or higher, recovery is 99.36%-103.07%. The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.展开更多
We study the nonlinear dynamics of two-component Bose-Einstein condensates in one-dimensional pe-riodic optical lattice potentials.The stationary state perturbation solutions of the coupled two-component nonlinearSchr...We study the nonlinear dynamics of two-component Bose-Einstein condensates in one-dimensional pe-riodic optical lattice potentials.The stationary state perturbation solutions of the coupled two-component nonlinearSchr?dinger/Gross-Pitaevskii equations are constructed by using the direct perturbation method.Theoretical analysisrevels that the perturbation solution is the chaotic one,which indicates the existence of chaos and chaotic region inparameter space.The corresponding numerical calculation results agree well with the analytical results.By applying thechaotic perturbation solution,we demonstrate the atomic spatial population and the energy distribution of the systemare chaotic generally.展开更多
Arc erosion morphologies of Ag/MeO(10) electrical contact materials after 50000 operations under direct current of 19 V and 20 A and resistive load conditions were investigated using scanning electron microscope(SE...Arc erosion morphologies of Ag/MeO(10) electrical contact materials after 50000 operations under direct current of 19 V and 20 A and resistive load conditions were investigated using scanning electron microscope(SEM) and a 3D optical profiler(3DOP). The results indicated that 3DOP could supply clearer and more detailed arc erosion morphology information. Arc erosion resistance of Ag/SnO_2(10) electrical contact material was the best and that of Ag/CuO(10) was the worst. Arc erosion morphology of Ag/MeO(10) electrical contact materials mainly included three different types. Arc erosion morphologies of Ag/ZnO(10) and Ag/SnO_2(10) electrical contact materials were mainly liquid splash and evaporation, and those of Ag/CuO(10) and Ag/CdO(10) were mainly material transfer from anode to cathode. Arc erosion morphology of Ag/SnO_2(6)In_2O_3(4) electrical contact materials included both liquid splash, evaporation and material transfer. In addition, the formation process and mechanism on arc erosion morphology of Ag/MeO(10) electrical contact materials were discussed.展开更多
A closed form solution to the problem of segmenting multiple 3D motion models was proposed from straight-line optical flow. It introduced the multibody line optical flow constraint (MLOFC), a polynomial equation relat...A closed form solution to the problem of segmenting multiple 3D motion models was proposed from straight-line optical flow. It introduced the multibody line optical flow constraint (MLOFC), a polynomial equation relating motion models and line parameters. The motion models can be obtained analytically as the derivative of the MLOFC at the corresponding line measurement, without knowing the motion model associated with that line. Experiments on real and synthetic sequences were also presented.展开更多
Spatial modedivision multiplexing is emerging as a potential solution to further increasing optical fiber capacity and spectral efficiency. We report a dualmode, dualpolarization transmission method based on modeselec...Spatial modedivision multiplexing is emerging as a potential solution to further increasing optical fiber capacity and spectral efficiency. We report a dualmode, dualpolarization transmission method based on modeselective excitation and detection over a twomode fiber. In particular, we present 107 Gbit/s coherent optical OFDM (COOFDM) transmission over a 4.5 km twomode fiber using LP and LP. modes in which mode separation is performed optically.展开更多
The impurity-induced localization of two-component Bose-Einstein condensates loaded into deep one-dimensional optical lattices is studied both analytically and numerically. It is shown that, the analytical criteria fo...The impurity-induced localization of two-component Bose-Einstein condensates loaded into deep one-dimensional optical lattices is studied both analytically and numerically. It is shown that, the analytical criteria for self-trapping and moving soliton/breather of the primary-component condensate are modified significantly by an admixture of an impurity component (the second component). The realization of the self-trapped state and the moving soliton/breather states of the primary-component becomes more easy with the minor admixture of the impurity-component, even if the two components are partly overlapped.展开更多
A novel time-domain ultra-fast pulse shaping approach for multi-TBaud serial optical communication signal (e.g. QPSK and 16-QAM) generation based on the first-order Born approximation in feasible all-fiber long-period...A novel time-domain ultra-fast pulse shaping approach for multi-TBaud serial optical communication signal (e.g. QPSK and 16-QAM) generation based on the first-order Born approximation in feasible all-fiber long-period gratings is proposed and numerically demonstrated.展开更多
A distributed feedback diode laser (DFB-DL) based hygrometer combined with a long-path-length Herriot gas cell and waterless optical components was proposed and investigated. The main function of this sensor was to ...A distributed feedback diode laser (DFB-DL) based hygrometer combined with a long-path-length Herriot gas cell and waterless optical components was proposed and investigated. The main function of this sensor was to simultaneously improve the measurement reliability and resolution. A comparison test between a 10-cm normal transmission-type gas cell and a 3-m Herriot gas cell was carried out to demonstrate the improvement. Reliability improvement was achieved by influence suppression of water vapor inside optical components (WVOC) through combined action of the Herriot gas cell and waterless optical components. The influence of WVOC was suppressed from 726ppmv to 25ppmv using the Herriot gas cell. Moreover, combined with waterless optical components, the influence of WVOC was further suppressed to no more than 4ppmv. Resolution improvement from l l.7ppmv to 0.32ppmv was achieved mainly due to the application of the long-path-length Herriot gas cell. The results show that the proposed sensor has a good performance and considerable potential application in gas sensing, especially when probed gas possibly permeates into optical components.展开更多
Although marine and terrestrial emissions simultaneously affect the formation of atmospheric fine particles in coastal areas,knowledge on the optical properties and sources of water-soluble matter in these areas is st...Although marine and terrestrial emissions simultaneously affect the formation of atmospheric fine particles in coastal areas,knowledge on the optical properties and sources of water-soluble matter in these areas is still scarce.In this work,taking Qingdao,China as a typical coastal location,the chemical composition of PM_(2.5)duringwinter 2019was analyzed.Excitation-emission matrix fluorescence spectroscopy was combined with parallel factor analysis model to explain the components of water-soluble atmospheric chromophores of PM_(2.5).Our analysis indicated that NO_(3)^(-),NH_(4)^(+)and SO_(4)^(2-)ions accounted for 86.80%of the total ion mass,dominated by NO_(3)^(-).The ratio of[NO_(3)^(-)]/[SO_(4)^(2-)]was up to 2.42±0.84,suggesting thatmobile sources play an important role in local pollutants emission.The result of positive correlation between Abs_(365)with K^(+)suggests that biomass burning is an important source of water-soluble organic compounds(WSOC).Six types of fluorophores(C1-C6),all humic-like substances,were identified in WSOC.Humification index,biological index and fluorescence index in winter were 1.66±0.34,0.51±0.44 and 1.09±0.78,respectively,indicating that WSOC in Qingdao were mainly terrestrial organic matters.Overall,although the study area is close to the ocean,the contribution of terrestrial sources to PM_(2.5),especially vehicle exhaust and coal combustion,is still much higher than that of marine sources.Our study provides a more comprehensive understanding of chemical and optical properties of WSOC based on PM_(2.5)in coastal areas,and may provide ground for improving local air quality.展开更多
Probability of detection(POD)graphics allow for a change from qualitative to quantitative assessment for every damage detection system,and as such it is a main request for conventional non-destructive testing(NDT)tech...Probability of detection(POD)graphics allow for a change from qualitative to quantitative assessment for every damage detection system,and as such it is a main request for conventional non-destructive testing(NDT)techniques.Its availability can greatly help towards the industrialization of the corresponding Structural health monitoring(SHM)system.But having in mind that for SHM systems the sensors are at fixed positions,and the location of a potential damage would change its detectability.Consequently robust simulation tools are required to obtain the model assisted probability of detection(MAPOD)which is needed to validate the SHM system.This tool may also help for the optimization of the sensor distribution,and finally will allow a probabilistic risk management.INDEUS,simulation of ultrasonic waves SHM system,was a main milestone in this direction.This article deals with the simulation tools for a strain based SHM system,using fiber optic sensors(FOS).FOS are essentially strain/temperature sensors,either with multi-point or with distributed sensing.The simulation tool includes the finite element model(FEM)for the original and damaged structure,and algorithms to compare the strain data at the pre-established sensors locations,and from this comparison to extract information about damage occurrence and location.The study has been applied to the structure of an all-composite unmanned aircraft vehicle(UAV)now under construction,designed at Universidad Politecnica de Madrid for the inspection of electrical utilities networks.Distributed sensing optical fibers were internally bonded at the fuselage and wing.Routine inspection is planned to be done with the aircraft at the test bench by imposing known loads.From the acquired strain data,damage occurrence may be calculated as slight deviations from the baselines.This is a fast inspection procedure without requiring trained specialists,and it would allow for detection of hidden damages.Simulation indicates that stringer partial debondings are detected before they become critical,while small delaminations as those produced by barely visible impact damages would require a prohibited number of sensing lines.These simulation tools may easily be applied to any other complex structure,just by changing the FEM models.From these results it is shown how a fiber optic based SHM system may be used as a reliable damage detection procedure.展开更多
基金the Joint Funds of National Natural Science Foundation of China and China Academy of Engineering Physics(NSAF)(No.U2030109)National Natural Science Foundation of China(No.52075129)。
文摘Low-pressure air plasma cleaning is an effective method for removing organic contaminants on large-aperture optical components in situ in the inertial confinement fusion facility.Chemical reactions play a significant role in plasma cleaning,which is a complex process involving abundant bond cleavage and species generation.In this work,experiments and reactive molecular dynamics simulations were carried out to unravel the reaction mechanism between the benchmark organic contaminants of dibutyl phthalate and air plasma.The optical emission spectroscopy was used to study the overall evolution behaviors of excited molecular species and radical signals from air plasma as a reference to simulations.Detailed reaction pathways were revealed and characterized,and specific intermediate radicals and products were analyzed during experiments and simulation.The reactive species in the air plasma,such as O,HO_(2)and O_(3)radicals,played a crucial role in cleaving organic molecular structures.Together,our findings provide an atomic-level understanding of complex reaction processes of low-pressure air plasma cleaning mechanisms and are essential for its application in industrial plasma cleaning.
基金supported by the National Key Research and Development Program of China(No.2018YFA0702800)the National Natural Science Foundation of China(No.12072056)supported by National Defense Fundamental Scientific Research Project(XXXX2018204BXXX).
文摘The safety and integrity requirements of aerospace composite structures necessitate real-time health monitoring throughout their service life.To this end,distributed optical fiber sensors utilizing back Rayleigh scattering have been extensively deployed in structural health monitoring due to their advantages,such as lightweight and ease of embedding.However,identifying the precise location of damage from the optical fiber signals remains a critical challenge.In this paper,a novel approach which namely Modified Sliding Window Principal Component Analysis(MSWPCA)was proposed to facilitate automatic damage identification and localization via distributed optical fiber sensors.The proposed method is able to extract signal characteristics interfered by measurement noise to improve the accuracy of damage detection.Specifically,we applied the MSWPCA method to monitor and analyze the debonding propagation process in honeycomb sandwich panel structures.Our findings demonstrate that the training model exhibits high precision in detecting the location and size of honeycomb debonding,thereby facilitating reliable and efficient online assessment of the structural health state.
文摘Purpose: To establish the prevalence and severity of myopia among the Chinese Hong Kong students and to study the relationship between myopia and optical components.Methods;One thousand and seventy-five freshmen of the 1993-1994 academic year in the Chinese University of Hong Kong underwent the eye examination including evaluation of refractive error, keratometry, and A-scan ultrasonic biometry. The data were analyzed with the SPSS/PC+4. 01 statistical package. Results: The prevalence of myopia was 91. 7% with the mean refraction being -4. 00 ± 2. 64D in this young adult population. The statistical analyses demonstrated a significant correlation between refractive value and axial length of the globe (r=-0. 78), vitreous length (r=-0. 76), anterior chamber depth (r=-0. 33), lens thickness (r = 0. 13) and corneal curvature (r = 0. 19). Conclusion: The refractive status is mainly dependent on the axial length. In general, the higher the myopia was, the longer the eyeball, the deeper the anterior chamber,
基金supported by NIH-1SC1CA153325-01,NSF-MRI CBET 1337746 and 1UG3TR002151-01
文摘Introduction The endothelial cells(ECs)lining every blood vessel wall constantly expose to the mechanical forces generated by the blood flow.The EC responses to these hemodynamic forces play a critical role in the homeostasis of the circulatory system.In addition to forming a transport barrier between the blood and vessel wall,vascular ECs play important roles in regulating circulation functions.Besides biochemical stimuli,blood flow induced(hemodynamic)mechanical stimuli,such as shear stress,pressure and circumferential stretch,modulate EC morphology and functions by activating mechanosensors,signaling pathways,and gene and protein expressions.The EC responses to the hemodynamic forces(mechano-sensing and transduction)are critical to maintaining normal vascular functions.Failure in the mechano-sensing and transduction leads to serious vascular diseases including hypertension,atherosclerosis,aneurysms and thrombosis,to name a few[1].On the luminal surface of our blood vessels,there is a thin layer called endothelial surface glycocalyx(ESG)which consists of proteoglycans,glycosaminoglycans(GAGs)and glycoproteins.The GAGs in the ESG are heparan sulfate(HS),hyaluronic acid(HA),chondroitin sulfate(CS),and sialic acid(SA)[2].In order to play important roles in vascular functions,such as being a mechanosensor and transducer for the endothelial cells(ECs)to sense the blood flow,a molecular sieve to maintain normal microvessel permeability and a barrier between the circulating cells and endothelial cells forming the vessel wall,the ESG should have an organized structure at the molecular level.Due to the limitations of optical and electron microscopy,the ultra-structure and organization of ESG has not been revealed until recent development of a super high resolution fluorescence optical microscope,STORM(Stochastic Optical Reconstruction Microscopy).The diffraction of a single fluorescence molecule can be described as the point spread function(PSF).When the light of wavelengthλexcites the fluorophore(emitter),the intensity profile of the spot is defined as the PSF with the width^0.6λ/NA,NA is the numerical aperture of the objective.The diffraction-limited image resolution,for a high numerical aperture objective lens,is^200 nm in the lateral direction and^500 nm in the axial direction,for a conventional fluorescence microscope.The key idea of the single-molecule localization microscopy is to light the molecule,in turn,to achieve the nanometer-level accuracy of their position and reconstruction into a super-resolution image,such as STORM.STORM employs photo-switching mechanisms to stochastically activate individual molecules(photo-switchable or photoactivatable fluorophores)within the diffraction-limited region at different times.Then images with sub-diffraction limit resolution are reconstructed from the measured positions of individual fluorophores[3].To trade the super spatial resolution(accuracy),STORM sacrifices its temporal resolution(efficiency)by switching the state and sequentially exciting the emitters at a high density.Rust et al[3]employed organic dyes and fluorescent proteins as photo-switchable emitters to trade temporal resolution for a super spatial resolution(~20 nm lateral and^50 nm axial at present,can go down to a couple of nanometers if using smaller peptides or antibody fragments instead of currently used whole anti-bodies),which is an order of magnitude higher than conventional confocal microscopy.In the current study,we employed STORM to reveal the major ultra-structural components of the ESG,HS and HA,and their organization at the surface of the cultured EC monolayer[4].Materials and methods We used newly acquired Nikon-STORM system to observe the ESG on in vitro EC(bEnd3,mouse brain microvascular endothelial cells)monolayers.After confluency,the bEnd3 cells were immunolabeled with anti-HS,fol-lowed by an ATT0488 conjugated goat anti-mouse IgG,and with biotinylated HA binding protein,followed by an AF647 conjugated anti-biotin.The ESG was then imaged by the STORM with a 100x/1.49 oil immersed lens.Multiple Reporters of ATT0488 and AF647 with alternating illumination were used to acquire the 3D images of HS and HA.The field of 256×256(40×40μm2)of HS and HA at the surface of ECs was obtained based on totally 40,000 of EM-CCD captured images for each reporter at a capturing speed of 19 ms/frame.Results HA is a long molecule weaving into a network which covers the endothelial luminal surface.The diameter of the HA segments is 185.3±44.7 nm,155.5±57.2 nm,and 156.9±56.1 nm,respectively,at the top,middle and bottom regions of the cell luminal surface.In contrast,HS is a shorter molecule,perpendicular to the cell surface.HA and HS are partially overlapped with each other at the endothelial luminal surface.We quantified the length,diameter,orientation,and density of HS at the top,middle and bottom regions of the endothelial surface.The diameter of the observed HS is 191.0±46.0 nm,284.3±71.1 nm,and 184.2±59.6 nm,and the length of the HS is 621.0±75.7 nm,651.0±118.0 nm,and 575.2±105.6 nm,respectively,at the top,middle and bottom regions of the cell luminal surface.For the HS orientation,its angle with the cell surface is 92.9±1.9,88.7±8.2,and 96.2±10.9 degree,respectively,at the top,middle and bottom regions.The angle of 90 degree is perfectly perpendicular to the cell surface.For the HS distribution,the average density is0.398 elements/μm2,0.345 elements/μm2 and 0.665 elements/μm2,respectively,and the distance between the adjacent HS is 1 694.4±628.1 nm,1 844.8±758.5 nm,and 1 221.9±450.7 nm,respectively,at the top,middle and bottom regions.Conclusions Our results suggest that HS plays a major role in mechanosensing and HA plays a major role in the molecular sieve,due to their organization,ultra-structure and distribution.
基金the National Natural Science Foundation (59875008)Beijing Nova Program of China (2006B24)
文摘Comparied to traditional optical processing methods,numerical controlled surfacing techniques have irreplaceable effects especially in spherical and aspherical optics. In this paper,fabricating methods,mechanisms and processes are analyzed. Also,some processing difficulties and problems are discussed. The possible improvements and feasible methods are given.
文摘Inductively coupled plasma optical emission spectrometric approach(1CP-OES) is used to determine the potassium content as principal component in pyrotechnic compositions used for fireworks and firecrackers. Element of potassium is conunonly found in potassium nitrate and potassium perchlorate in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that potassium nitrate content in pyrotechnics is between 10% to 60% and the potassium perchlorate content is between 20% to 70%,which counted in the content of potassium element is between 4% to 23%. Concept of this method: considering the weight of the sample is 400rag,constant volume is 1L and the concentration of potassium is between 10 mg/L to 90 mg/L in sample solution, the determination scope of the method for the potassium content would be between 1% to 23%.Further experiments proved that the fitting correlation coefficient of potassium calibration curve is 0.9997 or higher, recovery is 89.15%-100.23%.The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.
文摘Inductively coupled plasma optical emission spectrometric approach(ICP-OES)is used to determine the magnesium and aluminum content as principal components in pyrotechnic compositions used for fireworks and firecrackers. Elements of magnesium and aluminum are commonly found in aluminum powder or magnesium-aluminum alloy powder in pyrotechnic compositions in fireworks and firecrackers. Statistical analysis shows that the magnesium content in pyrotechnics is between 8% to 30% and the aluminum content is between 8% to 35%(roughly).Concept of this method: suppose the weight of the sample is 400rag,constant volume is IL and the concentlation of magnesium and aluminum is between 12mg/L to 160mg/L in sample solution, the determination scope of the method for magnesium and aluminum content would be between 3% to 40%.Further experiments proved that the fitting correlation coefficient of the magnesium calibration curve is 0.9999 or higher, recovery is 101.01% -101.96%.The fitting correlation coefficient of the aluminum calibration curve is 0.9999 or higher, recovery is 99.36%-103.07%. The allowable differential value is 0.4% between two single tests under repeatable conditions. This method can completely satisfy the requirements of the fireworks and firecrackers industry with high accuracy and good precision.
基金National Natural Science Foundation of China under Grant No.10575034Natural Science Foundation of Hunan Province of China under Grant Nos.06JJ2014 and 04JJ40006
文摘We study the nonlinear dynamics of two-component Bose-Einstein condensates in one-dimensional pe-riodic optical lattice potentials.The stationary state perturbation solutions of the coupled two-component nonlinearSchr?dinger/Gross-Pitaevskii equations are constructed by using the direct perturbation method.Theoretical analysisrevels that the perturbation solution is the chaotic one,which indicates the existence of chaos and chaotic region inparameter space.The corresponding numerical calculation results agree well with the analytical results.By applying thechaotic perturbation solution,we demonstrate the atomic spatial population and the energy distribution of the systemare chaotic generally.
基金Project(2012QNZT003)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(2012M521542)supported by the Postdoctoral Science Foundation of China+1 种基金Project(14JJ3014)supported by the Hunan Provincial Natural Science Foundation of ChinaProject(BSh1202)supported by the Zhejiang Provincial Postdoctoral Scientific Research Foundation of China
文摘Arc erosion morphologies of Ag/MeO(10) electrical contact materials after 50000 operations under direct current of 19 V and 20 A and resistive load conditions were investigated using scanning electron microscope(SEM) and a 3D optical profiler(3DOP). The results indicated that 3DOP could supply clearer and more detailed arc erosion morphology information. Arc erosion resistance of Ag/SnO_2(10) electrical contact material was the best and that of Ag/CuO(10) was the worst. Arc erosion morphology of Ag/MeO(10) electrical contact materials mainly included three different types. Arc erosion morphologies of Ag/ZnO(10) and Ag/SnO_2(10) electrical contact materials were mainly liquid splash and evaporation, and those of Ag/CuO(10) and Ag/CdO(10) were mainly material transfer from anode to cathode. Arc erosion morphology of Ag/SnO_2(6)In_2O_3(4) electrical contact materials included both liquid splash, evaporation and material transfer. In addition, the formation process and mechanism on arc erosion morphology of Ag/MeO(10) electrical contact materials were discussed.
基金The National Natural Science Foundation of China (No. 60675017) The National Basic Research Program (973) of China (No. 2006CB303103)
文摘A closed form solution to the problem of segmenting multiple 3D motion models was proposed from straight-line optical flow. It introduced the multibody line optical flow constraint (MLOFC), a polynomial equation relating motion models and line parameters. The motion models can be obtained analytically as the derivative of the MLOFC at the corresponding line measurement, without knowing the motion model associated with that line. Experiments on real and synthetic sequences were also presented.
文摘Spatial modedivision multiplexing is emerging as a potential solution to further increasing optical fiber capacity and spectral efficiency. We report a dualmode, dualpolarization transmission method based on modeselective excitation and detection over a twomode fiber. In particular, we present 107 Gbit/s coherent optical OFDM (COOFDM) transmission over a 4.5 km twomode fiber using LP and LP. modes in which mode separation is performed optically.
基金Project supported by the National Natural Science Foundation of China (Grant Nos.10774120 and 10975114)the Natural Science Foundation of Gansu Province of China (Grant No.1010RJZA012)the Natural Science Foundation of Northwest Normal University of China (Grant No.NWNU-KJCXGC-03-48)
文摘The impurity-induced localization of two-component Bose-Einstein condensates loaded into deep one-dimensional optical lattices is studied both analytically and numerically. It is shown that, the analytical criteria for self-trapping and moving soliton/breather of the primary-component condensate are modified significantly by an admixture of an impurity component (the second component). The realization of the self-trapped state and the moving soliton/breather states of the primary-component becomes more easy with the minor admixture of the impurity-component, even if the two components are partly overlapped.
基金supported in part by the Natural Sciences and En-gineering Research Council of Canada(NSERC),and le Fonds Qué-bécois de la Recherche sur la Nature et les Technologies(FQRNT).
文摘A novel time-domain ultra-fast pulse shaping approach for multi-TBaud serial optical communication signal (e.g. QPSK and 16-QAM) generation based on the first-order Born approximation in feasible all-fiber long-period gratings is proposed and numerically demonstrated.
基金This work was supported by the National Natural Science Foundation of China (60977058 & 61475085), the Science and Technology Development Project of Shandong Province (2014GGX101007), and the Fundamental Research Funds of Shandong University (2014YQ011).
文摘A distributed feedback diode laser (DFB-DL) based hygrometer combined with a long-path-length Herriot gas cell and waterless optical components was proposed and investigated. The main function of this sensor was to simultaneously improve the measurement reliability and resolution. A comparison test between a 10-cm normal transmission-type gas cell and a 3-m Herriot gas cell was carried out to demonstrate the improvement. Reliability improvement was achieved by influence suppression of water vapor inside optical components (WVOC) through combined action of the Herriot gas cell and waterless optical components. The influence of WVOC was suppressed from 726ppmv to 25ppmv using the Herriot gas cell. Moreover, combined with waterless optical components, the influence of WVOC was further suppressed to no more than 4ppmv. Resolution improvement from l l.7ppmv to 0.32ppmv was achieved mainly due to the application of the long-path-length Herriot gas cell. The results show that the proposed sensor has a good performance and considerable potential application in gas sensing, especially when probed gas possibly permeates into optical components.
基金supported by the National Natural Science Foundation of China(Nos.22076099,21876098,41877354)Youth Innovation Program of Universities in Shandong Province(No.2019KJD007)Fundamental Research Fund of Shandong University(No.2020QNQT012)
文摘Although marine and terrestrial emissions simultaneously affect the formation of atmospheric fine particles in coastal areas,knowledge on the optical properties and sources of water-soluble matter in these areas is still scarce.In this work,taking Qingdao,China as a typical coastal location,the chemical composition of PM_(2.5)duringwinter 2019was analyzed.Excitation-emission matrix fluorescence spectroscopy was combined with parallel factor analysis model to explain the components of water-soluble atmospheric chromophores of PM_(2.5).Our analysis indicated that NO_(3)^(-),NH_(4)^(+)and SO_(4)^(2-)ions accounted for 86.80%of the total ion mass,dominated by NO_(3)^(-).The ratio of[NO_(3)^(-)]/[SO_(4)^(2-)]was up to 2.42±0.84,suggesting thatmobile sources play an important role in local pollutants emission.The result of positive correlation between Abs_(365)with K^(+)suggests that biomass burning is an important source of water-soluble organic compounds(WSOC).Six types of fluorophores(C1-C6),all humic-like substances,were identified in WSOC.Humification index,biological index and fluorescence index in winter were 1.66±0.34,0.51±0.44 and 1.09±0.78,respectively,indicating that WSOC in Qingdao were mainly terrestrial organic matters.Overall,although the study area is close to the ocean,the contribution of terrestrial sources to PM_(2.5),especially vehicle exhaust and coal combustion,is still much higher than that of marine sources.Our study provides a more comprehensive understanding of chemical and optical properties of WSOC based on PM_(2.5)in coastal areas,and may provide ground for improving local air quality.
基金supported by the project TRA2014-58263-C2-2-Rfunded by the National Research program of Spain
文摘Probability of detection(POD)graphics allow for a change from qualitative to quantitative assessment for every damage detection system,and as such it is a main request for conventional non-destructive testing(NDT)techniques.Its availability can greatly help towards the industrialization of the corresponding Structural health monitoring(SHM)system.But having in mind that for SHM systems the sensors are at fixed positions,and the location of a potential damage would change its detectability.Consequently robust simulation tools are required to obtain the model assisted probability of detection(MAPOD)which is needed to validate the SHM system.This tool may also help for the optimization of the sensor distribution,and finally will allow a probabilistic risk management.INDEUS,simulation of ultrasonic waves SHM system,was a main milestone in this direction.This article deals with the simulation tools for a strain based SHM system,using fiber optic sensors(FOS).FOS are essentially strain/temperature sensors,either with multi-point or with distributed sensing.The simulation tool includes the finite element model(FEM)for the original and damaged structure,and algorithms to compare the strain data at the pre-established sensors locations,and from this comparison to extract information about damage occurrence and location.The study has been applied to the structure of an all-composite unmanned aircraft vehicle(UAV)now under construction,designed at Universidad Politecnica de Madrid for the inspection of electrical utilities networks.Distributed sensing optical fibers were internally bonded at the fuselage and wing.Routine inspection is planned to be done with the aircraft at the test bench by imposing known loads.From the acquired strain data,damage occurrence may be calculated as slight deviations from the baselines.This is a fast inspection procedure without requiring trained specialists,and it would allow for detection of hidden damages.Simulation indicates that stringer partial debondings are detected before they become critical,while small delaminations as those produced by barely visible impact damages would require a prohibited number of sensing lines.These simulation tools may easily be applied to any other complex structure,just by changing the FEM models.From these results it is shown how a fiber optic based SHM system may be used as a reliable damage detection procedure.