Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater. We designed an indoor program of A. percula culture in brackishwater with a salinity of 24±1, during which ...Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater. We designed an indoor program of A. percula culture in brackishwater with a salinity of 24±1, during which the impacts of feed type, water temperature, and light intensity, on the efficiency of its reproduction, were revealed. The fish were accommodated along with sea anemones in fibre glass tanks to determine the influence of brooder diet on breeding efficiency. Higher reproductive efficiency [number of eggs laid （276±22.3 eggs）] was observed when fish were fed live Acetes sp. rather than clam （204±16.4 eggs）, trash fish （155±12 eggs） and formulated feed （110±10 eggs）. The spawning rate was increased during September and October （water temperature, 28.74±0.55℃） on average of 2.4 spawning per month; and low spawning rate was in January （water temperature, 24.55±0.45℃） on average of I spawning per month. Among three light intensities （100, 500, and 900 1x） set to evaluate larval survival rate, larvae showed the highest survival rate （65.5%） at 900 lx. The breeding method specifically in brackishwater developed in the present study is a new approach, will help the people from the regions of estuary and backwater to enhance their livelihood and it will lead to reduce the exploitation from the wild habitat.展开更多
Effects of varied types of live feeds and pelleted feeds on the bioenergetics and reproductive performances were studied under laboratory conditions.The highest feeding rate of 279.4 J/g live fish/day was observed in ...Effects of varied types of live feeds and pelleted feeds on the bioenergetics and reproductive performances were studied under laboratory conditions.The highest feeding rate of 279.4 J/g live fish/day was observed in test fishes feed with Moina.Fish fed with pelleted feed exhibited the lowest feeding rate of 104.4 J/g live fish/day.But the absorption efficiency showed the opposite trend.The fish fed with pelleted feed showed the highest absorption efficiency 36%whereas the fish fed with Brachionus plicatilis exhibited the lowest absorption efficiency of 28%.The brood stock maintenance and larval growth were closely observed.The larvae grew like adult after 25th day of hatching.The mortality noticed during 2nd and 7th day of hatching was overcome by feeding the larvae with nutrient rich live feed.The sexually mature females and males were injected with 50μg of GnRHa(gonadotrophin releasing hormone analogue)encapsulated in a biodegradable copolymer of fatty acid dimer and sebacic acid p(FAD-SA)of a 25:75 molar ratio.After fifteen days all treated females commenced spawning.Similar treatment induced a two-fold increase in sperm volume in male.Conclusion of the present study indicated that the laboratory scale production of marine clown fish,Amphiprion sebae can be successfully carried out using live feed than the pellet feeds which express better feeding rate,spawning and development.展开更多
文摘Breeding and mass scale larval rearing of clownfish Amphiprion percula is very limited in brackishwater. We designed an indoor program of A. percula culture in brackishwater with a salinity of 24±1, during which the impacts of feed type, water temperature, and light intensity, on the efficiency of its reproduction, were revealed. The fish were accommodated along with sea anemones in fibre glass tanks to determine the influence of brooder diet on breeding efficiency. Higher reproductive efficiency [number of eggs laid （276±22.3 eggs）] was observed when fish were fed live Acetes sp. rather than clam （204±16.4 eggs）, trash fish （155±12 eggs） and formulated feed （110±10 eggs）. The spawning rate was increased during September and October （water temperature, 28.74±0.55℃） on average of 2.4 spawning per month; and low spawning rate was in January （water temperature, 24.55±0.45℃） on average of I spawning per month. Among three light intensities （100, 500, and 900 1x） set to evaluate larval survival rate, larvae showed the highest survival rate （65.5%） at 900 lx. The breeding method specifically in brackishwater developed in the present study is a new approach, will help the people from the regions of estuary and backwater to enhance their livelihood and it will lead to reduce the exploitation from the wild habitat.
基金The authors are grateful to the secretary,principal,of Kamaraj College,Tuticorin,for providing their support to carry out this research workThe authors also would like to express sincere gratitude to the funding agency,OSTC(Ocean Science and Technology),New Delhi Project Sanctioned No:30.3.2000/Ref:DOT/11-MRVF/4/1/UN/97/P-5.
文摘Effects of varied types of live feeds and pelleted feeds on the bioenergetics and reproductive performances were studied under laboratory conditions.The highest feeding rate of 279.4 J/g live fish/day was observed in test fishes feed with Moina.Fish fed with pelleted feed exhibited the lowest feeding rate of 104.4 J/g live fish/day.But the absorption efficiency showed the opposite trend.The fish fed with pelleted feed showed the highest absorption efficiency 36%whereas the fish fed with Brachionus plicatilis exhibited the lowest absorption efficiency of 28%.The brood stock maintenance and larval growth were closely observed.The larvae grew like adult after 25th day of hatching.The mortality noticed during 2nd and 7th day of hatching was overcome by feeding the larvae with nutrient rich live feed.The sexually mature females and males were injected with 50μg of GnRHa(gonadotrophin releasing hormone analogue)encapsulated in a biodegradable copolymer of fatty acid dimer and sebacic acid p(FAD-SA)of a 25:75 molar ratio.After fifteen days all treated females commenced spawning.Similar treatment induced a two-fold increase in sperm volume in male.Conclusion of the present study indicated that the laboratory scale production of marine clown fish,Amphiprion sebae can be successfully carried out using live feed than the pellet feeds which express better feeding rate,spawning and development.
