Raman and UV-Vis Spectroscopy Applied to the Analysis of Liver Tissues from Rats with Myocardial Ischemia Induced by Isoproterenol

The application of the laser Raman spectroscopic（LRS） technique for the analysis of liver tissues from rats with myocardial ischemia induced by isoproterenol（ISO） was described.Animal model of myocardial ischemia was established for rats induced by ISO.Rats were randomly divided into four groups as normal group and myocardial ischemia groups.We observed the successful myocardial ischemia model via serum enzymes levels and hematoxylin-eosin（HE） staining,and detected the liver tissue of the rats from normal group and liver tissue of the rats from myocardial ischemia groups via UV-Vis spectroscopy（UV-Vis） and LRS,and the changes of the absorbance spectra were compared in the above four different groups.The results show that ISO can induce rat myocardial ischemia successfully.The spectrum of normal liver tissue supernatant exhibits a strong absorption band at 968 nm,but no absorption band appears in the spectra of liver tissue supernatant solutions from the rats with myocardial ischemia induction after 2,12 and 72 h presented at 968 nm.LRS results show that Raman intensities of the precipitates suffered from ISO-treatment after 2,12 and 72 h were obviously increased compared with that of the precipitate of the liver tissue of the normal rats suffered from 0.9 g/L normal saline（NS） treatment.These results indicate that LRS and UV-Vis can be harmless,nondestructive,rapid and effective methods for analyzing different pathological specimens of liver tissue from myocardial ischemia rats.

Structural deformation of nitro group of nitromethane molecule in liquid phase in an intense femtosecond laser field

The structural deformation of NO2 group induced by an intense femtosecond laser field of liquid nitromethane（NM）molecule is detected by time-and frequency-resolved coherent anti-Stokes Raman spectroscopy（CARS） technique with the intense pump laser. Here, we present the mechanism of molecular alignment and deformation. The CARS spectra and its FFT spectra of liquid NM show that the NO2 torsional mode couples with the CN symmetric stretching mode and that the NO2 group undergoes ultrafast structural deformation with a relaxation time of 195 fs. The frequency of the NO2 torsional mode in liquid NM（50.8±0.3 cm^-1） at room temperature is found. Our results prove the structural deformation of two groups in liquid NM molecule occur simultaneously in the intense laser field.

Single-cell analysis reveals microbial spore responses to microwave radiation

To determine the effects of microwave radiation at the molecular level as well as on the germination,growth and morphology of dry spores at the single-cell level.Dry Bacillus aryabhattai MCCC 1K02966 spores were microwave-treated at different powers and characterized using single-cell optical technology.As determined by laser tweezers Raman spectroscopy,the Ca^(2+)-dipicolinic acid content increased and nucleic acid denaturation occurred in response to microwave treatment.Livecell microscopy revealed that the germination and growth rates decreased as the microwave power increased.With respect to morphology,atomic force microscopy(AFM)demonstrated that spores became wrinkled and rough after microwave treatment.Furthermore,spores became smaller as the microwave power increased.Microwave treatment can damage DNA,and high-power microwaves can inhibit the germination of spores and reduce spore volumes.These results provide a new perspective on the responses of living single cells to microwave radiation and demonstrate the application of various new techniques for analyses of microorganisms at the single-cell level.

Fluid Inclusions and Metallization of the Kendekeke Polymetallic Deposit in Qinghai Province, China

The Kendekeke polymetallic deposit,located in the middle part of the magmatic arc belt of Qimantag on the southwestern margin of the Qaidam Basin,is a polygenetic compound deposit in the Qimantag metallogenic belt of Qinghai Province.Multi-periodic ore-forming processes occurred in this deposit,including early-stage iron mineralization and lead-zinc-gold-polymetallic mineralization which was controlled by later hydrothermal process.The characteristics of the ore-forming fluids and mineralization were discussed by using the fluid inclusion petrography,Laser Raman Spectrum and micro-thermometry methods.Three stages,namely,S1-stage （copper-iron-sulfide stage）,S2-stage （lead-zinc-sulfide stage） and C-stage （carbonate stage） were included in the hydrothermal process as indicated by the results of this study.The fluid inclusions are in three types：aqueous inclusion （type I）,CO2-aqueous inclusion （type Ⅱ） and pure CO2 inclusion （type Ⅲ）.Type Ⅰ inclusions were observed in the S1-stage,having homogenization temperature at 240-320℃,and salinities ranging from 19.8％ to 25.0％ （wt％ NaCl equiv.）.All three types of inclusions,existing as immiscible inclusion assemblages,were presented in the S2-stage,with the lowest homogenization temperature ranging from 175 ℃ to 295℃,which represents the metallogenic temperature of the S2-stage.The salinities of these inclusions are in the range of 1.5％ to 16％.The fluid inclusions in the C-stage belong to types Ⅰ,Ⅱ and Ⅲ,having homogenization temperatures at 120-210℃,and salinities ranging from 0.9％ to 14.5％.These observations indicate that the ore-forming fluids evolved from high-temperature to low-temperature,from high-salinity to low-salinity,from homogenization to immiscible separation.Results of Laser Raman Spectroscopy show that high density of CO2 and CH4 were found as gas compositions in the inclusions.CO2,worked as the pH buffer of ore-forming fluids,together with reduction of organic gases （i.e.CH4,etc）,affected the transport and sediment of the minerals.The fluid system alternated between open and close systems,namely,between lithostatic pressure and hydrostatic pressure systems.The calculated metallogenic pressures are in the range of 30 to 87 Mpa corresponding to 3 km mineralization depth.Under the influence of tectonic movements,immiscible separation occurred in the original ore-forming fluids,which were derived from the previous high-salinity,high-temperature magmatic fluids.The separation of CO2 changed the physicochemical properties and composition of the original fluids,and then diluted by mixing with extraneous fluids such as meteoric water and groundwater,and metallogenic materials in the fluids such as lead,zinc and gold were precipitated.

Ore-forming Fluid Characteristics of the Saishitang Cu-polymetallic Deposit in Qinghai Province,China

Saishitang Cu-polymetallic deposit is located in the southeast section of Late Paleozoic arcfoid in the southeastern margin of Qaidam platform. Accoring to the geological process of the deposit, four mineralization episodes were identified： melt/fluid coexisting period （O）, skarn period （A）, first sulfide period （B） and second sulfide period （C）, and 10 stages were finally subdivided. Three types of inclusions were classified in seven stages, namely crystal bearing inclusions （type I）, aqueous inclusions （type Ⅱ） and pure liquid inclusions （type Ⅲ）. Type I and Ⅱ inclusions were observed in stage O1, having homogenization temperature from 252 to 431℃, and salinities ranging from 24.3% to 48.0%. Type I inclusion was present in stage A1, having homogenization temperature from 506 to 548℃, and salinities ranging from 39.4% to 44.6%. In stage B1, type Ⅱ and Ⅲ inclusions were observed, with homogenization temperature concentrating between 300-400℃, and salinities from 0.4% to 4.3%. Type II inclusions were present in stage B2, with homogenization temperature varying from 403 to 550℃. In stage C1, type I and II inclusion commonly coexisted, and constituted a boiling inclusion group, having homogenization temperatures at 187-463℃, and salinities in a range of 29.4%-46.8% and 2.2%-11.0%. Type II and III inclusions were developed in stage C2, having homogenization temperature at 124-350℃, and salinities ranging between 1.6% and 15.4%. In stage C3, type Ⅱ and Ⅲ inclusions were presented, with a homogenization temperature range of 164-360℃, and salinities varying from 4.0% to 11.0%. The results of micro-thermal analysis show that fluids are characterized by high temperature and high salinity in stage O1 and A1, and experienced slight decrease in temperature and dramatic decrease in salinity in stage B1 and B2. In stage C1, the salinity of fluid increased greatly and a further decrease of temperature and salinity occurred in stage C2 and C3. Fluids boiled in stage C1. With calculated pressure of 22 MPa from the trapping temperature of 284- 289℃, a mineralization depth of 2.2 km was inferred. Results of Laser Raman Spectroscopy show high density of H2_O, CH_4 and CO_2 were found as gas composition. H-O isotope study indicates the ore- forming fluids were the mixture of magmatic water and meteoric water. Physicochemical parameters of fluids show oxygen and sulfur fugacity experienced a decrease, and redox state is weakly reducing. Along with fluid evolution, oxidation has increased slightly. Comprehensive analysis shows that melt exsolution occurred during the formation of quartz diorite and that metal elements existed and migrated in the form of chlorine complex. Immiscible fluid separation and boiling widely occurred after addition of new fluids, bringing about dissociation of chlorine-complex, resulting in a great deal of copper precipitation. In conclusion, Saishitang deposit, controlled by regional tectonics, is formed by metasomatism between highly fractionated mineralization rock body and wall rock, and belongs to banded skarn Cu-polymetallic deposit.Abstract： Saishitang Cu-polymetallic deposit is located in the southeast section of Late Paleozoic arcfoid in the southeastern margin of Qaidam platform. Accoring to the geological process of the deposit, four mineralization episodes were identified： melt/fluid coexisting period （O）, skarn period （A）, first sulfide period （B） and second sulfide period （C）, and 10 stages were finally subdivided. Three types of inclusions were classified in seven stages, namely crystal bearing inclusions （type I）, aqueous inclusions （type Ⅱ） and pure liquid inclusions （type Ⅲ）. Type I and II inclusions were observed in stage O1, having homogenization temperature from 252 to 431℃, and salinities ranging from 24.3% to 48.0%. Type I inclusion was present in stage A1, having homogenization temperature from 506 to 548℃, and salinities ranging from 39.4% to 44.6%. In stage B1, type II and III inclusions were observed, with homogenization temperature concentrating between 300-400℃, and salinities from 0.4% to 4.3%. Type II inclusions were present in stage B2, with homogenization temperature varying from 403 to 550℃. In stage C1, type I and II inclusion commonly coexisted, and constituted a boiling inclusion group, having homogenization temperatures at 187-463℃, and salinities in a range of 29.4%-46.8% and 2.2%-11.0%. Type II and III inclusions were developed in stage C2, having homogenization temperature at 124-350℃, and salinities ranging between 1.6% and 15.4%. In stage C3, type II and Ⅲ inclusions were presented, with a homogenization temperature range of 164-360℃, and salinities varying from 4.0% to 11.0%. The results of micro-thermal analysis show that fluids are characterized by high temperature and high salinity in stage O1 and A1, and experienced slight decrease in temperature and dramatic decrease in salinity in stage B1 and B2. In stage C1, the salinity of fluid increased greatly and a further decrease of temperature and salinity occurred in stage C2 and C3. Fluids boiled in stage C1. With calculated pressure of 22 MPa from the trapping temperature of 284- 289℃, a mineralization depth of 2.2 km was inferred. Results of Laser Raman Spectroscopy show high density of H_2O, CH_4 and CO_2 were found as gas composition. H-O isotope study indicates the ore- forming fluids were the mixture of magmatic water and meteoric water. Physicochemical parameters of fluids show oxygen and sulfur fugacity experienced a decrease, and redox state is weakly reducing. Along with fluid evolution, oxidation has increased slightly. Comprehensive analysis shows that melt exsolution occurred during the formation of quartz diorite and that metal elements existed and migrated in the form of chlorine complex. Immiscible fluid separation and boiling widely occurred after addition of new fluids, bringing about dissociation of chlorine-complex, resulting in a great deal of copper precipitation. In conclusion, Saishitang deposit, controlled by regional tectonics, is formed by metasomatism between highly fractionated mineralization rock body and wall rock, and belongs to banded skarn Cu-polymetallic deposit.