Critical dispersion of chirped fiber Bragg grating for eliminating time delay signature of distributed feedback laser chaos

Optical chaos has attracted widespread attention owing to its complex dynamic behaviors.However,the time delay signature(TDS)caused by the external cavity mode reduces the complexity of optical chaos.We propose and numerically demonstrate the critical dispersion of chirped fiber Bragg grating(CFBG)for eliminating the TDS of laser chaos in this work.The critical dispersion,as a function of relaxation frequency and bandwidth of the optical spectrum,is found through extensive dynamics simulations.It is shown that the TDS can be eliminated when the dispersion of CFBG is above this critical dispersion.In addition,the influence of dispersive feedback light and output light from a laser is investigated.These results provide important quantitative guidance for designing chaotic semiconductor lasers without TDS.

Influence of laser linewidth on performance of Brillouin optical time domain reflectometry

The effects of optical sources with different laser linewidths on Brillouin optical time domain reflectometry （BOTDR） are investigated numerically and experimentally. Simulation results show that the spectral linewidth of spontaneous Brillouin scattering remains almost constant when the laser linewidth is less than 1 MHz at the same pulse width; otherwise, it increases sharply. A comparison between a fiber laser （FL） with 4-kHz linewidth at 3 dB and a distributed feedback （DFB） laser with 3-MHz linewidth is made experimentally. When a constant laser power is launched into the sensing fiber, the fitting linewidths of the beat signals （backscattered Brillouin light and local oscillator （LO）） is about 5 MHz wider for the DFB laser than for the FL and the intensity of the beat signal is about a half. Furthermore, the frequency fluctuation in the long sensing fiber is lower for the FL source, yielding about 2 MHz less than that of the DFB laser, indicating higher temperature/strain resolution. The experimental results are in good agreement with the numerical simulations.

Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system

A laser scanning confocal imaging-surface plasmon resonance （LSCI-SPR） instrument integrated with a wavelength-dependent surface plasmon resonance （SPR） sensor and a laser scanning confocal microscopy （LSCM） is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.

Analysis of the time domain characteristics of tapered semiconductor lasers

We use traveling wave coupling theory to investigate the time domain characteristics of tapered semiconductor lasers with DBR gratings.We analyze the influence of the length of second order gratings on the power and spectrum of output light,and optimizing the length of gratings,in order to reduce the mode competition effect in the device,and obtain the high power output light wave with good longitudinal mode characteristics.

Formation and Growth of Silver Nanocubes upon Nanosecond Pulsed Laser Irradiation: Effects of Laser Intensity and Irradiation Time

Silver nanoparticles (AgNPs) were fabricated by repetitive irradiation of near ultraviolet (UV) nanosecond laser pulses (355 nm, 5 ns) in an aqueous solution of silver nitrate in the absence of stabilizing agents. A broad absorption peak was observed in the visible region showing the formation of a variety of AgNPs in the solution. Among the variety of products, it was found that silver nanocubes (AgNCs) grew in size with longer laser irradiation time. The size of AgNCs also increased with higher laser intensity. The average size of AgNCs, investigated by a scanning electron microscope (SEM) was in the range of 75 - 200 nm. The number of reduced atoms in AgNCs as a function of laser intensity showed that the AgNCs are apparently produced by a four photon process, implying that the formation of dimer silver atoms is essential for the formation.

Ignition time of self-propagating high-temperature synthesis by laser

The ignition of self propagating high temperature synthesis (SHS) by a laser beam has very well application, but there is lack in study on the ignition process. In order to search the rule of ignition process with laser beam, ignition time of SHS was studied in detail. First one dimension ignition model was introduced: burning is the process in which one layer is ignited by next layer. Then according to Fourier conduction equation, an equation used to calculate the ignition time was deduced. Finally a series of tests were made to verify the equation. The results prove that the change of the parameters in test agrees well with the equation.

Time-resolved shadowgraphs and morphology analyses of aluminum ablation with multiple femtosecond laser pulses

Aluminum ablation by multiple femtosecond laser pulses is investigated via time-resolved shadowgraphs and scanning electron microscope （SEM） images of the ablation spot. The spatial distribution of the ejected material and the radius of the shock wave generated during the ablation are found to vary with the increase in the number of pulses. In the initial two pulses, nearly concentric and semicircular stripes within the shock wave front are observed, unlike in subsequent pulses. Ablation by multiple femtosecond pulses exhibits different characteristics compared with the case induced by single femtosecond pulse because of the changes to the aluminum target surface induced by the preceding pulses.

The Study of Relaxation Time in Test of 940 nm Semiconductor Laser

Conventional test of the peak wavelength of a laser used to be applied immediately after a device is injected current. However, the results can not be considered as an accurate description to temperature characteristic. This passage puts forward a concept of relaxation time in wavelength texts, mainly based on the experiment of 940 nm strain quantum well laser, confirming that under constant current, wavelength will get through a process of rising, and then, reach the limit. This process brings the effect on spectral characteristics of a device which cannot be ignored and the accumulated heat in relaxation time will gradually impact the emission wavelength of the laser, even crest split to form bimodal phenomenon.

Time-Delayed Feedback Control in a Single-Mode Laser System

The effects of time-delayed feedback control in a single-mode laser system is investigated.Using the smalltime delay approximation,the analytic expression of the stationary probability distribution function of the laser field isobtained.The mean,normalized variance and skewness of the steady-state laser intensity are calculated.It is found thatthe time-delayed feedback control can suppress the intensity fluctuation of the laser system.The numerical simulationsare in good agreement with the approximate analytic results.

The isolation and identification of apolipoprotein C-I in hormone-refractory prostate cancer using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

Androgens play a central role in prostate cancer pathogenesis, and hence most of the patients respond to androgen deprivation therapies. However, patients tend to relapse with aggressive prostate cancer, which has been termed as hormone refractory. To identify the proteins that mediate progression to the hormone-refractory state, we used protein-chip technology for mass profiling of patients＇ sera. This study included 16 patients with metastatic hormone-refractory prostate cancer who were initially treated with androgen deprivation therapy. Serum samples were collected from each patient at five time points： point A, pre-treatment; point B, at the nadir of the prostate- specific antigen （PSA） level; point C, PSA failure; point D, the early hormone-refractory phase; and point E, the late hormone-refractory phase. Using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, we performed protein mass profiling of the patients＇ sera and identified a 6 640-Da peak that increased with disease progression. Target proteins were partially purified, and by amino acid sequencing the peak was identified as a fragment of apolipoprotein C-I （ApoC-I）. Serum ApoC-I protein levels increased with disease progression. On immunohistochemical analysis, the ApoC-i protein was found localized to the cytoplasm of the hormone-refractory cancer cells. In this study, we showed an increase in serum ApoC-I protein levels in prostate cancer patients during their progression to the hormone-refractory state, which suggests that ApoC-I protein is related to progression of prostate cancer. However, as the exact role of ApoC-I in prostate cancer pathogenesis is unclear, further research is required.

POTENTIAL OF IN VIVO LATENT-TIME ESTIMATION BY LASER AND OPTICAL TECHNIQUES IN CLINICAL AND EXPERIMENTAL DERMATOLOGY

The lag(latency)time(LT)is known in dermatology clinic as an asymptomatic period till the development of skin eruptions.In the laboratory,the LT might determine the interval from"zero"point until the peak(s)of changes in measured laboratory parameter during the performed test.This paper discusses methodological and technical aspects of precise measurement of the LT in the living healthy and pathological skin by laser and optical technologies through clinical and experimental applications in dermatology.Based on a dynamics approach to measure,calculate and interpret the LT in blood and in interstitialfluid compartments of the skin tissue,this method has a potential to promote deeper understanding of the role of complex dynamic processes in the skin at a level of a molecule,and/or an organ in a whole organism.The method of the LT measurement in vivo also promotes new understanding of(patho)physiological,diagnostic and pharmacological aspects of certain dynamic skin lesions and dynamic complex processes that happen in the skin.Utilized laser and optical techniques showed high reliability and objectivity in collecting data from rapidly changed skin lesions and processes,demonstrating the LT measurement as a very easy-to-use calculation procedure with high informativity,which is extremely important for the clinical and laboratory environment.

Laser, Universe and Arrow of Time

We have presented a comparison between the universe and the Laser. In many ways, the physics of laser and the universe are analogous. The root of the analogy is the fact that both laser and early universe depend completely on the quantum nature. We have also presented a simple analogous example of the growth of a flower at successive stages of development and shown how the arrow of time may be represented in these cases.

Study on Echo Time-Delay Estimation of Pulsed Laser Fuze

Pulse laser range detector is to measure the distance by estimating the time delay between the emitting pulse and echo pulse.In this paper,a mathematical model for the target echo signal of laser fuze has been established;in accordance with this model,the formulas for echo time-delay estimation and for amplitude estimation based on least squares criterion have been deduced.It is argued and simulated that the resolution of echo time-delay estimation could be improved through multi-reference correlation approach.Experiments illustrate that the approach enables pulsed laser fuze to perform high-precision ranging under a low signal-to-noise ratio condition.

Detection of K in soil using time-resolved laser-induced breakdown spectroscopy based on convolutional neural networks

One of the technical bottlenecks of traditional laser-induced breakdown spectroscopy(LIBS) is the difficulty in quantitative detection caused by the matrix effect. To troubleshoot this problem,this paper investigated a combination of time-resolved LIBS and convolutional neural networks(CNNs) to improve K determination in soil. The time-resolved LIBS contained the information of both wavelength and time dimension. The spectra of wavelength dimension showed the characteristic emission lines of elements, and those of time dimension presented the plasma decay trend. The one-dimensional data of LIBS intensity from the emission line at 766.49 nm were extracted and correlated with the K concentration, showing a poor correlation of R_c^2?=?0.0967, which is caused by the matrix effect of heterogeneous soil. For the wavelength dimension, the two-dimensional data of traditional integrated LIBS were extracted and analyzed by an artificial neural network(ANN), showing R_v^2?=?0.6318 and the root mean square error of validation(RMSEV)?=?0.6234. For the time dimension, the two-dimensional data of time-decay LIBS were extracted and analyzed by ANN, showing R_v^2?=?0.7366 and RMSEV?=?0.7855.These higher determination coefficients reveal that both the non-K emission lines of wavelength dimension and the spectral decay of time dimension could assist in quantitative detection of K.However, due to limited calibration samples, the two-dimensional models presented over-fitting.The three-dimensional data of time-resolved LIBS were analyzed by CNNs, which extracted and integrated the information of both the wavelength and time dimension, showing the R_v^2?=?0.9968 and RMSEV?=?0.0785. CNN analysis of time-resolved LIBS is capable of improving the determination of K in soil.

Two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry for detection of protein expression profiles in the hippocampus following closed brain injury

Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time, expression amount, protein post-translational processing or modification. Therefore, it is necessary to quantitatively analyze the gene expression profile using proteomic techniques. In the present study, we established a rat model of closed brain injury using Marmarou＇s weight-drop device, and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. A total of 364 protein peaks were detected on weak cation exchange-2 protein chips, including 37 differential protein peaks. 345 protein peaks were detected on immobilized metal affinity capture arrays-Cu, including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury. These results indicate that brain injury can alter protein expression in the hippocampus, and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.

Analysis of time phase of characteristic radiation in plasma induced by laser ablating aluminum

With time and space resolved technique, we have recorded time resolved spectra of irradiation of the plasma induced by Nd: YAG laser ablating metal Aluminum in Ar, in which, laser pulse energy was set up to 145 mJ/pulse and the buffer pressure 100 kpa. The continuum radiation and special emission of Aluminum plasma were studied based on the records. According to time distribution of Al Ⅰ396.15 nm emission, we analyzed the time differences between characteristic and continuum radiation evolving. We tried to explain the time phases of characteristic radiation evolving with traditional theoretical model of atomic transition. As the result, we found that it was difficult to explain our experimental results with the model. In order to explain our experimental results, we need new model or to improve the traditional theoretical model of atomic transition.

Quantum explanation for time phase relations in radiation of the plasma induced by laser ablating aluminum

According to time distribution of Al Ⅰ396.15 nm emission in the plasma induced by laser ablating Aluminum, based on quantum mechanics, we have suggested a hypothesis of transient steady state of atom, which could give our experimental results overall and reasonable explanation in quantum. We suggested that there should be a certain atomic state between ground and excited state of Aluminum atom, so called transient steady state. The transient steady state was that aluminum atom had already absorbed a certain photon, but the valence electron had not transited to external orbit. That is to say, aluminum atom had not transited into excited state, but changed into a certain state called transient steady state between ground and excited state. Seen from the point of atomic energy level, the transient steady state is identical to the level of excited state. The transient steady state was one of the most important models storing energy. The hypothesis could roundly and reasonably explain our experimental results.

Quantitative analysis of RNA levels from single hepatocytes in vivo: combined use of real-time RT-PCR and laser microdissection

AIM: The manner in which a cell responds to and influences its environment is ultimately determined by the genes that are expressed. To better understand cellular functions, the isolation of single cells and subsequent quantification of the expressed genes is essential. METHODS: Normal liver tissue was obtained from operation, snap-frozen in liquid nitrogen and sectioned in crystat. Individual hepatocytes were microdissected. RNA was extracted, then reverse transcribed and amplified using real-time quantitative polymerase chain reaction (PCR). RESULTS: Single hepatocytes were dissected by laser beam and catapulted to the microcentrifuge cap which was put above the slide. In this way, cells were collected, RNA was extracted, reverse transcribed to cDNA and used for analysis of RNA expression by real-time quantitative PCR. The amplification results showed that quantitation of the RNA inside the cell was compatible with the number of cells. CONCLUSION: The expression of RNA in single cells can be quantitated successfully by using laser microdissection and real-time PCR. These techniques provide an opportunity to monitor in vivo gene expression levels in single hepatocytes.

Time-resolved spectroscopy for 5s^('4)D_(7/2) state transitions undergoing electron–ion recombination in femtosecond laser-produced copper plasma

In the femtosecond laser-produced Cu-plasma, the transient transition dynamics that the excited state 5s4D7/2 via electron-ion recombination transfers to 4p4F9/20 （465.11 nm, Λ1 line） and 4p4D7/20 （529.25 nm, Λ2 line） states are investigated by using the time-resolved spectroscopy. The occupation number and relevant lifetime of the excited state 5s4D7/2, the temporal evolutions of spectral intensities for Λ1 line and Λ2 line emissions are demonstrated to be in direct proportion to the employed laser intensity, which reveals the transient features of transition dynamics clearly differing from that resulted in the traditional collision excitation. Furthermore, some unique characteristics for Λ1 and Λ2 transitions stemming from electron-ion recombination are examined in detail.