High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable component of the insulin signaling pathway.Silencing of Ls...High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable component of the insulin signaling pathway.Silencing of LsAkt severely hinders reproduction in Lasioderma serricorne,a stored product insect pest.However,the post-transcriptional pathway of LsAkt in L.serricorne remains unknown.This study identified 2 binding sites of miR-9c-5p and novel-mir50 in the coding sequences of LsAkt.The expression profiles of 2 microRNAs(miRNAs)and LsAkt displayed an opposite pattern during the adult stages.Luciferase reporter assay showed that novel-mir50 and miR-9c-5p could downregulate the expression of LsAkt.Overexpression of miR-9c-5p and novel-mir50 by injection of mimics inhibited the expression of LsAkt and reduced oviposition,decreased egg hatchability,and blocked ovarian development.It also decreased the expression of genes involved in ovarian development(LsVg and LsVgR)and the nutritional signaling pathway(LsTOR,LsS6K,and Ls4EBP),and reduced the phosphorylation of Akt.Conversely,injection of miR-9c-5p and novel-mir50 inhibitors induced the expressions of LsAkt,LsVg,LsVgR,LsTOR,LsS6K,and Ls4EBP,enhanced Akt phosphorylation level,and accelerated ovarian development.Injection of bovine insulin downregulated the expression of miR-9c-5p and novel-mir50 and upregulated the LsAkt expression.It also rescued the reproductive development defects associated with miR-9c-5p/novel-mir50 overexpression,forming a positive regulatory loop of insulin signaling.These results indicate that miR-9c-5p/novel-mir50 regulates the female reproduction of L.serricorne by targeting Akt in response to insulin signaling.The data also demonstrate the effects of the insulin/miRNA/Akt regulatory axis in insect reproduction.展开更多
Background:Canthariasis is a disease of humans caused by the infestation of beetle larvae.It is the second important insectal disease after myiasis.Several species of beetles are reported to cause the disease in gastr...Background:Canthariasis is a disease of humans caused by the infestation of beetle larvae.It is the second important insectal disease after myiasis.Several species of beetles are reported to cause the disease in gastrointestinal tract,urogenital system,nasal sinuses,ears and faces of mammals.The cigarette beetle Lasioderma serricorne is a widespread and destructive pest that usually feeds on tobacco,tea,beans,cereal grains,and animal and plant specimen.While there was no previous evidence of human infestation by this worm,we report the first case of L.serricorne infestation in a baby girl in China.Case presentation:Here the case,an eight-month-old baby girl with irritable feeling,rubbing eyes,history of contact with mud and eating oranges twice during five days before attendance,and having“worms”in her stool was admitted to the First Affiliated Hospital of Sun Yat-sen University,Guangzhou,China.The clinical examination revealed that the pulse rate,blood pressure and temperature were regular,and the examination of the head,neck,and chest were unremarkable.The stool specimens containing“worms”were sent to the Department of Parasitology,Zhongshan School of Medicine,Sun Yat-sen University.The worms were recovered,studied morphologically using naked eyes and anatomical lens,PCR analyzed targeting cytochrome oxidase subunit 1(COX1)and 18S rRNA genes,examined by sequence analyses of the PCR products and finally classified by phylogenetic analysis to identify their species.Based on the findings,the worms were diagnosed as the larvae of L.serricorne.Conclusion:This report implies that the baby had an infestation with the larvae of L.serricorne in the gastrointestine.During contact with mud or eating oranges by the girl,worm eggs were swallowed into the stomach and resisted gastric acid digestion which eventually hatched into larvae and caused canthariasis.The 8 months girl had underdeveloped immune system which might facilitate the disease.This report implicates that L.serricorne can infest human accidentally and cause canthariasis that may lead to severe damage to infant and older patient upon involvement of important organs of the body.The patients once diagnosed having canthariasis should be treated in time.展开更多
Lasioderma serricorne is known to be pest of tobacco, besides of attacking other products in storage. Anisopteromalus calandrae is an ectoparasitoid of coleopteran larvae also parasitizing the tobacco beetle. This stu...Lasioderma serricorne is known to be pest of tobacco, besides of attacking other products in storage. Anisopteromalus calandrae is an ectoparasitoid of coleopteran larvae also parasitizing the tobacco beetle. This study was aimed to evaluate the parasitism of A. calandrae on different densities of L. serricorne larvae grown in different diets and photoperiods, and to record the longevity and reproductive potential of A. calandrae. Individuals of L. serricorne were raised in three diets: wheat flour (F);wheat flour and brewer’s yeast (FY) and wheat flour and dried tobacco (FT). Different amounts of host larvae (10, 20, 50 and 100) for each diet were exposed to a couple of parasitoids. The same larval densities from diet F were exposed for 24 h to a couple of adult parasitoids maintained in three photoperiods (0:24, 12:12 and 24:0 - scotophase: photophase). The highest values of apparent parasitism were in the density of 50 larvae in the FY diet (96.34%) and 100 F (92.91%). There was no significant difference in the parameters in each photoperiod in all larval densities. However, the treatment in which hosts and parasitoids always remained in scotophase, was the one that had a significantly higher sex ratio. Females had longer longevity than males surviving for up to 25 days. On the fourth day of larvae exposure occurred, the maximum number of offspring generated. It is inferred that A. calandrae has potential to be used as a control agent for coleopterans that attack stored products.展开更多
基金This research was funded by the National Natural Science Foundation of China(32160637)the Project of China Tobacco Guizhou Industrial Co.,Ltd.(2017-25)+2 种基金the Special Project for Local(Guizhou)Science and Technology Development under the guidance of the Central Government(QKZYD[2022]4013)the Graduate Research Project of Guizhou Province(QJHYJSKYJJ[2021]194)the Program of Excellent Innovation Talents in Guizhou Province(20206003).
文摘High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable component of the insulin signaling pathway.Silencing of LsAkt severely hinders reproduction in Lasioderma serricorne,a stored product insect pest.However,the post-transcriptional pathway of LsAkt in L.serricorne remains unknown.This study identified 2 binding sites of miR-9c-5p and novel-mir50 in the coding sequences of LsAkt.The expression profiles of 2 microRNAs(miRNAs)and LsAkt displayed an opposite pattern during the adult stages.Luciferase reporter assay showed that novel-mir50 and miR-9c-5p could downregulate the expression of LsAkt.Overexpression of miR-9c-5p and novel-mir50 by injection of mimics inhibited the expression of LsAkt and reduced oviposition,decreased egg hatchability,and blocked ovarian development.It also decreased the expression of genes involved in ovarian development(LsVg and LsVgR)and the nutritional signaling pathway(LsTOR,LsS6K,and Ls4EBP),and reduced the phosphorylation of Akt.Conversely,injection of miR-9c-5p and novel-mir50 inhibitors induced the expressions of LsAkt,LsVg,LsVgR,LsTOR,LsS6K,and Ls4EBP,enhanced Akt phosphorylation level,and accelerated ovarian development.Injection of bovine insulin downregulated the expression of miR-9c-5p and novel-mir50 and upregulated the LsAkt expression.It also rescued the reproductive development defects associated with miR-9c-5p/novel-mir50 overexpression,forming a positive regulatory loop of insulin signaling.These results indicate that miR-9c-5p/novel-mir50 regulates the female reproduction of L.serricorne by targeting Akt in response to insulin signaling.The data also demonstrate the effects of the insulin/miRNA/Akt regulatory axis in insect reproduction.
基金supported by the National High Technology Research and Development Program of China(2015AA020934)National Natural Science Foundation of China(30972574)+4 种基金National Natural Science Foundation of China(81201309)China Postdoctoral Science Foundation(2011 M501363)Natural Science Foundation of Guangdong Province of China(S2012040007256)Foundation of Doctoral Program of Higher Education of China(20120171120049)the design of the study,collection,analysis and interpretation of data,and writing the manuscript.
文摘Background:Canthariasis is a disease of humans caused by the infestation of beetle larvae.It is the second important insectal disease after myiasis.Several species of beetles are reported to cause the disease in gastrointestinal tract,urogenital system,nasal sinuses,ears and faces of mammals.The cigarette beetle Lasioderma serricorne is a widespread and destructive pest that usually feeds on tobacco,tea,beans,cereal grains,and animal and plant specimen.While there was no previous evidence of human infestation by this worm,we report the first case of L.serricorne infestation in a baby girl in China.Case presentation:Here the case,an eight-month-old baby girl with irritable feeling,rubbing eyes,history of contact with mud and eating oranges twice during five days before attendance,and having“worms”in her stool was admitted to the First Affiliated Hospital of Sun Yat-sen University,Guangzhou,China.The clinical examination revealed that the pulse rate,blood pressure and temperature were regular,and the examination of the head,neck,and chest were unremarkable.The stool specimens containing“worms”were sent to the Department of Parasitology,Zhongshan School of Medicine,Sun Yat-sen University.The worms were recovered,studied morphologically using naked eyes and anatomical lens,PCR analyzed targeting cytochrome oxidase subunit 1(COX1)and 18S rRNA genes,examined by sequence analyses of the PCR products and finally classified by phylogenetic analysis to identify their species.Based on the findings,the worms were diagnosed as the larvae of L.serricorne.Conclusion:This report implies that the baby had an infestation with the larvae of L.serricorne in the gastrointestine.During contact with mud or eating oranges by the girl,worm eggs were swallowed into the stomach and resisted gastric acid digestion which eventually hatched into larvae and caused canthariasis.The 8 months girl had underdeveloped immune system which might facilitate the disease.This report implicates that L.serricorne can infest human accidentally and cause canthariasis that may lead to severe damage to infant and older patient upon involvement of important organs of the body.The patients once diagnosed having canthariasis should be treated in time.
基金the Conselho Nacional de Pesquisa—CNPq for financial support in DTI-C scholarships and masters scholarship
文摘Lasioderma serricorne is known to be pest of tobacco, besides of attacking other products in storage. Anisopteromalus calandrae is an ectoparasitoid of coleopteran larvae also parasitizing the tobacco beetle. This study was aimed to evaluate the parasitism of A. calandrae on different densities of L. serricorne larvae grown in different diets and photoperiods, and to record the longevity and reproductive potential of A. calandrae. Individuals of L. serricorne were raised in three diets: wheat flour (F);wheat flour and brewer’s yeast (FY) and wheat flour and dried tobacco (FT). Different amounts of host larvae (10, 20, 50 and 100) for each diet were exposed to a couple of parasitoids. The same larval densities from diet F were exposed for 24 h to a couple of adult parasitoids maintained in three photoperiods (0:24, 12:12 and 24:0 - scotophase: photophase). The highest values of apparent parasitism were in the density of 50 larvae in the FY diet (96.34%) and 100 F (92.91%). There was no significant difference in the parameters in each photoperiod in all larval densities. However, the treatment in which hosts and parasitoids always remained in scotophase, was the one that had a significantly higher sex ratio. Females had longer longevity than males surviving for up to 25 days. On the fourth day of larvae exposure occurred, the maximum number of offspring generated. It is inferred that A. calandrae has potential to be used as a control agent for coleopterans that attack stored products.
