The attractiveness of Cinnamomum camphora(Laurales: Lauraceae) blocks and essential oil to Lasioderma serricorne(Fabricius)(Coleoptera: Anobiidae) adults was investigated. The results showed that the behavioral respon...The attractiveness of Cinnamomum camphora(Laurales: Lauraceae) blocks and essential oil to Lasioderma serricorne(Fabricius)(Coleoptera: Anobiidae) adults was investigated. The results showed that the behavioral response values of L. serricorne adults to C. camphora blocks significantly increased with increasing doses above the dose of 0.8 g. The highest behavioral response values of L. serricorne adults were 93.33% to C. camphora blocks and 81.67% to C. camphora essential oil, respectively. C. camphora blocks had stronger attractiveness to L. serricorne adults than C. camphora oil. These results demonstrate that the effectiveness to control L. serricorne in practice of C. camphora blocks and C. camphora oil deserve to be further investigated.展开更多
High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable component of the insulin signaling pathway.Silencing of Ls...High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable component of the insulin signaling pathway.Silencing of LsAkt severely hinders reproduction in Lasioderma serricorne,a stored product insect pest.However,the post-transcriptional pathway of LsAkt in L.serricorne remains unknown.This study identified 2 binding sites of miR-9c-5p and novel-mir50 in the coding sequences of LsAkt.The expression profiles of 2 microRNAs(miRNAs)and LsAkt displayed an opposite pattern during the adult stages.Luciferase reporter assay showed that novel-mir50 and miR-9c-5p could downregulate the expression of LsAkt.Overexpression of miR-9c-5p and novel-mir50 by injection of mimics inhibited the expression of LsAkt and reduced oviposition,decreased egg hatchability,and blocked ovarian development.It also decreased the expression of genes involved in ovarian development(LsVg and LsVgR)and the nutritional signaling pathway(LsTOR,LsS6K,and Ls4EBP),and reduced the phosphorylation of Akt.Conversely,injection of miR-9c-5p and novel-mir50 inhibitors induced the expressions of LsAkt,LsVg,LsVgR,LsTOR,LsS6K,and Ls4EBP,enhanced Akt phosphorylation level,and accelerated ovarian development.Injection of bovine insulin downregulated the expression of miR-9c-5p and novel-mir50 and upregulated the LsAkt expression.It also rescued the reproductive development defects associated with miR-9c-5p/novel-mir50 overexpression,forming a positive regulatory loop of insulin signaling.These results indicate that miR-9c-5p/novel-mir50 regulates the female reproduction of L.serricorne by targeting Akt in response to insulin signaling.The data also demonstrate the effects of the insulin/miRNA/Akt regulatory axis in insect reproduction.展开更多
Antimicrobial peptides(AMPs)in insects are endogenous peptides that are effector components of the innate defense system of the insect.AMPs may serve as antimicrobial agents because of their small molecular weight and...Antimicrobial peptides(AMPs)in insects are endogenous peptides that are effector components of the innate defense system of the insect.AMPs may serve as antimicrobial agents because of their small molecular weight and broad-spectrum antimicrobial activity.In this study,we performed transcriptome analysis of cigarette beetle(Lasioderma serricorne)larvae,parasitized by the ectoparasitic wasp,Anisopteromalus calandrae.Several AMP genes were significantly upregulated following A.calandrae parasitism,postulating the hypothesis that the parasitization enhanced the host's resistance against pathogenic microorganisms through the regulation of host AMP genes.Specifically,3 AMP genes(LsDef1,LsDef2,and LsCole)were significantly upregulated and we studied their immune function in L.serricorne.Immune challenge and functional analysis showed that LsCole was responsible for the immune response against Gram-negative and Gram-positive bacteria,while LsDef1 and LsDef2 were involved in insect defense against Gram-positive bacteria.Purified recombinant LsCole exhibited antimicrobial activities against the Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Staphylococcus aureus.LsDef2 showed an antibacterial effect against S.aureus.LsCole and LsDef2 exhibited antibiofilm activity against S.aureus.The 2 AMPs disrupted cell membranes and caused leakage of S.aureus cell contents.The results indicated that the 3 AMPs in L.serricorne are involved in the innate immunity of this pest insect.These AMPs may have potential as antimicrobial agents for bacterial infection chemotherapy.Hence,data are discussed in relation to new control strategies with greater biosafety against pest insects with use of microbial biocontrol agents in combination with RNA interference against the insect's defensive AMP genes.展开更多
Background:Canthariasis is a disease of humans caused by the infestation of beetle larvae.It is the second important insectal disease after myiasis.Several species of beetles are reported to cause the disease in gastr...Background:Canthariasis is a disease of humans caused by the infestation of beetle larvae.It is the second important insectal disease after myiasis.Several species of beetles are reported to cause the disease in gastrointestinal tract,urogenital system,nasal sinuses,ears and faces of mammals.The cigarette beetle Lasioderma serricorne is a widespread and destructive pest that usually feeds on tobacco,tea,beans,cereal grains,and animal and plant specimen.While there was no previous evidence of human infestation by this worm,we report the first case of L.serricorne infestation in a baby girl in China.Case presentation:Here the case,an eight-month-old baby girl with irritable feeling,rubbing eyes,history of contact with mud and eating oranges twice during five days before attendance,and having“worms”in her stool was admitted to the First Affiliated Hospital of Sun Yat-sen University,Guangzhou,China.The clinical examination revealed that the pulse rate,blood pressure and temperature were regular,and the examination of the head,neck,and chest were unremarkable.The stool specimens containing“worms”were sent to the Department of Parasitology,Zhongshan School of Medicine,Sun Yat-sen University.The worms were recovered,studied morphologically using naked eyes and anatomical lens,PCR analyzed targeting cytochrome oxidase subunit 1(COX1)and 18S rRNA genes,examined by sequence analyses of the PCR products and finally classified by phylogenetic analysis to identify their species.Based on the findings,the worms were diagnosed as the larvae of L.serricorne.Conclusion:This report implies that the baby had an infestation with the larvae of L.serricorne in the gastrointestine.During contact with mud or eating oranges by the girl,worm eggs were swallowed into the stomach and resisted gastric acid digestion which eventually hatched into larvae and caused canthariasis.The 8 months girl had underdeveloped immune system which might facilitate the disease.This report implicates that L.serricorne can infest human accidentally and cause canthariasis that may lead to severe damage to infant and older patient upon involvement of important organs of the body.The patients once diagnosed having canthariasis should be treated in time.展开更多
基金Supported by National Program on Key R&D Project(No.2017YFC1600804)Key Technologies R&D Program of the Education Department of Henan Province(No.16A210017)Province Key Laboratory of Transformation and Utilization of Cereal Resource,Henan University of Technology
文摘The attractiveness of Cinnamomum camphora(Laurales: Lauraceae) blocks and essential oil to Lasioderma serricorne(Fabricius)(Coleoptera: Anobiidae) adults was investigated. The results showed that the behavioral response values of L. serricorne adults to C. camphora blocks significantly increased with increasing doses above the dose of 0.8 g. The highest behavioral response values of L. serricorne adults were 93.33% to C. camphora blocks and 81.67% to C. camphora essential oil, respectively. C. camphora blocks had stronger attractiveness to L. serricorne adults than C. camphora oil. These results demonstrate that the effectiveness to control L. serricorne in practice of C. camphora blocks and C. camphora oil deserve to be further investigated.
基金This research was funded by the National Natural Science Foundation of China(32160637)the Project of China Tobacco Guizhou Industrial Co.,Ltd.(2017-25)+2 种基金the Special Project for Local(Guizhou)Science and Technology Development under the guidance of the Central Government(QKZYD[2022]4013)the Graduate Research Project of Guizhou Province(QJHYJSKYJJ[2021]194)the Program of Excellent Innovation Talents in Guizhou Province(20206003).
文摘High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable component of the insulin signaling pathway.Silencing of LsAkt severely hinders reproduction in Lasioderma serricorne,a stored product insect pest.However,the post-transcriptional pathway of LsAkt in L.serricorne remains unknown.This study identified 2 binding sites of miR-9c-5p and novel-mir50 in the coding sequences of LsAkt.The expression profiles of 2 microRNAs(miRNAs)and LsAkt displayed an opposite pattern during the adult stages.Luciferase reporter assay showed that novel-mir50 and miR-9c-5p could downregulate the expression of LsAkt.Overexpression of miR-9c-5p and novel-mir50 by injection of mimics inhibited the expression of LsAkt and reduced oviposition,decreased egg hatchability,and blocked ovarian development.It also decreased the expression of genes involved in ovarian development(LsVg and LsVgR)and the nutritional signaling pathway(LsTOR,LsS6K,and Ls4EBP),and reduced the phosphorylation of Akt.Conversely,injection of miR-9c-5p and novel-mir50 inhibitors induced the expressions of LsAkt,LsVg,LsVgR,LsTOR,LsS6K,and Ls4EBP,enhanced Akt phosphorylation level,and accelerated ovarian development.Injection of bovine insulin downregulated the expression of miR-9c-5p and novel-mir50 and upregulated the LsAkt expression.It also rescued the reproductive development defects associated with miR-9c-5p/novel-mir50 overexpression,forming a positive regulatory loop of insulin signaling.These results indicate that miR-9c-5p/novel-mir50 regulates the female reproduction of L.serricorne by targeting Akt in response to insulin signaling.The data also demonstrate the effects of the insulin/miRNA/Akt regulatory axis in insect reproduction.
基金supported by the Program of Excellent Innovation Talents,Guizhou Province,China(Grant number 20206003-2)the Guizhou Province Graduate Research Fund YJSKYJJ[2021]053the Special Project for Science and Technology Development of Local(Guizhou)under the Guidance of the Central Government(QKZYD[2022]4013).
文摘Antimicrobial peptides(AMPs)in insects are endogenous peptides that are effector components of the innate defense system of the insect.AMPs may serve as antimicrobial agents because of their small molecular weight and broad-spectrum antimicrobial activity.In this study,we performed transcriptome analysis of cigarette beetle(Lasioderma serricorne)larvae,parasitized by the ectoparasitic wasp,Anisopteromalus calandrae.Several AMP genes were significantly upregulated following A.calandrae parasitism,postulating the hypothesis that the parasitization enhanced the host's resistance against pathogenic microorganisms through the regulation of host AMP genes.Specifically,3 AMP genes(LsDef1,LsDef2,and LsCole)were significantly upregulated and we studied their immune function in L.serricorne.Immune challenge and functional analysis showed that LsCole was responsible for the immune response against Gram-negative and Gram-positive bacteria,while LsDef1 and LsDef2 were involved in insect defense against Gram-positive bacteria.Purified recombinant LsCole exhibited antimicrobial activities against the Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Staphylococcus aureus.LsDef2 showed an antibacterial effect against S.aureus.LsCole and LsDef2 exhibited antibiofilm activity against S.aureus.The 2 AMPs disrupted cell membranes and caused leakage of S.aureus cell contents.The results indicated that the 3 AMPs in L.serricorne are involved in the innate immunity of this pest insect.These AMPs may have potential as antimicrobial agents for bacterial infection chemotherapy.Hence,data are discussed in relation to new control strategies with greater biosafety against pest insects with use of microbial biocontrol agents in combination with RNA interference against the insect's defensive AMP genes.
基金supported by the National High Technology Research and Development Program of China(2015AA020934)National Natural Science Foundation of China(30972574)+4 种基金National Natural Science Foundation of China(81201309)China Postdoctoral Science Foundation(2011 M501363)Natural Science Foundation of Guangdong Province of China(S2012040007256)Foundation of Doctoral Program of Higher Education of China(20120171120049)the design of the study,collection,analysis and interpretation of data,and writing the manuscript.
文摘Background:Canthariasis is a disease of humans caused by the infestation of beetle larvae.It is the second important insectal disease after myiasis.Several species of beetles are reported to cause the disease in gastrointestinal tract,urogenital system,nasal sinuses,ears and faces of mammals.The cigarette beetle Lasioderma serricorne is a widespread and destructive pest that usually feeds on tobacco,tea,beans,cereal grains,and animal and plant specimen.While there was no previous evidence of human infestation by this worm,we report the first case of L.serricorne infestation in a baby girl in China.Case presentation:Here the case,an eight-month-old baby girl with irritable feeling,rubbing eyes,history of contact with mud and eating oranges twice during five days before attendance,and having“worms”in her stool was admitted to the First Affiliated Hospital of Sun Yat-sen University,Guangzhou,China.The clinical examination revealed that the pulse rate,blood pressure and temperature were regular,and the examination of the head,neck,and chest were unremarkable.The stool specimens containing“worms”were sent to the Department of Parasitology,Zhongshan School of Medicine,Sun Yat-sen University.The worms were recovered,studied morphologically using naked eyes and anatomical lens,PCR analyzed targeting cytochrome oxidase subunit 1(COX1)and 18S rRNA genes,examined by sequence analyses of the PCR products and finally classified by phylogenetic analysis to identify their species.Based on the findings,the worms were diagnosed as the larvae of L.serricorne.Conclusion:This report implies that the baby had an infestation with the larvae of L.serricorne in the gastrointestine.During contact with mud or eating oranges by the girl,worm eggs were swallowed into the stomach and resisted gastric acid digestion which eventually hatched into larvae and caused canthariasis.The 8 months girl had underdeveloped immune system which might facilitate the disease.This report implicates that L.serricorne can infest human accidentally and cause canthariasis that may lead to severe damage to infant and older patient upon involvement of important organs of the body.The patients once diagnosed having canthariasis should be treated in time.
