A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cD...A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5' untranslated region (UTR) of 47 bp, a 3' UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that of zebrafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosylation site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver.展开更多
The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, t...The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 (SPsn25) by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831bp contains a CDS of 615 bp, coding 204 amino acid residues, and a 5′UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.展开更多
The metabolic rate of Japanese sea bass,Lateolabrax japonicus (C & V), was estimated in laboratory at temperature 25.2±0.5°C. The fresh weight of the fish was 4.64–52.28 g (average of 17.81±0.33 g)...The metabolic rate of Japanese sea bass,Lateolabrax japonicus (C & V), was estimated in laboratory at temperature 25.2±0.5°C. The fresh weight of the fish was 4.64–52.28 g (average of 17.81±0.33 g). The routine metabolism was related to body weight by the exponential equation:R r =14.966W 0.74 (r=0.934). The rate of feeding metabolism increased linearly with food consumption. Feeding metabolic rate was 1.8–2.4 times the routine metabolic rate. Key words routine metabolism - feeding metabolism - Lateolabrax japonicus Contribution No. 4021 from the Institute of Oceanology, Chinese Academy of Sciences展开更多
Objectives:The nutrient rich sea bass is prone to oxidation of lipid and protein during refrigeration.Materials and Methods:The research was to investigate the effect of different concentrations of Melissa officinalis...Objectives:The nutrient rich sea bass is prone to oxidation of lipid and protein during refrigeration.Materials and Methods:The research was to investigate the effect of different concentrations of Melissa officinalis L.essential oil(MOEO)nanoemulsions on myofibrillar protein(MP)and lipid oxidation in sea bass(Lateolabrax japonicus)during refrigeration at 4°C.Results:The results of thiobarbituric acid reactive substances and mitochondrial membrane potential showed that carboxymethyl chitosan/locust bean gum active coating solutions incorporating 2%MOEO nanoemulsions(C/L-2M)was the most effective in inhibiting lipid oxidation that occurred in sea bass under attack by reactive oxygen species.Low-field nuclear magnetic resonance results showed that C/L-2M maximally slowed the conversion of bound water to free water during storage.The oxidation of lipids and MP disrupted the secondary and tertiary conformations of MP and accelerated protein aggregation and degradation.Conclusions:C/L-2M slowed the oxidation of lipids and proteins by inhibiting the oxidation of reactive oxygen species.C/L-2M is a very promising preservative emulsion for the preservation of sea bass.展开更多
This study applied in vivo and in vitro methods to investigate the effect of dietary N-carbamoylglutamate(NCG)on lipid metabolism,inflammation and apoptosis related-gene expression in visceral adipose tissue and isola...This study applied in vivo and in vitro methods to investigate the effect of dietary N-carbamoylglutamate(NCG)on lipid metabolism,inflammation and apoptosis related-gene expression in visceral adipose tissue and isolated adipocytes of Japanese seabass(Lateolabrax japonicus).A basal diet and a test diet supplemented with 720 mg/kg NCG were fed to the fish for 10 weeks.During the growth trial,no mortality and no significant differences in growth performance were observed in fish between the 2 groups(P>0.05).Plasma Arg content and mRNA level of argininosuccinate synthetase(ASS)in adipose tissue were significantly increased,which indicated that NCG inclusion promoted endogenous Arg synthesis.Thereafter,the potential effects of NCG treatment on lipid metabolism-related genes expression were studied through in vivo and in vitro methods.In the present study,we successfully established a primary adipocytes culture system and isolated pre-adipocytes in vitro of Japanese seabass for the first time.Both the results in vivo and in vitro showed that NCG treatment decreased the mRNA levels of genes related to adipogenesis(fatty acid synthase,FASN),cholesterol synthesis(3-hydroxy-3-methylglutaryl-CoA reductase,HMGCR)and fat deposition(lipoprotein lipase[LPL]and leptin),which revealed the underlying mechanism of NCG on reducing fat deposition.The results of this study demonstrated that NCG inclusion reduced the expression of inflammatory and apoptosis cytokines markedly in vivo and in vitro.In conclusion,NCG did exert beneficial effects on ameliorating adipo-genesis,inflammation and apoptosis via promoting Arg endogenous synthesis in Japanese seabass.展开更多
To investigate the effect of feed protein levels on growth and feed utilization of juvenile Lateolabraxjaponicus, fish meal was used as the major protein source to prepare five isoenergetic experimental feeds containi...To investigate the effect of feed protein levels on growth and feed utilization of juvenile Lateolabraxjaponicus, fish meal was used as the major protein source to prepare five isoenergetic experimental feeds containing 35.0%, 37.5%, 40.0%, 42.5% and 45.0% protein, respectively. Juvenile L. japonicus with the initial average body weight of （84.81 ±0.92） g were fed for 75 d to determine the best protein level for juvenile L. japonicus. The results showed that the relative weight gain rate, specific growth rate and feed conversion ratio in the group containing 39.85% protein were extremely higher than those in the groups containing 34.76% and 37.54% protein （ P 〈 0.01 ）, but had no significant difference with that in the group containing 42.34% protein （ P 〉0.05）. Protein efficiency ratio in the group containing 39.85% protein was also significantly higher than those in the groups containing 34.76%, 42.34% and 45.03% protein （ P 〈0.05）, while had no significant difference with that in the group containing 37.54% protein（ P 〉0.05）. In this experiment, based on quadratic model regression analysis of specific growth rate and protein efficiency ratio, growth performance and feed utilization rate of L. japonicus was the best when the protein content of feed was from 38.87% to 41.50%.展开更多
基金supported by the“863"Prijetof China under contract Nos 2001AA628180 and 2002AA626020.
文摘A homologue of the lower vertebrates translationally controlled tumor protein (TCTP) was cloned from the marine fish Japanese sea perch (Lateolabrax japonicus) by the technology of homology cloning. The full-length cDNA sequence of the sea perch TCTP gene contained a 5' untranslated region (UTR) of 47 bp, a 3' UTR of 433 bp, and a putative open reading frame (ORF) of 510 bp encoding a polypeptide of 170 amino acids. The deduced amino acid sequence of the sea perch TCTP gene showed a high similarity to that of zebrafish, rohu, rabbit, chicken and human. Sequence analysis revealed there were a signature sequence of TCTP family, an N-glycosylation site, and five Casein kinase phosphorylation sites in the sea perch TCTP. The temporal expression of TCTP genes in healthy and lipopolysaccharide (LPS) challenged fishes was measured by semi-quantitative reverse transcription-PCR (RT-PCR). The results indicated that LPS could up-regulate the expression of sea perch TCTP in the examined tissues, including head-kidney, spleen and liver.
基金the NSFC (No.40476060)Hi-Tech Research and Development Program of China (No. 2002AA629120)
文摘The t-SNARE protein SNAP-25 (synaptosome-associated protein of 25 kDa) plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 (SPsn25) by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831bp contains a CDS of 615 bp, coding 204 amino acid residues, and a 5′UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.
文摘The metabolic rate of Japanese sea bass,Lateolabrax japonicus (C & V), was estimated in laboratory at temperature 25.2±0.5°C. The fresh weight of the fish was 4.64–52.28 g (average of 17.81±0.33 g). The routine metabolism was related to body weight by the exponential equation:R r =14.966W 0.74 (r=0.934). The rate of feeding metabolism increased linearly with food consumption. Feeding metabolic rate was 1.8–2.4 times the routine metabolic rate. Key words routine metabolism - feeding metabolism - Lateolabrax japonicus Contribution No. 4021 from the Institute of Oceanology, Chinese Academy of Sciences
基金supported by the China Agriculture Research System of MOF and MARA(CARS-47)the Shanghai Municipal Science and Technology Project to Enhance the Capabilities of the Platform(Nos.20DZ2292200 and 19DZ2284000),China.
文摘Objectives:The nutrient rich sea bass is prone to oxidation of lipid and protein during refrigeration.Materials and Methods:The research was to investigate the effect of different concentrations of Melissa officinalis L.essential oil(MOEO)nanoemulsions on myofibrillar protein(MP)and lipid oxidation in sea bass(Lateolabrax japonicus)during refrigeration at 4°C.Results:The results of thiobarbituric acid reactive substances and mitochondrial membrane potential showed that carboxymethyl chitosan/locust bean gum active coating solutions incorporating 2%MOEO nanoemulsions(C/L-2M)was the most effective in inhibiting lipid oxidation that occurred in sea bass under attack by reactive oxygen species.Low-field nuclear magnetic resonance results showed that C/L-2M maximally slowed the conversion of bound water to free water during storage.The oxidation of lipids and MP disrupted the secondary and tertiary conformations of MP and accelerated protein aggregation and degradation.Conclusions:C/L-2M slowed the oxidation of lipids and proteins by inhibiting the oxidation of reactive oxygen species.C/L-2M is a very promising preservative emulsion for the preservation of sea bass.
基金supported by the National Key R&D Program of China(2019YFD0900200,2018YFD0900400)the Agricultural Science and Technology Innovation Program(CAAS-ASTIP-2017-FRI-08)+2 种基金Beijing Technology System for Sturgeon and Salmonids(BAIC08-2021)National Natural Science Foundation of China(31902382)Natural Science Foundation of Beijing(6204047)
文摘This study applied in vivo and in vitro methods to investigate the effect of dietary N-carbamoylglutamate(NCG)on lipid metabolism,inflammation and apoptosis related-gene expression in visceral adipose tissue and isolated adipocytes of Japanese seabass(Lateolabrax japonicus).A basal diet and a test diet supplemented with 720 mg/kg NCG were fed to the fish for 10 weeks.During the growth trial,no mortality and no significant differences in growth performance were observed in fish between the 2 groups(P>0.05).Plasma Arg content and mRNA level of argininosuccinate synthetase(ASS)in adipose tissue were significantly increased,which indicated that NCG inclusion promoted endogenous Arg synthesis.Thereafter,the potential effects of NCG treatment on lipid metabolism-related genes expression were studied through in vivo and in vitro methods.In the present study,we successfully established a primary adipocytes culture system and isolated pre-adipocytes in vitro of Japanese seabass for the first time.Both the results in vivo and in vitro showed that NCG treatment decreased the mRNA levels of genes related to adipogenesis(fatty acid synthase,FASN),cholesterol synthesis(3-hydroxy-3-methylglutaryl-CoA reductase,HMGCR)and fat deposition(lipoprotein lipase[LPL]and leptin),which revealed the underlying mechanism of NCG on reducing fat deposition.The results of this study demonstrated that NCG inclusion reduced the expression of inflammatory and apoptosis cytokines markedly in vivo and in vitro.In conclusion,NCG did exert beneficial effects on ameliorating adipo-genesis,inflammation and apoptosis via promoting Arg endogenous synthesis in Japanese seabass.
基金Supported by Science and Technology Spark Program of Fujian Province(2011S0015)
文摘To investigate the effect of feed protein levels on growth and feed utilization of juvenile Lateolabraxjaponicus, fish meal was used as the major protein source to prepare five isoenergetic experimental feeds containing 35.0%, 37.5%, 40.0%, 42.5% and 45.0% protein, respectively. Juvenile L. japonicus with the initial average body weight of （84.81 ±0.92） g were fed for 75 d to determine the best protein level for juvenile L. japonicus. The results showed that the relative weight gain rate, specific growth rate and feed conversion ratio in the group containing 39.85% protein were extremely higher than those in the groups containing 34.76% and 37.54% protein （ P 〈 0.01 ）, but had no significant difference with that in the group containing 42.34% protein （ P 〉0.05）. Protein efficiency ratio in the group containing 39.85% protein was also significantly higher than those in the groups containing 34.76%, 42.34% and 45.03% protein （ P 〈0.05）, while had no significant difference with that in the group containing 37.54% protein（ P 〉0.05）. In this experiment, based on quadratic model regression analysis of specific growth rate and protein efficiency ratio, growth performance and feed utilization rate of L. japonicus was the best when the protein content of feed was from 38.87% to 41.50%.
