Western diet(rich in highly refined sugar and fat)can induce a range of metabolic dysfunctions in animals and humans,including neuroinflammation and cognitive function decline.Neuroinflammation and cognitive impairmen...Western diet(rich in highly refined sugar and fat)can induce a range of metabolic dysfunctions in animals and humans,including neuroinflammation and cognitive function decline.Neuroinflammation and cognitive impairment,two critical pathological characteristics of Alzheimer’s disease,have been closely associated with microbial alteration via the gut-brain axis.Thus,the present study aimed to investigate the influence of 2-O-β-D-glucopyranosyl-L-ascorbic acid(AA-2βG)isolated from the fruits of Lycium barbarum on preventing the high-fructose diet(HFrD)induced neuroinflammation in mice.It was found that AA-2βG prevented HFr D-induced cognitive deficits.AA-2βG also predominantly enhanced the gut barrier integrity,decreased lipopolysaccharide entry into the circulation,which subsequently countered the activation of glial cells and neuroinflammatory response.These beneficial effects were transmissible by horizontal fecal microbiome transplantation,transferring from AA-2βG fed mice to HFr D fed mice.Additionally,AA-2βG exerted neuroprotective effects involving the enrichment of Lactobacillus and Akkermansia,potentially beneficial intestinal bacteria.The present study provided the evidence that AA-2βG could improve indices of cognition and neuroinflammmation via modulating gut dybiosis and preventing leaky gut.As a potential functional food ingredient,AA-2βG may be applied to attenuate neuroinflammation associated with Western-style diets.展开更多
BACKGROUND The gut–liver axis and bacterial translocation are important in cirrhosis,but there is no available universal biomarker of cellular bacterial translocation,for which presepsin may be a candidate.AIM To eva...BACKGROUND The gut–liver axis and bacterial translocation are important in cirrhosis,but there is no available universal biomarker of cellular bacterial translocation,for which presepsin may be a candidate.AIM To evaluate the relationship of the blood presepsin levels with the state of the gut microbiota in cirrhosis in the absence of obvious infection.METHODS This study included 48 patients with Child–Pugh cirrhosis classes B and C and 15 healthy controls.The fecal microbiome was assessed using 16S rRNA gene sequencing.Plasma levels of presepsin were measured.A total of 22 patients received a probiotic(Saccharomyces boulardii)for 3 months.RESULTS Presepsin levels were higher in patients with cirrhosis than in healthy individuals[342(91-2875)vs 120(102-141)pg/mL;P=0.048].Patients with elevated presepsin levels accounted for 56.3%of all included patients.They had lower levels of serum albumin and higher levels of serum total bilirubin and overall severity of cirrhosis as assessed using the Child–Pugh scale.Patients with elevated presepsin levels had an increased abundance of the main taxa responsible for bacterial translocation,namely Bacilli and Proteobacteria(including the main class Gammaproteobacteria and the minor taxa Xanthobacteraceae and Stenotrophomonas),and a low abundance of bacteria from the family Lachnospiraceae(including the minor genus Fusicatenibacter),which produce short-chain fatty acids that have a positive effect on intestinal barrier function.The presepsin level directly correlated with the relative abundance of Bacilli,Proteobacteria,and inversely correlated with the abundance of Lachnospiraceae and Propionibacteriaceae.After 3 months of taking the probiotic,the severity of cirrhosis on the Child–Pugh scale decreased significantly only in the group with elevated presepsin levels[from 9(8-11)to 7(6-9);P=0.004],while there were no significant changes in the group with normal presepsin levels[from 8(7-8)to 7(6-8);P=0.123].A high level of presepsin before the prescription of the probiotic was an independent predictor of a greater decrease in Child–Pugh scores(P=0.046),as well as a higher level of the Child–Pugh scale(P=0.042),but not the C-reactive protein level(P=0.679)according to multivariate linear regression analysis.CONCLUSION The level of presepsin directly correlates with the abundance in the gut microbiota of the main taxa that are substrates of bacterial translocation in cirrhosis.This biomarker,in the absence of obvious infection,seems important for assessing the state of the gut–liver axis in cirrhosis and deciding on therapy targeted at the gut microbiota in this disease.展开更多
为了提高表情识别率并降低表情识别的功耗,提出一种基于改进深度残差网络的表情识别方法。残差学习在解决深度卷积神经网络退化问题、使网络层次大幅加深的同时,进一步增加了网络的功耗。为此,引入具有生物真实性的激活函数来代替已有...为了提高表情识别率并降低表情识别的功耗,提出一种基于改进深度残差网络的表情识别方法。残差学习在解决深度卷积神经网络退化问题、使网络层次大幅加深的同时,进一步增加了网络的功耗。为此,引入具有生物真实性的激活函数来代替已有的整流线性单元(Rectified Linear Units,ReLU)函数,并将其作为卷积层激活函数对深度残差网络进行改进。该方法不仅提高了残差网络的精度,而且训练出的网络权重可直接作为与该深度残差网络具有相同结构的深度脉冲神经网络的权重。将该深度脉冲神经网络部署在类脑硬件上时,其能够以较高的识别率和较低的能耗进行表情识别。展开更多
A "leaky gut" may be the cutting edge for the passage of toxins, antigens or bacteria into the body, and may play a pathogenic role in advanced liver cirrhosis and its complications. Plasma endotoxin levels ...A "leaky gut" may be the cutting edge for the passage of toxins, antigens or bacteria into the body, and may play a pathogenic role in advanced liver cirrhosis and its complications. Plasma endotoxin levels have been admitted as a surrogate marker of bacterial translocation and close relations of endotoxemia to hyperdynamic circulation, portal hypertension, renal, cardiac, pulmonary and coagulation disturbances have been reported. Bacterial overgrowth, increased intestinal permeability, failure to inactivate endotoxin,activated innate immunity are all likely to play a role in the pathological states of bacterial translocation. Therapeutic approach by management of the gut-liver axis by antibiotics, probiotics, synbiotics, prebiotics and their combinations may improve the clinical course of cirrhotic patients. Special concern should be paid on anti-endotoxin treatment. Adequate management of the gut-liver axis may be effective for prevention of liver cirrhosis itself by inhibiting the progression of fibrosis.展开更多
针对神经网络结构的特征提取能力不足以及在包含复杂图像特征的数据集上分类准确率不高的问题,本文提出了一种对MobileNet神经网络的改进策略(L-MobileNet)。将原标准卷积形式替换为深度可分离卷积形式,并将深度卷积层得到的特征图执行...针对神经网络结构的特征提取能力不足以及在包含复杂图像特征的数据集上分类准确率不高的问题,本文提出了一种对MobileNet神经网络的改进策略(L-MobileNet)。将原标准卷积形式替换为深度可分离卷积形式,并将深度卷积层得到的特征图执行取反操作,通过深度卷积融合层传递至下一层;采用Leaky ReLU激活函数代替原ReLU激活函数来保留图像中更多的正负特征信息,并加入类残差结构避免梯度弥散现象。与6种方法进行对比,实验结果表明:L-MobileNet在数据集Cifar-10、Cifar-100(coarse)、Cifar-100(fine)和Dogs vs Cats上平均准确率和最高准确率都取得了最佳结果。展开更多
基金the financial support from the Key Research and Development Program of Ningxia Hui Autonomous Region of China(2021BEF02008)the National Natural Science Foundation of China(32272330)the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘Western diet(rich in highly refined sugar and fat)can induce a range of metabolic dysfunctions in animals and humans,including neuroinflammation and cognitive function decline.Neuroinflammation and cognitive impairment,two critical pathological characteristics of Alzheimer’s disease,have been closely associated with microbial alteration via the gut-brain axis.Thus,the present study aimed to investigate the influence of 2-O-β-D-glucopyranosyl-L-ascorbic acid(AA-2βG)isolated from the fruits of Lycium barbarum on preventing the high-fructose diet(HFrD)induced neuroinflammation in mice.It was found that AA-2βG prevented HFr D-induced cognitive deficits.AA-2βG also predominantly enhanced the gut barrier integrity,decreased lipopolysaccharide entry into the circulation,which subsequently countered the activation of glial cells and neuroinflammatory response.These beneficial effects were transmissible by horizontal fecal microbiome transplantation,transferring from AA-2βG fed mice to HFr D fed mice.Additionally,AA-2βG exerted neuroprotective effects involving the enrichment of Lactobacillus and Akkermansia,potentially beneficial intestinal bacteria.The present study provided the evidence that AA-2βG could improve indices of cognition and neuroinflammmation via modulating gut dybiosis and preventing leaky gut.As a potential functional food ingredient,AA-2βG may be applied to attenuate neuroinflammation associated with Western-style diets.
基金approved by the Ethics Committee of Sechenov University and performed in accordance with the Declaration of Helsinki.
文摘BACKGROUND The gut–liver axis and bacterial translocation are important in cirrhosis,but there is no available universal biomarker of cellular bacterial translocation,for which presepsin may be a candidate.AIM To evaluate the relationship of the blood presepsin levels with the state of the gut microbiota in cirrhosis in the absence of obvious infection.METHODS This study included 48 patients with Child–Pugh cirrhosis classes B and C and 15 healthy controls.The fecal microbiome was assessed using 16S rRNA gene sequencing.Plasma levels of presepsin were measured.A total of 22 patients received a probiotic(Saccharomyces boulardii)for 3 months.RESULTS Presepsin levels were higher in patients with cirrhosis than in healthy individuals[342(91-2875)vs 120(102-141)pg/mL;P=0.048].Patients with elevated presepsin levels accounted for 56.3%of all included patients.They had lower levels of serum albumin and higher levels of serum total bilirubin and overall severity of cirrhosis as assessed using the Child–Pugh scale.Patients with elevated presepsin levels had an increased abundance of the main taxa responsible for bacterial translocation,namely Bacilli and Proteobacteria(including the main class Gammaproteobacteria and the minor taxa Xanthobacteraceae and Stenotrophomonas),and a low abundance of bacteria from the family Lachnospiraceae(including the minor genus Fusicatenibacter),which produce short-chain fatty acids that have a positive effect on intestinal barrier function.The presepsin level directly correlated with the relative abundance of Bacilli,Proteobacteria,and inversely correlated with the abundance of Lachnospiraceae and Propionibacteriaceae.After 3 months of taking the probiotic,the severity of cirrhosis on the Child–Pugh scale decreased significantly only in the group with elevated presepsin levels[from 9(8-11)to 7(6-9);P=0.004],while there were no significant changes in the group with normal presepsin levels[from 8(7-8)to 7(6-8);P=0.123].A high level of presepsin before the prescription of the probiotic was an independent predictor of a greater decrease in Child–Pugh scores(P=0.046),as well as a higher level of the Child–Pugh scale(P=0.042),but not the C-reactive protein level(P=0.679)according to multivariate linear regression analysis.CONCLUSION The level of presepsin directly correlates with the abundance in the gut microbiota of the main taxa that are substrates of bacterial translocation in cirrhosis.This biomarker,in the absence of obvious infection,seems important for assessing the state of the gut–liver axis in cirrhosis and deciding on therapy targeted at the gut microbiota in this disease.
文摘为了提高表情识别率并降低表情识别的功耗,提出一种基于改进深度残差网络的表情识别方法。残差学习在解决深度卷积神经网络退化问题、使网络层次大幅加深的同时,进一步增加了网络的功耗。为此,引入具有生物真实性的激活函数来代替已有的整流线性单元(Rectified Linear Units,ReLU)函数,并将其作为卷积层激活函数对深度残差网络进行改进。该方法不仅提高了残差网络的精度,而且训练出的网络权重可直接作为与该深度残差网络具有相同结构的深度脉冲神经网络的权重。将该深度脉冲神经网络部署在类脑硬件上时,其能够以较高的识别率和较低的能耗进行表情识别。
文摘A "leaky gut" may be the cutting edge for the passage of toxins, antigens or bacteria into the body, and may play a pathogenic role in advanced liver cirrhosis and its complications. Plasma endotoxin levels have been admitted as a surrogate marker of bacterial translocation and close relations of endotoxemia to hyperdynamic circulation, portal hypertension, renal, cardiac, pulmonary and coagulation disturbances have been reported. Bacterial overgrowth, increased intestinal permeability, failure to inactivate endotoxin,activated innate immunity are all likely to play a role in the pathological states of bacterial translocation. Therapeutic approach by management of the gut-liver axis by antibiotics, probiotics, synbiotics, prebiotics and their combinations may improve the clinical course of cirrhotic patients. Special concern should be paid on anti-endotoxin treatment. Adequate management of the gut-liver axis may be effective for prevention of liver cirrhosis itself by inhibiting the progression of fibrosis.
文摘针对神经网络结构的特征提取能力不足以及在包含复杂图像特征的数据集上分类准确率不高的问题,本文提出了一种对MobileNet神经网络的改进策略(L-MobileNet)。将原标准卷积形式替换为深度可分离卷积形式,并将深度卷积层得到的特征图执行取反操作,通过深度卷积融合层传递至下一层;采用Leaky ReLU激活函数代替原ReLU激活函数来保留图像中更多的正负特征信息,并加入类残差结构避免梯度弥散现象。与6种方法进行对比,实验结果表明:L-MobileNet在数据集Cifar-10、Cifar-100(coarse)、Cifar-100(fine)和Dogs vs Cats上平均准确率和最高准确率都取得了最佳结果。
