This study was conducted to evaluate the diurnal effect on essential oil yield and composition of Ledum palustre L. grown in northern Inner Mongolia, China. Essential oil content and composition were determined and co...This study was conducted to evaluate the diurnal effect on essential oil yield and composition of Ledum palustre L. grown in northern Inner Mongolia, China. Essential oil content and composition were determined and compared as a function of different harvesting times viz. 7:00 AM, 11:00 AM, 3:00 PM, 7:00 PM, and 11:00 PM within a day. The essential oil obtained by hydrodistillation was investigated by gas chromatography-mass spectrometry (GC-MS). The yield of essential oil was varied from 1.21% to 1.62%;the maximum oil yield was obtained at 3:00 PM and the minimum at 7:00 PM. Similar to oil yield, qualitative difference in essential oil composition of L. palustre was observed. For the best essential oil yields, L. palustre should be harvested during 11:00 AM to 3:00 PM.展开更多
The tender leafstalks of Ledum palustre var.dilatatum were used as explants for the experiment.Uniform design for the most suitable media for shoots regeneration immediately at base of tender leafstalks,rooting and ge...The tender leafstalks of Ledum palustre var.dilatatum were used as explants for the experiment.Uniform design for the most suitable media for shoots regeneration immediately at base of tender leafstalks,rooting and germplasm conservation in vitro was screened.The results showed that N6+ZT 2.65 mg·L-1+IAA 0.05 mg·L-1 was fits for shoots regeneration,the frequency of shoots induction was higher than 92.5%;MS(modified)+IAA 0.1 mg·L-1+Kt 0.75 mg·L-1 for rooting,the rate of rooting was 98%;N-68+B9 2.5 mg·L-1+ Phloridzin 1.0 mg·L-1 for germplasm conservation in vitro for 46 months.Stems each with one node were cut from regenerated shoots and cultured for propagation,and a 90-fold proliferation rate was achieved within 30 days.The method of "deferring growth with dwarfing" was utilized for germplasm conservation in vitro at normal temperature.In vitro culture and germplasm conservation in vitro system of Ledum palustre var.dilatatum was established.展开更多
以杜香为原料,采用水、乙醇复合溶剂直接提取杜香挥发油。通过复合溶剂浸提法以单因素试验和正交试验研究提取温度、提取时间、料液比等因素对杜香挥发油提取的影响。结果表明,提取温度、料液比、溶剂配比是关键因子,提取时间、萃取次...以杜香为原料,采用水、乙醇复合溶剂直接提取杜香挥发油。通过复合溶剂浸提法以单因素试验和正交试验研究提取温度、提取时间、料液比等因素对杜香挥发油提取的影响。结果表明,提取温度、料液比、溶剂配比是关键因子,提取时间、萃取次数等影响较小。试验表明,在浸提温度20℃条件下,提取时间1.5 h,料液比1∶10,乙醇与水的配比为3∶2时,石油醚60 m L条件下,杜香油提取量最大为24.9 mg/g。展开更多
文摘This study was conducted to evaluate the diurnal effect on essential oil yield and composition of Ledum palustre L. grown in northern Inner Mongolia, China. Essential oil content and composition were determined and compared as a function of different harvesting times viz. 7:00 AM, 11:00 AM, 3:00 PM, 7:00 PM, and 11:00 PM within a day. The essential oil obtained by hydrodistillation was investigated by gas chromatography-mass spectrometry (GC-MS). The yield of essential oil was varied from 1.21% to 1.62%;the maximum oil yield was obtained at 3:00 PM and the minimum at 7:00 PM. Similar to oil yield, qualitative difference in essential oil composition of L. palustre was observed. For the best essential oil yields, L. palustre should be harvested during 11:00 AM to 3:00 PM.
文摘The tender leafstalks of Ledum palustre var.dilatatum were used as explants for the experiment.Uniform design for the most suitable media for shoots regeneration immediately at base of tender leafstalks,rooting and germplasm conservation in vitro was screened.The results showed that N6+ZT 2.65 mg·L-1+IAA 0.05 mg·L-1 was fits for shoots regeneration,the frequency of shoots induction was higher than 92.5%;MS(modified)+IAA 0.1 mg·L-1+Kt 0.75 mg·L-1 for rooting,the rate of rooting was 98%;N-68+B9 2.5 mg·L-1+ Phloridzin 1.0 mg·L-1 for germplasm conservation in vitro for 46 months.Stems each with one node were cut from regenerated shoots and cultured for propagation,and a 90-fold proliferation rate was achieved within 30 days.The method of "deferring growth with dwarfing" was utilized for germplasm conservation in vitro at normal temperature.In vitro culture and germplasm conservation in vitro system of Ledum palustre var.dilatatum was established.
文摘以杜香为原料,采用水、乙醇复合溶剂直接提取杜香挥发油。通过复合溶剂浸提法以单因素试验和正交试验研究提取温度、提取时间、料液比等因素对杜香挥发油提取的影响。结果表明,提取温度、料液比、溶剂配比是关键因子,提取时间、萃取次数等影响较小。试验表明,在浸提温度20℃条件下,提取时间1.5 h,料液比1∶10,乙醇与水的配比为3∶2时,石油醚60 m L条件下,杜香油提取量最大为24.9 mg/g。
