Endometrial Cancer Research Based on Gut Microbiomics and Metabolomics:An Analysis of Correlation and Differences

Endometrial cancer(EC)is a malignant tumour that occurs in the epithelial cells of the endometrium and represents one of the most common malignancies involving the female reproductive system,with endometrioid adenocarcinoma as the most common type.In recent years,with an increasingly aging society and the growing number of obese people,the incidence of EC is constantly rising,posing a serious threat to women’s health.Some studies have reported that the interruption of digestion and absorption caused by imbalance in intestinal microbiota may lead to conditions such as obesity,hypertension,diabetes,and hormone imbalance,which are all risk factors for EC.Meanwhile,intestinal bacteria produce a series of metabolites during colonization and reproduction,which can rapidly respond to changes in the microenvironment of the body.Changes in their types and quantities can serve as sensitive indicators of physiological and pathological changes in the body.Patients with EC often suffer from metabolic diseases,which can lead to metabolic disorders involving carbohydrates,fats,and amino acid in their bodies.

血清Leptin、Apelin水平与增生型糖尿病性视网膜病变患者玻璃体切割术后视力残疾的关系

目的探讨血清瘦素(Leptin)、爱帕琳肽(Apelin)水平与增生型糖尿病性视网膜病变(PDR)患者玻璃体切割术(PPV)后视力残疾的关系。方法选取接受PPV治疗的PDR患者198例(198眼),术后随访1年,根据是否发生视力残疾将患者分为视力残疾组48例、视力良好组150例。收集两组临床资料,术前用酶联免疫吸附试验检测血清Leptin、Apelin,多因素Logistic回归分析血清Leptin、Apelin水平对PDR患者PPV后视力残疾的影响,受试者工作特征(ROC)曲线分析血清Leptin、Apelin水平对PDR患者PPV后视力残疾的预测价值。结果与视力良好组比较,视力残疾组年龄大,PDR程度、术前虹膜新生血管比例、糖化血红蛋白水平及血清Leptin、Apelin水平高(P均<0.05)。多因素Logistic回归分析结果显示,重度PDR、术前虹膜新生血管、Leptin升高、Apelin升高为PDR患者PPV后视力残疾的独立危险因素(P均<0.05)。ROC曲线分析显示,血清Leptin、Apelin联合预测PDR患者PPV后视力残疾的曲线下面积为0.921,大于二者单独预测的曲线下面积(P均<0.05)。结论血清Leptin、Apelin水平升高是PDR患者PPV后视力残疾的危险因素,二者联合检测对PDR患者PPV后视力残疾的预测价值较高。

HE4、CEA和leptin表达对肺癌的诊断价值及其与病理分期、分化程度的关系

目的分析人附睾蛋白4(Human epididymis protein 4,HE4)、癌胚抗原(Carcinoembryonic antigen,CEA)和瘦素(leptin)表达水平对肺癌患者的诊断价值及其与患者病理分期、分化程度的关系。方法本研究纳入2022年1月~2023年12月在本院就诊的肺癌患者为研究组,共计120例,选取同期在本院体检的健康体检者为对照组以及经检查后为肺部良性疾病者为肺部良性疾病组,各60例。对比三组患者血清中HE4、CEA和leptin水平;对比不同分期以及不同细胞分化程度患者血清中HE4、CEA和leptin水平;分析血清中HE4、CEA和leptin水平与肺癌分期以及分化程度的相关性;采用受试者工作特征(Receiver operating characteristic,ROC)曲线分析血清中HE4、CEA、leptin单独或联合检测对肺癌的诊断价值。结果肺癌患者血清中HE4、leptin及CEA水平均显著高于肺部良性组和对照组(P<0.05);Ⅰ~Ⅱ期组患者血清中HE4、leptin及CEA水平均显著低于Ⅲ~Ⅳ期组(P<0.05);高分化组血清中HE4、leptin及CEA水平均显著高于中分化组以及低分化组(P<0.05);Spearman相关性分析表明血清中HE4、leptin和CEA水平与肺癌分期以及分化程度呈显著正相关(P<0.05);ROC曲线表明,HE4、CEA、leptin单独及联合检测诊断肺癌的曲线下面积分别为0.870、0.849、0.879以及0.958。结论肺癌患者的血清HE4、CEA和leptin水平均显著升高,且与肺癌的临床分期和细胞分化程度有一定的相关性,联合检测HE4、CEA和leptin指标对肺癌的诊断具有较高的价值,可以作为肺癌的筛查和早期诊断工具。

血清FGFR2、Leptin对特发性矮小症患儿的诊断价值及其水平与骨代谢指标的相关性

目的探讨血清成纤维生长因子受体2(FGFR2)、瘦素(Leptin)水平与特发性矮小症患儿骨代谢指标的相关性及其对疾病的诊断价值。方法选取2021年9月至2023年9月于该院就诊的138例特发性矮小症患儿为观察组,另选取同期于该院进行健康体检的141例健康儿童为对照组。采用酶联免疫吸附试验检测血清FGFR2、Leptin水平;采用全自动生化分析仪检测25-羟基维生素D[25-(OH)D]、Ⅰ型前胶原氨基酸前肽(PⅠNP)、骨钙素(OC)、骨特异性碱性磷酸酶(BAP)等骨代谢指标水平;采用Pearson相关分析特发性矮小症患儿血清FGFR2、Leptin水平与骨代谢指标水平的相关性;采用多因素Logistic回归分析影响特发性矮小症发病的因素;绘制受试者工作特征(ROC)曲线分析血清FGFR2、Leptin单独及联合检测对特发性矮小症的诊断价值。结果观察组体质量、身高及25-(OH)D、PⅠNP、OC、BAP、FGFR2、Leptin水平显著低于对照组,性发育状态Ⅱ~Ⅴ期患儿比例显著高于对照组,差异均有统计学意义(P<0.05)。Pearson相关性分析结果显示,特发性矮小症患儿血清FGFR2、Leptin水平与25-(OH)D、PⅠNP、OC、BAP水平均呈正相关(P<0.05)。多因素Logistic回归分析结果显示,FGFR2、Leptin水平升高是特发性矮小症发病的保护因素(P<0.05)。ROC曲线结果显示,血清FGFR2、Leptin单独及联合诊断特发性矮小症的曲线下面积分别为0.834、0.851、0.914。结论特发性矮小症患儿血清FGFR2、Leptin水平均较低,且二者均与骨代谢指标显著相关,推测其在诊断特发性矮小症中具有一定价值。

血清Leptin、CCL26在反复喘息患儿中的变化及与哮喘预测指数的相关性

目的分析血清瘦素(Leptin)、趋化因子配体26(chemokine ligand 26,CCL26)在反复喘息患儿中的变化,并探讨二者与哮喘预测指数(asthma predictive index,API)的相关性。方法选取反复喘息患儿178例作为观察组,并根据API分为API阳性组(n=92例)、API阴性组(n=86例),均进行规范管理。选取同期健康体检儿童89例为对照组。API阳性组、API阴性组在急性期、缓解期、对照组在入组时均检测血清Leptin、CCL26水平。分析血清Leptin、CCL26水平与API相关性及反复喘息患儿API阳性的影响因素。反复喘息患儿出院后均随访1年,对比随访期间是否再次发生喘息患儿血清Leptin、CCL26水平。结果观察组急性期、缓解期血清Leptin、CCL26水平均高于对照组(P<0.05);急性期、缓解期API阳性组血清Leptin、CCL26水平高于API阴性组(P<0.05);API阳性组、API阴性组急性期血清Leptin、CCL26水平高于缓解期(P<0.05);急性期、缓解期血清Leptin(r=0.480/0.401,P<0.001)、CCL26(r=0.481/0.512,P<0.001)水平与API均呈正相关;二元Logistic回归模型校正早产儿、变应性皮炎、烟草暴露史、母亲孕期主动或被动吸烟后,特应质、急性期血清Leptin及CCL26水平高表达仍会增加API阳性风险(P<0.05);建立Logistic回归模型:logit(P)=-7.005+1.020×特应质+1.098×急性期血清Leptin水平+1.058×急性期血清CCL26水平,经Hosmer-Lemeshow拟合优度检验显示,χ^(2)=4.249,DF=6,P=0.452,拟合度较好;178例反复喘息患儿随访1年,18例患儿再次发生喘息(10.11%),160例未发生喘息(89.89%),喘息患儿API阳性率高于未发生喘息患儿,急性期、缓解期血清Leptin、CCL26水平高于未发生喘息患儿(P<0.05)。结论反复喘息患儿血清Leptin、CCL26升高显著,且与API关系密切,是API阳性的独立危险因素。

Lean heart: Role of leptin in cardiac hypertrophy and metabolism

Leptin is an adipokine that has been linked with the cardiovascular complications resulting from obesity such as hypertension and heart disease. Obese patients have high levels of circulating leptin due to increased fat mass. Clinical and population studies have correlated high levels of circulating leptin with the development of cardiac hypertrophy in obesity. Leptin has also been demonstrated to increase the growth of cultured cardiomyocytes. However, several animal studies of obese leptin deficient mice have not supported a role for leptin in promoting cardiac hypertrophy so the role of leptin in this pathological process remains unclear. Leptin is also an important hormone in the regulation of cardiac metabolism where it supports oxidation of glucose and fatty acids. In addition, leptin plays a critical role in protecting the heart from excess lipid accumulation and the formation of toxic lipids in obesity a condition known as cardiac lipotoxicity. This paper focuses on the data supporting and refuting leptin's role in promoting cardiac hypertrophy as well as its important role in the regulation of cardiac metabolism and protection against cardiac lipotoxicity.

In ovo leptin administration affects hepatic lipid metabolism and microRNA expression in newly hatched broiler chickens

Background： A leptin-like immunoreactive substance has been found in chicken eggs and has been implicated in serving as a maternal signal to program offspring growth and metabolism. In the present study, we investigated the effects of in ovo leptin administration on hatch weight, serum and hepatic concentrations of metabolites and hormones, as well as on the expression of genes involved in hepatic lipid metabolism and the predicted microRNAs （miRNAs） targeting the affected genes. To this end we injected fertile eggs with either 0.5 μg of recombinant murine leptin or vehicle （PBS） before incubation.Results： Prenatally leptin-exposed chicks showed lower hatch weight, but higher liver weight relative to the body weight, compared to the control group. In ovo leptin treatment increased the hepatic content and serum concentration of leptin in newly hatched chickens. The hepatic contents of triglycerides （TG） and total cholesterol （Tch） were decreased, whereas the serum levels of TG, Tch and apolipoprotein B （ApoB） were increased. The hepatic mRNA expression of sterol regulator element binding protein 1 （SREBP-1c）, SREBP-2, hydroxy-3-methylglutaryl coenzyme A reductase （HMGCR） and cholesterol 7a-hydroxylase 1 （CYP7A1） was significantly up-regulated, as was the protein content of both SREBP-1 c and SREBP-2 in hepatic nuclear extracts of leptin-treated chickens. Moreover, out of 12 miRNAs targeting SREBP-1c and/or HMGCR, five were significantly up-regulated in liver of leptin-treated chicks, including gga-miR-200b and gga-miR-429, which target both SREBP-1c and HMGCR.Conclusions： These results suggest that leptin in ovo decreases hatch weight, and modifies hepatic leptin secretion and lipid metabolism in newly hatched broiler chickens, possibly via microRNA-mediated gene regulation.

Correlation of serum leptin levels with anthropometric and metabolic parameters and biochemical liver function in Chinese patients with chronic hepatitis C virus infection

AIM: To determine serum leptin levels and investigate their correlations with anthropometric and metabolic parameters and biochemical liver function in patients with chronic hepatitis C virus (HCV) infection and their potential clinical implications.METHODS: Forty-two chronic HCV-infected patients without anti-viral treatment were enrolled in this study, 30 patients had chronic hepatitis C, 10 had cirrhosis, and 2 had hepatocellular carcinoma (HCC). Thirty age- and sex-matched healthy individuals served as controls. Serum leptin levels were determined by ELISA. The biochemical liver function and serum lipids were determined at the same time. The height and body weight of patients and controls were measured, and body mass index (BMI) and body fat were calculated simultaneously. The correlations of serum leptin levels with anthropometric and metabolic parameters and biochemical liver function were assessed statistically.RESULTS: The mean of serum leptin levels in patients with chronic hepatitis C, HCV-associated cirrhosis, HCVassociated HCC and control groups was (6.13±3.94),(5.25±4.21), (4.17±0.28), and (3.59±3.44) ng/mL, respectively. The serum leptin level in patients with chronic hepatitis C was significantly higher than that in controls. The serum leptin levels between cirrhotic patients and controls and between male and female cirrhotic patients had no significant difference. Serum leptin levels were positively-correlated with body fat, BMI, and apolipoprotein B (Apo B) in patients with chronic HCV infection. The serum alanine aminotransferase (ALT) levels were closelycorrelated with BMI in patients with chronic hepatitis C. CONCLUSION: HCV infection interferes with fat and lipid metabolism in patients with chronic HCV infection and leptin may play a role in hepatosteatosis.

Disrupted leptin-fatty acid biosynthesis is an early manifestation of metabolic abnormalities in schizophrenia

BACKGROUND Insulin resistance(IR)and impaired energy expenditure(IEE)are irreparable metabolic comorbidities in schizophrenia.Although mechanism(s)underlying IR and IEE remains unclear,leptin and fatty acid signaling,which has profound influence on insulin secretion/sensitivity,glucose metabolism and energy expenditure,could be disrupted.However,no association of plasma leptin with erythrocyte membrane fatty acids,body mass index(BMI),and psychotic symptoms in the same cohort of untreated patients with first-episode psychosis(FEP)or medicated patients with chronic schizophrenia(CSZ)is presented before.These studies are crucial for deciphering the role of leptin and fatty acids in the development of IR and IEE in schizophrenia.AIM To determine the association between plasma leptin,erythrocyte membrane fatty acids,particularly,saturated fatty acids(SFAs),BMI and psychotic symptoms in patients with FEP and CSZ.METHODS In this study,twenty-two drug naive patients with FEP,twenty-one CSZ patients treated with atypical antipsychotic drugs,and fourteen healthy control(CNT)subjects were analyzed.Plasma leptin was measured using sandwich mode enzyme-linked immunosorbent assay.Erythrocyte membrane SFAs were measured using ultrathin capillary gas chromatography.BMI was calculated by using the formula:weight(kg)/height(m^(2)).Psychiatric symptoms were evaluated at baseline using brief psychiatric rating scale(BPRS),and positive and negative syndrome scale(PANSS).The total BPRS scores,positive and negative symptom scores(PANSS-PSS and PANSS-NSS,respectively)were recorded.Pearson correlation coefficient(r)analyses were performed to find the nature and strength of association between plasma leptin,PANSS scores,BMI and SFAs,particularly,palmitic acid(PA).RESULTS In patients with FEP,plasma leptin not BMI was significantly lower(P=0.034),whereas,erythrocyte membrane SFAs were significantly higher(P<0.005)compared to the CNT subjects.Further,plasma leptin showed negative correlation with erythrocyte membrane SFAs-PA(r=-0.4972,P=0.001),PANSS-PSS(r=-0.4034,P=0.028),and PANSS-NSS(r=-0.3487,P=0.048).However,erythrocyte membrane SFAs-PA showed positive correlation with PANSS-PSS(r=0.5844,P=0.0034)and PANSS-NSS(r=0.5380,P=0.008).In CSZ patients,plasma leptin,BMI,and erythrocyte membrane SFAs,all were significantly higher(P<0.05)compared to the CNT subjects.Plasma leptin showed positive correlation with BMI(r=0.312,P=0.032)but not with PANSS scores or erythrocyte membrane SFAs-PA.However,erythrocyte membrane SFAs-PA showed positive correlation with PANSS-NSS only(r=0.4729,P=0.031).Similar changes in the plasma leptin and erythrocyte membrane SFAs have also been reported in individuals at ultrahigh risk of developing psychosis;therefore,the above findings suggest that leptin-fatty acid biosynthesis could be disrupted before the onset of psychosis in schizophrenia.CONCLUSION Disrupted leptin-fatty acid biosynthesis/signaling could be an early manifestation of metabolic comorbidities in schizophrenia.Large-scale studies are warranted to validate the above findings.

Changes in Urinary Metabolomics of Female Kashin-Beck Disease Patients in Qinghai-Tibet Plateau,China

Kashin-Beck disease(KBD)is a chronic,endemic,degenerative osteoarthropathic condition that is predominantly found in China's Mainland.KBD is a progressive disease,and the signs and symptoms become more severe in adulthood owing to the continuous development of lesions in the joint cartilage[1].Although childhood KBD has largely been controlled,adult KBD is an important health concern in rural regions.Unfortunately,in the last century,many adult KBD patients still remain during serious epidemics.

Leptin influences estrogen metabolism and increases DNA adduct formation in breast cancer cells

Objective: The elevated incidence of obesity has been paralleled with higher risks of breast cancer. High adiposity increases leptin secretion from adipose tissue, which in turn increases cancer cell proliferation. The interplay between leptin and estrogen is one of the mechanisms through which leptin influences breast carcinogenesis. An unbalanced estrogen metabolism increases the formations of catechol estrogen quinones, DNA adducts, and cancer mutations. This study aims to investigate the effect of leptin on some estrogen metabolic enzymes and DNA adduction in breast cancer cells.Methods: High performance liquid chromatography(HPLC) was performed to analyze the DNA adducts 4-OHE1[E2]-1-N3 adenine and 4-OHE1[E2]-1-N7 guanine. Reporter gene assay, real time reverse transcription polymerase chain reaction(real time RT-PCR), and Western blot were used to assess the expression of estrogen metabolizing genes and enzymes: Cytochrome P-4501B1(CYP1B1), Nicotinamide adenine dinucleotide phosphate-quinone oxidoreductase1(NQO1), and Catechol-O-methyl transferase(COMT).Results: Leptin significantly increased the DNA adducts 4-OHE1[E2]-1-N3 adenine and 4-OHE1[E2]-1-N7 guanine.Furthermore, leptin significantly upregulated CYP1B1 promoter activity and protein expression. The luciferase promoter activities of NQO1 and m RNA levels were significantly reduced. Moreover, leptin greatly reduced the reporter activities of the COMT-P1 and COMT-P2 promoters and diminished the protein expression of COMT.Conclusions: Leptin increases DNA adduct levels in breast cancer cells partly by affecting key genes and enzymes involved in estrogen metabolism. Thus, increased focus should be directed toward leptin and its effects on the estrogen metabolic pathway as an effective approach against breast cancer.

Leptin循环水平与结直肠腺瘤及结直肠癌的关联性:一项病例对照和孟德尔随机化研究

目的探究Leptin循环水平与结直肠腺瘤及结直肠癌发生风险的关联,为结直肠腺瘤及结直肠癌的防治提供依据。方法以浙江大学医学院附属第一医院、浙江省肿瘤医院、浙江省诸暨市人民医院及杭州市临安区第一人民医院为研究现场,共收集结直肠腺瘤患者497例,结直肠癌患者955例,健康对照911例。通过问卷收集研究对象的基本信息,并采集血液样本和提取DNA,选取Leptin的工具变量并进行基因分型检测。采用Logistic回归模型并进行分层分析,评估血清Leptin水平与结直肠腺瘤,结直肠癌以及结直肠腺瘤进展为结直肠癌的关联。进一步将遗传风险评分(GRS)和单核苷酸多态性(SNPs)作为工具变量,采用两阶段最小二乘法和逆方差加权法进行单样本和两样本孟德尔随机化分析,来评估Leptin循环水平与结直肠腺瘤,结直肠癌及结直肠腺瘤进展为结直肠癌风险之间潜在的因果关联。结果病例对照研究结果显示,与最低四分数的Leptin水平相比,高水平的Leptin与结直肠腺瘤呈正相关(OR=1.63,95%CI:1.16~2.30,P=0.005),高水平的Leptin与结直肠癌呈负相关(OR=0.53,95%CI:0.38~0.74,P<0.001),高水平的Leptin也与结直肠腺瘤患者进展为结直肠癌的风险呈负相关(OR=0.44,95%CI:0.29~0.66,P<0.001)。而孟德尔随机化分析结果显示,Leptin的GRS无论是否加权,均与结直肠腺瘤,结直肠癌以及结直肠腺瘤进展为结直肠癌的风险无统计学关联。两样本孟德尔随机化研究也未发现Leptin与结直肠癌风险之间的关联(P>0.05)。结论病例对照研究结果提示Leptin可能与结直肠腺瘤,结直肠癌及结直肠腺瘤进展为结直肠癌的风险存在关联,但孟德尔随机化研究尚不支持Leptin与结直肠腺瘤,结直肠癌及结直肠腺瘤进展为结直肠癌风险之间存在因果关联。

Metabolomic Profiling of Mice Exposed toα-amanitin Using Ultra-performance Liquid Chromatography Quadrupole Time-of-flight Tandem Mass Spectrometry

Numerous instances of poisoning caused by the ingestion of mushrooms are reported annually worldwide,with most mushroom poisoning deaths caused by the ingestion of toxic amanita fungi.Amatoxin is the main toxic component of amanita,andα-amanitin is one of the most potent toxins present in amatoxin[1].

Metabotropic glutamate receptor 1 alpha:a unique receptor variant with variable implications for Alzheimer’s disease pathogenesis

Alzheimer’s disease (AD) is the leading neurodegenerative disorder globally.Despite this,there is minimal effective therapeutics proven to reduce or prevent the progression of this disease.Glutamate is the main excitatory neurotransmitter within the central nervous system and plays a crucial role in neuronal and synaptic functions.

NPDR和PDR患者角膜生物力学参数及血清Leptin和ecSOD含量比较

目的:探究非增殖型糖尿病视网膜病变(NPDR)和增殖型糖尿病视网膜病变(PDR)患者角膜生物力学参数及血清瘦素(Leptin)、细胞外超氧化物歧化酶(ecSOD)含量差异性。方法:前瞻性研究。选择2020-05/2022-05我院收治的2型糖尿病(T2DM)合并糖尿病视网膜病变(DR)患者118例,按照病变程度分为NPDR组57例、PDR组61例,另选54例T2DM无视网膜病变患者和52例体检健康人群分别作为NDR组和对照组,所有参与研究者均选左眼入组。比较各组角膜生物力学参数[角膜中央厚度(CCT),眼内压(IOP),等效球镜度(SE),第一次压平时间(A1T),第一次压平长度(A1L),最大形变幅度(DA)]及血清Leptin、ecSOD含量差异,多因素Logistic回归分析影响PDR发生的高危因素。结果:PDR组、NPDR组患者CCT、IOP、A1T高于对照组和NDR组,DA低于对照组和NDR组(均P<0.05),且PDR组CCT、IOP、A1T高于NPDR组(均P<0.05);PDR组、NPDR组、NDR组血清Leptin、ecSOD水平均高于对照组(均P<0.05);NPDR组和PDR组DM病程、CCT、IOP、A1T及血清Leptin、ecSOD含量有差异(均P<0.05);多因素Logistic回归分析显示,DM病程、CCT、IOP、A1T及血清Leptin、ecSOD含量是影响PDR发生的因素,DA是影响PDR发生的保护因素(均P<0.05)。结论:PDR患者CCT、IOP及血清Leptin、ecSOD含量较NPDR患者明显增加,DA明显降低,且CCT、IOP、A1T、血清Leptin、ecSOD含量是影响PDR发生的高危因素,DA是影响PDR发生的保护因素。

Relationship between Serum Leptin and Adiponectin Levels in Obese Nigerians with Type 2 Diabetes Subjects

Background: Obesity is rising globally, independent of ethnicity, race and age, and is associated with increased risk of cardiovascular mortality and morbidity especially in persons living with diabetes. The effect of adipokines such as leptin, resistin and adiponectin which are secreted by adipose tissue factors has been linked to the increased risk of the cardiovascular mortality and morbidity. Aim: This study aims to assess the levels of serum leptin and serum adiponectin in obese type 2 diabetes subjects, and their relationship with cardio-metabolic component (using lipid profile). Method: This is a cross-sectional comparative hospital-based study in which one hundred and fifty participants grouped into 60 obese, 60 non-obese none diabetic and 30 non-obese non-diabetic adults with similar age from the Endocrinology outpatient’s clinic, General outpatient department (GOPD) and staff clinic of Obafemi Awolowo University Teaching Hospitals Complex (OAUTHC). Anthropometric parameters and other relevant clinical details of all subjects were obtained. Fasting venous blood samples were taken from all subjects for the determination of fasting plasma glucose (FPG), fasting lipid profile, glycosylated haemoglobin levels (HbA1C), fasting serum insulin, leptin, and adiponectin. Data was analysed using the Statistical Package of Social Sciences (SPSS) version 23.0. Results: Twenty-eight (46.7%)) subjects were males while 32 subjects (53.3%) were females (in obese groups). There was no statistical difference between these two groups for both sexes (p = 1.000). The age range for all subjects was 34 to 64 years with their mean age being 52 ± 7.3 years, and for type 2 subjects and the obese non-diabetic 50.7 ± 7.3 years respectively. The mean body mass index of the non-obese and non-diabetic was 23.7 ± 4 Kg/m2 while the anthropometric measurements of the obese two groups were similar. The lipid profile, serum leptin and adiponectin in both the obese groups showed no difference. The relationship between components of obesity and serum leptin level in all subjects showed that serum leptin levels had significant positive correlation with BMI, WC, and serum insulin level. The correlation between adiponectin and lipid profile showed a positive correlation between serum adiponectin values and High-density lipoprotein (HDL) in obese diabetic (p = 0.02) but not in the non-diabetic group. Conclusion: In this study, there was no correlation in the serum leptin levels with the anthropometric parameters of obesity studied. For the components of the lipid profile, Triglycerides and low-density lipoprotein cholesterol (LDL) correlated with serum adiponectin and HDL showed a positive correlation with adiponectin. It is concluded that the effects of both serum adiponectin and serum leptin are driven by obesity rather than the glycaemic status of the obese subjects.

血清Leptin、MCP-1预测急性带状疱疹患者后遗神经痛的临床价值

目的探讨血清瘦素(Leptin)、单核细胞趋化蛋白-1(MCP-1)水平预测急性带状疱疹患者发生后遗神经痛(PHN)的临床价值。方法前瞻性选择2020年1月至2022年1月经安阳市人民医院确诊的168例急性带状疱疹患者作为研究对象,治疗后随访3个月,根据是否出现PHN分为PHN组78例,无PHN组90例。同时选择同期在我院体检的96例健康人作为健康组。检测并比较所有受检者的血清Leptin、MCP-1水平;采用Pearson相关法分析血清Leptin、MCP-1与数字评分法(NRS)评分的相关性,采用受试者工作特征曲线(ROC)及曲线下面积(AUC)评估血清Leptin、MCP-1水平对急性带状疱疹患者发生PHN的预测价值,同时采用多因素Logistic回归分析探究影响急性带状疱疹患者发生PHN的危险因素。结果PHN组患者的血清Leptin、MCP-1水平明显低于无PHN组和健康组,无PHN组明显低于健康组,差异均有统计学意义(P<0.05);经Pearson相关分析结果显示,急性带状疱疹患者血清Leptin、MCP-1与NRS评分均呈负相关(r=-0.406、-0.452,P<0.001);经ROC分析结果显示,血清Leptin预测急性带状疱疹患者发生PHN的AUC为0.769,截断值为16.8μg/L,敏感度、特异度分别为92.31%、50.00%;血清MCP-1预测急性带状疱疹患者发生PHN的AUC为0.832,截断值为101.4 pg/μL,敏感度、特异度分别为92.31%、67.78%;血清Leptin联合MCP-1预测急性带状疱疹患者发生PHN的AUC为0.921,敏感度、特异度分别为85.90%、85.56%;经多因素Logistic回归分析结果显示,血清Leptin、MCP-1、NRS评分均为影响急性带状疱疹患者发生PHN的危险因素(P<0.05)。结论急性带状疱疹患者血清Leptin、MCP-1水平均降低,且与NRS评分均呈负相关,有望作为预测急性带状疱疹患者发生PHN的有效生物学标志物。

ISS评分联合血清Leptin、CRP水平检测在胸腹部创伤患者预后预测中的应用价值

目的:探讨创伤严重度评分(ISS)联合血清瘦素(Leptin)、C反应蛋白(CRP)水平检测在胸腹部创伤患者预后预测中的应用价值。方法:回顾性分析2020年5月至2021年5月该院收治的106例胸腹部创伤患者的临床资料。根据预后情况,将其分为生存组(90例)及死亡组(16例)。比较两组血清CRP、Leptin水平及ISS评分;采用受试者工作特征曲线(ROC)分析ISS评分联合血清Leptin、CRP水平在胸腹部创伤患者预后预测中的应用价值。结果:死亡组血清ISS评分及血清Leptin、CRP水平均高于生存组,差异有统计学意义(P<0.05);ROC曲线分析结果显示,血清ISS评分联合血清Leptin、CRP水平检测预测胸腹部创伤患者预后的曲线下面积为0.869,分别高于三者单项检测的0.742、0.748、0.726,差异有统计学意义(P<0.05)。结论:ISS评分联合血清Leptin、CRP水平检测在胸腹部创伤患者预后预测中具有较高的应用价值。

老年血液透析病人衰弱与血清瘦素及25-羟维生素D的相关性研究

目的探究老年血液透析病人血清瘦素及25-羟维生素D[25(OH)D]水平,并分析其与透析病人衰弱的相关性。方法选取2021年4月至2023年4月我院收治的150例老年血液透析病人,依据Fried表型评价病人的衰弱情况,分为非衰弱组(51例),衰弱前期组(74例)和衰弱组(25例)。比较3组一般资料、血清瘦素、25(OH)D及心功能。采用多因素Logistic回归分析老年血液透析病人衰弱的影响因素。结果3组年龄、糖尿病比例、透析龄、左室舒张末期内径,以及白蛋白、镁、IL-6、瘦素、25(OH)D水平比较,差异均有统计学意义(P<0.01)。多因素有序Logistic回归分析显示,年龄、透析龄、糖尿病、镁、IL-6、白蛋白、瘦素、25(OH)D、左室舒张末期内径是老年血液透析病人衰弱的独立影响因素(P<0.05)。结论针对白蛋白水平低、瘦素水平高、25(OH)D水平低的老年血液透析病人采取积极干预措施,有利于延缓衰弱的进展。

癫痫患儿血清serotonin、leptin的水平及临床意义

目的 探讨癫痫患儿血清中血清素(serotonin)、瘦素(leptin)的水平及其临床意义。方法 选取2019年4月至2021年4月咸宁市中心医院收治的102例癫痫患儿作为研究对象,随后根据癫痫发作频率分为频发组(A组,35例)、持续状态组(B组,27例)、非频发或持续状态组(C组,40例)。另选取同期在该院体检的102例健康儿童作为对照组。分别采用酶联免疫吸附法、放射免疫法检测儿童血清serotonin、leptin水平。采用Pearson相关分析探讨血清serotonin、leptin水平与C反应蛋白(CRP)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)的关系,同时采用ROC曲线评价血清serotonin、leptin评估癫痫的临床价值。结果 癫痫各组及对照组儿童的血清serotonin、leptin的水平差异均有统计学意义(F值分别为76.710、79.640,P<0.05);进一步比较发现,serotonin水平:对照组>C组>B组>A组;leptin水平:A组>B组>C组>对照组,差异均有统计学意义(P<0.05)。癫痫各组及对照组儿童血清CRP、IL-2、TNF-α表达水平差异均有统计学意义(F值分别为813.920、560.602、507.530,P<0.05);进一步比较发现,A组>B组>C组>对照组,差异均有统计学意义(P<0.05)。相关性分析显示,癫痫患儿血清serotonin与CRP、IL-2、TNF-α均呈负相关(r值分别为-0.534、-0.528、-0.476,P<0.05),血清leptin与CRP、IL-2、TNF-α均呈正相关(r值分别为0.425、0.433、0.457,P<0.05)。血清serotonin、leptin评估癫痫的曲线下面积(AUC)及其95%CI分别为0.819(0.759~0.879)、0.835(0.765~0.897),二者联合检测的AUC(95%CI)为0.887(0.816~0.966)。结论 癫痫患儿血清中serotonin表达水平降低,leptin水平升高,且与癫痫发作频率及预后密切相关。Serotonin和leptin水平在辅助诊断癫痫上具有一定的价值。