目的通过研究原发性高血压(EH)患者的血清Lp(a)、Lp-PLA2水平与血压变异性(BPV)的特征,进一步探讨EH患者血清中Lp(a)和Lp-PLA2水平与BPV之间的关系。方法选取2022年9月至2023年9月于内蒙古自治区人民医院门诊及住院诊疗的EH患者240例。...目的通过研究原发性高血压(EH)患者的血清Lp(a)、Lp-PLA2水平与血压变异性(BPV)的特征,进一步探讨EH患者血清中Lp(a)和Lp-PLA2水平与BPV之间的关系。方法选取2022年9月至2023年9月于内蒙古自治区人民医院门诊及住院诊疗的EH患者240例。将患者分为A组:Lp(a)、Lp-PLA2均正常,115例;B组:仅Lp(a)升高,48例;C组:仅Lp-PLA2升高,42例;D组:Lp(a)、Lp-PLA2均升高,35例。探索Lp(a)和Lp-PLA2水平与血压变异性之间的相关性。结果B组各时段变异系数(CV)均高于A组(P<0.05);C组24 h SBPCV、24 h DBP CV、nSBP CV、dDBP CV、dSBP CV高于A组(P<0.05);D组各时段CV均高于A组(P<0.05)。Lp(a)、Lp-PLA2与BPV呈正相关(rs>0,P<0.05)。B组、C组以及D组的Lp(a)、Lp-PLA2和24 h SBP CV、24 h DBP CV间均呈显著正相关关系(rs>0,P<0.05)。结论EH患者血清Lp(a)、Lp-PLA2水平与BPV呈正相关,且血清Lp(a)、Lp-PLA2均升高的患者BPV也升高。展开更多
Objective:To analyze scrum leptin levels in patients with malaria falciparum and compare them with healthy controls and correlate with development and outcome of malaria infection.Methods:Sixty cases of malaria falcip...Objective:To analyze scrum leptin levels in patients with malaria falciparum and compare them with healthy controls and correlate with development and outcome of malaria infection.Methods:Sixty cases of malaria falciparum were included in this study as patients.Thirty healthy individuals of comparable age,racial and body mass index were taken as controls.All patients were diagnosed by clinical picture and the presence of malaria parasites in blood film.Estimation of liver function test,kidney function test,complete blood count,fasting blood sugar,fasting serum insulin,pro-inflammatory cytokine tumor necrosis factor alpha(TNFα)and interleukin 1(IL1),estimation of morning serum leptin and calculation of body mass index(kg/m^2)were done in both groups on the day of admission,on discharge and 7 d after discharge.Results:At admission,leptin levels were significantly higher in patients group than in control while lasting serum insulin levels were not significantly different between the two groups.There were significant increases as regard to TNFαand IL1 in malaria patients.Significant differences were observed between the control and the patient group for leptin,TNFαand IL1 at the time of admission and discharge.After discharge for 7 d.a significant decline in scrum leptin levels,TNFαand IL1 in the patients group was observed as compared with time of admission and time of discharge,a positive correlation between serum leptin levels and TNFαand IL1.Conclusions:Leptin hormone level might play an important role in development and outcome of malaria infection.展开更多
Adipocytokines, such as adiponectin and leptin, are expressed by adipocytes and are known as anti-metabolic syndrome factors. They are also thought to mediate calcification in mineralized tissue. We investigated the e...Adipocytokines, such as adiponectin and leptin, are expressed by adipocytes and are known as anti-metabolic syndrome factors. They are also thought to mediate calcification in mineralized tissue. We investigated the effects of adiponectin and leptin on the kinetics of human pulp cells using ELISA and western blot. After gaining informed consent, we obtained human pulp cells from three patients. After cultivation in Dulbecco’s Modified Eagle Medium (DMEM) without serum, cells from the 4th to 6th passages were incubated with various concentrations of adiponectin (1, 10, or 100 ng/mL) in DMEM or leptin (0.1, 1, or 10 ng/mL) in DMEM for 24 h. We confirmed that human pulp cells expressed adiponectin receptor 1 and leptin receptor. Although the proliferation index of these cells as measured by 5-bromo-2'-deoxyuridine (BrdU) incorporation increased in the presence of adiponectin in a dose-de<span>pendent manner, pulp cells stimulated with leptin showed no significant changes in BrdU incorporation. Alkaline phosphatase activity showed no significant changes after stimulation with either adipocytokine. Adiponectin induced expression of BMP-2 and osteopontin more strongly than did leptin. In particular, expression of BMP-2 increased in the presence of adiponectin i</span><span>n a dose-dependent manner. In contrast, the expression of dentin sialopr</span><span>otein increased after stimulation with leptin. The expression of Runx2 was not observed from the cultured pulp cells stimulated with both molecules.</span><span> </span><span>These results indicate that adiponectin and leptin contribute to growth and differentiation of human pulp cells and may consequently affect the formation of secondary dentin or reparative dentin in the dentin-pulp complex.</span>展开更多
文摘目的通过研究原发性高血压(EH)患者的血清Lp(a)、Lp-PLA2水平与血压变异性(BPV)的特征,进一步探讨EH患者血清中Lp(a)和Lp-PLA2水平与BPV之间的关系。方法选取2022年9月至2023年9月于内蒙古自治区人民医院门诊及住院诊疗的EH患者240例。将患者分为A组:Lp(a)、Lp-PLA2均正常,115例;B组:仅Lp(a)升高,48例;C组:仅Lp-PLA2升高,42例;D组:Lp(a)、Lp-PLA2均升高,35例。探索Lp(a)和Lp-PLA2水平与血压变异性之间的相关性。结果B组各时段变异系数(CV)均高于A组(P<0.05);C组24 h SBPCV、24 h DBP CV、nSBP CV、dDBP CV、dSBP CV高于A组(P<0.05);D组各时段CV均高于A组(P<0.05)。Lp(a)、Lp-PLA2与BPV呈正相关(rs>0,P<0.05)。B组、C组以及D组的Lp(a)、Lp-PLA2和24 h SBP CV、24 h DBP CV间均呈显著正相关关系(rs>0,P<0.05)。结论EH患者血清Lp(a)、Lp-PLA2水平与BPV呈正相关,且血清Lp(a)、Lp-PLA2均升高的患者BPV也升高。
基金supported by College of Health Sciences(Grant No,HS-10-01)
文摘Objective:To analyze scrum leptin levels in patients with malaria falciparum and compare them with healthy controls and correlate with development and outcome of malaria infection.Methods:Sixty cases of malaria falciparum were included in this study as patients.Thirty healthy individuals of comparable age,racial and body mass index were taken as controls.All patients were diagnosed by clinical picture and the presence of malaria parasites in blood film.Estimation of liver function test,kidney function test,complete blood count,fasting blood sugar,fasting serum insulin,pro-inflammatory cytokine tumor necrosis factor alpha(TNFα)and interleukin 1(IL1),estimation of morning serum leptin and calculation of body mass index(kg/m^2)were done in both groups on the day of admission,on discharge and 7 d after discharge.Results:At admission,leptin levels were significantly higher in patients group than in control while lasting serum insulin levels were not significantly different between the two groups.There were significant increases as regard to TNFαand IL1 in malaria patients.Significant differences were observed between the control and the patient group for leptin,TNFαand IL1 at the time of admission and discharge.After discharge for 7 d.a significant decline in scrum leptin levels,TNFαand IL1 in the patients group was observed as compared with time of admission and time of discharge,a positive correlation between serum leptin levels and TNFαand IL1.Conclusions:Leptin hormone level might play an important role in development and outcome of malaria infection.
文摘Adipocytokines, such as adiponectin and leptin, are expressed by adipocytes and are known as anti-metabolic syndrome factors. They are also thought to mediate calcification in mineralized tissue. We investigated the effects of adiponectin and leptin on the kinetics of human pulp cells using ELISA and western blot. After gaining informed consent, we obtained human pulp cells from three patients. After cultivation in Dulbecco’s Modified Eagle Medium (DMEM) without serum, cells from the 4th to 6th passages were incubated with various concentrations of adiponectin (1, 10, or 100 ng/mL) in DMEM or leptin (0.1, 1, or 10 ng/mL) in DMEM for 24 h. We confirmed that human pulp cells expressed adiponectin receptor 1 and leptin receptor. Although the proliferation index of these cells as measured by 5-bromo-2'-deoxyuridine (BrdU) incorporation increased in the presence of adiponectin in a dose-de<span>pendent manner, pulp cells stimulated with leptin showed no significant changes in BrdU incorporation. Alkaline phosphatase activity showed no significant changes after stimulation with either adipocytokine. Adiponectin induced expression of BMP-2 and osteopontin more strongly than did leptin. In particular, expression of BMP-2 increased in the presence of adiponectin i</span><span>n a dose-dependent manner. In contrast, the expression of dentin sialopr</span><span>otein increased after stimulation with leptin. The expression of Runx2 was not observed from the cultured pulp cells stimulated with both molecules.</span><span> </span><span>These results indicate that adiponectin and leptin contribute to growth and differentiation of human pulp cells and may consequently affect the formation of secondary dentin or reparative dentin in the dentin-pulp complex.</span>
