Lespedeza bicolor Turcz is a native shrub in the temperate region of China. Thirteen provenances of L. bicolor were investigated in our study. Morphological diversity among and within provenances were analyzed based o...Lespedeza bicolor Turcz is a native shrub in the temperate region of China. Thirteen provenances of L. bicolor were investigated in our study. Morphological diversity among and within provenances were analyzed based on 19 phenotypic traits, including seven vegetative growth characteristics, nine floral traits and three pod characteristics. The coefficient of variation （CV） ranged from 6.4% to 65.4% and the phenotypic differentiation coefficient from 43.3% to 97.3%. Analyses of variance showed that there were extremely significant variations among and within provenances in pod length. Differences in plant height, inter-nodal length, number of branches, leaf width, leaf length and leaf area were not remarkable among provenance, but distinct within provenances. Other traits showed evidence of contrasts. Variation among provenances was the main part of phenotypic variation. Correlation analysis indicated that there were highly significant positive correlations between plant height and other vegetative growth characteristics. Floral traits were not associated with vegetative growth characteristics. The 13 provenances of L. bicolor investigated can be classified into three groups according to a UPGMA cluster analysis.展开更多
In order to find the optimal bacteriostatic agents and selectors and their concentrations in genetic transformation of Lespedeza bicolor with BADH as a marker gene, an resistance experiment was carried out with four c...In order to find the optimal bacteriostatic agents and selectors and their concentrations in genetic transformation of Lespedeza bicolor with BADH as a marker gene, an resistance experiment was carried out with four chemicals, NaCl, betaine aldehyde, PEG and cefotaxime （Cef） added into the media, Different concentrations and combinations of different chemicals were added into the media in the stage of adventitious bud regeneration of cotyledonary nodes, Shoot segment multiplication, and rooting. The results showed that NaCI and betaine aldehyde could inhibit tissue differentiation of L. bicolor, and could thus be used as selectors. Furthermore, 0.8 g/L NaCI could completely inhibit the cotytedonary node differentiation and shoot segment multiplication, and 0.5 g/L NaCI was optimal for inhibition of rooting. It was also found that with the concentration increase of betaine aldehyde in media, adventitious buds regenerated from cotyledonary nodes decreased, and 1.5 g/L was confirmed to be the optimum concentration. Moreover, the mixture of 0.7 g/L NaCI and 0.3 g/L betaine aldehyde significantly inhibited shoot segment multiplication and rooting, so this mixture could be used asa selector. The Cef ex- periment showed that 200-300 mg/L C, ef in media did not inhibit significantly the regeneration of adventitious buds from cotyledonary nodes, and 100 mg/L Cef had little effect on the shoot segment multiplication and rooting.展开更多
Ultra-drying (in a desiccator with silica gel) and accelerated aging (50 oC, 30 d) of Lespedeza bicolor seeds from the arid and semiarid areas of northwest China were studied. The results showed that in the moisture c...Ultra-drying (in a desiccator with silica gel) and accelerated aging (50 oC, 30 d) of Lespedeza bicolor seeds from the arid and semiarid areas of northwest China were studied. The results showed that in the moisture content of seed ( 43?70 g/kg), germination percentage and vigor indices had no significant differences; ultra-dried seeds had a stronger anti-aging ability than those with natural drying, and the best MC for the storage was 43 g/kg. Therefore, it was feasible to store L. bicolor seeds with ultra-dried moisture content.展开更多
卫星搭载二色胡枝子(L esp ed eza bicolor)干种子,返地后对其出苗率、株高、生长速度和生育期等性状进行观察。结果表明,卫星搭载当代,种子出苗率、生长速度和株高显著降低;过氧化物同工酶检测显示谱带发生变化,表明卫星搭载对种子的...卫星搭载二色胡枝子(L esp ed eza bicolor)干种子,返地后对其出苗率、株高、生长速度和生育期等性状进行观察。结果表明,卫星搭载当代,种子出苗率、生长速度和株高显著降低;过氧化物同工酶检测显示谱带发生变化,表明卫星搭载对种子的生理生化活动产生明显的影响。展开更多
文摘Lespedeza bicolor Turcz is a native shrub in the temperate region of China. Thirteen provenances of L. bicolor were investigated in our study. Morphological diversity among and within provenances were analyzed based on 19 phenotypic traits, including seven vegetative growth characteristics, nine floral traits and three pod characteristics. The coefficient of variation （CV） ranged from 6.4% to 65.4% and the phenotypic differentiation coefficient from 43.3% to 97.3%. Analyses of variance showed that there were extremely significant variations among and within provenances in pod length. Differences in plant height, inter-nodal length, number of branches, leaf width, leaf length and leaf area were not remarkable among provenance, but distinct within provenances. Other traits showed evidence of contrasts. Variation among provenances was the main part of phenotypic variation. Correlation analysis indicated that there were highly significant positive correlations between plant height and other vegetative growth characteristics. Floral traits were not associated with vegetative growth characteristics. The 13 provenances of L. bicolor investigated can be classified into three groups according to a UPGMA cluster analysis.
基金Supported by The Agricultural Science and Technology Project of Beijing(PXM2016-014207-000053)~~
文摘In order to find the optimal bacteriostatic agents and selectors and their concentrations in genetic transformation of Lespedeza bicolor with BADH as a marker gene, an resistance experiment was carried out with four chemicals, NaCl, betaine aldehyde, PEG and cefotaxime （Cef） added into the media, Different concentrations and combinations of different chemicals were added into the media in the stage of adventitious bud regeneration of cotyledonary nodes, Shoot segment multiplication, and rooting. The results showed that NaCI and betaine aldehyde could inhibit tissue differentiation of L. bicolor, and could thus be used as selectors. Furthermore, 0.8 g/L NaCI could completely inhibit the cotytedonary node differentiation and shoot segment multiplication, and 0.5 g/L NaCI was optimal for inhibition of rooting. It was also found that with the concentration increase of betaine aldehyde in media, adventitious buds regenerated from cotyledonary nodes decreased, and 1.5 g/L was confirmed to be the optimum concentration. Moreover, the mixture of 0.7 g/L NaCI and 0.3 g/L betaine aldehyde significantly inhibited shoot segment multiplication and rooting, so this mixture could be used asa selector. The Cef ex- periment showed that 200-300 mg/L C, ef in media did not inhibit significantly the regeneration of adventitious buds from cotyledonary nodes, and 100 mg/L Cef had little effect on the shoot segment multiplication and rooting.
基金supported by the National Key Project of Scientific and Technical Supporting Programs of China under the Grant No. 2006BAD09B06 and 2006BAD03A0308the "948" program of Ministry of Water Resources of P. R. China under Grant No. 200207
文摘Ultra-drying (in a desiccator with silica gel) and accelerated aging (50 oC, 30 d) of Lespedeza bicolor seeds from the arid and semiarid areas of northwest China were studied. The results showed that in the moisture content of seed ( 43?70 g/kg), germination percentage and vigor indices had no significant differences; ultra-dried seeds had a stronger anti-aging ability than those with natural drying, and the best MC for the storage was 43 g/kg. Therefore, it was feasible to store L. bicolor seeds with ultra-dried moisture content.
