Benzoxaborole,a five-membered oxaborole ring fused with a phenyl ring,has demonstrated potent pharmacological activity.In order to explore their potential applications in agriculture,five-membered and six-membered ben...Benzoxaborole,a five-membered oxaborole ring fused with a phenyl ring,has demonstrated potent pharmacological activity.In order to explore their potential applications in agriculture,five-membered and six-membered benzoxaborole derivatives were synthesized and evaluated for their fungicidal activity against six common plant pathogenic fungi in vitro.The bioassay results showed that most of the target compounds exhibited significant fungicidal activity at concentrations below 50μg/mL,particularly the highlighted compounds 4b and 4e,which demonstrated impressive fungicidal activity superior to those of the positive controls.Molecular docking was also performed to confirm the practical value of the active compound as a potential inhibitor of Leucyl-tRNA Synthetase(LeuRS).This study indicates that the designed benzoxaborole derivatives could serve as template molecules for the development of novel fungicides.展开更多
The chemical modification of the sulfhydryl groups of E. coli Leucyl--tRNA synthetase(LeuRS) by DTNB, NEM and IAA resulted in a time-dependent loss of both amino-acid acti-vation and aminoacylation activities in paral...The chemical modification of the sulfhydryl groups of E. coli Leucyl--tRNA synthetase(LeuRS) by DTNB, NEM and IAA resulted in a time-dependent loss of both amino-acid acti-vation and aminoacylation activities in parallel. The second-order reaction constants of DTNB,NEM and IAA were 1700, 150 and 0.46 mol/L^(-1) min^(-1) respectively. Chemical stoichiometryshowed that only one sulfhydryl group of LeuRS was essential for both activities. Substratesleucine and Leu-AMP protected the active sulfhydryl group from modification, suggestingthat the modified sulfhydryl group is located in or near the active site region responsiblefor amino-acid activation. [^(14)C]NEM--labeled LeuRS was subjected to tryptic digestion, andpeptides were separated and sequenced. 179 Cys~*-Asp-Thr-Leu182 was identified as the major[^(14)C]NEM-labeled site in LeuRS. This result is consistent with the previous observationthat the region for Leu--AMP formation was located at the N--terminal part of LeuRS.展开更多
基金the financial support for this research from the National Natural Science Foundation of China(22177051,32061143045)the Fundamental Research Funds for the Central Universities(KYCYXT2022010)+1 种基金Sichuan Key Research and Development Program(22ZDYF0186,2021YFN0134)the College Student Research Training Program(202110307002T).
文摘Benzoxaborole,a five-membered oxaborole ring fused with a phenyl ring,has demonstrated potent pharmacological activity.In order to explore their potential applications in agriculture,five-membered and six-membered benzoxaborole derivatives were synthesized and evaluated for their fungicidal activity against six common plant pathogenic fungi in vitro.The bioassay results showed that most of the target compounds exhibited significant fungicidal activity at concentrations below 50μg/mL,particularly the highlighted compounds 4b and 4e,which demonstrated impressive fungicidal activity superior to those of the positive controls.Molecular docking was also performed to confirm the practical value of the active compound as a potential inhibitor of Leucyl-tRNA Synthetase(LeuRS).This study indicates that the designed benzoxaborole derivatives could serve as template molecules for the development of novel fungicides.
基金Supported by the National Natural Science Foundation of China and the President's Research Foundation of Academia Sinica.
文摘The chemical modification of the sulfhydryl groups of E. coli Leucyl--tRNA synthetase(LeuRS) by DTNB, NEM and IAA resulted in a time-dependent loss of both amino-acid acti-vation and aminoacylation activities in parallel. The second-order reaction constants of DTNB,NEM and IAA were 1700, 150 and 0.46 mol/L^(-1) min^(-1) respectively. Chemical stoichiometryshowed that only one sulfhydryl group of LeuRS was essential for both activities. Substratesleucine and Leu-AMP protected the active sulfhydryl group from modification, suggestingthat the modified sulfhydryl group is located in or near the active site region responsiblefor amino-acid activation. [^(14)C]NEM--labeled LeuRS was subjected to tryptic digestion, andpeptides were separated and sequenced. 179 Cys~*-Asp-Thr-Leu182 was identified as the major[^(14)C]NEM-labeled site in LeuRS. This result is consistent with the previous observationthat the region for Leu--AMP formation was located at the N--terminal part of LeuRS.
