Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function...Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function in SE. A homolog GhSERK2 （accession number： JF430801） was cloned from Upland cotton and characterized for its functions in SE. GhSERK2 expressed in different tissues and showed higher expression level in floral organs than vegetative ones with the highest levels in ovule and anther. GhSERK2 expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid （IBA）, the transcription level of GhSERK2 was induced and promoted SE subsequently. A 2-day treatment of 2,4-dichlorophenoxyacetic acid （2,4-D） induced the expression of GhSERK2, but treatments of 2,4-D for longer periods sharply inhibited the GhSERK2 transcription level of embryogenic callus （EC）. The levels of hormones, including 3-indoleacetic acid （IAA）, abscisic acid （ABA）, and brassinosteroid （BR）, were increased in the initial calli induced from the over-expression of GhSERK2 cotton. Our results indicated that GhSERK2 expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton.展开更多
[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract ...[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract of F.potentilla(MEE)was administered orally to the T2DM rats,and the level of glucose and expression of insulin,resistin,leptin,glucokinase(GK)and glucose-6-phosphatase(G-6-Pase)in the rats were measured and detected.[Results]In the T2DM rats administered with MEE,the postprandial blood glucose level decreased,the expression levels of resistin,leptin and G-6-Pase were down-regulated,and the expression levels of insulin and GK were up-regulated(P<0.05,P<0.01).[Conclusions]F.potentilla can reduce effectively postprandial blood glucose level,regulate the expression of a variety of enzymes and hormones related to glucose and lipid metabolism and recover partially the insulin level in T2DM rats to achieve resistance to T2DM.展开更多
This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two C...This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.展开更多
为了减少多核处理器系统全局调度算法中共享L2cache抖动,在Pfair调度算法基础上提出一种新的Cache感知的软实时公平调度算法.通过对WSS(work set size)、子任务截止时间和任务负载建立多因素优先级模型,并将此优先级模型应用到改进后的P...为了减少多核处理器系统全局调度算法中共享L2cache抖动,在Pfair调度算法基础上提出一种新的Cache感知的软实时公平调度算法.通过对WSS(work set size)、子任务截止时间和任务负载建立多因素优先级模型,并将此优先级模型应用到改进后的Pfair算法中,该算法的调度决策在系统负载较重的系统中对WSS具有自适应性.模拟实验结果显示:在对称4核和8核处理器系统中,该算法任务丢失率低,且在系统负载重时能够减少共享L2cache抖动,其整体调度性能优于传统的G-EDF(global-earliest deadline first)调度算法和Pfair调度算法.展开更多
基金supported in part by the National Natural Science Foundation of China (31371666)a grant from the National Key Specific Program to Hua Jinping (2016ZX08005-003)
文摘Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function in SE. A homolog GhSERK2 （accession number： JF430801） was cloned from Upland cotton and characterized for its functions in SE. GhSERK2 expressed in different tissues and showed higher expression level in floral organs than vegetative ones with the highest levels in ovule and anther. GhSERK2 expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid （IBA）, the transcription level of GhSERK2 was induced and promoted SE subsequently. A 2-day treatment of 2,4-dichlorophenoxyacetic acid （2,4-D） induced the expression of GhSERK2, but treatments of 2,4-D for longer periods sharply inhibited the GhSERK2 transcription level of embryogenic callus （EC）. The levels of hormones, including 3-indoleacetic acid （IAA）, abscisic acid （ABA）, and brassinosteroid （BR）, were increased in the initial calli induced from the over-expression of GhSERK2 cotton. Our results indicated that GhSERK2 expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton.
基金Supported by National Natural Science Foundation of China(No.81460652)Key Research and Development and Achievements Transformation Program of Qinghai Province(No.2018-SF-144)Project Funded by Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibet Plateau in Qinghai Province(No.2017-z-y25)
文摘[Objectives]To study the effects of Fruticosa potentilla L.on the expression of key enzymes and hormones related to glucose and lipid metabolism in T2DM rats.[Methods]The ethyl acetate fraction of 95%methanol extract of F.potentilla(MEE)was administered orally to the T2DM rats,and the level of glucose and expression of insulin,resistin,leptin,glucokinase(GK)and glucose-6-phosphatase(G-6-Pase)in the rats were measured and detected.[Results]In the T2DM rats administered with MEE,the postprandial blood glucose level decreased,the expression levels of resistin,leptin and G-6-Pase were down-regulated,and the expression levels of insulin and GK were up-regulated(P<0.05,P<0.01).[Conclusions]F.potentilla can reduce effectively postprandial blood glucose level,regulate the expression of a variety of enzymes and hormones related to glucose and lipid metabolism and recover partially the insulin level in T2DM rats to achieve resistance to T2DM.
基金Project supported by the Central Public Research Institute Basic Fund for Research and Development (2008-jxh-1),Agro-environmental Protection Institute,Ministry of Agriculture,China
文摘This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.
基金National Natural Science Foundation of China under Grant Nos.10535010 and 10775068the State Key Basic Research Program under Grant No.2007CB815004+1 种基金the CAS Knowledge Innovation Project under Grant No.KJCX2-SW-N02the Research Fund of High Education under Grant No.20010284036
文摘我们学习 N = 的水平结构在单个粒子的潜力的框架的 9 等渗性和他们的镜子原子核建模的 7 ~ 。就常规潜在模型的计算的限制而言，联合的 isospin 依赖的 l <SUP>2</SUP> 最新在平均潜力被介绍。修改模型为所有学习原子核的结构给统一描述。计算自我一致地在 N = 生产 s-d 水平倒置 9 等渗性和他们的镜子原子核。同时，在镜子原子核 <SUP>11</SUP 的 s-p 水平倒置 > 并且 <SUP>11</SUP > N 被复制。学习在 <SUP>11</SUP 证实中子光圈结构 >(2s <SUB>1/2</SUB>),<SUP>11</SUP >(1p <SUB>1/2</SUB>),<SUP>12</SUP > B (2s <SUB>1/2</SUB>),<SUP>14</SUP > B (2s <SUB>1/2</SUB>),<SUP>13</SUP > C (2s <SUB>1/2</SUB>),<SUP>15</SUP > C (在 <SUP>17</SUP 的 2s <SUB>1/2</SUB>) 和质子光圈结构 > F (2s <SUB>1/2</SUB>) 。在理论和实验之间的协议显示联合的 l <SUP>2</SUP> 的包括是解释异国情调的轻原子核的反常结构的一个可行方法。
文摘为了减少多核处理器系统全局调度算法中共享L2cache抖动,在Pfair调度算法基础上提出一种新的Cache感知的软实时公平调度算法.通过对WSS(work set size)、子任务截止时间和任务负载建立多因素优先级模型,并将此优先级模型应用到改进后的Pfair算法中,该算法的调度决策在系统负载较重的系统中对WSS具有自适应性.模拟实验结果显示:在对称4核和8核处理器系统中,该算法任务丢失率低,且在系统负载重时能够减少共享L2cache抖动,其整体调度性能优于传统的G-EDF(global-earliest deadline first)调度算法和Pfair调度算法.
