Objective:To investigate the effects of Astragalus Injection on inflammatory factors, chemokines, renal function and humoral immunity in patients with pulmonary tuberculosis. Methods:80 patients with pulmonary tubercu...Objective:To investigate the effects of Astragalus Injection on inflammatory factors, chemokines, renal function and humoral immunity in patients with pulmonary tuberculosis. Methods:80 patients with pulmonary tuberculosis who were treated in the department of respiratory medicine in our hospital from October 2015 to October 2017 were randomly divided into control group and observation group, 40 cases in each group. Patients in the control group were given levofloxacin treatment;and patients in the observation group were given astragalus injection combined with levofloxacin treatment. Before and after treatment, procalcitonin (PCT), interferon-γ (INF-γ), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1 ), blood urea nitrogen (BUN), serum creatinine (SCr), uric acid (UA) and immunoglobulin (IgA, IgM, IgG) levels were measured and compared between the patients in the two groups.Result: After treatment, the levels of PCT, INF-γ, MCP-1 and MIP-1 in serum of the patients in the two groups were significantly decreased, and the levels of IgA, IgM and IgG were significantly increased. The changes of the PCT, INF-γ, MCP-1, MIP-1 , IgA, IgM and IgG of patients in the observation group were significantly stronger than those in the control group (P<0.05). After treatment, the levels of BUN, SCr and UA in serum of patients in the two groups increased significantly. The serum levels of above indexes of patients in the observation group were significantly lower than those in the control group (P<0.05).Conclusion: Astragalus injection can significantly relieve the inflammatory state of patients with pulmonary tuberculosis, reduce the level of chemokines, enhance the renal function and immune function of patients. It has good clinical efficacy.展开更多
The general strategy and method of constructing universal calibration model for levofioxacin injections by near-infrared spectroscopy have been investigated and discussed. Firstly, a constant-temperature homogeneous l...The general strategy and method of constructing universal calibration model for levofioxacin injections by near-infrared spectroscopy have been investigated and discussed. Firstly, a constant-temperature homogeneous liquid calibration model for levofloxacin hydrochloride injections with the same composition but different active principal ingredient （API） content was established as the basic unit for universal model. Then, samples of levofloxacin hydrochloride injections containing propylene glycol or levofloxacin lactate injections were added to develop a primary constant-temperature liquid universal model. Temperature- amended final universal model was established to apply to samples under different temperatures. The final model was built from 61 calibration samples and 77 validation samples. The value of the root mean square error of cross validation （RMSECV） and coefficient of determination （r2） of leave-one-out cross-validation （LOOCV） were 0.792 and 0.9993, respectively, the root mean square error of prediction （RMSEP） of test set validation （TSV） was 0.87, and the average relative deviation was 1.44%. According to the ICH guidelines, the universal calibration model was evaluated. Based on the experimental statistical results, the recommended number of calibration samples for a constant-temperature homogeneous liquid quantitative model was no less than 15.展开更多
文摘Objective:To investigate the effects of Astragalus Injection on inflammatory factors, chemokines, renal function and humoral immunity in patients with pulmonary tuberculosis. Methods:80 patients with pulmonary tuberculosis who were treated in the department of respiratory medicine in our hospital from October 2015 to October 2017 were randomly divided into control group and observation group, 40 cases in each group. Patients in the control group were given levofloxacin treatment;and patients in the observation group were given astragalus injection combined with levofloxacin treatment. Before and after treatment, procalcitonin (PCT), interferon-γ (INF-γ), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1 ), blood urea nitrogen (BUN), serum creatinine (SCr), uric acid (UA) and immunoglobulin (IgA, IgM, IgG) levels were measured and compared between the patients in the two groups.Result: After treatment, the levels of PCT, INF-γ, MCP-1 and MIP-1 in serum of the patients in the two groups were significantly decreased, and the levels of IgA, IgM and IgG were significantly increased. The changes of the PCT, INF-γ, MCP-1, MIP-1 , IgA, IgM and IgG of patients in the observation group were significantly stronger than those in the control group (P<0.05). After treatment, the levels of BUN, SCr and UA in serum of patients in the two groups increased significantly. The serum levels of above indexes of patients in the observation group were significantly lower than those in the control group (P<0.05).Conclusion: Astragalus injection can significantly relieve the inflammatory state of patients with pulmonary tuberculosis, reduce the level of chemokines, enhance the renal function and immune function of patients. It has good clinical efficacy.
基金National Science and Technology Major Project of the Ministry of Science and Technology of China(Grant No. 2010ZX09401-403)
文摘The general strategy and method of constructing universal calibration model for levofioxacin injections by near-infrared spectroscopy have been investigated and discussed. Firstly, a constant-temperature homogeneous liquid calibration model for levofloxacin hydrochloride injections with the same composition but different active principal ingredient （API） content was established as the basic unit for universal model. Then, samples of levofloxacin hydrochloride injections containing propylene glycol or levofloxacin lactate injections were added to develop a primary constant-temperature liquid universal model. Temperature- amended final universal model was established to apply to samples under different temperatures. The final model was built from 61 calibration samples and 77 validation samples. The value of the root mean square error of cross validation （RMSECV） and coefficient of determination （r2） of leave-one-out cross-validation （LOOCV） were 0.792 and 0.9993, respectively, the root mean square error of prediction （RMSEP） of test set validation （TSV） was 0.87, and the average relative deviation was 1.44%. According to the ICH guidelines, the universal calibration model was evaluated. Based on the experimental statistical results, the recommended number of calibration samples for a constant-temperature homogeneous liquid quantitative model was no less than 15.
