A PCR-based homologous cloning strategy was used to identify an actin gene from the roots of Chinese licorice（Glycyrrhiza uralensis Fisch）. Results of sequence analysis indicate that a 1137 bp cDNA with an open read...A PCR-based homologous cloning strategy was used to identify an actin gene from the roots of Chinese licorice（Glycyrrhiza uralensis Fisch）. Results of sequence analysis indicate that a 1137 bp cDNA with an open reading frame encoding 377 amino acids, actin ortholog, GuActin, was successfully cloned and characterized（GenBank accession No. EU190972）. Thus far, GuActin is the first actin of Chinese licorice that has been identified at a molecular level. Analysis by Northern blot shows that GuActin was expressed strongly in the roots, particularly in radicles than in stems and leaves. These results suggest that GuActin may be a member of the vegetative subfamily of the actin family.展开更多
Low energy ion beams are known to have stimulation effects on plant generation and to improve plants＇ intrinsic quality. In the present study, the growth and physiological index of licorice implanted with 0, 8, 10; 1...Low energy ion beams are known to have stimulation effects on plant generation and to improve plants＇ intrinsic quality. In the present study, the growth and physiological index of licorice implanted with 0, 8, 10; 12 and 14× （2.6× 10^15） ions/cm^2 were investigated under well-watered and drought-stress conditions. The results showed that a proper dose of ion implan- tation was particularly efficient in stimulating the licorice growth and improving the plant biomass significantly in both the well-watered and drought-stress conditions. The physiological results of licorice measured by leaf water potential, lipid oxidation, soluble protein and antioxidant system showed a significant correlation between ion implantation and water regime except for leaf water potential. Therefore, the study indicated that ion implantation can enhance licorice＇s drought tolerance by increasing the activity of superoxide dismutase （SOD）, eatalase （CAT） and DPPH （1,1-diphenyl-2-picrylhydrazyl） radical scavenging ability to lower oxidative damage to lipids in plants. Ion beam implantation, therefore, provides an alternative method to enhance licorice drought tolerance.展开更多
Pseudo-allergic reactions(PARs)widely occur upon application of drugs or functional foods.Anti-pseudo-allergic ingredients from natural products have attracted much attention.This study aimed to investigate anti-pseud...Pseudo-allergic reactions(PARs)widely occur upon application of drugs or functional foods.Anti-pseudo-allergic ingredients from natural products have attracted much attention.This study aimed to investigate anti-pseudo-allergic compounds in licorice.The anti-pseudo-allergic effect of licorice extract was evaluated in rat basophilic leukemia 2H3(RBL-2H3)cells.Anti-pseudo-allergic compounds were screened by using RBL-2H3 cell extraction and the effects of target components were verified further in RBL-2H3 cells,mouse peritoneal mast cells(MPMCs)and mice.Molecular docking and human MRGPRX2-expressing HEK293T cells(MRGPRX2-HEK293T cells)extraction were performed to determine the potential ligands of MAS-related G protein-coupled receptor-X2(MRGPRX2),a pivotal target for PARs.Glycyrrhizic acid(GA)and licorice chalcone A(LA)were screened and shown to inhibit Compound48/80-induced degranulation and calcium influx in RBL-2H3 cells.GA and LA also inhibited degranulation in MPMCs and increase of histamine and TNF-a in mice.LA could bind to MRGPRX2,as determined by molecular docking and MRGPRX2-HEK293T cell extraction.Our study provides a strong rationale for using GA and LA as novel treatment options for PARs.LA is a potential ligand of MRGPRX2.展开更多
Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components on the Nav1.4 voltage-gated sodium channels(VGSCs).Materials and Methods:W...Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components on the Nav1.4 voltage-gated sodium channels(VGSCs).Materials and Methods:Writhing test was carried out with ICR mice.Paeonia lactiflora and Glycyrrhiza uralensis group were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 2.94 mg/ml,1.47 mg/ml,and 0.74 mg/ml using intragastric administration,respectively.Peony and licorice decoction groups were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 5.89 mg/ml,2.94 mg/ml,and 1.47 mg/ml using intragastric administration,respectively.For electrophysiology studies,each freeze-dried powder was dissolved in DMSO to make 10 mg/ml and 50 mg/ml stock solutions.The electrophysiological recordings were obtained under visual control of a microscope.For UPLC analysis,the freeze-dried powder was dissolved in methanol and then determines the contents of the nine marker compounds.Results:The effect of G.uralensis on incubation period and writhing frequency was significantly better than that of peony and licorice decoction group and P.lactiflora group.The inhibition rate of 50 mg/ml water extracts of the three samples was significantly higher than that of the 10 mg/ml group.Moreover,the water extract of G.uralensis at 50 mg/ml had the strongest inhibitory effect on I_(Nav) 1.4 of the three.Conclusion:The possible mechanism of peony and licorice decoction in relieving spasm and pain is most likely by inhibiting Voltage-Gated Sodium Channel Subtype 1.4.展开更多
基金Supported by the Natural Science Foundation of Fujian Province, China(No.200804011)the Special Foundation for Young Scientists of Fujian Province, China(No.2006F3113)+1 种基金Scientific Research Foundation of Educational Committee of Fujian Province, China(No.JA07151) Technology Research and Development Program of Quanzhou’ Science and Technology Bureau, China(No.2007N6)
文摘A PCR-based homologous cloning strategy was used to identify an actin gene from the roots of Chinese licorice（Glycyrrhiza uralensis Fisch）. Results of sequence analysis indicate that a 1137 bp cDNA with an open reading frame encoding 377 amino acids, actin ortholog, GuActin, was successfully cloned and characterized（GenBank accession No. EU190972）. Thus far, GuActin is the first actin of Chinese licorice that has been identified at a molecular level. Analysis by Northern blot shows that GuActin was expressed strongly in the roots, particularly in radicles than in stems and leaves. These results suggest that GuActin may be a member of the vegetative subfamily of the actin family.
基金supported by the grants from the Chinese National High-Technology Program(Nos.2002AA327070,2004AA32G060)the Natural Science Foundation of Education Department of Anhui Province(No.2005KJ152)
文摘Low energy ion beams are known to have stimulation effects on plant generation and to improve plants＇ intrinsic quality. In the present study, the growth and physiological index of licorice implanted with 0, 8, 10; 12 and 14× （2.6× 10^15） ions/cm^2 were investigated under well-watered and drought-stress conditions. The results showed that a proper dose of ion implan- tation was particularly efficient in stimulating the licorice growth and improving the plant biomass significantly in both the well-watered and drought-stress conditions. The physiological results of licorice measured by leaf water potential, lipid oxidation, soluble protein and antioxidant system showed a significant correlation between ion implantation and water regime except for leaf water potential. Therefore, the study indicated that ion implantation can enhance licorice＇s drought tolerance by increasing the activity of superoxide dismutase （SOD）, eatalase （CAT） and DPPH （1,1-diphenyl-2-picrylhydrazyl） radical scavenging ability to lower oxidative damage to lipids in plants. Ion beam implantation, therefore, provides an alternative method to enhance licorice drought tolerance.
基金This work was supported by the National Natural Scientific Foundation of China(No.81903788 and 81900780).
文摘Pseudo-allergic reactions(PARs)widely occur upon application of drugs or functional foods.Anti-pseudo-allergic ingredients from natural products have attracted much attention.This study aimed to investigate anti-pseudo-allergic compounds in licorice.The anti-pseudo-allergic effect of licorice extract was evaluated in rat basophilic leukemia 2H3(RBL-2H3)cells.Anti-pseudo-allergic compounds were screened by using RBL-2H3 cell extraction and the effects of target components were verified further in RBL-2H3 cells,mouse peritoneal mast cells(MPMCs)and mice.Molecular docking and human MRGPRX2-expressing HEK293T cells(MRGPRX2-HEK293T cells)extraction were performed to determine the potential ligands of MAS-related G protein-coupled receptor-X2(MRGPRX2),a pivotal target for PARs.Glycyrrhizic acid(GA)and licorice chalcone A(LA)were screened and shown to inhibit Compound48/80-induced degranulation and calcium influx in RBL-2H3 cells.GA and LA also inhibited degranulation in MPMCs and increase of histamine and TNF-a in mice.LA could bind to MRGPRX2,as determined by molecular docking and MRGPRX2-HEK293T cell extraction.Our study provides a strong rationale for using GA and LA as novel treatment options for PARs.LA is a potential ligand of MRGPRX2.
基金financial supports from "Study on the Development of Classical Prescriptions of Peony and Liquorice Decoction"(NO:H2016072-03)"Study on the Standard Decoction of Traditional Chinese Medicinal Slices"(NO:H2016021-06)
文摘Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components on the Nav1.4 voltage-gated sodium channels(VGSCs).Materials and Methods:Writhing test was carried out with ICR mice.Paeonia lactiflora and Glycyrrhiza uralensis group were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 2.94 mg/ml,1.47 mg/ml,and 0.74 mg/ml using intragastric administration,respectively.Peony and licorice decoction groups were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 5.89 mg/ml,2.94 mg/ml,and 1.47 mg/ml using intragastric administration,respectively.For electrophysiology studies,each freeze-dried powder was dissolved in DMSO to make 10 mg/ml and 50 mg/ml stock solutions.The electrophysiological recordings were obtained under visual control of a microscope.For UPLC analysis,the freeze-dried powder was dissolved in methanol and then determines the contents of the nine marker compounds.Results:The effect of G.uralensis on incubation period and writhing frequency was significantly better than that of peony and licorice decoction group and P.lactiflora group.The inhibition rate of 50 mg/ml water extracts of the three samples was significantly higher than that of the 10 mg/ml group.Moreover,the water extract of G.uralensis at 50 mg/ml had the strongest inhibitory effect on I_(Nav) 1.4 of the three.Conclusion:The possible mechanism of peony and licorice decoction in relieving spasm and pain is most likely by inhibiting Voltage-Gated Sodium Channel Subtype 1.4.
