Objective To explore the therapeutic efficacy and mechanism of acupuncture at Lieque (列缺 LU 7) in treatment of cervical vertigo (CV). Methods Forty CV patients met the inclusion criteria were enrolled and treate...Objective To explore the therapeutic efficacy and mechanism of acupuncture at Lieque (列缺 LU 7) in treatment of cervical vertigo (CV). Methods Forty CV patients met the inclusion criteria were enrolled and treated with acupuncture at bilateral LU 7 points, once daily with 10 times as a course. The changes of blood flow velocity in bilateral vertebral artery and basilar artery were observed through transcranial doppler sonography (TCD) before treatment, after deqi with acupuncture and after a course of treatment, respectively. The scores and therapeutic efficacy on clinical symptoms were assessed according to CV symptoms and the functional assessment scale as well as the Criteria-for Diagnosis and Curative Effect in TCM Syndromes before treatment and after a course of treatment. Results Before treatment, after deqi and after a course of treatment, the blood flow velocity of basilar artery (BA), left vertebral artery (LVA) and right vertebral artery (RVA) in 22 patients with a decreased blood flow velocity were respectively 23.20±4.84 vs 26.30 ± 4.17 vs 29.20 ± 4.20 (mm/s, BA), 21.65 ± 3.62 vs 24.20 ± 2.89 vs 26.40 ± 3.62 (mm/s, LVA) and 21.90±3.04 vs 24.25±3.01 vs 26.50_±3.95 (mm/s, RVA), while in 18 patients with an increased blood flow velocity were respectively 39.94 ± 8.24 vs 35.17 ± 4.84 vs 32.06 ± 3.49 (mm/s, BA), 41.83 ± 5.64 vs 37.28 ± 2.32 vs 35.61 ± 2.09 (mm/s, LVA) and 37.11 ± 9.83 vs 32.22 ± 6.13 vs 28.11 ± 4.12 (mm/s, RVA). Except that the difference of blood flow velocity of RVA was not significant in patients with an increased blood flow velocity before treatment and after deqi (P 〉 0.05), the Vm of all vessels in 40 patients was improved after deqi with acupuncture and a course of treatment (P〈0.01, P〈0.05). According to CV symptoms and the functional assessment scale, before treatment and after a course of treatment, the scores were 15.68 ± 5.35 and 26.30 ± 3.76, respectively, indicating that after a course of treatment, the symptoms were significantly improved (P 〈 0.01). According to the Criteria for Diagnosis and Curative E.f]ect in TCM Syndromes, after a course of treatment, the total effective rate was 100% (40/40) and the cured rate was 55.0% (22/40). Conclusion Acupuncture at LU 7 not only improves the blood supply of vertebral-basilar artery in CV patients, but also has a significant curative effect on improving the clinical symptoms.展开更多
Objective:Pain is a health problem frequently reported in the clinical and general populations.Acupoint therapy is one of the most effective ways to treat pain;however,its mechanism remains unclear.Therefore,this stud...Objective:Pain is a health problem frequently reported in the clinical and general populations.Acupoint therapy is one of the most effective ways to treat pain;however,its mechanism remains unclear.Therefore,this study aimed to explore the relationship between distal acupoints,dorsal root ganglion(DRG)neurons,and spinal cord dorsal horn(SDH) neurons in neck acute inflammatory pain(NAIP).Methods:NAIP model rats were used to explore the relationship between acupoint sensitization and pain.Out of fourteen rats,ten rats were grouped into control and NAIP groups,five rats in control and five rats in NAIP.Mustard oil was subcutaneously injected on one side between the C4 and C7 vertebrae of the neck to establish an NAIP model.Evans blue(EB) was injected through the tail vein to detect sensitized acupoints after NAIP modeling.EB exudation in the body,Liè qūe(列缺 LU7),and Língdào(灵道 HT4) were evaluated.An immunofluorescence assay was conducted to detect the expression of calcitonin generelated peptide(CGRP),isolectin B4(IB4),and c-Fos in the dorsal root ganglion(DRG)and spinal cord dorsal medullae spinalis(CDMS).Four rats were used for the retrograde labeling of neurons of the LU7 region to the DRG and CDMS using CTB-488 and CTB-555 microinjection.Results:NAIP was shown to lead to oozing and pain sensitization in the LU7 and HT4 and increased the proportion of c-Fos^(+)/CGRP^(+)and c-Fos^(+)/IB4^(+)cells in both the DRG and CDMS.CTB-488 and CTB-555injected into the LU7 and sensitization point areas were observed in the DRG and CDMS regions of NAIP rats.Conclusions:Our study revealed that NAIP could lead to oozing and pain sensitization in the LU7 and HT4regions and that the pain point around LU7 might result from the transfer of peptidergic(CGRP-positive)and non-peptidergic(IB4-positive) neurons in the DRG and CDMS.展开更多
Objective:To observe the effect of Zhen'ai needling method in Nei Jing (Classic of Internal Medicine) on quality of life in patients with allergic rhinitis (AR).Methods:A total of 99 patients with AR who met the i...Objective:To observe the effect of Zhen'ai needling method in Nei Jing (Classic of Internal Medicine) on quality of life in patients with allergic rhinitis (AR).Methods:A total of 99 patients with AR who met the inclusion criteria were randomized into a conventional needling group (51 cases) and a Zhen'ai needling group (48 cases).The conventional needling group was treated with acupuncture at Zusanli (ST 36),Hegu (LI 4),Juliao (ST 3),Yingxiang (LI 20),Yintang (GV 29),Shangxing (GV 23) and Baihui (GV 20).The Zhen'ai needling group added acupoints of Zhen'ai needling method {Lieque (LU 7),Shanglianquan [Extra,located at 1 cun above Lianquan (CV 23)] and Tianrong (SI 17)} in addition to points in the conventional needling group.The needles were retained for 30 min.The treatment was performed twice a week.All patients were assessed by total nasal symptom score (TNSS),total ocular symptom score (TOSS) and sino-nasal outcome test-20 (SNOT-20) before and after 15 treatments to evaluate the quality of life.The clinical efficacy was evaluated after 15 treatments.Results:During the treatment,8 cases dropped out in the conventional needling group and 7 cases in the Zhen'ai needling group.There were no significant differences in TNSS,TOSS and SNOT-20 scores between the two groups before treatment (all P>0.05).After treatment,the total effective rate of the Zhen'ai needling group was 92.7%,versus 88.4% in the conventional needling group.There was no significant difference between the two groups (P>0.05).After treatment,there were no significant differences between the two groups in TNSS and TOSS scores (both P>0.05).The SNOT-20 score of the Zhen'ai needling group was lower than that of the conventional needling group,and the difference was statistically significant (P<0.05).There were statistical differences between the two groups in the scores of cough and nasal reflux (to the throat) of the NOT-20 scale (both P<0.05).Conclusion:Both Zhen'ai needling method and conventional acupuncture are effective in the treatment of AR.Zhen'ai needling method has a better effect than conventional acupuncture in improving cough and nasal reflux (to the throat) in AR patients.展开更多
Objective:To observe the clinical efficacy of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis(AR)accompanied by adenoid hypertrophy(AH).Methods:A total of 74 ch...Objective:To observe the clinical efficacy of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis(AR)accompanied by adenoid hypertrophy(AH).Methods:A total of 74 children who met the screening criteria were divided into a Zhen'ai group and a control group by the random number table method,with 37 cases in each group.The control group was treated with acupuncture at Zusanli(ST 36),Hegu(LI 4),Yingxiang(LI 20),Juliao(ST 3),Yintang(GV 29),Shangxing(GV 23)and Baihui(GV 20).The Zhen'ai group added points of Zhen'ai needling method{Shanglianquan[Extra,located at 1 cun above Lianquan(CV 23)],Tianrong(SI 17)and Lieque(LU 7)}in addition to the points in the control group.The needles were retained for 30 min.The treatment was performed twice a week.The total nasal symptom score(TNSS),sino-nasal outcome test-20(SNOT-20)and symptom scale for AH(SSAH)were assessed before and after 10 treatments.The clinical efficacy of the two groups was compared after treatment.Results:During the treatment,5 cases dropped out in the control group and 2 cases in the Zhen'ai group.After treatment,the total effective rate of the Zhen'ai group was 94.3%,versus 93.8%in the control group.There was no significant difference between the two groups(P>0.05).The markedly effective rate of the Zhen'ai group was 42.9%,versus 12.5%in the control group,and the difference was statistically significant(P<0.05).After treatment,there were significant intra-group differences in the scores of TNSS,SNOT-20 and SSAH in both groups(all P<0.05);the scores of SNOT-20 and SSAH in the Zhen'ai group were lower than those in the control group,and the differences between the groups were statistically significant(both P<0.05).Conclusion:Both conventional acupuncture and conventional acupuncture plus Zhen'ai needling method can improve clinical symptoms of children with AR accompanied by AH;and conventional acupuncture plus Zhen'ai needling method has a better effect than conventional acupuncture in improving AH symptoms.展开更多
Acupuncture anesthesia is a technique by partially or completely replacing anesthetics with acupuncture in surgery based on the traditional acupuncture analgesia mechanism.It emerged in the 1950s,went viral in the 197...Acupuncture anesthesia is a technique by partially or completely replacing anesthetics with acupuncture in surgery based on the traditional acupuncture analgesia mechanism.It emerged in the 1950s,went viral in the 1970s and then gradually fell into decline.In the recent years,this technique has regained attention and further research.Acupuncture anesthesia can be classified as either pure acupuncture anesthesia or acupuncture-medication combined anesthesia.To expand the application of this technique,a patient with non-ST elevation acute coronary syndrome in urgent need of percutaneous coronary intervention (PCI) received pure acupuncture anesthesia because of an allergy to lidocaine,and the operation went successfully.This is the first time that pure acupuncture anesthesia and coronary arteriography were combined,which is of great significance in further study and development of acupuncture anesthesia.展开更多
OBJECTIVE:To investigate the impact of electro-acupuncture at the Neiguan(PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2(ASIC2) and ASIC3 in myocardial ischemia rats.METHODS:Fifty male Spra...OBJECTIVE:To investigate the impact of electro-acupuncture at the Neiguan(PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2(ASIC2) and ASIC3 in myocardial ischemia rats.METHODS:Fifty male Sprague-Dawley rats were used,weighing(230 ± 50) g.The rats were randomized into a normal group A,model group B,Neiguan(PC 6) group C,Lieque(LU 7) group D,and A-shi points group E.There were 10 rats in each group.Rats were continuously administered 85 mg/kg intravenous isoproterenol daily to establish the model.Successfully modeled rats in groups C,D,and E were given electro-acupuncture treatment.Each group of rats was sacrificed with chloral hydrate(1 mL/100 g) intraperitoneal injection.The left ventricular myocardium was extracted and placed at- 70 ℃ until use.Western blot analysis and real-time PCR were performed to assay protein and RNA expressions of ASIC2 and ASIC3,respectively.Fold changes in RNA expression were quantified with the 2~^(-△△Ct) method.Blood samples were drawn from the aorta abdominalis and tested for creatine kinase-MB(CK-MB) and lactate dehydrogenase(LDH) levels using enzyme-linked immunosorbent assay.RESULTS:Myocardial ischemia rats given electro-acupuncture at the Neiguan(PC 6) acupoint had significantly lower protein and RNA expression of ASIC2 and ASIC3,and CK-MB and LDH levels,compared with model rats(P < 0.01).CONCLUSION:Electro-acupuncture at the Neiguan(PC 6) acupoint can not only decrease the protein and RNA expression of ASIC2 and ASIC3,but also inhibit the opening of ASICs and reduce the cardiomyocyte damage in myocardial ischemia rats.展开更多
Insomnia prevalence may reach up to 30%of the population considering at least one of the following criteria:difficulty in falling asleep,difficulty in remaining asleep,waking up too early,not repairing sleep or bad sl...Insomnia prevalence may reach up to 30%of the population considering at least one of the following criteria:difficulty in falling asleep,difficulty in remaining asleep,waking up too early,not repairing sleep or bad sleep quality,followed by daily consequences of lack of sleep such as lack of attention or stress.Several studies have reported the use of acupuncture as a therapeutic alternative for insomnia treatment showing important results such as anxiety decrease,improvement of sleep quality,decrease of daily consequences of insomnia,among others.The aim of this project was to use acupuncture as treatment for insomnia using the points Shenmen(神门HT7),Taiyuan(太渊LU9),Neiguan(内关PC6)and Yintang(印堂EX-HN3).After the signing of the TCLE,the patient answered a questionnaire during sessions 1,5 and10.The patient received insomnia treatment through puncture of the following points:HT7,LU9,PC6 and EX-HN3.The patient reported a significant improve in the sleep quality after the first sessions and absence of the symptoms after the tenth session.Therefore we conclude that the stimulation of these acupoints is useful in the insomnia treatment.展开更多
文摘Objective To explore the therapeutic efficacy and mechanism of acupuncture at Lieque (列缺 LU 7) in treatment of cervical vertigo (CV). Methods Forty CV patients met the inclusion criteria were enrolled and treated with acupuncture at bilateral LU 7 points, once daily with 10 times as a course. The changes of blood flow velocity in bilateral vertebral artery and basilar artery were observed through transcranial doppler sonography (TCD) before treatment, after deqi with acupuncture and after a course of treatment, respectively. The scores and therapeutic efficacy on clinical symptoms were assessed according to CV symptoms and the functional assessment scale as well as the Criteria-for Diagnosis and Curative Effect in TCM Syndromes before treatment and after a course of treatment. Results Before treatment, after deqi and after a course of treatment, the blood flow velocity of basilar artery (BA), left vertebral artery (LVA) and right vertebral artery (RVA) in 22 patients with a decreased blood flow velocity were respectively 23.20±4.84 vs 26.30 ± 4.17 vs 29.20 ± 4.20 (mm/s, BA), 21.65 ± 3.62 vs 24.20 ± 2.89 vs 26.40 ± 3.62 (mm/s, LVA) and 21.90±3.04 vs 24.25±3.01 vs 26.50_±3.95 (mm/s, RVA), while in 18 patients with an increased blood flow velocity were respectively 39.94 ± 8.24 vs 35.17 ± 4.84 vs 32.06 ± 3.49 (mm/s, BA), 41.83 ± 5.64 vs 37.28 ± 2.32 vs 35.61 ± 2.09 (mm/s, LVA) and 37.11 ± 9.83 vs 32.22 ± 6.13 vs 28.11 ± 4.12 (mm/s, RVA). Except that the difference of blood flow velocity of RVA was not significant in patients with an increased blood flow velocity before treatment and after deqi (P 〉 0.05), the Vm of all vessels in 40 patients was improved after deqi with acupuncture and a course of treatment (P〈0.01, P〈0.05). According to CV symptoms and the functional assessment scale, before treatment and after a course of treatment, the scores were 15.68 ± 5.35 and 26.30 ± 3.76, respectively, indicating that after a course of treatment, the symptoms were significantly improved (P 〈 0.01). According to the Criteria for Diagnosis and Curative E.f]ect in TCM Syndromes, after a course of treatment, the total effective rate was 100% (40/40) and the cured rate was 55.0% (22/40). Conclusion Acupuncture at LU 7 not only improves the blood supply of vertebral-basilar artery in CV patients, but also has a significant curative effect on improving the clinical symptoms.
基金Supported by the National Key Research and Development Program of China:2018YFC1704606。
文摘Objective:Pain is a health problem frequently reported in the clinical and general populations.Acupoint therapy is one of the most effective ways to treat pain;however,its mechanism remains unclear.Therefore,this study aimed to explore the relationship between distal acupoints,dorsal root ganglion(DRG)neurons,and spinal cord dorsal horn(SDH) neurons in neck acute inflammatory pain(NAIP).Methods:NAIP model rats were used to explore the relationship between acupoint sensitization and pain.Out of fourteen rats,ten rats were grouped into control and NAIP groups,five rats in control and five rats in NAIP.Mustard oil was subcutaneously injected on one side between the C4 and C7 vertebrae of the neck to establish an NAIP model.Evans blue(EB) was injected through the tail vein to detect sensitized acupoints after NAIP modeling.EB exudation in the body,Liè qūe(列缺 LU7),and Língdào(灵道 HT4) were evaluated.An immunofluorescence assay was conducted to detect the expression of calcitonin generelated peptide(CGRP),isolectin B4(IB4),and c-Fos in the dorsal root ganglion(DRG)and spinal cord dorsal medullae spinalis(CDMS).Four rats were used for the retrograde labeling of neurons of the LU7 region to the DRG and CDMS using CTB-488 and CTB-555 microinjection.Results:NAIP was shown to lead to oozing and pain sensitization in the LU7 and HT4 and increased the proportion of c-Fos^(+)/CGRP^(+)and c-Fos^(+)/IB4^(+)cells in both the DRG and CDMS.CTB-488 and CTB-555injected into the LU7 and sensitization point areas were observed in the DRG and CDMS regions of NAIP rats.Conclusions:Our study revealed that NAIP could lead to oozing and pain sensitization in the LU7 and HT4regions and that the pain point around LU7 might result from the transfer of peptidergic(CGRP-positive)and non-peptidergic(IB4-positive) neurons in the DRG and CDMS.
基金Shanghai Municipal Health and Family Planning Commission Research Project(上海市卫生和计划生育委员会科研课题,201540150)%Guidance Project of Traditional Chinese Medicine of Shanghai Science and Technology Committee(上海市科学技术委员会科研计划中医引导类项目,17401932200)
文摘Objective:To observe the effect of Zhen'ai needling method in Nei Jing (Classic of Internal Medicine) on quality of life in patients with allergic rhinitis (AR).Methods:A total of 99 patients with AR who met the inclusion criteria were randomized into a conventional needling group (51 cases) and a Zhen'ai needling group (48 cases).The conventional needling group was treated with acupuncture at Zusanli (ST 36),Hegu (LI 4),Juliao (ST 3),Yingxiang (LI 20),Yintang (GV 29),Shangxing (GV 23) and Baihui (GV 20).The Zhen'ai needling group added acupoints of Zhen'ai needling method {Lieque (LU 7),Shanglianquan [Extra,located at 1 cun above Lianquan (CV 23)] and Tianrong (SI 17)} in addition to points in the conventional needling group.The needles were retained for 30 min.The treatment was performed twice a week.All patients were assessed by total nasal symptom score (TNSS),total ocular symptom score (TOSS) and sino-nasal outcome test-20 (SNOT-20) before and after 15 treatments to evaluate the quality of life.The clinical efficacy was evaluated after 15 treatments.Results:During the treatment,8 cases dropped out in the conventional needling group and 7 cases in the Zhen'ai needling group.There were no significant differences in TNSS,TOSS and SNOT-20 scores between the two groups before treatment (all P>0.05).After treatment,the total effective rate of the Zhen'ai needling group was 92.7%,versus 88.4% in the conventional needling group.There was no significant difference between the two groups (P>0.05).After treatment,there were no significant differences between the two groups in TNSS and TOSS scores (both P>0.05).The SNOT-20 score of the Zhen'ai needling group was lower than that of the conventional needling group,and the difference was statistically significant (P<0.05).There were statistical differences between the two groups in the scores of cough and nasal reflux (to the throat) of the NOT-20 scale (both P<0.05).Conclusion:Both Zhen'ai needling method and conventional acupuncture are effective in the treatment of AR.Zhen'ai needling method has a better effect than conventional acupuncture in improving cough and nasal reflux (to the throat) in AR patients.
文摘Objective:To observe the clinical efficacy of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis(AR)accompanied by adenoid hypertrophy(AH).Methods:A total of 74 children who met the screening criteria were divided into a Zhen'ai group and a control group by the random number table method,with 37 cases in each group.The control group was treated with acupuncture at Zusanli(ST 36),Hegu(LI 4),Yingxiang(LI 20),Juliao(ST 3),Yintang(GV 29),Shangxing(GV 23)and Baihui(GV 20).The Zhen'ai group added points of Zhen'ai needling method{Shanglianquan[Extra,located at 1 cun above Lianquan(CV 23)],Tianrong(SI 17)and Lieque(LU 7)}in addition to the points in the control group.The needles were retained for 30 min.The treatment was performed twice a week.The total nasal symptom score(TNSS),sino-nasal outcome test-20(SNOT-20)and symptom scale for AH(SSAH)were assessed before and after 10 treatments.The clinical efficacy of the two groups was compared after treatment.Results:During the treatment,5 cases dropped out in the control group and 2 cases in the Zhen'ai group.After treatment,the total effective rate of the Zhen'ai group was 94.3%,versus 93.8%in the control group.There was no significant difference between the two groups(P>0.05).The markedly effective rate of the Zhen'ai group was 42.9%,versus 12.5%in the control group,and the difference was statistically significant(P<0.05).After treatment,there were significant intra-group differences in the scores of TNSS,SNOT-20 and SSAH in both groups(all P<0.05);the scores of SNOT-20 and SSAH in the Zhen'ai group were lower than those in the control group,and the differences between the groups were statistically significant(both P<0.05).Conclusion:Both conventional acupuncture and conventional acupuncture plus Zhen'ai needling method can improve clinical symptoms of children with AR accompanied by AH;and conventional acupuncture plus Zhen'ai needling method has a better effect than conventional acupuncture in improving AH symptoms.
基金National Natural Science Foundation of China(国家自然科学基金项目,81573796,81503440)%Shanghai Shenkang Hospital Development Center:Three-year Plan for Promoting Clinical Skills and Innovations in Municipal Hospitals(上海申康医院发展中心-促进市级医院临床技能与临床创新能力三年行动计划,16CR1031B)%Shanghai Clinical Cooperation Pilot Project of Chinese and Western Medicine(上海市中西医临床协作试点建设项目,ZY (2018-2020)-FWTX-1011)
文摘Acupuncture anesthesia is a technique by partially or completely replacing anesthetics with acupuncture in surgery based on the traditional acupuncture analgesia mechanism.It emerged in the 1950s,went viral in the 1970s and then gradually fell into decline.In the recent years,this technique has regained attention and further research.Acupuncture anesthesia can be classified as either pure acupuncture anesthesia or acupuncture-medication combined anesthesia.To expand the application of this technique,a patient with non-ST elevation acute coronary syndrome in urgent need of percutaneous coronary intervention (PCI) received pure acupuncture anesthesia because of an allergy to lidocaine,and the operation went successfully.This is the first time that pure acupuncture anesthesia and coronary arteriography were combined,which is of great significance in further study and development of acupuncture anesthesia.
基金Supported by National Essence Basic Research and Development 973 Program(the Effects of Meridian Specific Target Organ Response to Biological Basic Research,No.2012CB518503)
文摘OBJECTIVE:To investigate the impact of electro-acupuncture at the Neiguan(PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2(ASIC2) and ASIC3 in myocardial ischemia rats.METHODS:Fifty male Sprague-Dawley rats were used,weighing(230 ± 50) g.The rats were randomized into a normal group A,model group B,Neiguan(PC 6) group C,Lieque(LU 7) group D,and A-shi points group E.There were 10 rats in each group.Rats were continuously administered 85 mg/kg intravenous isoproterenol daily to establish the model.Successfully modeled rats in groups C,D,and E were given electro-acupuncture treatment.Each group of rats was sacrificed with chloral hydrate(1 mL/100 g) intraperitoneal injection.The left ventricular myocardium was extracted and placed at- 70 ℃ until use.Western blot analysis and real-time PCR were performed to assay protein and RNA expressions of ASIC2 and ASIC3,respectively.Fold changes in RNA expression were quantified with the 2~^(-△△Ct) method.Blood samples were drawn from the aorta abdominalis and tested for creatine kinase-MB(CK-MB) and lactate dehydrogenase(LDH) levels using enzyme-linked immunosorbent assay.RESULTS:Myocardial ischemia rats given electro-acupuncture at the Neiguan(PC 6) acupoint had significantly lower protein and RNA expression of ASIC2 and ASIC3,and CK-MB and LDH levels,compared with model rats(P < 0.01).CONCLUSION:Electro-acupuncture at the Neiguan(PC 6) acupoint can not only decrease the protein and RNA expression of ASIC2 and ASIC3,but also inhibit the opening of ASICs and reduce the cardiomyocyte damage in myocardial ischemia rats.
文摘Insomnia prevalence may reach up to 30%of the population considering at least one of the following criteria:difficulty in falling asleep,difficulty in remaining asleep,waking up too early,not repairing sleep or bad sleep quality,followed by daily consequences of lack of sleep such as lack of attention or stress.Several studies have reported the use of acupuncture as a therapeutic alternative for insomnia treatment showing important results such as anxiety decrease,improvement of sleep quality,decrease of daily consequences of insomnia,among others.The aim of this project was to use acupuncture as treatment for insomnia using the points Shenmen(神门HT7),Taiyuan(太渊LU9),Neiguan(内关PC6)and Yintang(印堂EX-HN3).After the signing of the TCLE,the patient answered a questionnaire during sessions 1,5 and10.The patient received insomnia treatment through puncture of the following points:HT7,LU9,PC6 and EX-HN3.The patient reported a significant improve in the sleep quality after the first sessions and absence of the symptoms after the tenth session.Therefore we conclude that the stimulation of these acupoints is useful in the insomnia treatment.